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Li W.,Anhui Medical College | Li W.,Shenzhen Tissue Engineering Laboratory | Zhao Z.,Anhui Medical College | Xiong J.,Anhui Medical College | Zeng Y.,Beijing University of Technology
Artificial Cells, Nanomedicine and Biotechnology

Objective: To study the feasibility of therapy using nano-bone gelatin to comminuted fracture by animal experiment. Methods: The animal models of transverse fracture were made on bilateral ulnas of 45 New Zealand white rabbits, which were divided into experimental group (repair with nano-bone gelatin), control group (repair with traditional medical glue), and blank group (unrepaired) randomly. The reconstruction effect in each group was evaluated using X-ray examination, MicroCT scanning, histopathology observation, and biomechanical test postoperation regularly. Result: On 12th week, the fractures in experimental and blank group get the marrow cavity completely unobstructed and good bone union; however, in control group, the fracture line slightly blurred with the marrow cavity not fully unobstructed; 6 weeks later, observation of bony calluses through MircoCT: experimental group, 68.5 ± 2.71%; blank group, 69.19 ± 2.3%; and control group, 49.35 ± 3.56%, there were no significant difference between the two groups (P > 0.05). The control group obviously showed worse bone union than the former two (P < 0.05). The histopathological examination shows that the bony calluses of experimental group are similar to those of the blank group; however, gelatin degraded slowly in control group with delayed union; on the 12th week, biomechanical test shows that the blank and experiment groups had basically same average bending strength values which had no significant difference (P > 0.05) and obviously were higher than those of the control group (P < 0.01). Conclusion: The nano-bone gelatin won't lead to delayed union of fractures and may be beneficial to it, and so may be an ideal gelatin for fixing small fractures. © 2014 Informa Healthcare USA, Inc. Source

Chen J.-L.,Sun Yat Sen University | Chen J.-L.,Shenzhen Tissue Engineering Laboratory | Zhu W.,Anhui Medical University | Zhu W.,Shenzhen University | And 16 more authors.
Artificial Cells, Nanomedicine and Biotechnology

Objective: To study the cellular compatibility of the magnetic nanocomposite (n-HA/PLLA/Fe2O3) and to discuss the biological behaviors of cells including surface adhesion, proliferation, and expression. The present work provides an experimental basis for medical application. Method: Rat osteoblasts (OB) were co-cultured with the magnetic nanocomposite. Cell proliferation, cell adhesion, and the expression of type I collagen (Col-I) and osteocalcin (OCN) gene were characterized by the cell counting kit-8 (CCK-8) method, scanning electronic microscopy (SEM), and reverse transcription polymerase chain reaction (RT-PCR), respectively. Results: CCK-8 detection showed that there was no difference in cell proliferation on the magnetic nanocomposite between the experimental group and control group (P > 0.05). SEM indicated that a large amount of cells adhered to the surface and in the pores of the magnetic nanocomposite. As the co-culture time increased, the cells adhering to the magnetic nanocomposite showed an obvious increase. RT-PCR detection showed that as the co-culture time increased, the expression of the Col-I gene was enhanced (P < 0.05). There was no obvious difference in the expression of the OCN gene (P > 0.05). Conclusion: The magnetic nanocomposite is suitable for cell adhesion, growth, and differentiation with a high cellular compatibility. © 2015 Informa Healthcare USA, Inc. Source

Zhu W.,Anhui Medical University | Zhu W.,Guangzhou University | Guo D.,Anhui Medical University | Guo D.,Guangzhou University | And 11 more authors.
Artificial Cells, Nanomedicine and Biotechnology

