Chen B.,Shenzhen Traditional Chinese Medicine Hospital |
Xu L.-F.,Sun Yat Sen University |
Sun H.-L.,Sun Yat Sen University |
Chen Y.-T.,Sun Yat Sen University |
And 2 more authors.
Chinese Journal of Interventional Imaging and Therapy | Year: 2015
Objective: To evaluate the radiopacity, embolic effect and biocompatibility of lipiodol polylactic acid (LP-PLA) microspheres in the setting of rabbit renal artery embolism. Methods: Totally 36 healthy mature New Zealand white rabbits were randomly divided into two groups: LP-PLA group (left renal artery embolism by LP-PLA microspheres) and PVA group (left renal artery embolism by PVA particles). Six big white rabbits in each group were randomly selected to perform the radiography, and blood routine and biochemistry tests (including hepatic and renal function) were measured at 1, 4 and 12 weeks after embolism. Pathological features on the left and right kidney were compared. Results: LP-PLA microspheres mixing with normal saline could pass the pipe smoothly and its track under fluoroscopy monitor could be observed 1 week after embolism. Renal arteries were hot recanalized by angiography in each group at 1, 4 and 12 weeks after embolism. In both groups, the weight of the embolic kidney were significantly increased, compared with that of the healthy kidney at 1 week after embolism (P<0.05); the embolic kidneys obviously atrophied and the healthy kidneys showed compensatory hypertrophy at 4, 12 weeks after embolism (P<0.05). The pathology of embolic kidneys showed thrombogenesis and organization, and the foreign body giant cells and inflammation reactions around the embolic artery in LP-PLA group were more obvious than those in PVA group. Conclusion: LP-PLA microspheres demonstrate good radiopacity as well as histocompatibility in animal experiment, and it also shares similar long-time embolic effect with PVA. Copyright © 2015 by the Press of Chinese Journal of Medical Imaging and Technology.
Wang Y.-Y.,Shenzhen Traditional Chinese Medicine Hospital |
Liao S.-H.,Peking University |
Zhang S.-N.,Shenzhen Traditional Chinese Medicine Hospital
International Eye Science | Year: 2012
AIM: To observe the influence of free iron, synthetic or biological iron chelating agent on the formation of pseudomonas aeruginosa biofilm on the contact lenses. METHODS: Different concentration of free iron, different concentration and variety classes of synthesis iron and biological iron chelating agent were added into pseudomonas aeruginosa biofilm, and then the clump count on the biofilm was detected. RESULTS: Low concentration of free iron increased the adhesive capacity of the bacterial, with 10μmol/L of iron had the most obvious effect. 0.1μmol/L of EDTA and lactoferrin significantly inhibited the bacterial count of pseudomonas aeruginosa biofilm. CONCLUSION: Low concentration of free iron can promote the bacterial count of pseudomonas aeruginosa biofilm on the contact lens, the lack iron circumstance which formed by finite concentration lactoferrin and EDTA can prevent the formation of the biofilm.
Autophagy inhibitors promoted aristolochic acid I induced renal tubular epithelial cell apoptosis via mitochondrial pathway but alleviated nonapoptotic cell death in mouse acute aritolochic acid nephropathy model
Zeng Y.,Sun Yat Sen University |
Zeng Y.,Shenzhen Traditional Chinese Medicine Hospital |
Li S.,Shenzhen Traditional Chinese Medicine Hospital |
Wu J.,Sun Yat Sen University |
And 5 more authors.
Apoptosis | Year: 2014
Aristolochic acid I (AAI) can induce renal tubular epithelial cells (RTECs) autophagy, which thereby extenuates apoptosis in vitro. In this study, we aimed to determine whether the in vitro data also apply to the AAI-induced pathologic condition in vivo. BALB/c mice were treated with AAI, autophagy inhibitors [3-methyladenine (3MA) or chloroquine diphosphate salt (CQ)], and AAI plus the inhibitors for consecutive 5 days, respectively. Mice were euthanized on day 3 and 5. AAI induced RTECs autophagy was confirmed by electron microscopy and western blot. The results showed induction of apoptotic RTECs and up-regulation of mitochondrial and endoplasmic reticulum stress-related proteins in AAI-treated mice at both of the two time points. There were more apoptotic RTECs in AAI + inhibitor groups, which might be due to increased mitochondrial stress-related proteins (cytochrome C and apoptotic protease activating factor 1, APAF-1). On day 5, severe tubulointerstitial injuries induced by AAI led to a significant decline in kidney function. There were numerous autolysosomes in dying RTECs of the AAI group. Autophagy inhibitors increased AAI-induced RTECs mitochondrial apoptosis by increasing mitochondrial stress-related proteins, but they partially mitigated the AAI-induced severe renal tubulointerstitial injury. These results confirmed that AAI could induce autophagy in RTECs, which prevented apoptosis via mitochondrial pathway in vivo. However, continuous stimulation with AAI induced excess autophagy, which ultimately resulted in AAI-induced cell death. It suggested that apoptosis wasn't the main culprit in acute aristolochic acid nephropathy mice model. © 2014 Springer Science+Business Media.
