Hao J.,Hebei University of Science and Technology |
Wu T.,Hebei University of Science and Technology |
Li H.,Hebei University of Science and Technology |
Wang W.,Shijiazhuang Academy of Agricultural and Forestry Science |
Liu H.,China Agricultural University
Food Chemistry | Year: 2016
In the present study, the dual effects of slightly acidic electrolyzed water (SAEW) treatment on γ-aminobutyric acid (GABA) and rutin accumulation of germinated buckwheat were evaluated during germination. The results showed that SAEW treatment (pH 5.83, ACC of 20.3 mg/L) could promote the accumulation of GABA and rutin in germinated buckwheat. The GABA and rutin contents of SAEW-germinated buckwheat reached 143.20 and 739.9 mg/100 g respectively, which is significantly higher than those of control (P < 0.05). Moreover, SAEW treatment could increase the activity of glutamic acid decarboxylase (GAD) and phenylalanine ammonialyase (PAL) and thus result in the GABA and rutin accumulation of germinated buckwheat. The results suggested that SAEW treatment could promote the rutin accumulation of germinated buckwheat by influencing phenylpropanoid secondary metabolic pathway instead of the inhibition of rutin degrading enzyme (RDE) activity. In addition, SAEW treatment had no adverse impact on the sprouts growth and could reduce the microbial populations of germinated buckwheat during germination. © 2016 Elsevier Ltd. All rights reserved.
PubMed | Hebei University of Science and Technology, Shijiazhuang Academy of Agricultural and Forestry Science and China Agricultural University
Type: | Journal: Food chemistry | Year: 2016
In the present study, the dual effects of slightly acidic electrolyzed water (SAEW) treatment on -aminobutyric acid (GABA) and rutin accumulation of germinated buckwheat were evaluated during germination. The results showed that SAEW treatment (pH 5.83, ACC of 20.3 mg/L) could promote the accumulation of GABA and rutin in germinated buckwheat. The GABA and rutin contents of SAEW-germinated buckwheat reached 143.20 and 739.9 mg/100 g respectively, which is significantly higher than those of control (P<0.05). Moreover, SAEW treatment could increase the activity of glutamic acid decarboxylase (GAD) and phenylalanine ammonialyase (PAL) and thus result in the GABA and rutin accumulation of germinated buckwheat. The results suggested that SAEW treatment could promote the rutin accumulation of germinated buckwheat by influencing phenylpropanoid secondary metabolic pathway instead of the inhibition of rutin degrading enzyme (RDE) activity. In addition, SAEW treatment had no adverse impact on the sprouts growth and could reduce the microbial populations of germinated buckwheat during germination.
Xu H.,CAS Institute of Genetics and Developmental Biology |
Yi Y.,Henan University of Technology |
Ma P.,CAS Institute of Genetics and Developmental Biology |
Qie Y.,CAS Institute of Genetics and Developmental Biology |
And 4 more authors.
Theoretical and Applied Genetics | Year: 2015
Key message: A new broad-spectrum powdery mildew resistanceallelePm2cwas identified and mapped in Chinese wheat landrace Niaomai. Abstract: Chinese wheat landrace Niaomai showed resistance to 27 of 28 Chinese Blumeria graminis f. sp tritici (Bgt) races. Genetic analysis of an F2 population and its derived F2:3 families from the cross Niaomai × Mingxian 169 and backcross population, Niaomai/2*Mingxian 169, indicated that the resistance of Niaomai to Bgt races was conferred by a single dominant resistance gene, temporarily designated PmNM. Molecular tagging showed that PmNM was located on chromosome 5DS and flanked by SSR markers Xcfd81 and Xcfd78 with the genetic distances of 0.1/0.4 cM and 4.9/7.5 cM, respectively. Niaomai showed a different array of responses compared to lines with Pm2a, Pm2b, PmD57-5D, PmLX66, PmX3986-2 and Pm48 genes, sharing the same Xcfd81 allele but differing from Xcfd78 allele for Pm2a and Pm2b lines. Allelism tests based on crosses of Niaomai with Ulka/8*Cc and KM2939 showed that PmNM is allelic to Pm2a and Pm2b. We concluded that PmNM is a new allele of Pm2, re-designated Pm2c. Pm2c could be transferred into wheat cultivars by marker-assisted selection to improve the powdery mildew resistance of breeding cultivars/lines. © 2015, Springer-Verlag Berlin Heidelberg.
