Shenzhen Sixth Peoples Hospital
Shenzhen Sixth Peoples Hospital
Song Y.,Shenzhen University |
He L.,Shenzhen Sixth Peoples Hospital |
Zhou F.,University of Michigan |
Chen S.,Shenzhen University |
And 3 more authors.
IEEE Journal of Biomedical and Health Informatics | Year: 2017
Quantitative analysis of bacterial morphotypes in the microscope images plays a vital role in diagnosis of bacterial vaginosis (BV) based on the Nugent score criterion. However, there are two main challenges for this task: 1) It is quite difficult to identify the bacterial regions due to various appearance, faint boundaries, heterogeneous shapes, low contrast with the background, and small bacteria sizes with regards to the image. 2) There are numerous bacteria overlapping each other, which hinder us to conduct accurate analysis on individual bacterium. To overcome these challenges, we propose an automatic method in this paper to diagnose BV by quantitative analysis of bacterial morphotypes, which consists of a three-step approach, i.e., bacteria regions segmentation, overlapping bacteria splitting, and bacterial morphotypes classification. Specifically, we first segment the bacteria regions via saliency cut, which simultaneously evaluates the global contrast and spatial weighted coherence. And then Markov random field model is applied for high-quality unsupervised segmentation of small object. We then decompose overlapping bacteria clumps into markers, and associate a pixel with markers to identify evidence for eventual individual bacterium splitting. Next, we extract morphotype features from each bacterium to learn the descriptors and to characterize the types of bacteria using an Adaptive Boosting machine learning framework. Finally, BV diagnosis is implemented based on the Nugent score criterion. Experiments demonstrate that our proposed method achieves high accuracy and efficiency in computation for BV diagnosis. © 2013 IEEE.
Zhang X.,Nanjing University |
Huang Z.,Shenzhen Sixth Peoples Hospital |
Xie Y.,Nanjing University |
Chen X.,Nanjing University |
And 7 more authors.
International Journal of Neuroscience | Year: 2016
Background: Both adiponectin and endothelial progenitor cells (EPCs) have been proposed recently with anti-atherosclerosis effects. However, their impacts on vascular outcomes in patients with large artery atherosclerosis (LAA) are unclear. This study aimed to investigate the relationship between adiponectin, EPCs and stroke with a case-control design. Methods: The study cohort included 127 patients (61.3 ± 11.0 years; 73.2% men) with LAA stroke and 58 control subjects (60.9 ± 9.2 years; 70.7% men) referred for adiponectin and EPCs levels testing. We collected demographic, clinical, angiographical features, and laboratory data. Influence of adiponectin and EPCs levels on cerebral atherosclerosis and LAA stroke was analyzed with regression models. Results: The levels of adiponectin and EPCs in atherosclerotic stroke patients were significantly lower compared with matched controls (p < 0.05). Logistic regression analysis identified that reduced levels of adiponectin and EPCs were closely correlated with cerebral atherosclerosis and LAA stroke. The associations remained significant after adjustment for age, sex and other confounders. Additionally, partial correlation analysis revealed a significant positive association between adiponectin and three subpopulations of EPCs levels (CD34+CD133+CD309+cells: r = 0.510, p = 0.001; CD34+ CD133-CD309+cells: r = 0.262, p = 0.004; CD34-CD133+CD309+cells: r = 0.348, p < 0.001). Conclusions: Adiponectin is positively correlated with EPCs levels, and both of them are independently associated with LAA stroke. Copyright © 2015 Taylor and Francis.
Xiang Y.,Sun Yat Sen University |
Xiang Y.,Shenzhen Sixth Peoples Hospital |
Liu H.H.,Sun Yat Sen University |
Yan T.B.,Sun Yat Sen University |
And 3 more authors.
Neural Regeneration Research | Year: 2014
Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plasticity, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin (a neural precursor cell marker)-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic fibroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was significantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.
PubMed | Shenzhen Sixth Peoples Hospital, University of South Australia, Sun Yat Sen University and Guangdong University of Technology
Type: | Journal: Scientific reports | Year: 2015
Peripheral nerves are important pathways for receiving afferent sensory impulses and sending out efferent motor instructions, as carried out by sensory nerve fibers and motor nerve fibers. It has remained a great challenge to functionally reconnect nerve internal fiber bundles (or fascicles) in nerve repair. One possible solution may be to establish a 3D nerve fascicle visualization system. This study described the key technology of 3D peripheral nerve fascicle reconstruction. Firstly, fixed nerve segments were embedded with position lines, cryostat-sectioned continuously, stained and imaged histologically. Position line cross-sections were identified using a trained support vector machine method, and the coordinates of their central pixels were obtained. Then, nerve section images were registered using the bilinear method, and edges of fascicles were extracted using an improved gradient vector flow snake method. Subsequently, fascicle types were identified automatically using the multi-directional gradient and second-order gradient method. Finally, a 3D virtual model of internal fascicles was obtained after section images were processed. This technique was successfully applied for 3D reconstruction for the median nerve of the hand-wrist and cubital fossa regions and the gastrocnemius nerve. This nerve internal fascicle 3D reconstruction technology would be helpful for aiding peripheral nerve repair and virtual surgery.
