Shenzhen Prevention and Treatment Center for Occupational Disease

Shenzhen, China

Shenzhen Prevention and Treatment Center for Occupational Disease

Shenzhen, China

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Hui C.-Y.,Shenzhen Prevention and Treatment Center for Occupational Disease | Xie X.-B.,Hunan University | Cao H.,Southern Medical University | Huang S.-H.,Saban Research Institute
Anti-Infective Agents | Year: 2013

Continual genetic variation supports hepatitis C virus (HCV) persistent infection, a leading cause of cirrhosis and hepatocellular carcinoma. The current standard of care has limited efficacy and is associated with severe adverse effects. Efforts to improve patients' outcomes have focused on the design and development of small molecule compounds targeted towards essential viral proteins. Following the clinical success of HIV protease inhibitors, the HCV NS3-4A protease domain emerged as one of the most successful antiviral drug targets. This mini-review describes several HCV protease inhibitors in the various stages of clinical trials, and discusses their antiviral activities, pharmacokinetic properties, side effects and resistance profiles. © 2013 Bentham Science Publishers.


Li S.F.,Shenzhen Prevention and Treatment Center for Occupational Disease
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2011

To develop a method to determine phosphoric acid in the air of workplace by ion chromatography. Phosphoric acid was collected by millipore filter and washed by deionized water then detected by ion chromatography. Linearity range of test was 0 ∼ 20 μg/ml, relative standard deviation (RSD) was 1.95% ∼ 3.31%, the elution efficiency was 103.0% ∼ 109.6%, determination limit was 0.1 μg/ml (when sample size was 20.01) concentration limit was 0.01 mg/m(3) (when the collected air was 75 L). This method is convenient for air collection, simple, with high sensitivity and good precision, is a good method for determination of phosphoric acid in the air of workplace.


Hui C.-Y.,Shenzhen Prevention and Treatment Center for Occupational Disease | Guo Y.,CAS Shenzhen Institutes of Advanced Technology | Zhang X.,CAS Shanghai Institute of Materia Medica | Shao J.-H.,Yangzhou University | And 2 more authors.
Biologicals | Year: 2013

Defibrotide is a single-stranded nucleic acid polymer originally derived from porcine mucosa. Cheap salmon sperm DNA is commercially available and widely used in drug production. In this study, oligodeoxyribonucleotides were successfully obtained from the controlled depolymerization of salmon sperm DNA. The obtained product shared similar chemical and biological properties with defibrotide produced by Gentium SpA, Italy. It was also found that oligodeoxyribonucleotides derived from non-mammalian origins could also directly stimulate tissue plasminogen activator (t-PA) release from cultured human endothelial cells, and enhance fibrinolytic activity in the rabbit. © 2013 The International Alliance for Biological Standardization.


Shao J.,Yangzhou University | Zhao C.,Yangzhou University | Hui C.,Shenzhen Prevention And Treatment Center For Occupational Disease | Zhao R.,Yangzhou University | Ruan X.,Health Science Research
Turkish Journal of Biochemistry | Year: 2013

Aim: To clone genes encoding analgesic peptides from the cDNA pool of scorpion telson, to obtain recombinant peptides by prokaryotic expression system and examine their analgesic activity in mouse twisting test. Material and methods: The genes encoding analgesic peptides were cloned from the cDNA pool of scorpion telson by nested PCR. Positive clones were sequenced after screened by PCR-SSCP. The recombinant peptides were obtained by functionally expression in E. coli and purified by metal chelating chromatography. The bioactivity was assayed in mouse twisting test. Results: Two nucleotide sequences encoding potential analgesic peptides were obtained. They were named as BmK 22 and BmK 9. BmK 22 was a new peptide with only one amino acid at site 54 different with BmK 9. In mouse-twisting test, both of the two recombinant peptides exhibited analgesic activity, and BmK 9 showed a stronger activity in pain relieving. Further, when considering structure factors by homology modeling, we speculated that the Arg residue at site 54 of BmK 9 may play an important role in target recognition and influence the analgesic activity. Conclusion: Venoms from scorpions contain extremely rich bioactive peptides. The strategy in this paper involving molecular cloning, functional expression and bioactivity identification of BmK 9 and BmK 22 provided a rapid route to discover scorpion toxins with special bioactivity such as analgesics.