Ma J.,University of Western Australia |
Ma J.,CSIRO |
du G.,Agricultural University of Hebei |
Li X.,Agricultural University of Hebei |
And 3 more authors.
Plant Molecular Biology Reporter | Year: 2015
At an early stage of crop development, the rate of growth is largely determined by leaf characteristics. Plants with rapid leaf area development could save more water for transpiration and crop growth. In our study, a recombinant inbred family was used to identify quantitative trait loci (QTL) controlling leaf length (LL), leaf width (LW), and leaf area (LA) in wheat seedlings under well-watered (WW) and PEG-induced water-deficit (WD) conditions. A total of five QTL for LW, LL, and LA were detected, most of which were reported for the first time. A “constitutive” QTL for LW (Qheb.LW-3B), located on the long arm of chromosome 3B, was consistently detected under two water conditions, explaining 17.7 % of the phenotypic variance with a LOD value of 7.20 under WW condition and 13.3 % of the phenotypic variance with a LOD value of 4.87 under WD condition. The other four “adaptive” QTL were detected under a single water condition only. These QTL include the following: Qheb.LW-5B for LW (WW condition), Qheb.LL-3A, and Qheb.LL-5B for LL (WD condition) and Qheb.LA-3B for LA (WW condition). Four pairs of near isogenic lines (NILs) were developed to validate the effects of Qheb.LW-3B. The allele from the parent “CSCR6” increased the LW by an average of 8.2 % under WW condition and 13.8 % under WD condition, respectively. The position and effects of Qheb.LW-3B was confirmed. Qheb.LW-3B would be a valuable genetic resource to improve wheat seedling early establishment. The NILs we have generated would be useful for further characterization of Qheb.LW-3B, in studying its interaction with other traits of agronomic importance and in developing markers that can be reliably used to follow this major locus. © 2015 Springer Science+Business Media New York
He G.-H.,Chinese Academy of Agricultural Sciences |
Xu J.-Y.,Chinese Academy of Agricultural Sciences |
Wang Y.-X.,Shijiazhuang Academy of Agricultural and Forestry science |
Liu J.-M.,Chinese Academy of Agricultural Sciences |
And 4 more authors.
BMC Plant Biology | Year: 2016
Background: Drought stress is one of the major causes of crop loss. WRKY transcription factors, as one of the largest transcription factor families, play important roles in regulation of many plant processes, including drought stress response. However, far less information is available on drought-responsive WRKY genes in wheat (Triticum aestivum L.), one of the three staple food crops. Results: Forty eight putative drought-induced WRKY genes were identified from a comparison between de novo transcriptome sequencing data of wheat without or with drought treatment. TaWRKY1 and TaWRKY33 from WRKY Groups III and II, respectively, were selected for further investigation. Subcellular localization assays revealed that TaWRKY1 and TaWRKY33 were localized in the nuclei in wheat mesophyll protoplasts. Various abiotic stress-related cis-acting elements were observed in the promoters of TaWRKY1 and TaWRKY33. Quantitative real-time PCR (qRT-PCR) analysis showed that TaWRKY1 was slightly up-regulated by high-temperature and abscisic acid (ABA), and down-regulated by low-temperature. TaWRKY33 was involved in high responses to high-temperature, low-temperature, ABA and jasmonic acid methylester (MeJA). Overexpression of TaWRKY1 and TaWRKY33 activated several stress-related downstream genes, increased germination rates, and promoted root growth in Arabidopsis under various stresses. TaWRKY33 transgenic Arabidopsis lines showed lower rates of water loss than TaWRKY1 transgenic Arabidopsis lines and wild type plants during dehydration. Most importantly, TaWRKY33 transgenic lines exhibited enhanced tolerance to heat stress. Conclusions: The functional roles highlight the importance of WRKYs in stress response. © 2016 He et al.
