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Luo P.,Shenzhen Sixth Peoples Hospital | Peng Q.-L.,Guangzhou Zhongda Medical Device Company | Xiang J.-P.,Sun Yat Sen University | Qi J.,Sun Yat Sen University
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: As the biodegradable materials produced nerve conduit can be degraded in vivo and can avoid the nerve entrapment, it has attracted more and more attentions. OBJECTIVE: To compare the effect of autogenous nerve transplantation and three kinds of synthetic biodegradable materials produced nerve conduit for the repair of peripheral nerve defects. METHODS: The effect of commonly used collagen nerve conduit, DL-lactic acid-ε-caprolactone nerve conduit, polyglycolic acid nerve conduit and autogenous nerve transplantation in the repair of peripheral nerve defects was evaluated with electrophysiological detection and morphological observation. RESULTS AND CONCLUSION: Theoretically, the nerve conduit has some advantages when compared with autogenous nerve transplantation, but there was significant difference in neural functional recovery between different synthetic materials produced nerve conduits. The repair effect of DL-lactic acid-ε-caprolactone nerve conduit was similar to that of autogenous nerve transplantation, and was considered as the ideal nerve conduit material; due to the disadvantages of polyglycolic acid nerve conduit which can inhibit its degradation, polyglycolic acid nerve conduit showed least effect in repairing peripheral nerve defects among three kinds of nerve conduits; collagen nerve conduit could improve the mechanical properties with the help of crosslinker, so the effect in repairing peripheral nerve defects was lower than DL-lactic acid-ε-caprolactone nerve conduit and higher than polyglycolic acid nerve conduit. These three kinds of nerve conduits have their potential shortcomings in nerve function regeneration, so that can not completely replace autogenous nerve transplantation. There still lacks of large sample long-term randomized controlled experiments to identify the performance price ratio between three kinds of nerve conduits. Further experimental observations are needed.

Qi X.P.,Shenzhen Sixth Peoples Hospital | Huang Y.Y.,Shenzhen Polytechnic | Lin Z.S.,Shenzhen Institute for Drug Control | Xu L.,Shenzhen Institute for Drug Control | Yu H.,Shenzhen Polytechnic
Nanoscale Research Letters | Year: 2016

In the article, a dual-quantum-dots-labeled (dual-QDs-labeled) lateral flow strip (LFS) method was developed for the simultaneous and rapid quantitative detection of procalcitonin (PCT) and C-reactive protein (CRP) in the blood. Two QD-antibody conjugates with different fluorescence emission spectra were produced and sprayed on the LFS to capture PCT and CRP in the blood. Furthermore, a double antibody sandwich method for PCT and, meanwhile, a competitive inhibition method for CRP were employed in the LFS. For PCT and CRP in serum assayed by the dual-QDs-labeled LFS, their detection sensitivities reached 0.1 and 1 ng/mL, respectively, and their linear quantitative detection ranges were from 0.3 to 200 ng/mL and from 50 to 250 μg/mL, respectively. There was little evidence that the PCT and CRP assays would be interfered with each other. The correlations for testing CRP and PCT in clinical samples were 99.75 and 97.02 %, respectively, between the dual-QDs-labeled LFS we developed and commercial methods. The rapid quantification of PCT and CRP on dual-QDs-labeled LFS is of great clinical value to distinguish inflammation, bacterial infection, or viral infection and to provide guidance for the use of antibiotics or other medicines. © 2016, Qi et al.

