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Zhu W.,Shenzhen University | Zhu W.,Shenzhen Tissue Engineering Laboratory | Zhu W.,Shenzhen Sports Medicine Engineering Laboratory | Zhu W.,Guangzhou University | And 17 more authors.
European Journal of Trauma and Emergency Surgery | Year: 2015

Aim: To evaluate the outcome of arthroscopy treatment using high-strength line in the treatment of tibial avulsion fracture of posterior cruciate ligament. Methods: Both the avulsed bone block and the tibia bone bed were refreshed. The procedure was completed with the assistance of PCL director drill guide. The reduction and fixation using high-strength line were used to fix the avulsed bone by from posterior middle portal. Rehabilitation began early postoperatively. Results: From January 2010 to June 2012, a total of 18 arthroscopically treated cases of PCL tibial avulsion fracture were retrospectively evaluated. Reduction of the avulsion fragment was obtained in all cases. 16 cases were followed up for 7–30 months (average 13.6), and 2 cases were out of follow-up. In the 16 followed patients, flexion and extension were back to normal within 6 weeks, and return to normal walk in 12 weeks. The bone healing was good without any vascular or nerve complications. All the patients regained the preinjury activity level. The mean score (and standard deviation) increased from 38.9 ± 4.9 points to 95.2 ± 3.8 points with the system of Lysholm, from 57.1 ± 10.3 points to 94.3 ± 4.4 points with the system of IKDC. Post-test displacement of KT3000 declined from 3.6 ± 0.39 to 1.1 ± 0.27 mm. Conclusion: Arthroscopic vertical fixation by high-strength line is a simple, safe, reliable, and micro-invasive treatment to PCL tibial avulsion fracture. It is a kind of real all arthroscopic technique, and good for early postoperative rehabilitation. The total stability of the knee could be gained, and the second operation to remove the internal fixation is avoided. © 2015 The Author(s)


Wang D.-P.,Shenzhen Second Peoples Hospital | Wang D.-P.,Shenzhen Tissue Engineering Laboratory | Xiong J.-Y.,Shenzhen Second Peoples Hospital | Xiong J.-Y.,Shenzhen Tissue Engineering Laboratory | And 6 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Nano-hydroxyapatite helps to improve the mechanical properties of bone implants. Objective: To study the clinical effect of nano-hydroxyapatite artificial bone on collapsed fracture of the tibial plateau. Methods: Fourteen cases of collapsed fracture of the tibial plateau combined with bone defects from March 2010 to September 2012 were analyzed retrospectively. The bone defect range was from 1.5 cm×1.0 cm to 3.1 cm×4.5 cm. All patients were treated with nano-hydroxyapatite artificial bone at an implant amount of 5-14 g. Clinical and X-ray observations were applied at 1 week, 1 month and 3 months postoperatively. Hospital for Special Surgery scores were employed for recovery of knee function. Results and Conclusion: The patients were followed up for 12-27 months. Except for one case of a small amount of wound exudates, no general side effects occurred in 13 cases. X-ray photo showed an integrity interface between nano-hydroxyapatite artificial bone and host bone at 3 months after treatment. Primary healing was obtained in all cases without any complications. Hospital for Special Surgery score was increased to (88.7±4.3) points at 1 year later. These findings indicate that the nano-hydroxyapatite artificial bone has a good biocompatibility and biomechanics, and it may be an ideal artificial bone for repairing collapsed fractures of the tibial plateau.


Zhu W.,Anhui Medical University | Zhu W.,Guangzhou University | Guo D.,Anhui Medical University | Guo D.,Guangzhou University | And 11 more authors.
Artificial Cells, Nanomedicine and Biotechnology | Year: 2016

Objective: When preliminary tests have confirmed a nano-hydroxyapatite (Nano-HA) content of 20% of the polylactic acid (PLA) composite material of Nano-HA interface fixation material for biomechanical requirements, there is a need for further observation of its biocompatibility and clinical applications, to provide reference data. Methods: Preparation of Nano-HA content of 20% PLA composite Nano-HA bone substitute material and extract. The establishment of the negative control group (containing 10% fetal bovine serum in DMEM complete medium), experimental group (extract), the positive control group (mass concentration of 0.64% phenol), and a co-culture of rabbit bone marrow mesenchymal stem cells (rBMSC) and materials extraction liquid. Observation of the morphological changes in rBMSC in culture at time points of 3, 5, and 7 days, the use of the MTT assay, and determination of the relative growth in the above set of rBMSC in cell culture at 3, 5, and 7 days, to judge the material's cytotoxicity. Results: With time, the absorbance value of the three groups of cells were significantly increased (P < 0.01). The relative growth of the rBMSCs in experimental group in the first 3, 5, and 7 days was 95.3%, 96.8% and 97.6%; the cytotoxicity was according to the national standards I; the difference was not significant (P > 0.05) between the the experimental group and the negative control group; there was a significant difference between the positive control group and the other 2 groups (P < 0.05). Cells in the experimental group were seen having normal morphology, and spindle-shaped adherent growth. Conclusion: PLA composite artificial bone materials and Nano-HA show good cell compatibility, and the values for cytotoxicity, with reference to GB/T16886.5.2003 (China) standards, are in the safe range. © 2015 Informa Healthcare USA, Inc.