Liu R.,Chinese Academy of Sciences |
Zhang Y.,Chinese Academy of Sciences |
Zhang B.,Chinese Academy of Sciences |
Ban J.,Shijiazhuang Academy of Agricultural and Forestry science |
Wei Y.,Chinese Academy of Sciences
Cereal Chemistry | Year: 2016
The effects of vacuum mixing on the structural characteristics and physical properties of noodle dough were investigated using three leading Chinese wheat cultivars. Texture profile analysis showed that vacuum mixed doughs when sheeted all gave significantly higher levels of adhesiveness, elasticity, and chewiness than doughs from nonvacuum mixing. The cross section of sheeted dough mixed at 0.06 MPa had a more continuous and compact microstructure with fewer holes and gaps, as well as more even protein distribution at the surface, as evidenced by scanning electron microscopy and Fourier transform infrared microimaging. However, a higher degree of vacuum was detrimental to the developed network for weak dough. Dough mixed at 0.06 MPa had higher glutenin macropolymer content and lower free thiol group concentration compared with nonvacuum mixed doughs, which may largely relate to the improvement of dough texture. The development of the gluten network for weak gluten flour was more sensitive to the degree of vacuum. © 2016 AACC International, Inc.
Li G.,Henan Institute of Science and Technology |
Liu B.,Henan Institute of Science and Technology |
Zhang G.,Shijiazhuang Academy of Agricultural and Forestry science |
Zeng J.,Henan Institute of Science and Technology |
And 2 more authors.
Tropical Journal of Pharmaceutical Research | Year: 2014
Results: FTIR spectrum showed new absorption peaks at 1033 cm-1 indicating that an esterification cross-linking reaction was found between sweet potato starch and sodium trimetaphosphate. Similar gelatinization temperature (70°C), enthalpy change (10 J/g), and peak viscosity (600 cp) were obtained for sweet potato starch phosphodiester and the raw starch indicating that their gelatinization properties were identical. Compared with sweet potato starch, digestible starch content of sweet potato starch phosphodiester decreased sharply (from 63.4 to 15.8%), while digestion resistance starch content increased significantly (from 14.5 to 58.7%). Based on completion of starch hydrolysis, the glycaemic index (GI) of sweet potato starch phosphodiester was predicted to be 66.31.Conclusion: Derived sweet potato starch phosphodiester presents higher digestibility and may be useful as a medium glycemic index (GI) food for diabetic patients.Purpose: To analyze the physicochemical properties and in vitro digestibility of sweet potato starch phosphodiester prepared using sodium trimetaphosphate.Methods: The physicochemical properties of sweet potato starch phosphodiester were analyzed by using infrared spectrometry (IR), differential scanning calorimetry (DSC) and rapid visco-analyser (RVA). In addition, an in vitro digestibility method was applied to investigate starch digestion performances. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.
Li G.-L.,Henan Institute of Science and Technology |
Zhang G.-C.,Shijiazhuang Academy of Agricultural and Forestry science |
Liu B.-G.,Henan Institute of Science and Technology |
Li F.,Henan Institute of Science and Technology |
And 2 more authors.
Modern Food Science and Technology | Year: 2014
The influences of steam exploded pressure, retained time, and sample moisture content on starch molecular structure of indica rice starch treated with steam explosion were analyzed by gel chromatography (GPC), ultraviolet-visible spectroscopy (UV), and Fourier transform infrared spectroscopy (FT-IR). The results showed that, with the increase of steam exploded pressure, retained time, and sample moisture content, molecular weight of indica rice starch were decreased, and the proportion of starch short chain pieces was increased. In addition, the intermediate absorption peak gradually disappeared, and the degree of polymerization of indica rice starch chains was decreased. The maximum absorption wavelength of UV spectrum showed a blue shift. As the intensity of steam explosion treatment increased, the intensity of aldehyde group absorption peak at 1650 cm-1 in the infrared absorption spectrogram increased, and starch infrared crystallization index (ICI) increased. Steam explosion technology effectively degraded the polymerization degree of indica rice starch chains and increased the crystallinity starch granule.
