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Zhiyuan N.,Nanjing Agricultural University | Zhao R.,Nanjing Agricultural University | Chen H.,Xinjiang Academy of Agricultural and Reclamation science | Ai N.,Shihezi Agricultural Science and Tec Research Center | And 6 more authors.
Crop Science | Year: 2013

Verticillium wilt (VW) is one of the important damaging cotton (Gossypium hirsutum L.) diseases worldwide. The use of resistant cultivars has long been considered as the most practical and effective means of controlling VW. The upland cotton cultivar Prema has high VW resistance and is useful for breeding new cultivars resistant to VW. To tag the resistance quantitative trait loci (QTL) in this cultivar, a population of 180 recombinant inbred lines was developed via single seed decent from a cross between the resistant Prema and the susceptible cultivar 86-1. Verticillium wilt resistance trials were conducted in the artificial disease nursery inoculated with mixed isolates and greenhouse individually with VW defoliating isolates V991 and VD8 over a 2-yr period. Based on composite interval mapping analysis of the 2010 and 2011 data sets of adjusted disease reaction in the artificial disease nursery, seven resistant QTL were detected. Among them, one major VW-resistant QTL, which explained 62.83% of the phenotypic variation on the average with logarithm of the odds scores ranging from 23.37 to 26.73, was detected consistently and anchored on chromosome (chr.) D9. Greenhouse experiments revealed the existence of five significant QTL on chr. A9, D3, D11, and D9. Our results showed that qVW-D9-1 is a major broad-spectrum VW resistance QTL. Identification of this QTL will facilitate the marker-assisted selection of VW resistance in cotton breeding. © Crop Science Society of America.

Wang Y.,Nanjing Agricultural University | Ning Z.,Nanjing Agricultural University | Hu Y.,Nanjing Agricultural University | Chen J.,Nanjing Agricultural University | And 5 more authors.
PLoS ONE | Year: 2015

Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation sequencing (NGS) platform has greatly expedited SNP discovery in crops with highly repetitive and complex genomes. Here we applied restriction-site associated DNA (RAD) sequencing technology for de novo SNP discovery in allotetraploid cotton. We identified 21,109 SNPs between the two parents and used these for genotyping of 161 recombinant inbred lines (RILs). Finally, a high dense linkage map comprising 4,153 loci over 3500-cM was developed based on the previous result. Using this map quantitative trait locus (QTLs) conferring fiber strength and Verticillium Wilt (VW) resistance were mapped to a more accurate region in comparison to the 1576-cM interval determined using the simple sequence repeat (SSR) genetic map. This suggests that the newly constructed map has more power and resolution than the previous SSR map. It will pave the way for the rapid identification of the marker-assisted selection in cotton breeding and cloning of QTL of interest traits. Copyright: © 2015 Wang et al.

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