Weng G.,Southern Medical University |
Zeng Y.,Jiangmen Wuyi Traditional Chinese Medicine Hospital |
Huang J.,Shenzhen Traditional Chinese Medicine Hospital |
Fan J.,Jiangmen Wuyi Traditional Chinese Medicine Hospital |
Guo K.,Southern Medical University
BioMed Research International | Year: 2015
Leukemia relapse and nonrecurrence mortality (NRM) due to leukemia stem cells (LSCs) represent major problems following hematopoietic stem cell transplantation (HSCT). To eliminate LSCs, the sensitivity of LSCs to chemotherapeutic agents used in conditioning regimens should be enhanced. Curcumin (CUR) has received considerable attention as a result of its anticancer activity in leukemia and solid tumors. In this study, we investigated the cytotoxic effects and underlying mechanisms in leukemia stem-like KG1a cells exposed to busulfan (BUS) and CUR, either alone or in combination. KG1a cells exhibiting BUS-resistance demonstrated by MTT and annexin V/propidium iodide (PI) assays, compared with HL-60 cells. CUR induced cell growth inhibition and apoptosis in KG1a cells. Apoptosis of KG1a cells was significantly enhanced by treatment with CUR+BUS, compared with either agent alone. CUR synergistically enhanced the cytotoxic effect of BUS. Seven apoptosis-related proteins were modulated in CUR-And CUR+BUS-Treated cells analyzed by proteins array analysis. Importantly, the antiapoptosis protein survivin was significantly downregulated, especially in combination group. Suppression of survivin with specific inhibitor YM155 significantly increased the susceptibility of KG1a cells to BUS. These results demonstrated that CUR could increase the sensitivity of leukemia stem-like KG1a cells to BUS by downregulating the expression of survivin. © 2015 Guangyang Weng et al.
PubMed | Jiangmen Wuyi Traditional Chinese Medicine Hospital, Southern Medical University and Shenzhen Traditional Chinese Medicine Hospital
Type: | Journal: BioMed research international | Year: 2015
Leukemia relapse and nonrecurrence mortality (NRM) due to leukemia stem cells (LSCs) represent major problems following hematopoietic stem cell transplantation (HSCT). To eliminate LSCs, the sensitivity of LSCs to chemotherapeutic agents used in conditioning regimens should be enhanced. Curcumin (CUR) has received considerable attention as a result of its anticancer activity in leukemia and solid tumors. In this study, we investigated the cytotoxic effects and underlying mechanisms in leukemia stem-like KG1a cells exposed to busulfan (BUS) and CUR, either alone or in combination. KG1a cells exhibiting BUS-resistance demonstrated by MTT and annexin V/propidium iodide (PI) assays, compared with HL-60 cells. CUR induced cell growth inhibition and apoptosis in KG1a cells. Apoptosis of KG1a cells was significantly enhanced by treatment with CUR+BUS, compared with either agent alone. CUR synergistically enhanced the cytotoxic effect of BUS. Seven apoptosis-related proteins were modulated in CUR- and CUR+BUS-treated cells analyzed by proteins array analysis. Importantly, the antiapoptosis protein survivin was significantly downregulated, especially in combination group. Suppression of survivin with specific inhibitor YM155 significantly increased the susceptibility of KG1a cells to BUS. These results demonstrated that CUR could increase the sensitivity of leukemia stem-like KG1a cells to BUS by downregulating the expression of survivin.