PubMed | Shenzhen Prevention and Treatment Center for Occupational Disease
Type: Journal Article | Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases | Year: 2016

To investigate the prevalence of metabolic syndrome (MS)in 259 professional automobile drivers, and to put forward targeted suggestions on protection.In October 2014, 114 male bus drivers and 145 male taxi drivers in a transportation service company were enrolled as investigation group, and 121 non-operating male staff were enrolled as control group. Physical examination and a questionnaire survey were conducted for both groups, and the results were analyzed.The bus drivers and taxi drivers had significantly higher prevalence rates of MS than the nonoperating staff(17.5%/13.1% vs 3.3%, P<0.05). The results of univariate logistic analysis showed that smoking(OR=2.58, 95%CI 1.14~5.88), exercise (OR=0.21, 95% CI 0.10~0.43), meal time (OR=0.27, 95% CI 0.13~0.59), and a family history of chronic diseases (OR=2.26, 95% CI 1.13~4.50)were associated with MS, and each independent variable showed significant differences between groups (P<0.05). Multivariate logistic regression analysis showed that with age remaining the same, smoking was the risk factor for MS in professional automobile drivers (OR=5.25, 95%CI 2.00~13.80), and meal time (20~40 min)(OR= 0.20, 95%CI 0.09~0.44)and exercise (OR=0.13, 95% CI 0.06~0.30)were protective factors against MS.Professional automobile drivers have a higher prevalence rate of MS than non-operating staff, which should be taken seriously by working personnel.


PubMed | Shenzhen Prevention and Treatment Center for Occupational Disease, Guangzhou University, Southern Medical University and University of Southern California
Type: Journal Article | Journal: Pathogens and disease | Year: 2015

OmpT is one of the members of the outer membrane protein family that has been identified as a virulence factor in most of the uropathogenic Escherichia coli (UPEC). However, the exact role of OmpT in the urinary tract infections (UTIs) remains unclear. To determine the role of OmpT in the pathogenesis of UPEC, an isogenic deletion mutant of ompT (COTD) was constructed by the Red recombination. Human bladder epithelial cell line 5637(HBEC 5637) was used to evaluate the ability of bacterial adhesion/invasion. A murine model of UTI was established to study the formation of intracellular bacterial communities (IBCs) in the process of UTIs. The cytokines were also examined during the pathogenesis. The results showed that the COTD strain was deficient in bacterial adhesion and invasion as well as in IBC formation compare to the parent strain. ELISA quantification analysis of cytokines showed that the levels of TNF-, IL-6 and IL-8 in the serum, bladder and kidney tissues of the mice infected with COTD were lower than that of the CFT073 group. In summary, these results suggest that OmpT plays a multifaceted role in pathogenesis of UTI, including increased bacterial adhesiveness/invasiveness, formation of IBCs and upregulated proinflammatory cytokines.


Linqing Y.,Centers for Disease Control and Prevention | Bo X.,Centers for Disease Control and Prevention | Xueqin Y.,Shenzhen Prevention and Treatment Center for Occupational Disease | Hong D.,Shenzhen Longgang District Center for Disease Control and Prevention | And 5 more authors.
Toxicology Letters | Year: 2016

A matched case-control study was conducted to examine the relationship between chromium (Cr) exposure and variation in mitochondrial (mt) DNA methylation. We enrolled 29 pairs of subjects in this study; Cr exposure was confirmed in the cases by detecting blood Cr and other metal ion concentrations. DNA damage caused by Cr exposure was determined in terms of binucleated micronucleus frequency (BNMN) and mtDNA copy number. Finally, a Sequenom MassARRAY platform was applied to inspect the DNA methylation levels of mitochondrially encoded tRNA phenylalanine (MT-TF), mitochondrially encoded 12S RNA (MT-RNR1), and long interspersed nucleotide element-1 (LINE-1) genes. The blood Cr ion concentration and micronucleus frequency of the Cr-exposed group were higher than those of the control group, whereas the mtDNA copy number remained unchanged. The methylation levels of MT-TF and MT-RNR1 but not LINE-1 were significantly lower in Cr-exposed workers. Pearson correlation analysis showed that workers with higher blood Cr ion concentrations exhibited lower MT-TF and MT-RNR1 gene methylation, and multiple linear regression analysis indicated that CpG sites 1 and 2 in MT-TF and CpG site 6 in MT-RNR1 were affected. These results suggested that methylation level of mtDNA has the possibility of acting as an alternative effect biomarker for Cr exposure. © 2015.


Yu W.,Centers for Disease Control and Prevention | Yu I.T.S.,Chinese University of Hong Kong | Li Z.,Shenzhen Prevention and Treatment Center for Occupational Disease | Wang X.,Chinese University of Hong Kong | And 5 more authors.
Accident Analysis and Prevention | Year: 2012

