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Allahābād, India

Kuddus M.,Integral University | Kuddus M.,Shaqra University | Ramteke P.W.,SHIATS
Critical Reviews in Microbiology

Microbial proteases that occupy a pivotal position with respect to their commercial applications are most important hydrolytic enzymes and have been studied extensively since the advent of enzymology. Cold-adapted microorganisms are potential source of cold-active proteases and they have been isolated from the cold regions. Although there are many microbial sources available for producing proteases, only few are recognized as commercial producer. Cold-active proteases along with their producing microbes are of commercial value and find multiple applications in various industrial and biotechnological sectors such as additives in detergents, additives in food industries, environmental bioremediations, biotransformation and molecular biology applications. Therefore, cold-active proteases are the enzymes of choice for many biotechnologists, microbiologists, biochemists, environmentalists and biochemical engineers. In the present review, we discuss some novel sources along with recent developments in production and biotechnological applications of cold-active microbial proteases. © 2012 Informa Healthcare USA, Inc. Source

Kuddus M.,Hail University | Joseph B.,Shaqra University | Wasudev Ramteke P.,SHIATS
Biocatalysis and Agricultural Biotechnology

A novel laccase enzyme producing bacterium, Pseudomonas putida MTCC 7525, was isolated and subjected to optimization of laccase production. Maximum production (94.10. U/ml) was achieved at 30. °C and pH 8 (108. h incubation) with 10% skim milk and 1. mM sodium nitrate as additional nitrogen source. The laccase was purified by salt precipitation followed by ion exchange chromatography which showed 6.44 fold purification. The purified enzyme had optimal activity at pH 8.0 and 40. °C, and showed stability in DMSO retaining more than 85% of original activity. In presence of manganese and cadmium, enzyme retained >95% and 92% activity, respectively. The molecular weight of laccases was 39.5. kDa and activity was inhibited by pCMB (93%). The synthetic dyes (0.02%) and industrial effluents (10%) were decolorized to 74-93 and 58-68%, respectively when treated with culture of P. putida. However, the culture supernatant of P. putida showed about 36-94 and 16-86% decolorization of synthetic dyes and effluents, respectively within 24. h of incubation. © 2013 Elsevier Ltd. Source

Behari P.C.,Birla Institute of Technology | Bhardwaj A.K.,SHIATS
Research Journal of Applied Sciences, Engineering and Technology

The traditional water mills have low output with primarily use of grinding. The up gradation and improvement of these water mills is an effective and sustainable way of meeting the energy needs of a major section of the hilly people. This study discusses the case study of standard water mills developed in order to cover the entire range of water mills sites in Uttaranchal. Based on the site data available, 1 standard water mills have been designed, developed and tested for their performance. The developed water mills at Kherola village near Bhimtal, district Nainital have been found efficient. The status of water mills, energy situation in Uttarakhand, impact of water mill upgrade and the developed system have also been discussed. Source

Field trials were conducted to study the comparative bio-efficacy of some indigenous products (cow urine + cow dung, chilli-garlic extract and farmyard manure + neemcake with endosulfan as chemical check against the major insect pest diamondback moth (DBM), Plutella xylostella Linn., on cabbage c.v Golden Acre In all the three treatment applications, observation at 3, 6 and 9 days after spraying revealed that treatment with chilli-garlic extract was found most effective in reducing the insect population followed by treatment with cow urine + cow dung. Endosulfan as chemical check was superior to FYM + neemcake and FYM alone but these two treatments were found superior to control (untreated check). Consequently, chili-garlic extract proved to be most outstanding indigenous products, which gave best control against DBM while FYM was least effective. Source

Kuddus M.,Hail University | Singh P.,SHIATS | Thomas G.,SHIATS | Al-Hazimi A.,Hail University
BioMed Research International

An extensive range of pigments including phycobiliproteins are present in algae. C-phycocyanin (C-PC), a phycobiliprotein, is one of the key pigments of Spirulina, a microalgae used in many countries as a dietary supplement. Algal pigments have massive commercial value as natural colorants in nutraceutical, cosmetics, and pharmaceutical industries, besides their health benefits. At present, increasing awareness of harmful effects of synthetic compounds and inclination of community towards the usage of natural products have led to the exploitation of microalgae as a source of natural pigments/colors. This review describes recent findings about the sources and production of C-PC, with emphasis on specific techniques for extraction and purification, along with potential industrial applications in diagnostics, foods, cosmetics, and pharmaceutical industries. © 2013 M. Kuddus et al. Source

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