PubMed | Chinese University of Hong Kong, Shenzhen Traditional Chinese Medicine Hospital and Hong Kong Polytechnic University
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2016
This study aimed to investigate the effects of Shugan Xiaozhi decoction (SX) on nonalcoholic steatohepatitis (NASH) induced by high-fat diet in rats. The rats were randomly divided into 6 groups, namely, control, model, fenofibrate, and three different dosage of SX (10, 20, and 40g/kg/day, p.o.). After establishing the NASH model, at 8 weeks of the experiment, treatments were administrated intragastrically to the fenofibrate and SX groups. All rats were killed after 4 weeks of treatment. Compared with the model group, alanine aminotransferase (ALT), aspartate aminotransferase (AST), free fatty acid (FFA), total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein cholesterol (LDL) serum in the serum were significantly reduced in all SX treatment groups in a dose-dependent manner. Evidence showed that SX could protect the liver by upregulating the gene and protein expressions of peroxisome proliferator-activated receptor alpha (PPAR
Ruolan H.,Shenzhen Traditional Chinese Medicine Hospital |
Zhong Z.,Shenzhen Traditional Chinese Medicine Hospital |
Mujuan X.,Shenzhen Traditional Chinese Medicine Hospital |
Xiao C.,Shenzhen Traditional Chinese Medicine Hospital |
And 3 more authors.
Chinese Critical Care Medicine | Year: 2014
Objective: To investigate the effects of Sini decoction on function of hypothalamic-pituitary-adrenal axis in patients with sepsis. Methods: A prospective single-blind randomized controlled trial was conducted. 60 septic patients were divided into three groups with the method of random number table, 20 cases in the control group, 20 in the Chinese herb group, and 20 in corticoid group. All of them received routine treatment. Patients in Chinese herb group were given Sini decoction in addition (decoction of monkshood 15 g, dried ginger 15 g, honey-fried licorice 10 g) 100 mL/d orally or by nasal feeding, while patients in corticoid group were given hydrocortisone 200 mg/d intravenously instead, both for 7 days. Before the treatment, 3 days and 14 days after treatment, blood was collected to determine the levels of adrenocorticotropic hormone (ACTH) and cortisol, and the result of ACTH stimulating test was observed. At the same time, acute physiology and chronic health evaluation II (APACHE II) score was recorded, and 3-day shock recovery rate and 28-day death rate were also compared among these groups. Results: None of the three groups showed different result in ACTH stimulating test (χ2=1.101, P=0.605). ACTH in three groups was gradually decreased. Compared with that before treatment, ACTH in Chinese herb group and corticoid groups began to decrease obviously on day 3(ng/L: 29.90 ± 3.31 vs. 33.10 ± 3.31, 28.20 ± 2.45 vs. 33.30 ± 3.84, both P<0.01), while in control group declined ACTH appeared later (on day 14) compared with before treatment (ng/L: 29.40 ± 5.63 vs. 33.50 ± 4.89, P<0.05). No obvious difference in ACTH level was showed between the Chinese herb group and the cortical group (both P>0.05). Cortisol level in both Chinese herb and cortical groups showed a raise-fall biphase trend while there was no change in the control. The cortical levels on day 3 in Chinese herb and cortical groups were much higher than that before treatment (μg/L: 343.04 ± 31.20 vs. 294.70 ± 42.10, 331.25 ± 42.80 vs. 280.36 ± 38.10, both P<0.01) and that of control group (μg/L: 291.61 ± 41.50, both P<0.01), though no significant statistical difference was observed between two groups (both P>0.05). APACHE II score on day 14 in control, Chinese herb and cortical groups was significantly lower than that before treatment (16.8 ± 5.1 vs. 20.1 ± 4.3, 13.4 ± 3.2 vs. 18.3 ± 3.8, 15.1 ± 2.5 vs. 19.5 ± 4.0, all P<0.01), and the score was much lower in Chinese herb group comparing with that of control group (P<0.05). No statistical difference was observed among control, Chinese herb and cortical groups in lowering 28-day death rate [35.0% (7/20), 25.0% (5/20), 20.0% (4/20)] and improving 3-day shock recovery rate [40.0% (8/20), 70.0% (14/20), 60.0% (12/20), all P>0.05). Conclusions: Sini decoction could elevate cortisol while lower ACTH at the early stage of sepsis. Sini decoction could also effectively improve symptoms and hypothalamic-pituitary-adrenal axis function in septic patients without affecting death rate.