Qi X.P.,Shenzhen Sixth Peoples Hospital |
Huang Y.Y.,Shenzhen Polytechnic |
Lin Z.S.,Shenzhen Institute for Drug Control |
Xu L.,Shenzhen Institute for Drug Control |
Yu H.,Shenzhen Polytechnic
Nanoscale Research Letters | Year: 2016
In the article, a dual-quantum-dots-labeled (dual-QDs-labeled) lateral flow strip (LFS) method was developed for the simultaneous and rapid quantitative detection of procalcitonin (PCT) and C-reactive protein (CRP) in the blood. Two QD-antibody conjugates with different fluorescence emission spectra were produced and sprayed on the LFS to capture PCT and CRP in the blood. Furthermore, a double antibody sandwich method for PCT and, meanwhile, a competitive inhibition method for CRP were employed in the LFS. For PCT and CRP in serum assayed by the dual-QDs-labeled LFS, their detection sensitivities reached 0.1 and 1 ng/mL, respectively, and their linear quantitative detection ranges were from 0.3 to 200 ng/mL and from 50 to 250 μg/mL, respectively. There was little evidence that the PCT and CRP assays would be interfered with each other. The correlations for testing CRP and PCT in clinical samples were 99.75 and 97.02 %, respectively, between the dual-QDs-labeled LFS we developed and commercial methods. The rapid quantification of PCT and CRP on dual-QDs-labeled LFS is of great clinical value to distinguish inflammation, bacterial infection, or viral infection and to provide guidance for the use of antibiotics or other medicines. © 2016, Qi et al.
PubMed | Southern Medical University, Shenzhen Sixth Peoples Hospital and People's Care
Type: Journal Article | Journal: Molecular medicine reports | Year: 2014
The herpes simplex virus thymidine kinase/ganciclovir (HSVTK/GCV) and the cytosine deaminase/5fluorocytosine (CD/5FC) systems have been widely applied in suicide gene therapy for cancer. Although suicide gene therapy has been successfully used in vitro and in vivo studies, the number of studies on the effects of recombinant adenoviruses (Ads) containing suicide genes on target cancer cells is limited. The aim of this study was to examine whether recombinant Ads containing the CD/TK fusion gene affect cell proliferation of breast cancer cells in vitro. In the present study, we explored the use of a recombinant adenoviral vector to deliver the CD/TK fusion gene to the breast cancer cell line MCF7. We found that the recombinant adenoviral vector efficiently infected MCF7 cells. Western blot analysis revealed that CD and TK proteins are expressed in the infected cells. The infected breast cancer cells did not show any significant changes in morphology, ultrastructure, cell growth, and cellcycle distribution compared to the uninfected cells. This study revealed that the Advascular endothelial growth factor promoter (VEGFp)CD/TK vector is nontoxic to MCF7 cells at the appropriate titer. Our results indicate that it is feasible to use a recombinant adenoviral vector containing the CD/TK fusion gene in suicide gene therapy to target breast cancer cells.
Chen H.,Sun Yat Sen University |
Chen H.,Guangdong Academy of Medical science |
Min X.-H.,Sun Yat Sen University |
Wang Q.-Y.,Guangdong Academy of Medical science |
And 8 more authors.
Scientific Reports | Year: 2015
Conditioned medium from mesenchymal stem cells (MSC-CM) may represent a promising alternative to MSCs transplantation, however, the low concentrations of growth factors in non-activated MSC-CM hamper its clinical application. Recent data indicated that the paracrine potential of MSCs could be enhanced by inflammatory factors. Herein, we pre-activated bone-marrow-derived MSCs under radiation-induced inflammatory condition (MSC IEC-6(IR)) and investigated the evidence and mechanism for the differential effects of MSC-CM IEC-6(IR) and non-activated MSC-CM on radiation-induced intestinal injury (RIII). Systemic infusion of MSC-CM IEC-6(IR), but not non-activated MSC-CM, dramatically improved intestinal damage and survival of irradiated rats. Such benefits may involve the modulation of epithelial regeneration and inflammation, as indicated by the regeneration of intestinal epithelial/stem cells, the regulation of the pro-/anti-inflammatory cytokine balance. The mechanism for the superior paracrine efficacy of MSC IEC-6(IR) is related to a higher secretion of regenerative, immunomodulatory and trafficking molecules, including the pivotal factor IGF-1, induced by TNF-α, IL-1β 2 and nitric oxide partially via a heme oxygenase-1 dependent mechanism. Together, our findings suggest that pre-activation of MSCs with TNF-α, IL-1β 2 and nitric oxide enhances its paracine effects on RIII via a heme oxygenase-1 dependent mechanism, which may help us to maximize the paracrine potential of MSCs.
