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Liang P.-Y.,Fertility Center | Liang P.-Y.,Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics | Diao L.-H.,Fertility Center | Diao L.-H.,Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics | And 14 more authors.
Reproductive BioMedicine Online | Year: 2015

Limited information is available on the balance state of pro- and anti-inflammatory cytokines in patients with recurrent implantation failure (RIF). This study assessed the pro- and anti-inflammatory cytokines in plasma of 34 patients with RIF, compared with those of 25 women with a successful pregnancy in the first IVF/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) cycle. The IFN-γ, IL-1β, IL-6 and IL-4 concentrations were higher, whereas the TGF-β1 concentration was lower in the RIF group compared with the control group. Furthermore, the ratios of pro-inflammatory and anti-inflammatory cytokines IFN-γ/IL-4, IFN-γ/IL-10, IFN-γ/TGF-β1, IL-6/IL-10, IL-6/TGF-β1, IL-1β/TGF-β1 and TNF-α/TGF-β1 were higher in the RIF group (all P < 0.01). The results suggested a shift toward a pro-inflammatory state in peripheral blood of the patients with RIF. © 2015. Source

Huang C.,Fertility Center | Huang C.,Shenzhen Key Laboratory of Reproductive Immunology for Peri implantation | Huang C.,Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics | Liang P.,Fertility Center | And 20 more authors.
International Journal of Environmental Research and Public Health | Year: 2015

Thyroid autoimmunity (TAI), which is defined as the presence of autoantibodies against thyroid peroxidase (TPO) and/or thyroglobulin (TG), is related to repeated implantation failure (RIF). It is reported that TAI was involved in reproductive failure not only through leading thyroid function abnormality, but it can also be accompanied with immune imbalance. Therefore, this study was designed to investigate the association of thyroid function, immune status and TAI in women with RIF. Blood samples were drawn from 72 women with RIF to evaluate the prevalence of TAI, the thyroid function, the absolute numbers and percentages of lymphocytes. The prevalence of thyroid function abnormality in RIF women with TAI was not significantly different from that in RIF women without TAI (χ2 = 0.484, p > 0.05). The absolute number and percentage of T cells, T helper (Th) cells, B cells and natural killer (NK) cells were not significantly different in RIF women with TAI compared to those without TAI (all p > 0.05). The percentage of T cytotoxicity (Tc) cells was significantly decreased in RIF women with TAI compared to those without TAI (p < 0.05). Meanwhile, Th/Tc ratio was significantly increased (p < 0.05). These results indicated that the decreased Tc percentage and increased Th/Tc ratio may be another influential factor of adverse pregnancy outcomes in RIF women with TAI. © 2015 by the authors; licensee MDPI, Basel, Switzerland. Source

Liang P.,Clinical Center for Recurrent Spontaneous Abortion | Liang P.,Fertility Center | Liang P.,Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics | Mo M.,Clinical Center for Recurrent Spontaneous Abortion | And 23 more authors.
American Journal of Reproductive Immunology | Year: 2012

Problem: The efficacy of lymphocyte immunotherapy (LIT) for unexplained recurrent miscarriage (uRM) remains indefinite. The objective of the present study is to comprehensively evaluate the alterations in the proportions and functions of peripheral blood lymphocytes in patients with uRM before and after LIT. Method of study: Seventy-six women with uRM were included in the present study. Immunophenotype of peripheral blood lymphocytes (76 patients), NK cytotoxicity (62 patients) and Th1 (IFN-γ and TNF-α)/Th2 (IL-10) ratios (73 patients) were assessed during the luteal phase before LIT and after the third LIT by the flow cytometer. Results: To date, 29 patients had already delivered or been pregnant more than 20 weeks (successful pregnancy group) and 5 patients experienced subsequent abortion (abortion group). The percentages of CD3 + T cell, CD3 + CD4 + T cell and IL-10-producing Th1 cell were significantly increased, whereas the percentages of CD3 + HLA-DR + T cell, CD56 + CD3 - NK cell and CD56 dimCD16 + NK cell, the NK cytotoxicity and the ratios of Th1/Th2 were significantly decreased after LIT in the total patients and in the successful pregnant group. The percentages of CD56 +CD3 - NK cell and IFN-γ-producing cell, and the ratio of IFN-γ/IL-10 were significantly lower after LIT in the successful pregnant group compared to those in the abortion group. Conclusions: LIT alters the proportions and functions of most peripheral blood lymphocyte subsets. Some of these alterations may be beneficial for pregnancy maintenance, whereas some may be potential markers for predicting subsequent abortion. © 2012 John Wiley & Sons A/S. Source

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