Zhu W.,Guangzhou University | Zhu W.,Shenzhen Second People Hospital | Zhu W.,Shenzhen Tissue Engineering Laboratory | Huang J.,Shenzhen Tissue Engineering Laboratory | And 11 more authors.
Artificial Cells, Nanomedicine and Biotechnology | Year: 2014

Objective: By in situ polymerization of poly-L-lactic acid (PLLA) and nano-hydroxyapatite (Nano-HA), and finding the best proportion of composite, so as to get ideal interface fixation material. Methods: According to a certain ratio (the mass fraction of Nano-HA, respectively, is 0%, 10%, 20%, 30% and 40%), composite PLLA and Nano-HA by in situ polymerization, and test the performance of this kind of new type of interface fixation such as, bending strength,compressive strength, elastic modulus, scanning electron microscopy (SEM) and degradation experiments in vitro. Then observe its mechanical properties, microstructure, the dispersion of Nano-HA in the PLLA and degradation rate of composite materials. Results: 1. Mechanical tests show that with the increase of Nano-HA content, the tensile strength decreases and the elastic modulus increases; with Nano-HA content of 20%, the bending strength of composite materials presents the peak value (156.8 MPa). 2. SEM scan shows the fracture surface of pure PLLA is relatively smooth; with Nano-HA content of 10%, the fracture surface shows a large number of dimples, and is obvious rough; with Nano-HA content of 20%, the fracture surface is uneven, forming a large number of dimples; with Nano-HA content of 30% or more, the fracture surface becomes more flat, and there are some small dimples. 3. Degradation experiments in vitro show the following: as the degradation time goes on, the pH values of degradation liquid is gradually reduced and the mechanical properties of composite materials also gradually have some decay. Conclusion: With Nano-HA content of 20%, the interface fixation material has a better mechanical properties and degradation properties. According to the best ratio, prepare Nano-HA/PLLA composite artificial materials with good performance. © 2014 Informa Healthcare USA, Inc.


Zhu W.,Guangzhou University | Zhu W.,Shenzhen Second People Hospital | Zhu W.,Shenzhen Tissue Engineering Laboratory | Chen K.,Guangzhou University | And 12 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2014

The aim of this study is to investigate the effects of differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) into chondrocytes induced by transforming growth factor-beta1 (TCP-β1) composite poly-1actide-co- glycolic acid/nano-hydroxyapatite (PLLA/nano-HA) to the construction of biomimetic artificial cartilage in vitro. In the low-temperature extrusion preparation of PLLA/nano-HA composite porous scaffolds, rBMSCs were isolated and cultured to third generation in vitro, induced by TGF-β1-contained special inducing system into chondrocytes, 14 d later, identified by toluidine blue and type II collagen immunohistochemistry staining, and then the differential chondrocytes composite into the PLLA/nano-HA composite porous scaffolds, using scanning electron microscopy (SEM) to observe the growth conditions and cell attachment on the composite in the 7th,14th, and 21st day and to gather cells on composite in the 7th, 14th, and 21st day of cell. RT-PCR is used to detect the expression of aggrecan (Col2A1 in mRNA) and Western blot for detection of the expression of type II collagen of the attached cells. rBMSCs can differentiate into chondrocytes when induced, and the differentiation of chondrocytes secreting GAG by toluidine blue staining and type II collagen immunohistochemistry staining was positive; SEM confirm the cells distribution evenly, stretching well in composite. RT-PCR of aggrecan, Col2A1 in mRNA, and Western-blot of type II collagen expression in the differentiation of chondrocytes have different levels. Using TGF-β1 containing special inducing system induced rBMSCs into chondrocytes, then into compounds of PLLA/nano-HA composite porous scaffolds, and cell carrier complex proliferated well and secreted the chondrocyte-specific extracellular matrix stably, successfully constructing artificial bionic in vitro. © 2013 The Society for In Vitro Biology.


Zhu W.,Guangzhou University | Zhu W.,Shenzhen University | Zhu W.,Shenzhen Tissue Engineering Laboratory | Zhu W.,Shenzhen Digital Orthopaedic Engineering Laboratory | And 15 more authors.
Artificial Cells, Nanomedicine and Biotechnology | Year: 2015

Objective : To study the clinical effect of bone defect treated with nano-hydroxyapatite(Nano-HA) artificial bone. Methods : From September 2009 to June 2012, 27 cases of bone defect were analyzed retrospectively. The position of bone defect included humerus, radius, ulna, femur, tibia and calcaneus. The range of bone defect was from 0.3 ± 1.0 cm to 3 ± 6.5 cm. Among them, there were 22 cases with fractures and 5 cases with tumors. All patients were treated with Nano-HA artifi cial bone. The ability of bone defect repair was evaluated by X-ray exams performed preoperatively and postoperatively. HSS scores were adopted for fi nal evaluation at the latest follow-up. Results : The patients were followed up from 11 to 26 months (average of 18.5 months). No general side eff ects occurred. X-ray photo showed an integrity interface between Nano-HA and bone. Primary healing was obtained in all cases without any complication. Conclusion : The Nano-HA artifi cial bone had a good biocompatibility and could be an ideal artifi cial bone in the reconstruction of bone defect. Copyright © 2014 Informa Healthcare USA, Inc.

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