Wang J.,Chinese Academy of Sciences |
Sun J.,Chinese Academy of Sciences |
Sun J.,CAS Institute of Biophysics |
Miao J.,Chinese Academy of Sciences |
And 9 more authors.
Annals of Botany | Year: 2013
Background and Aims Phosphorus deficiency is a major limiting factor for crop yield worldwide. Previous studies revealed that PHR1 and it homologues play a key role in regulating the phosphate starvation response in plants. However, the function of PHR homologues in common wheat (Triticum aestivum) is still not fully understood. The aim of the study was to characterize the function of PHR1 genes in regulating phosphate signalling and plant growth in wheat. Methods Wheat transgenic lines over-expressing a wheat PHR1 gene were generated and evaluated under phosphorus-deficient and-sufficient conditions in hydroponic culture, a soil pot trial and two field experiments. Key Results Three PHR1 homologous genes Ta-PHR1-A1, B1 and D1 were isolated from wheat, and the function of Ta-PHR1-A1 was analysed. The results showed that Ta-PHR1-A1 transcriptionally activated the expression of Ta-PHT1.2 in yeast cells. Over-expressing Ta-PHR1-A1 in wheat upregulated a subset of phosphate starvation response genes, stimulated lateral branching and improved phosphorus uptake when the plants were grown in soil and in nutrient solution. The data from two field trials demonstrated that over-expressing Ta-PHR1-A1 increased grain yield by increasing grain number per spike. Conclusions TaPHR1 is involved in phosphate signalling in wheat, and was valuable in molecular breeding of crops, with improved phosphorus use efficiency and yield performance. © 2013 The Author. Published by Oxford University Press on behalf of the Annals of Botany Company.
Ma P.,CAS Institute of Genetics and Developmental Biology |
Xu H.,CAS Institute of Genetics and Developmental Biology |
Li L.,Chinese Academy of Agricultural Sciences |
Zhang H.,CAS Institute of Genetics and Developmental Biology |
And 5 more authors.
Frontiers in Plant Science | Year: 2016
Powdery mildew has a negative impact on wheat production. Novel host resistance increases the diversity of resistance genes and helps to control the disease. In this study, wheat line FG-1 imported from France showed a high level of powdery mildew resistance at both the seedling and adult stages. An F2 population and F2:3 families from the cross FG-1 × Mingxian 169 both fit Mendelian ratios for a single dominant resistance gene when tested against multiple avirulent Blumeria tritici f. sp. tritici (Bgt) races. This gene was temporarily designated PmFG. PmFG was mapped on the multi-allelic Pm2 locus of chromosome 5DS using seven SSR, 10 single nucleotide polymorphism (SNP)-derived and two SCAR markers with the flanking markers Xbwm21/Xcfd81/Xscar112 (distal) and Xbwm25 (proximal) at 0.3 and 0.5 cM being the closest. Marker SCAR203 co-segregated with PmFG. Allelism tests between PmFG and documented Pm2 alleles confirmed that PmFG was allelic with Pm2. Line FG-1 produced a significantly different reaction pattern compared to other lines with genes at or near Pm2 when tested against 49 Bgt isolates. The PmFG-linked marker alleles detected by the SNP-derived markers revealed significant variation between FG-1 and other lines with genes at or near Pm2. It was concluded that PmFG is a new allele at the Pm2 locus. Data from seven closely linked markers tested on 31 wheat cultivars indicated opportunities for marker-assisted pyramiding of this gene with other genes for powdery mildew resistance and additional traits. © 2016 Ma, Xu, Li, Zhang, Han, Xu, Fu, Zhang and An.