This study describes the frequency of occurrence of work-related injuries and musculoskeletal disorders (MSDs) among factory workers in Shenzhen, China, and examines the determinants of injuries and MSDs. A cross-sectional survey was conducted with 3479 frontline workers in 60 factories during 2008-2009. We calculated the annual incidence rates of work-related injuries and the prevalence rates of MSD (based on the Nordic Standard Form) for the factory workers. Logistic regression analysis was applied to explore the associations between potential risk factors and occupational injury and MSD. Among all participating workers, 8.3% reported acute traumatic injuries in the previous 12 months. The event-based incidence rate was 119.6 per 1000 person-years. About half of the frontline workers reported suffering from MSDs during the previous 12 months. The injury risk was reduced for female workers (Odds ratio, OR: 0.48; 95% confidence interval, CI: 0.36-0.66) and those with higher educational levels, but increased in workers with over 55 work hours/week (OR: 1.64; 95% CI: 1.21-2.22), high mental stress at work (OR: 3.50; 95% CI: 1.70-7.19) and previous injury history (OR: 3.94; 95% CI: 2.78-5.58). The MSD risk was increased among female workers (OR: 1.58; 95% CI: 1.34-1.87), and those with over 55 work hours/week (OR: 1.56; 95% CI: 1.28-1.90), high mental stress at work (OR: 3.16; 95% CI: 2.04-4.89) and a past injury history (OR: 3.04; 95% CI: 2.14-4.32). High proportions of frontline workers were affected by work injuries and MSDs in these factories in China. Long work hours, high mental stress at work and previous injury history were important risk factors for work-related injury and MSD. © 2012 Elsevier Ltd. All rights reserved.


Yu W.,Centers for Disease Control and Prevention | Yu I.T.S.,Chinese University of Hong Kong | Wang X.,Chinese University of Hong Kong | Li Z.,Shenzhen Prevention and Treatment Center for Occupational Disease | And 5 more authors.
International Archives of Occupational and Environmental Health | Year: 2013

Purpose Health and safety training program has been applied to prevent work-related musculoskeletal disorders (MSDs) in workplace. We evaluated the effectiveness of participatory training and didactic training programs on MSD prevention among frontline workers in Shenzhen, China. Methods The authors randomly assigned 918 workers from intervention factories to receive participatory training (intervention group), and 907 workers from intervention factories and 1,654 workers from control factories to receive didactic training (control-1 group, control-2 group, respectively) from June 1, 2008 to November 30, 2009. Participants were asked to reportexperience of ache, pain or discomfort in 10 body parts at baseline and 1 year aftertraining. Data were analyzed to compare the MSD prevalence 1 year before and 1 year after training in different groups from 2009 to 2010. Results The follow-up rate was 61 % (2,120/3,479) at1 year after training. In the year after training, there were no statistically significant changes in the proportion of workers who reported MSD in any body part. MSD prevalence rates in the intervention group reduced from 16.8 to 9.9 % for lower extremities (X2=13.102, p\0.001) and from 12.9 to 8.3 % (X2= 9.433, p = 0.002) for wrist and finger at 1 year after training. However, the rates did not change significantly for upper back, lower back, neck, shoulder and elbow in the intervention group and for all 10 body parts in two control groups. Conclusions Overall, the training programs did not seem to prevent the occurrence of MSD among frontline workers. However, participatory training might be effective to reduce MSD in the lower extremities and wrist and finger. © Springer-Verlag 2012.


Weng S.,Huazhong University of Science and Technology | Weng S.,Shenzhen Prevention and Treatment Center for Occupational Disease | Wang L.,Huazhong University of Science and Technology | Wang L.,Centers for Disease Control and Prevention | And 5 more authors.
PLoS ONE | Year: 2015

Objectives To evaluate the effects of the interactions between polymorphisms in Nalp3, caspase-1, and interleukin(IL)-1β genes and occupational dust exposure on the risk of silicosis. Methods We conducted a population-based case-control study in a large iron mine in China. Between January 2006 and December 2009, we identified 179 patients with silicosis to evaluate as cases and 201 individuals without silicosis to evaluate as controls. We estimated cumulative dust exposure (CDE) for all subjects and we genotyped polymorphisms in Nalp3, caspase-1, and IL-1β genes. We estimated odds ratios(ORs), 95% confidence intervals(95%CIs), and p-values using logistic regression models adjusted for selected confounders. Results After adjusting for age, smoking status, and CDE, subjects with the CT genotype of Ex4-849C>T in Nalp3 and the GA genotype of Ex2+37G>A in caspase-1 had increased risks of silicosis (adjusted ORs[95%CIs] = 2.40 [1.12-5.12] and 3.62 [1.63-8.02], respectively). Among subjects younger than 70 years old, those with the CC genotype of IVS8-7652A>C inNalp3 had a lower risk of silicosis than those with other genotypes (adjusted OR[95%CI] = 0.24 [0.06-0.88]). Among subjects aged 70 years and older, those with the CT genotype of Ex4-849C>T in Nalp3 and those with the GA genotype of Ex2+37G>A in caspase-1 had a higher risk of silicosis than those with other genotypes (adjusted ORs [95%CI] = 2.52[1.04-6.12] and 5.19[1.88-14.35], respectively). Among subjects with CDE greater than 120 mg/m3×year and among smokers, those with the GA genotype of Ex2+37G>A in caspase-1 had a higher risk of silicosis than those with other genotypes (adjusted ORs[95%CIs] = 26.37[3.35-207.39] and 3.47[1.40-8.64], respectively). Conclusions Genetic polymorphisms in Nalp3 and caspase-1 may be associated with individual susceptibility to silicosis, especially when the polymorphisms interact with age, CDE, or smoking status. © 2015 Weng et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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