PubMed | Shenzhen Traditional Chinese Medicine hospital and CAS Shenzhen Institutes of Advanced Technology
Type: Journal Article | Journal: Clinical hemorheology and microcirculation | Year: 2016
Bells palsy is a kind of peripheral neural disease that cause abrupt onset of unilateral facial weakness. In the pathologic study, it was evidenced that ischemia of facial nerve at the affected side of face existed in Bells palsy patients. Since the direction of facial nerve blood flow is primarily proximal to distal, facial skin microcirculation would also be affected after the onset of Bells palsy. Therefore, monitoring the full area of facial skin microcirculation would help to identify the condition of Bells palsy patients. In this study, a non-invasive, real time and full field imaging technology - laser speckle imaging (LSI) technology was applied for measuring facial skin blood perfusion distribution of Bells palsy patients. 85 participants with different stage of Bells palsy were included. Results showed that Bells palsy patients facial skin perfusion of affected side was lower than that of the normal side at the region of eyelid, and that the asymmetric distribution of the facial skin perfusion between two sides of eyelid is positively related to the stage of the disease (P< 0.001). During the recovery, the perfusion of affected side of eyelid was increasing to nearly the same with the normal side. This study was a novel application of LSI in evaluating the facial skin perfusion of Bells palsy patients, and we discovered that the facial skin blood perfusion could reflect the stage of Bells palsy, which suggested that microcirculation should be investigated in patients with this neurological deficit. It was also suggested LSI as potential diagnostic tool for Bells palsy.
PubMed | Shenzhen Traditional Chinese Medicine Hospital
Type: Journal Article | Journal: Nutrition research and practice | Year: 2014
The objectives of this study were to investigate the effects of lycopene on the migration, adhesion, tube formation capacity, and p38 mitogen-activated protein kinase (p38 MAPK) activity of endothelial progenitor cells (EPCs) cultivated with high glucose (HG) and as well as explore the mechanism behind the protective effects of lycopene on peripheral blood EPCs.Mononuclear cells were isolated from human peripheral blood by Ficoll density gradient centrifugation. EPCs were identified after induction of cellular differentiation. Third generation EPCs were incubated with HG (33 mmol/L) or 10, 30, and 50 g/mL of lycopene plus HG. MTT assay and flow cytometry were performed to assess proliferation and apoptosis of EPCs. EPC migration was assessed by MTT assay with a modified boyden chamber. Adhesion assay was performed by replating EPCs on fibronectin-coated dishes, after which adherent cells were counted. In vitro vasculogenesis activity was assayed by Madrigal network formation assay. Western blotting was performed to analyze protein expression of both phosphorylated and non-phosphorylated p38 MAPK.The proliferation, migration, adhesion, and in vitro vasculogenesis capacity of EPCs treated with 10, 30, and 50 g/mL of lycopene plus HG were all significantly higher comapred to the HG group (P < 0.05). Rates of apoptosis were also significantly lower than that of the HG group. Moreover, lycopene blocked phosphorylation of p38 MAPK in EPCs (P < 0.05). To confirm the causal relationship between MAPK inhibition and the protective effects of lycopene against HG-induced cellular injury, we treated cells with SB203580, a phosphorylation inhibitor. The inhibitor significantly inhibited HG-induced EPC injury.Lycopene promotes proliferation, migration, adhesion, and in vitro vasculogenesis capacity as well as reduces apoptosis of EPCs. Further, the underlying molecular mechanism of the protective effects of lycopene against HG-induced EPC injury may involve the p38 MAPK signal transduction pathway. Specifically, lycopene was shown to inhibit HG-induced EPC injury by inhibiting p38 MAPKs.
PubMed | Shenzhen Traditional Chinese Medicine Hospital
Type: Journal Article | Journal: Wei sheng yan jiu = Journal of hygiene research | Year: 2012
To investigate the effect of lycopene treatment on the proliferation and apoptosis of endothelial progenitor cells (EPCs) incubated in a culture medium with high concentration of glucose.Mononuclear cells (MNCs) were isolated from human peripheral blood by Ficoll density gradient centrifugation. After being induced to differentiation, the endothelial progenitor cells (EPC) were identified by FITC-labeled Ulex europaeus agglutinin I and Dil labeled acetylated low density lipoprotein dual stain method. Then MTT assay and flow cytometry were used to assess the proliferation and apoptosis of EPCs.The glucose in a concentration of 33 mmol/L significantly inhibited the proliferation and promoted the apoptosis of EPCs (P < 0.05). The proliferation of EPCs in 10, 30 and 50 microg/ml lycopene groups were significantly higher than the 0 microg/ml group. The rate of apoptosis were significantly lower than the lycopene 0 microg/ml group (P < 0.05).High concentration of glucose attenuates the proliferative activity and increases the apoptotic rate of EPCs. Lycopene promotes the proliferation and reduces the apoptosis of EPCs cultivated in high glucose medium.