Xiong K.-Z.,Guangdong Pharmaceutical University |
Xiong K.-Z.,Beijing Institute of Pharmacology and Toxicology |
Li Z.-G.,Shineway Pharmaceutical Group Ltd |
Zhang G.,Shineway Pharmaceutical Group Ltd |
And 13 more authors.
Chinese Journal of Pharmacology and Toxicology | Year: 2015
OBJECTIVE To study the effect of Shenmai injection (SMIJ) on immune function in rats by continuous iv administration by establishing a T cell-dependent antibody response (TDAR) model. METHODS SPF SD rats were ig given ciclosporin A (CSA) 0.02 g-kg-1 or iv given SMIJ solvent and SMIJ 0.6, 1.2 and 2.4 g-kg-1, once daily for 28 d. On the 15th and 22nd days, each rat except normal control group was sc given keyhole limpet hemocyanin (KLH) 2.4 mg-kg-1. The general state of the rats was observed throughout the trial while body mass and food consumption were measured weekly. The concentration of serum specific KLH-IgM antibody of rats was detected on the 20th day while the cencen- Tration of serum specific KLH-IgG antibody of rats was detected on the 29th day, when all rats were sacrificed , and the number of red blood cells, white blood cells and the classification of white blood cells were detected by automatic blood analyzer. The mass of the lung, liver, spleen and thymus was weighed by a scale, organ indexes were calculated, and the histopathological changes of the lung, liver, spleen, thymus and lymph nodes were observed by HE staining. RESULTS There was no death or significant difference in body mass and food consumption of rats between these groups during the experiment. The concentration of serum specific KLH-IgM and KLH-IgG antibody in model group was higher than in normal control group (P< 0.01), suggesting that KLH had caused immune response. When compared with the model group, the concentration of serum KLH-IgM and KLH-IgG antibody was significantly reduced in CSA group( P<0.01) while there was no significant change in SMIJ dose and SMIJ solvent groups. On the 29th day, the hematologic and organ index showed no difference and the organs had no lesions in the model group when compared with the normal control group. When compared with the model group, the number of white blood cells, eosinophils and lymphocytes and spleen and thymus organ indexes was significantly reduced in CSA group( P<0.05, P<0.01), the scope of splenic white pulp and the number of splenic lymphocytes were reduced and the boundary of lymphoid nodules was clear in CSA group, the results of hematology and organ indexes were not significantly changed and the organs had no obvious lesions in SMIJ and SMIJ solvent groups. CONCLUSION The TDAR model in SD rats is successfully established. Continuous iv injection of SMIJ for 28 d has no obvious effects on TDAR in SD rats.