Objective: When preliminary tests have confirmed a nano-hydroxyapatite (Nano-HA) content of 20% of the polylactic acid (PLA) composite material of Nano-HA interface fixation material for biomechanical requirements, there is a need for further observation of its biocompatibility and clinical applications, to provide reference data. Methods: Preparation of Nano-HA content of 20% PLA composite Nano-HA bone substitute material and extract. The establishment of the negative control group (containing 10% fetal bovine serum in DMEM complete medium), experimental group (extract), the positive control group (mass concentration of 0.64% phenol), and a co-culture of rabbit bone marrow mesenchymal stem cells (rBMSC) and materials extraction liquid. Observation of the morphological changes in rBMSC in culture at time points of 3, 5, and 7 days, the use of the MTT assay, and determination of the relative growth in the above set of rBMSC in cell culture at 3, 5, and 7 days, to judge the material's cytotoxicity. Results: With time, the absorbance value of the three groups of cells were significantly increased (P < 0.01). The relative growth of the rBMSCs in experimental group in the first 3, 5, and 7 days was 95.3%, 96.8% and 97.6%; the cytotoxicity was according to the national standards I; the difference was not significant (P > 0.05) between the the experimental group and the negative control group; there was a significant difference between the positive control group and the other 2 groups (P < 0.05). Cells in the experimental group were seen having normal morphology, and spindle-shaped adherent growth. Conclusion: PLA composite artificial bone materials and Nano-HA show good cell compatibility, and the values for cytotoxicity, with reference to GB/T16886.5.2003 (China) standards, are in the safe range. © 2015 Informa Healthcare USA, Inc. Source

Zhu W.,Shenzhen University | Zhu W.,Shenzhen Tissue Engineering Laboratory | Zhu W.,Shenzhen Sports Medicine Engineering Laboratory | Zhu W.,Guangzhou University | And 17 more authors.
European Journal of Trauma and Emergency Surgery

Aim: To evaluate the outcome of arthroscopy treatment using high-strength line in the treatment of tibial avulsion fracture of posterior cruciate ligament. Methods: Both the avulsed bone block and the tibia bone bed were refreshed. The procedure was completed with the assistance of PCL director drill guide. The reduction and fixation using high-strength line were used to fix the avulsed bone by from posterior middle portal. Rehabilitation began early postoperatively. Results: From January 2010 to June 2012, a total of 18 arthroscopically treated cases of PCL tibial avulsion fracture were retrospectively evaluated. Reduction of the avulsion fragment was obtained in all cases. 16 cases were followed up for 7–30 months (average 13.6), and 2 cases were out of follow-up. In the 16 followed patients, flexion and extension were back to normal within 6 weeks, and return to normal walk in 12 weeks. The bone healing was good without any vascular or nerve complications. All the patients regained the preinjury activity level. The mean score (and standard deviation) increased from 38.9 ± 4.9 points to 95.2 ± 3.8 points with the system of Lysholm, from 57.1 ± 10.3 points to 94.3 ± 4.4 points with the system of IKDC. Post-test displacement of KT3000 declined from 3.6 ± 0.39 to 1.1 ± 0.27 mm. Conclusion: Arthroscopic vertical fixation by high-strength line is a simple, safe, reliable, and micro-invasive treatment to PCL tibial avulsion fracture. It is a kind of real all arthroscopic technique, and good for early postoperative rehabilitation. The total stability of the knee could be gained, and the second operation to remove the internal fixation is avoided. © 2015 The Author(s) Source

Zhu W.,Guangzhou University | Zhu W.,Shenzhen University | Zhu W.,Shenzhen Tissue Engineering Laboratory | Zhu W.,Shenzhen Digital Orthopaedic Engineering Laboratory | And 15 more authors.
Artificial Cells, Nanomedicine and Biotechnology

Objective : To study the clinical effect of bone defect treated with nano-hydroxyapatite(Nano-HA) artificial bone. Methods : From September 2009 to June 2012, 27 cases of bone defect were analyzed retrospectively. The position of bone defect included humerus, radius, ulna, femur, tibia and calcaneus. The range of bone defect was from 0.3 ± 1.0 cm to 3 ± 6.5 cm. Among them, there were 22 cases with fractures and 5 cases with tumors. All patients were treated with Nano-HA artifi cial bone. The ability of bone defect repair was evaluated by X-ray exams performed preoperatively and postoperatively. HSS scores were adopted for fi nal evaluation at the latest follow-up. Results : The patients were followed up from 11 to 26 months (average of 18.5 months). No general side eff ects occurred. X-ray photo showed an integrity interface between Nano-HA and bone. Primary healing was obtained in all cases without any complication. Conclusion : The Nano-HA artifi cial bone had a good biocompatibility and could be an ideal artifi cial bone in the reconstruction of bone defect. Copyright © 2014 Informa Healthcare USA, Inc. Source

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