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Tsuzuki ku, Japan

Inomata S.,Shiseido Research Center
Journal of Oleo Science | Year: 2014

The Japanese Pharmaceutical Affairs Law distinguishes cosmetics from quasi-drugs, and specifies that they must have a mild effect on the human body and must be safe to use over the long term. Therefore, the safety of cosmetics needs to be thoroughly evaluated and confirmed, taking into account the type of cosmetic, application method, conditions of use and so on. Post-marketing surveys of customers' complaints and case reports of adverse effects are important to monitor and confirm the safety of products. Although manufacturing and marketing of cosmetics are becoming more globalized, the regulations relevant to cosmetics safety still vary from country to country. Thus, compliance with different regulations in various markets is a major issue for producers. In particular, further development of alternatives to animal testing remains an urgent global issue. ©2014 by Japan Oil Chemists' Society.


Hosoi J.,Shiseido Research Center
Journal of Applied Cosmetology | Year: 2011

It is well known that mental stress affects the body and that the skin reflects a person's mental condition. Thus, proverbs, such as "The skin is the mirror which reflects the state of the mind", "The skin is a window to the mind." have developed and scientific evidence supporting this relationship has been accumulated. Examples of the effects of stress, their mechanisms, and modulation by cosmetics are reviewed in this article.


Arimoto H.,Japan National Institute of Advanced Industrial Science and Technology | Egawa M.,Shiseido Research Center
Skin Research and Technology | Year: 2015

Background/purpose: We investigate the measurement sensitivity for the water content distribution in Near-infrared (NIR) imaging ranging up to 2 μm. Methods: To evaluate the sensitivity, our attention is focused to the light penetration depth in the skin tissue because the thickness of a layer where the water content can be varied is very thin and deep light penetration decreases the signal variation. The light penetration depths in the wavelengths of 1300, 1450, and 1920 nm are calculated theoretically based on the Monte Carlo light transport simulation. Results: It is clarified that the wavelength of 1920 nm gives the smallest penetration depth. A NIR imaging experiment is demonstrated to verify the difference of imaging sensitivity. The experiment result shows that the NIR image taken at 1920 nm gives the most sensitive measurement of the water content distribution in skin. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


Ezure T.,Shiseido Research Center | Amano S.,Shiseido Research Center
Experimental Dermatology | Year: 2010

Please cite this paper as: Increased subcutaneous adipose tissue impairs dermal function in diet-induced obese mice. Experimental Dermatology 2010; 19: 878-882. Abstract: Increment of subcutaneous adipose tissue is a risk factor for facial morphological changes, such as sagging, which may be at least partly because of the increased weight burden of accumulated fat. However, it is not clear how the increase of subcutaneous adipose tissue affects dermal structure and function. We examined this issue in HR-1 hairless mice given a high-fat diet (HFD). After having been fed with HFD for 12weeks, the mice became obese and the subcutaneous adipose tissue layer was significantly thickened, while the dermal layer became significantly thinner than that of control mice fed normal diet. However, the thickness of the dermal layer was not changed in the ear pinna, which lacks a subcutaneous adipose layer, suggesting that increase of subcutaneous adipose tissue may induce dermal changes. The number of dermal fibroblasts in the dermis was significantly reduced in obese mice, although there was no change in gene expression levels of extracellular matrix components, including collagen, hyaluronic acid synthase, fibulin5, fibrillin-1, laminin β1, matrix metalloproteinases and tissue inhibitor of metalloproteinases. Dermal elasticity was significantly decreased in obese hairless mice. These results suggest that subcutaneous adipose cells in obese mice may reduce the proliferation of dermal fibroblasts and induce a decrease of dermal thickness and elasticity. Therefore, the increment of the subcutaneous adipose layer in obese subjects may induce impairment of dermal biomechanical characteristics and promote the appearance of sagging. © 2009 John Wiley & Sons A/S.


Ezure T.,Shiseido Research Center | Amano S.,Shiseido Research Center
Journal of Investigative Dermatology | Year: 2011

Subcutaneous adipose tissue lies just beneath the dermal layer, but the interaction between the two types of tissue remains obscure. Recently, we reported that obesity is associated with decreased dermal elasticity. To investigate the mechanism of the adipose tissue/dermal interaction, fibroblasts were cocultured with small or enlarged adipocytes, using a membrane insert to prevent direct contact. Enlarged adipocytes reduced 3T3-L1 fibroblast proliferation and gene expression of collagen (I)-α1 (col (I)-α1) and elastin while increasing gene expression of matrix metalloproteinase 13 (MMP13). In contrast, small adipocytes had no such effects. These results indicate that factors secreted by enlarged adipocytes influence dermal condition. As enlarged adipocytes are known to release free fatty acids (FFAs), the effects of these acids on 3T3-L1 fibroblasts were examined. Palmitic acid decreased fibroblast proliferation, reduced gene expressions of col (I)-α1 and elastin, and increased MMP13. Similar effects were observed in human dermal fibroblasts. The influence of palmitic acid on fibroblasts was inhibited by eicosapentaenoic acid (EPA), an inhibitor of Toll-like receptors (TLRs). Furthermore, EPA inhibited the effects of enlarged adipocytes on fibroblasts in the coculture system. These data indicate that enlarged adipocytes negatively control the function of dermal fibroblasts through the activation of TLRs by secreted FFAs. © 2011 The Society for Investigative Dermatology.

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