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Kim H.,Saint Louis University | Jung B.J.,Kyung Hee University | Jung J.H.,Kyung Hee University | Kim J.Y.,Kyung Hee University | And 2 more authors.
Molecules and Cells | Year: 2012

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-α also increased the production of in-ducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-α stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation. © 2012 KSMCB.

Hee Park H.,Yonsei University | Sung Park D.,Chonnam National University | Cheol Kweon D.,Shinheung College | Bock Lee S.,Nambu University | And 3 more authors.
Applied Radiation and Isotopes | Year: 2011

The number of PET/CT tests for diagnosing and treating tumors are increasing every year, and thus it is considered necessary to compare PET/CT images among medical institutions or among different PET/CT systems within the same medical institution. We present to get the variation of standardized uptake values (SUV) among PET/CT systems, to derive an SUV calibration table that enables quantitative comparison of PET/CT systems, and to test the usefulness of the calibration table through comparing patient's SUV with the SUV calibration table. The correlation coefficients that inter-compared the calibration constants of normal patients and the calibration constants of the mean standardized uptake value with the calibration constants of the standardized uptake value of phantom are 0.88938 (P<0.0001). © 2010 Elsevier Ltd.

Byun J.,Soongsil University | Rhew S.,Soongsil University | Hwang M.,Shinheung College
Communications in Computer and Information Science | Year: 2012

The suitability of requirements that are developed and changed is a key factor to make a success of project. The key elements of requirement suitability are the objectives and constraints which are decided by scope. To ensure that requirements have suitability, our study proposes the techniques for measuring requirement suitability considering objectives, constraints and requirements. To calculate requirement suitability points, which are quantities calculated by these techniques, we develop indicators and metrics based on relationship among objectives, constraints and requirements, and propose expressions to quantify our indicators using MCDM (Multi Criteria Decision Making) techniques. An interpretation and usage of our techniques are described by statistical analysis. © 2012 Springer-Verlag.

Lee C.-J.,Dankook University | Lee C.-J.,Shinheung College | Bok S.-B.,Dankook University | Bae J.-Y.,Dankook University | Lee H.-H.,Dankook University
Dental Materials Journal | Year: 2010

This study examined the adaptation accuracy of acrylic denture base processed using fluid-resin (PERform), injection-moldings (SRIvocap, Success, Mak Press), and two compression-molding techniques. The adaptation accuracy was measured primarily by the posterior border gaps at the mid-palatal area using a microscope and subsequently by weighing of the weight of the impression material between the denture base and master cast using hand-mixed and automixed silicone. The correlation between the data measured using these two test methods was examined. The PERform and Mak Press produced significantly smaller maximum palatal gap dimensions than the other groups (p<0.05). Mak Press also showed a significantly smaller weight of automixed silicone material than the other groups (p<0.05), while SR-Ivocap and Success showed similar adaptation accuracy to the compressionmolding denture. The correlationship between the magnitude of the posterior border gap and the weight of the silicone impression materials was affected by either the material or mixing variables.

Kim S.-Y.,Sungkyunkwan University | Kim S.-Y.,Konkuk University | Bae S.,Konkuk University | Choi K.-H.,Shinheung College | An S.,Konkuk University
Oncology Reports | Year: 2011

Akt is a well-established protein that regulates cell growth, survival and anti-apoptotic mechanisms. In this study, we demonstrated that hydrogen peroxide (H 2O 2)-induced oxidative stress regulates the activity of the anti-apoptotic protein Akt via the ubiquitin-proteasome degradation system. H 2O 2 induced cytotoxicity in PC12 cells and decreased the cellular protein and phosphorylation levels of Akt in concentration- and exposure time-dependent manner. This downregulation was blocked by the proteasome inhibitor MG132 and the Akt-specific inhibitor LY294002. In addition, an in vivo ubiquitination assay revealed that the degradation of Akt was mediated by the ubiquitin-mediated proteasome pathway and further demonstrated that this ubiquitination was dependent on the phosphorylation status of Akt. Furthermore, the exogenously overexpressed active form of Akt, but not its inactive form, induced resistance to H 2O 2-mediated cell death. These results suggested that H 2O 2-induced cytotoxicity was mediated by active Akt degradation.

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