Luo P.,Shenzhen Sixth Peoples Hospital |
Peng Q.-L.,Guangzhou Zhongda Medical Device Company |
Xiang J.-P.,Sun Yat Sen University |
Qi J.,Sun Yat Sen University
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: As the biodegradable materials produced nerve conduit can be degraded in vivo and can avoid the nerve entrapment, it has attracted more and more attentions. OBJECTIVE: To compare the effect of autogenous nerve transplantation and three kinds of synthetic biodegradable materials produced nerve conduit for the repair of peripheral nerve defects. METHODS: The effect of commonly used collagen nerve conduit, DL-lactic acid-ε-caprolactone nerve conduit, polyglycolic acid nerve conduit and autogenous nerve transplantation in the repair of peripheral nerve defects was evaluated with electrophysiological detection and morphological observation. RESULTS AND CONCLUSION: Theoretically, the nerve conduit has some advantages when compared with autogenous nerve transplantation, but there was significant difference in neural functional recovery between different synthetic materials produced nerve conduits. The repair effect of DL-lactic acid-ε-caprolactone nerve conduit was similar to that of autogenous nerve transplantation, and was considered as the ideal nerve conduit material; due to the disadvantages of polyglycolic acid nerve conduit which can inhibit its degradation, polyglycolic acid nerve conduit showed least effect in repairing peripheral nerve defects among three kinds of nerve conduits; collagen nerve conduit could improve the mechanical properties with the help of crosslinker, so the effect in repairing peripheral nerve defects was lower than DL-lactic acid-ε-caprolactone nerve conduit and higher than polyglycolic acid nerve conduit. These three kinds of nerve conduits have their potential shortcomings in nerve function regeneration, so that can not completely replace autogenous nerve transplantation. There still lacks of large sample long-term randomized controlled experiments to identify the performance price ratio between three kinds of nerve conduits. Further experimental observations are needed.
PubMed | Guangdong General Hospital and Guangdong Academy of Medical science, Shenzhen Sixth Peoples Hospital, Guangzhou University, University of California at Los Angeles and 3 more.
Type: | Journal: Scientific reports | Year: 2015
Conditioned medium from mesenchymal stem cells (MSC-CM) may represent a promising alternative to MSCs transplantation, however, the low concentrations of growth factors in non-activated MSC-CM hamper its clinical application. Recent data indicated that the paracrine potential of MSCs could be enhanced by inflammatory factors. Herein, we pre-activated bone-marrow-derived MSCs under radiation-induced inflammatory condition (MSC(IEC-6(IR))) and investigated the evidence and mechanism for the differential effects of MSC-CM(IEC-6(IR)) and non-activated MSC-CM on radiation-induced intestinal injury (RIII). Systemic infusion of MSC-CM(IEC-6(IR)), but not non-activated MSC-CM, dramatically improved intestinal damage and survival of irradiated rats. Such benefits may involve the modulation of epithelial regeneration and inflammation, as indicated by the regeneration of intestinal epithelial/stem cells, the regulation of the pro-/anti-inflammatory cytokine balance. The mechanism for the superior paracrine efficacy of MSC(IEC-6(IR)) is related to a higher secretion of regenerative, immunomodulatory and trafficking molecules, including the pivotal factor IGF-1, induced by TNF-, IL-1 and nitric oxide partially via a heme oxygenase-1 dependent mechanism. Together, our findings suggest that pre-activation of MSCs with TNF-, IL-1 and nitric oxide enhances its paracine effects on RIII via a heme oxygenase-1 dependent mechanism, which may help us to maximize the paracrine potential of MSCs.
Wang E.,Shenzhen Sixth Peoples Hospital |
Yi H.,Shenzhen Sixth Peoples Hospital |
Wang M.,Shenzhen Sixth Peoples Hospital |
Huang C.,Shenzhen Sixth Peoples Hospital
European Journal of Orthopaedic Surgery and Traumatology | Year: 2013
The incidence of osteoporotic vertebral compression fracture (OVCF) is increased recently. Percutaneous kyphoplasty (PKP) has recently been shown to have a curative effect on OVCF. Unfortunately, related complications arising from PKP cannot be ignored, such as cement leaks, fever, and intercostal neuralgia. This study aimed to investigate the common complications of PKP in the treatment of OVCF patients and analyze the causes and assess prevention and control measures. A total of 196 patients (204 vertebrae) underwent PKP procedures at the Department of Spine Surgery, Shenzhen Sixth People's Hospital, Guangdong, China, from June 2004 to August 2010. The data on incidence rates of the various complications were compiled. All patients were successfully operated without death, paraplegia, or pulmonary embolism. Incidence of various complications resulting from different types of bone cement leakage was 27.45%, including 0.51% for postoperative elevated fever, 4.08% for intercostal neuralgia, 2.55% for trailing of bone cement, 0.51% for refracture at adjacent vertebrae, and 0.51% for cerebrospinal fluid leakage. These results suggest that PKP is an effective surgical technique for the treatment of OVCF, resulting in limited trauma with satisfactory curative effects. Skillful mastery of this technology will help reduce the incidence rate of complications. © Springer-Verlag 2013.