Ni Z.,Shenzhen University | Liu S.,Shenzhen University | Wang Y.,Shenzhen University | Liu X.,Shenzhen Sixth Peoples Hospital | Jiang Z.,Jilin University
Shenzhen Daxue Xuebao (Ligong Ban)/Journal of Shenzhen University Science and Engineering | Year: 2013

On the basis of preparing polyetheretherketone-hydroxyapatite (PEEK-HA) composite materials, this paper focuses on the effect of the composite materials on 3T3 cells proliferation at different periods and under different concentrations. Based on the polycondensation of polyether-ether-ketone, the biological material was prepared by adding 10%, 20% and 30% hydroxyapatite blending into PEEK, respectively. The cell morphology 3T3 cell adhesion rate of the material was determined by cell counting with inverted fluorescence microscope. The MTT (4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) method was used to study cell proliferation in various conditions. Results show that, in a concentration of 4 mg/mL, the material played a significant role in cell proliferation, and, in a concentration of 64 mg/mL, the effect of PEEK composites on cell proliferation is slight. In addition, cell adhesion rate increases with an increase of the HA content in PEEK-HA materials. The content of HA can improve cell adhesion to the composite materials. Cytotoxicity of the materials is found at levelI, indicating that PEEK-HA composites are non-toxic to 3T3 cells.

Chen H.,Sun Yat Sen University | Chen H.,Guangdong Academy of Medical science | Min X.-H.,Sun Yat Sen University | Wang Q.-Y.,Guangdong Academy of Medical science | And 8 more authors.
Scientific Reports | Year: 2015

Conditioned medium from mesenchymal stem cells (MSC-CM) may represent a promising alternative to MSCs transplantation, however, the low concentrations of growth factors in non-activated MSC-CM hamper its clinical application. Recent data indicated that the paracrine potential of MSCs could be enhanced by inflammatory factors. Herein, we pre-activated bone-marrow-derived MSCs under radiation-induced inflammatory condition (MSC IEC-6(IR)) and investigated the evidence and mechanism for the differential effects of MSC-CM IEC-6(IR) and non-activated MSC-CM on radiation-induced intestinal injury (RIII). Systemic infusion of MSC-CM IEC-6(IR), but not non-activated MSC-CM, dramatically improved intestinal damage and survival of irradiated rats. Such benefits may involve the modulation of epithelial regeneration and inflammation, as indicated by the regeneration of intestinal epithelial/stem cells, the regulation of the pro-/anti-inflammatory cytokine balance. The mechanism for the superior paracrine efficacy of MSC IEC-6(IR) is related to a higher secretion of regenerative, immunomodulatory and trafficking molecules, including the pivotal factor IGF-1, induced by TNF-α, IL-1β 2 and nitric oxide partially via a heme oxygenase-1 dependent mechanism. Together, our findings suggest that pre-activation of MSCs with TNF-α, IL-1β 2 and nitric oxide enhances its paracine effects on RIII via a heme oxygenase-1 dependent mechanism, which may help us to maximize the paracrine potential of MSCs.

Xue L.-Q.,Shanghai JiaoTong University | Han B.,Shanghai JiaoTong University | Chen L.-B.,Shenzhen Sixth Peoples Hospital | Pan C.-M.,Shanghai JiaoTong University | And 8 more authors.
Translational Research | Year: 2013

17α-hydroxylase/17,20-lyase deficiency (17OHD) is a rare autosomal recessive genetic disease that is characterized by low-renin hypertension, hypokalemia, and abnormal development of the genitalia. Mutations in the CYP17A1 gene account for this disease. We aim to investigate the CYP17A1 mutation and analyze its possible influence on phenotype in a Chinese patient with 17OHD. Steroid hormones were assayed. The 8 exons of the CYP17A1 gene were amplified and directly sequenced. Wild-type and mutant CYP17A1 cDNA were cloned into pcDNA3.1 expression vectors and transfected into 293T cells. Finally, 17-hydroxylase and 17,20-lyase activity were detected by using progesterone and 17-hydroxypregnenolone as the substrates. A novel missense mutation c.716 G>A located in exon 4 that changed the amino acid from arginine to glutamine (R239Q) was discovered in the patient. Steric model analysis of CYP17A1 showed that R239Q changed the local structure and the electrostatic potential. Functional study indicated that the R239Q mutant caused the complete loss of both 17α-hydroxylase and 17,20-lyase activities. Our study expanded the CYP17A1 mutation spectrum. With a functional study, we confirmed that the novel mutation caused the complete loss of both 17α-hydroxylase and 17,20-lyase activities. © 2013 Mosby, Inc. All rights reserved.

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