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Shenzhen, China

Yan C.,CAS Institute of Process Engineering | Zhang Q.,CAS Institute of Process Engineering | Xue S.,CAS Institute of Process Engineering | Sun Z.,University of Chinese Academy of Sciences | And 4 more authors.
Biotechnology and Bioprocess Engineering | Year: 2016

In this study, a novel thin-film flat plate photobioreactor (FPPBR) mounted with baffles and a 61.2 m2 (2,000 L) photobioreactor system based on the FPPBR were developed. The flow of the fluid in the thinfilm photobioreactor was investigated by means of computational fluid dynamics (CFD). The cultivation of Chlorella sp. and Scenedesmus dimorphus in the thin-film FPPBR was carried out outdoors. The results showed that the flow of culture medium in different channels was uniform. In outdoor cultivation, the biomass productivity in the FPPBR with baffles was 25.2% higher than that in the FPPBR without baffles. In the pilot-scale FPPBR system, the maximum area productivity of Scenedesmus dimorphus reached 21.9 g/m2/day. When the service time of the photobioreactor was 1 and 3 years, the capital cost of the photobioreactor was 4.72 and 2.45 $ kgalgae, respectively. The results demonstrated that the thin-film FPPBR was cost effective, and it has the potential to be used for mass cultivation of microalgae. © 2016, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg. Source


Luan M.,Chinese Academy of Agricultural Sciences | Xu M.,Chinese Academy of Agricultural Sciences | Lu Y.,Shenzhen Nongke Group Co. | Zhang L.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Gene | Year: 2015

The miR169 miRNA family is highly conserved in plants. Its members regulate the expression of genes encoding the universal transcription factor subunit NUCLEAR FACTOR-Y subunit A (NF-YA) via transcript cleavage. NF-YA regulates gene expression by binding the CCAAT box sequence in target promoters. The miR169/NF-YA module plays a critical role during plant development and in plant responses to abiotic stress. We characterized the secondary structures of maize pre-miR169 miRNAs and predicted their potential gene targets. Coexpression of zma-miR169 and ZmNF-YA in Nicotiana benthamiana demonstrated that mutations in or deletion of target sites abolished regulation by zma-miR169. Maize seedlings were subjected to short-term (0-48. h) and long-term (15. days) drought, abscisic acid (ABA), or salt stress. Long-term exposure to PEG (drought stress) or NaCl (salt stress) repressed seedling growth. We investigated the expression patterns of zma-miR169s and their target ZmNF-YA genes in maize leaves and found diverse changes in expression in response to the three stress treatments. The expression of most zma-miR169 genes was downregulated by PEG and upregulated by ABA. In response to salt stress, zma-miR169 genes were upregulated initially and subsequently downregulated. Most ZmNF-YA genes were upregulated during the short term and downregulated by 15. days in response to the three stress treatments. © 2014 Elsevier B.V. Source


Zhou M.-L.,Chinese Academy of Agricultural Sciences | Zhou M.-L.,Leiden University | Ma J.-T.,Chinese Academy of Agricultural Sciences | Pang J.-F.,Chinese Academy of Agricultural Sciences | And 4 more authors.
African Journal of Biotechnology | Year: 2010

Plant growth and productivity are greatly affected by environmental stresses such as dehydration, high salinity, low temperature and biotic pathogen infection. Plant adaptation to these environmental stresses is controlled by cascades of molecular networks. The dehydration responsive element binding (DREB) transcription factors, which specifically interact with C-repeat/DRE (A/GCCGAC), play an important role in plant environmental stress tolerance by controlling the expression of many stress related genes. This review specifically focused on the structure characteristics of DREB proteins and their roles in regulating abiotic and biotic stress tolerance in plants. The DREB proteins are also involved in phytohormones signaling pathway such as abscisic acid, salicylic acid, jasmonate acid, ethylene and gibberellic acid. In addition, this review summarized the progress of the genetic engineering of DREB transcription factors in the main crops and model plants. © 2010 Academic Journals. Source


Xu M.,Chinese Academy of Agricultural Sciences | Lu Y.,Shenzhen Nongke Group Co. | Yang H.,Chinese Academy of Agricultural Sciences | Yang H.,Xinjiang Academy of Agricultural Science | And 10 more authors.
Plant Molecular Biology | Year: 2014

Transcription factors that act as positive regulators of gibberellin (GA) biosynthetic genes in plants are not well understood. A nuclear-localized basic leucine zipper transcription factor, ZmGRF, was isolated from maize. The core DNA sequence motif recognized for binding by ZmGRF was CCANNTGGC. ZmGRF overexpression in transgenic Arabidopsis plants promoted flowering, stem elongation, and cell expansion. Chromatin immunoprecipitation assays revealed that ZmGRF bound directly to the cis-element CCANNTGGC in the promoter of the Arabidopsisent-kaurene oxidase (AtKO1) gene and promoted AtKO1 expression. GA4 content increased by 372–567 % in transgenic Arabidopsis plants overexpressing ZmGRF compared to wild-type control plants. The GIBBERELLIN-INSENSITIVE DWARF1 gene, which encodes a GA receptor, was also upregulated and the growth-repressing DELLA protein gene GA INSENSITIVE was downregulated. Our results showed ZmGRF functioned through the GA-signaling pathway. © 2014 Springer Science+Business Media Dordrecht Source


Luan M.,Southwest University of Science and Technology | Luan M.,Chinese Academy of Agricultural Sciences | Xu M.,Chinese Academy of Agricultural Sciences | Lu Y.,Shenzhen Nongke Group Co. | And 8 more authors.
PLoS ONE | Year: 2014

Previous studies have identified miR169/NF-YA modules are important regulators of plant development and stress responses. Currently, reported genome sequence data offers an opportunity for global characterization of miR169 and NF-YA genes, which may provide insights into the molecular mechanisms of the miR169/NF-YA modules in maize. In our study, fourteen NF-YA transcription factors with conserved domains were identified based on maize genome loci. The miR169 gene family has 18 members that generate 10 mature products, and 8 of these mature miR169 members could target 7 of 14 ZmNF-YA genes in maize. The seven ZmNF-YA proteins were localized to the nucleus while lacked transcriptional activity. We investigated the expression patterns of the zma-miR169 members and their targeted ZmNF-YA genes in maize roots treated by drought stress (polyethylene glycol, PEG), hormone stress (abscisic acid, ABA), and salt stress (NaCl). The zma-miR169 family members were downregulated in short term (0-48 h) and generally upregulated over the long term (15 days) in response to the three abiotic stress conditions. Most of the targeted ZmNF-YA genes exhibited a reverse correlation with zma-miR169 gene expression over both the short term and long term. Maize root elongation was promoted by PEG and ABA but repressed by NaCl over the long term. Apparently, ZmNF-YA14 expression perfectly matched the zma-miR169 expression and corresponded to root growth reversely. © 2014 Luan et al. Source

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