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Hao J.,Hebei University of Science and Technology | Wu T.,Hebei University of Science and Technology | Li H.,Hebei University of Science and Technology | Wang W.,Shijiazhuang Academy of Agricultural and Forestry Science | Liu H.,China Agricultural University
Food Chemistry | Year: 2016

In the present study, the dual effects of slightly acidic electrolyzed water (SAEW) treatment on γ-aminobutyric acid (GABA) and rutin accumulation of germinated buckwheat were evaluated during germination. The results showed that SAEW treatment (pH 5.83, ACC of 20.3 mg/L) could promote the accumulation of GABA and rutin in germinated buckwheat. The GABA and rutin contents of SAEW-germinated buckwheat reached 143.20 and 739.9 mg/100 g respectively, which is significantly higher than those of control (P < 0.05). Moreover, SAEW treatment could increase the activity of glutamic acid decarboxylase (GAD) and phenylalanine ammonialyase (PAL) and thus result in the GABA and rutin accumulation of germinated buckwheat. The results suggested that SAEW treatment could promote the rutin accumulation of germinated buckwheat by influencing phenylpropanoid secondary metabolic pathway instead of the inhibition of rutin degrading enzyme (RDE) activity. In addition, SAEW treatment had no adverse impact on the sprouts growth and could reduce the microbial populations of germinated buckwheat during germination. © 2016 Elsevier Ltd. All rights reserved.

Ma J.,University of Western Australia | Ma J.,CSIRO | du G.,Agricultural University of Hebei | Li X.,Agricultural University of Hebei | And 3 more authors.
Plant Molecular Biology Reporter | Year: 2015

At an early stage of crop development, the rate of growth is largely determined by leaf characteristics. Plants with rapid leaf area development could save more water for transpiration and crop growth. In our study, a recombinant inbred family was used to identify quantitative trait loci (QTL) controlling leaf length (LL), leaf width (LW), and leaf area (LA) in wheat seedlings under well-watered (WW) and PEG-induced water-deficit (WD) conditions. A total of five QTL for LW, LL, and LA were detected, most of which were reported for the first time. A “constitutive” QTL for LW (Qheb.LW-3B), located on the long arm of chromosome 3B, was consistently detected under two water conditions, explaining 17.7 % of the phenotypic variance with a LOD value of 7.20 under WW condition and 13.3 % of the phenotypic variance with a LOD value of 4.87 under WD condition. The other four “adaptive” QTL were detected under a single water condition only. These QTL include the following: Qheb.LW-5B for LW (WW condition), Qheb.LL-3A, and Qheb.LL-5B for LL (WD condition) and Qheb.LA-3B for LA (WW condition). Four pairs of near isogenic lines (NILs) were developed to validate the effects of Qheb.LW-3B. The allele from the parent “CSCR6” increased the LW by an average of 8.2 % under WW condition and 13.8 % under WD condition, respectively. The position and effects of Qheb.LW-3B was confirmed. Qheb.LW-3B would be a valuable genetic resource to improve wheat seedling early establishment. The NILs we have generated would be useful for further characterization of Qheb.LW-3B, in studying its interaction with other traits of agronomic importance and in developing markers that can be reliably used to follow this major locus. © 2015 Springer Science+Business Media New York

Xu H.,CAS Institute of Genetics and Developmental Biology | Yi Y.,Henan University of Technology | Ma P.,CAS Institute of Genetics and Developmental Biology | Qie Y.,CAS Institute of Genetics and Developmental Biology | And 4 more authors.
Theoretical and Applied Genetics | Year: 2015

