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Niu N.,CAS Institute of Botany | Niu N.,Shijiazhuang Academy of Agriculture and Forestry science | Wu B.,CAS Institute of Botany | Yang P.,CAS Wuhan Botanical Garden | And 2 more authors.
Scientia Horticulturae | Year: 2013

The aim of this study was to deepen our understanding of the proteomic profiles related to skin coloration at the whole proteome level through the analysis of multiple protein spots. A comparative proteomics analysis was conducted on the berry skins of red and white progeny derived from a single cross, 'Jingxiu' (red). ×. 'Muscat of Alexandria' (white), to minimize genetic background differences. Among the 1000-1800 spots assessed, the levels of 57, 70 and 26 proteins were lower in the skins of white progeny, compared to the skins of red progeny at about two weeks before veraison, at veraison and at maturity, respectively. Alternatively, the levels of 54, 50 and 23 proteins were higher at about two weeks before veraison, at veraison and at maturity, respectively. The levels of proteins involved in anthocyanin biosynthesis, especially UFGT and AOMT, were lower in the skins of white progeny at veraison and at maturity, and the levels of most proteins involved in amino acid metabolism were found to be lower before veraison. Almost all differentially accumulated proteins involved in energy production were found before and at veraison, while there was no difference detected in the levels of glycolysis, tricarboxylic acid cycle and photosynthesis between the skins of red and white progeny. The differential accumulation of enzyme proteins involved in anthocyanin synthesis had a decisive influence on the different skin colorization of red and white progeny. Many proteins related to metabolism and energy production were also differentially accumulated. In addition, many differences in the skin proteomes of white and red progeny were apparent before veraison, i.e. before skins developed coloration. © 2013 Elsevier B.V.

Tao S.,China Agricultural University | Wang W.,Shijiazhuang Academy of Agriculture and Forestry science | Chen F.,Henan University of Technology | Zhang H.,China Agricultural University | Xue W.,China Agricultural University
Journal of the Chinese Cereals and Oils Association | Year: 2015

A New method in the determination of peroxide value has been developed, namely by differential pulse anodic stripping voltammetry; adopted chitosan modified screen printing electrode scan vegetable oil samples to instead of titration to determine changes of the iodine ion concentration. In the research described in the paper, the proposed method determine peroxide value of 40 saled vegetable oil samples, and compare with the national standard method (GB/T 5538-2005). Through evaluate comprehensive deviation degree and En value, it was determined that the results were all satisfied in the qualitative evaluation, and had 37 satisfied ones in respect of quantitative judgment, the overall satisfaction rate was 92.5%. Especially in the range of 0.016~0.075 mmol/kg the result is more accurate and satisfaction rate can reach 91.6%. This research showed that compared with the national standard method, the new method in operational, security, etc. has a certain advantage, and the result accuracy could be recognized within the limits of certain peroxide value range. ©, 2014, Editorial Department, Chinese Cereals and Oils Association. All right reserved.

Ma J.,University of Western Australia | Ma J.,CSIRO | Du G.,Agricultural University of Hebei | Li X.,Agricultural University of Hebei | And 2 more authors.
Molecular Genetics and Genomics | Year: 2015

Malondialdehyde (MDA) is a naturally occurring product of lipid peroxidation and the level of MDA in plant is often used as a parameter to evaluate the damage to plants’ cells due to stress. Plant with lower amounts of MDA under drought conditions is generally considered as more tolerant to drought. In this study, a population of recombinant inbred lines was used to map the quantitative trait locus (QTLs) that controlled MDA content under well-watered condition (WW) and water deficit (WD) condition. A major QTL, designated as Qheb.mda-3B, was detected on the long arm of chromosome 3B. Based on interval mapping analysis, Qheb.mda-3B explained 31.5 and 39.0 % of the phenotypic variance under WW and WD conditions, respectively. Qheb.mda-3B was located in the same interval as a previously identified QTL (Qcrs.cpi-3B) that controlled resistance to Fusarium crown rot (FCR), a fungal disease caused by Fusarium species. Three pairs of near-isogenic lines (NILs) previously developed for Qcrs.cpi-3B were found to show significant differences in MDA content under WD condition. These results suggested that same set of genes is likely to be involved in drought tolerance and FCR resistance in wheat. © 2015, Springer-Verlag Berlin Heidelberg.

Wang M.,Shandong University | Liu C.,Shandong University | Xing T.,Shandong University | Wang Y.,Shijiazhuang Academy of Agriculture and Forestry science | Xia G.,Shandong University
BMC Genomics | Year: 2015

Background: Allopolyploid genome needs wide structural variation to deal with genomic shock. The introgression line, generated via asymmetric somatic hybridization, is introgressed with a minimum of exogenous chromatin, which also leads to genomic shock to induce genetic variation. However, the extent of its genomic variation and its difference from allopolyploidies remains unknown. Methods: Here, we explored this issue using the bread wheat cultivar SR3, a derivative of an asymmetric somatic hybrid between the cultivar JN177 and an accession of tall wheatgrass (Thinopyrum elongatum). The ESTs (expressed sequence taqs) were large-scale sequenced using the cDNA library constructed in each of SR3 and JN177. Point mutations and indels (insertions and deletions) of SR3 were calculated, and their difference from the genetic variation of bread wheat and its ancestors were compared, with aim to analyze the extent and pattern of sequence variation induced by somatic hybridization. Results: Both point mutations and indels (insertions and deletions) were frequently induced by somatic hybridization in the coding sequences. While the genomic shock caused by allopolyploidization tends to favor deletion over insertion, there was no evidence for such a preference following asymmetric somatic hybridization. The GC content of sequence adjacent to indel sites was also distinct from what has been observed in allopolyploids. Conclusions: This study demonstrates that asymmetric somatic hybridization induces high frequency of genetic variation in a manner partially different from allopolipoidization. Asymmetric somatic hybridization provides appropriate material to comprehensively explore the nature of the genetic variation induced by genomic shock. © 2015 Wang et al.

Dong Z.,CAS Institute of Genetics and Developmental Biology | Dong Z.,University of Chinese Academy of Sciences | Dong Z.,Shijiazhuang Academy of Agriculture and Forestry science | Shi L.,CAS Institute of Genetics and Developmental Biology | And 7 more authors.
International Journal of Molecular Sciences | Year: 2013

Both symbiosis between legumes and rhizobia and nitrogen fixation in functional nodules are dramatically affected by salt stress. Better understanding of the molecular mechanisms that regulate the salt tolerance of functional nodules is essential for genetic improvement of nitrogen fixation efficiency. microRNAs (miRNAs) have been implicated in stress responses in many plants and in symbiotic nitrogen fixation (SNF) in soybean. However, the dynamic regulation of miRNAs in functioning nodules during salt stress response remains unknown. We performed deep sequencing of miRNAs to understand the miRNA expression profile in normal or salt stressed-soybean mature nodules. We identified 110 known miRNAs belonging to 61 miRNA families and 128 novel miRNAs belonging to 64 miRNA families. Among them, 104 miRNAs were dramatically differentially expressed (>2-fold or detected only in one library) during salt stress. qRT-PCR analysis of eight miRNAs confirmed that these miRNAs were dynamically regulated in response to salt stress in functional soybean nodules. These data significantly increase the number of miRNAs known to be expressed in soybean nodules, and revealed for the first time a dynamic regulation of miRNAs during salt stress in functional nodules. The findings suggest great potential for miRNAs in functional soybean nodules during salt stress. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

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