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Wakayama-shi, Japan

Fatmawati N.W.D.,Okayama University of Science | Fatmawati N.W.D.,Udayana University | Sakaguchi Y.,University of Miyazaki | Suzuki T.,Okayama University of Science | And 6 more authors.
Acta Medica Okayama | Year: 2013

Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity; however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival. © 2013 by Okayama University Medical School.

Okamura H.,Osaka Prefecture University | Kayanoki M.,Shin Nakamura Chemical Co. | Takada K.,Shin Nakamura Chemical Co. | Nakajiri H.,Nakanuma Art Screen Co. | And 3 more authors.
Polymers for Advanced Technologies | Year: 2012

High-resolution screen printing was devised. New resist formulation contains a base polymer, which consists of acid-labile tetrahydropyranyl-protected carboxylic acid, hydroxyl, and methacrylic functions. As crosslinkers, multifunctional acrylates were employed. Photoacid generators were used for pattern formation. A 10-μm feature size of resist on a screen plate was obtained on irradiation at 365nm and followed by development on a stainless steel screen. Post-exposure curing improved the mechanical characteristics of the resist patterns. A 13-μm feature size silver circuit was successfully printed on poly(ethylene terephthalate) film without defect. © 2011 John Wiley & Sons, Ltd.

Okamura H.,Osaka Prefecture University | Matoba T.,Shin Nakamura Chemical Co. | Takada K.,Shin Nakamura Chemical Co. | Yamashita M.,Industrial Technology Center Of Okayama Prefecture | And 2 more authors.
Progress in Organic Coatings | Year: 2016

Photo-thermal dual curing system was devised and applied to screen printing technology. New formulation of acrylic anchor resins contains a base polymer, which consists of hydrophilic units and solvent-absorbing units. As crosslinkers, multifunctional acrylates were employed. The acrylic anchor resins coated on poly(ethylene terephthalate) films were effectively cured by simultaneous photo-irradiation and baking below 80. °C. Printing resolution was strongly affected by the formulation of the acrylic anchor resins.An 8-μm feature size silver circuit was successfully printed without defect on a poly(ethylene terephthalate) film having the acrylic anchor layer. © 2016 Elsevier B.V.

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