Key message: A new broad-spectrum powdery mildew resistanceallelePm2cwas identified and mapped in Chinese wheat landrace Niaomai. Abstract: Chinese wheat landrace Niaomai showed resistance to 27 of 28 Chinese Blumeria graminis f. sp tritici (Bgt) races. Genetic analysis of an F2 population and its derived F2:3 families from the cross Niaomai × Mingxian 169 and backcross population, Niaomai/2*Mingxian 169, indicated that the resistance of Niaomai to Bgt races was conferred by a single dominant resistance gene, temporarily designated PmNM. Molecular tagging showed that PmNM was located on chromosome 5DS and flanked by SSR markers Xcfd81 and Xcfd78 with the genetic distances of 0.1/0.4 cM and 4.9/7.5 cM, respectively. Niaomai showed a different array of responses compared to lines with Pm2a, Pm2b, PmD57-5D, PmLX66, PmX3986-2 and Pm48 genes, sharing the same Xcfd81 allele but differing from Xcfd78 allele for Pm2a and Pm2b lines. Allelism tests based on crosses of Niaomai with Ulka/8*Cc and KM2939 showed that PmNM is allelic to Pm2a and Pm2b. We concluded that PmNM is a new allele of Pm2, re-designated Pm2c. Pm2c could be transferred into wheat cultivars by marker-assisted selection to improve the powdery mildew resistance of breeding cultivars/lines. © 2015, Springer-Verlag Berlin Heidelberg.

Li G.-L.,Henan Institute of Science and Technology | Zhang G.-C.,Shijiazhuang Academy of Agricultural and Forestry Science | Liu B.-G.,Henan Institute of Science and Technology | Li F.,Henan Institute of Science and Technology | And 2 more authors.
Modern Food Science and Technology | Year: 2014

The influences of steam exploded pressure, retained time, and sample moisture content on starch molecular structure of indica rice starch treated with steam explosion were analyzed by gel chromatography (GPC), ultraviolet-visible spectroscopy (UV), and Fourier transform infrared spectroscopy (FT-IR). The results showed that, with the increase of steam exploded pressure, retained time, and sample moisture content, molecular weight of indica rice starch were decreased, and the proportion of starch short chain pieces was increased. In addition, the intermediate absorption peak gradually disappeared, and the degree of polymerization of indica rice starch chains was decreased. The maximum absorption wavelength of UV spectrum showed a blue shift. As the intensity of steam explosion treatment increased, the intensity of aldehyde group absorption peak at 1650 cm-1 in the infrared absorption spectrogram increased, and starch infrared crystallization index (ICI) increased. Steam explosion technology effectively degraded the polymerization degree of indica rice starch chains and increased the crystallinity starch granule.

He G.-H.,Chinese Academy of Agricultural Sciences | Xu J.-Y.,Chinese Academy of Agricultural Sciences | Wang Y.-X.,Shijiazhuang Academy of Agricultural and Forestry Science | Liu J.-M.,Chinese Academy of Agricultural Sciences | And 4 more authors.
BMC Plant Biology | Year: 2016

Background: Drought stress is one of the major causes of crop loss. WRKY transcription factors, as one of the largest transcription factor families, play important roles in regulation of many plant processes, including drought stress response. However, far less information is available on drought-responsive WRKY genes in wheat (Triticum aestivum L.), one of the three staple food crops. Results: Forty eight putative drought-induced WRKY genes were identified from a comparison between de novo transcriptome sequencing data of wheat without or with drought treatment. TaWRKY1 and TaWRKY33 from WRKY Groups III and II, respectively, were selected for further investigation. Subcellular localization assays revealed that TaWRKY1 and TaWRKY33 were localized in the nuclei in wheat mesophyll protoplasts. Various abiotic stress-related cis-acting elements were observed in the promoters of TaWRKY1 and TaWRKY33. Quantitative real-time PCR (qRT-PCR) analysis showed that TaWRKY1 was slightly up-regulated by high-temperature and abscisic acid (ABA), and down-regulated by low-temperature. TaWRKY33 was involved in high responses to high-temperature, low-temperature, ABA and jasmonic acid methylester (MeJA). Overexpression of TaWRKY1 and TaWRKY33 activated several stress-related downstream genes, increased germination rates, and promoted root growth in Arabidopsis under various stresses. TaWRKY33 transgenic Arabidopsis lines showed lower rates of water loss than TaWRKY1 transgenic Arabidopsis lines and wild type plants during dehydration. Most importantly, TaWRKY33 transgenic lines exhibited enhanced tolerance to heat stress. Conclusions: The functional roles highlight the importance of WRKYs in stress response. © 2016 He et al.

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