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Takamatsu, Japan

Seike T.,Kyushu University | Fujita K.,Kyushu University | Yamakawa Y.,Kyushu University | Kido M.A.,Kyushu University | And 4 more authors.
Clinical and Experimental Metastasis | Year: 2011

The incidence of brain metastasis is increasing, however, little is known about molecular mechanism responsible for lung cancer-derived brain metastasis and their development in the brain. In the present study, brain pathology was examined in an experimental model system of brain metastasis as well as in human brain with lung cancer metastasis. In an experimental model, after 3-6 weeks of intracardiac inoculation of human lung cancer-derived (HARA-B) cells in nude mice, wide range of brain metastases were observed. The brain sections showed significant increase in glial fibrillary acidic protein (GFAP)-positive astrocytes around metastatic lesions. To elucidate the role of astrocytes in lung cancer proliferation, the interaction between primary cultured mouse astrocytes and HARA-B cells was analyzed in vitro. Co-cultures and insert-cultures demonstrated that astrocytes were activated by tumor cell-oriented factors; macrophage migration inhibitory factor (MIF), interleukin-8 (IL-8) and plasminogen activator inhibitor-1 (PAI-1). Activated astrocytes produced interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1 β (IL-1β), which in turn promoted tumor cell proliferation. Semi-quantitative immunocytochemistry showed that increased expression of receptors for IL-6 and its subunits gp130 on HARA-B cells. Receptors for TNF-α and IL-1β were also detected on HARA-B cells but down-regulated after co-culture with astrocytes. Insert-culture with astrocytes also stimulated the proliferation of other lung cancer-derived cell lines (PC-9, QG56, and EBC-1). These results suggest that tumor cells and astrocytes stimulate each other and these mutual relationships may be important to understand how lung cancer cells metastasize and develop in the brain. © The Author(s) 2010.

Itagaki Y.,Ehime University | Watanabe S.,Ehime University | Yamaji T.,Shikoku Research Institute | Asamoto M.,Ehime University | And 2 more authors.
Journal of Power Sources | Year: 2012

Bi-layered cathodes with the LSM/LSM-YSZ structure for solid oxide fuel cells were successfully formed on the carbon-sputtered surface of a YSZ sheet by electrophoretic deposition (EPD). The thicknesses of the first layer of LSM-YSZ (LY) and the second layer of La 0.8Sr 0.2MnO 3 (LSM) could be controlled by adjusting the deposition time in the EPD process. The cathodic properties of the bi-layered structures were superior to those of the mono-layered structures, and were dependent on the thickness of each layer. Decreasing the thickness of the first layer and increasing that of the second layer tended to reduce both polarization and ohmic resistances. The optimal thickness of the first layer at the operating temperature of 600 °C was 4 μm, suggesting that an effective three-phase boundary was extended from the interface between the electrolyte and cathode film to around 4 μm thickness. © 2012 Elsevier B.V. All rights reserved.

Shikoku Research Institute | Date: 2010-04-28

A loose part monitoring method and system for preventing the generation of a false alarm as much as possible, including analyzing a group of detection signals that have been output from a plurality of sensors placed on a wall defining a fluid flow path, wherein the group of detection signals are analyzed for a rising gradient that is related to change in intensity of impulsive sound, a damping time that is related to change of intensity of the impulsive sound, and a frequency spectrum that is related to pitch of the impulsive sound in order to determine whether the group of detection signals are false or true.

Hiraga Y.,Shikoku Research Institute | Shigemoto N.,Shikoku Research Institute
Journal of Chemical Engineering of Japan | Year: 2012

The present study investigates the removal of As and Se oxoanions from solution by calcination products of Ca(OH) 2- Al(OH) 3 and CaCO 3-Al(OH) 3 mixtures with various Ca/(Ca+Al) molar ratios at 1273 K. In the calcination products obtained from the mixtures with Ca/(Ca+Al)=0.833, lime (CaO) and Ca-Al oxides were detected as the predominant and minor crystalline compounds, respectively. The calcination products were then immersed in solutions contained As and Se oxoanions to examine their removal e+ciency at rather high pH (>11). The highest arsenite removal e+ciencies were observed when the calcination product with Ca/(Ca+Al) molar ratio >0.7 was employed, while the arsenate removal ef- =ciency was independent of the Ca/(Ca+Al) ratio. The selenate removal e+ciency attained the peak value when the Ca/ (Ca+Al) ratio was 0.7-0.8. The maximum removal amount per unit weight of the calcination products for arsenite, arsenate, selenite, and selenate were 6-9, 4, 6-8 and 1.4-1.9 mmol/g, respectively, which were considerably higher than previously reported values. Upon reaction with the calcination product, arsenite, arsenate and selenite formed hard-soluble Ca salts, Ca(HAsO 3), Ca 3(AsO 4) 2 with Ca5(AsO4)3(OH) and CaSeO3, respectively, where the quick lime (CaO) in the calcination product is an active component for removing these oxoanions. On the other hand, selenate was removed by forming ettringite substituted by selenate, Ca 6Al 2(SeO 4) 3(OH) 12·26H 2O, where the synergistic effect of Ca and Al compounds in the calcination product should be exhibited. © 2012 The Society of Chemical Engineers, Japan.

Hiraga Y.,Shikoku Research Institute | Shigemoto N.,Shikoku Research Institute
Journal of Chemical Engineering of Japan | Year: 2012

Ca(OH) 2-Al(OH) 3 and CaCO 3-Al(OH) 3 mixtures with Ca/(Ca+Al) ratios in the range 0 to 1 were calcined in the temperature range 873-1673 K for studying the efiect of the calcination temperature and Ca/(Ca+Al) ratio on the calcinations products. XRD analysis showed that the mixtures with Ca/(Ca+Al) ratios in the range 0.68-0.83 and calcined around 1273 K gave lime (CaO) as the major product and mayenite (Ca 12Al 14O 33), Ca 9A l6O 18, and Ca 5A l6O 14 as the minor products. These calcination products indicated that the eficiencies of the removal of chromate and borate from the solution were high. Upon immersing these calcination products in chromate and borate solutions, Ca 4A l2CrO 10·12H 2O and Ca 4A l2(HBO 3) 0.5(OH) 13·6H 2O were formed, respectively; the Ca/(Ca+Al) molar ratio in these compounds was 0.68, which was close to that in the raw mixtures. A Ca/(CafiAl) ratio in the range 0.68-0.83 resulted in the optimum removal of chromate and borate from the solution. The Ca/(Ca+Al) ratio was more efiective than these crystalline components in these calcination products. Further, Ca(B(OH) 4) 2·2H 2O precipitated as a result of borate removal. © 2012 The Society of Chemical Engineers, Japan.

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