Suzuki T.,Shimadzu Corporation
Proteomics | Year: 2010
To establish a strategy for the comprehensive identification of human N-myristoylated proteins, the susceptibility of human cDNA clones to protein N-myristoylation was evaluated by metabolic labeling and MS analyses of proteins expressed in an insect cell-free protein synthesis system. One-hundred-and-forty-one cDNA clones with N-terminal Met-Gly motifs were selected as potential candidates from approximately 2000 Kazusa ORFeome project human cDNA clones, and their susceptibility to protein N-myristoylation was evaluated using fusion proteins, in which the N-terminal ten amino acid residues were fused to an epitope-tagged model protein. As a result, the products of 29 out of 141 cDNA clones were found to be effectively N-myristoylated. The metabolic labeling experiments both in an insect cell-free protein synthesis system and in the transfected COS-1 cells using full-length cDNA revealed that 27 out of 29 proteins were in fact N-myristoylated. Database searches with these 27 cDNA clones revealed that 18 out of 27 proteins are novel N-myristoylated proteins that have not been reported previously to be N-myristoylated, indicating that this strategy is useful for the comprehensive identification of human N-myristoylated proteins from human cDNA resources. Source
Shimadzu Corporation | Date: 2015-08-25
The invention relates to an ion generation device and an ion generation method, and more particularly to a device and a method which generates ions at the low pressure and then said ions can be transferred into the next stage in an off-axis manner. In the invention, ions from electrospray or other types of ion source are generated in the pressure which is lower than atmosphere pressure. A followed ion guide device can then transfer most of said generated ions into next stage in an off-axis manner, while most of neutral noise can be eliminated in this process.
Shimadzu Corporation | Date: 2015-09-08
Shimadzu Corporation | Date: 2015-06-16
A slide valve comprises: a casing having a pair of facing flow channels; a slide plate inserted into and removed from a space between the flow channels; and an annular sealing body slidingly moving in a facing direction of the flow channels inside the casing and coming into contact with the slide plate inserted between the flow channels to bring the slide valve into a closed state. A first sealing member sealing a gap between the sealing body and the inner peripheral wall surface is disposed on an inner peripheral surface of the annular sealing body and a second sealing member sealing a gap between the sealing body and the outer peripheral wall surface is formed on an outer peripheral surface of the annular sealing body.
Shimadzu Corporation | Date: 2015-05-22
A vacuum pump comprises a cylindrical rotor; a cylindrical stator which discharges gas in cooperation with the rotor; and a tubular base to which the stator is fixed. The stator has no fitting structure with respect to the base and is fixed to the base in a concentric state. A pin hole is formed on the stator and the base respectively, and a positioning pin for achieving the concentric state is inserted into each pin hole.