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Ji P.,University of Sichuan | Jiang T.,Shenzhen Nanshan Hospital | Li Y.,University of Sichuan | Wang R.-R.,University of Sichuan
Journal of Sichuan University (Medical Science Edition) | Year: 2014

Objective To investigate the role of Capsaicin sensitive C fibers (CapsCF) denervation in lung ischemia-reperfusion (IR) injury and the possible mechanism related to oxidative stress. Methods Thirty two male New Zealand rabbits were randomized into four groups; IR group (IR), sham group (S), capsaicin pretreated IR group (CIR), and capsaicin pretreated sham group (CS). The rabbits in CIR and CS groups were pretreated with capsaicin (100mg/kg) to induce functional ablation of CapsCF, and then subjected to lung ischemia and reperfusion. The rabbits in IR group were not treated with capsaicin before lung ischemia and reperfusion. Thereafter, blood samples and lung tissue samples were obtained for blood gas and biochemical analyses, including the measurements of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT). The lung wet/dry weight ratio and histopathological changes were also assessed. Results Compared to S and CS group, partial pressure of oxygen (PO2) values in IR and CIR groups significantly decreased (P<0. 05). In contrast, the alveolar-arterial oxygen gradient (A-aDO2), lung wet/dry weight ratio increased in IR and CIR groups (P<0. 05). Capsaicin pretreatment in CIR group increased lung wet/dry weight ratio and lung pathologic lesions, along with higher level of MDA and lower activity of SOD and CAT (P<0.05, vs. IR). Conclusion Denervation of CapsCF agggravated lung ischemia-reperfusion injury of rabbits, which seems to be closly related to the excerbation of oxidative stress. Source


Yang L.,Huazhong University of Science and Technology | Ye S.,Huazhong University of Science and Technology | Kan C.,Huazhong University of Science and Technology | Yang J.,Huazhong University of Science and Technology | And 4 more authors.
Chinese Journal of Clinical Oncology | Year: 2011

Objective: To explore the expression and significance of Plexin-B2 in human breast cancer and to determine whether its co-expression with human epidermal growth factor receptor 2 (Her-2) is correlated with tumor aggressiveness. Methods: The protein expression of Plexin-B2 and Her-2 was investigated by immunohistochemistry SP method among 112 cases of breast carcinoma. The protein expression of Plexin-B2 was also detected among 15 cases of normal breast tissues. Results: There was no significant difference in the expression of Plexin-B2 between the breast carcinoma cells and the epithelial cells of the normal breast tissue from mammary lobules and ducts (P> 0.05); their expression rates were 69.64% and 60.00%, respectively. The positive expression of Plexin-B2 was detected in 44.62% of pericancerous microvessels in the breast cancer tissues, whereas no Plexin-B2 expression was observed in the microvessels of the normal breast tissues. There was a positive relationship in Plexin-B2 expression between the tumor cells and the pericancerous microvessels in human breast cancer (r = 0.593, P = 0.000), which were correlated with clinical stage and lymph node metastases (P < 0.05). Plexin-B2 expression in the tumor cells was also associated with the histologic grade (P < 0.05). The tumors that co-expressed Plexin-B2 and Her-2 were characterized by worse staging and higher incidences of lymph node metastases than those that express Plexin-B2 alone; the difference was significant (P < 0.05). Conclusion: The aberrant expression of Plexin-B2 may be associated with the malignant progression of breast cancer. Moreover, Plexin-B2 may contribute to the invasiveness and metastatic behavior of tumors when co-expressed with Her-2. Source


Wang Y.,Huazhong University of Science and Technology | Xiong L.,Huazhong University of Science and Technology | Yin X.,Hebei Medical University | Wang J.,First Hospital of Handan | And 6 more authors.
American Journal of Rhinology and Allergy | Year: 2014

Background: House dust mite (HDM) allergen is a risk factor for the development of allergic rhinitis (AR). Objectives: To determine the levels of indoor allergens in the households of patients with AR in Wuhan city, identify the environmental risk factors for high allergen exposure, and investigate the correlations between allergen exposure and specific immunoglobulin E levels and symptoms.Methods: The study examined 50 patients with AR. Two dust samples were collected from the bedding of each patient, one in summer and one in winter. Major allergens Der p 1 and Der f 1, from Dermatophagoides pteronyssinus and Dermatophagoides farinae, were measured with an enzyme-linked immunosorbent assay. Participants completed a standardized questionnaire about their living environments, and their rhinitis symptom scores were calculated. Specific immunoglobulin E levels against Der p and Der f were measured.Results: The percentage of bedding samples with high HDM allergen (Der f 1 + Der p 1) levels (>10 μg/g) was 44% in summer and 46% in winter. There was no significant difference between the level of mite allergens in summer and winter; however, the level of Der f 1 was higher than that of Der p 1 (p < 0.05). The age of the mattress and pillow was significantly correlated with allergen concentration. Indoor HDM allergen level affected the severity of nasal itching. Conclusions: HDMs are important indoor allergens in Wuhan. Mattresses and pillows that have been used for a long time contain high levels of allergens. High levels of exposure to HDM allergens correlates with the severity of nasal itching. © 2014, OceanSide Publications, Inc., U.S.A. Source


Xiao L.,Shenzhen No.6 Peoples Hospital | Cheng J.,Cleveland Clinic | Zhuang Y.,Huizhou Municipal Central Hospital | Qu W.,Mayo Medical School | And 3 more authors.
Pain Medicine (United States) | Year: 2013

Objective: We aim to determine the effects of botulinum toxin type A (BTX-A) on the thresholds of pain and the expression of transient receptor potential vanilloid type 1 (TRPV1) in the dorsal root ganglion (DRG) in rats with neuropathic pain induced by selective ventral root transection (VRT). Methods: Neuropathic pain was induced by transection of the lumbar 5 ventral root in male Sprague-Dawley rats. BTX-A or saline was administered to the plantar surface by subcutaneous injection. SB366791 (an inhibitor of TRPV1) was administered intraperitoneally. Behavioral tests were conducted preoperatively and at predefined postoperative days. The expression of TRPV1 was detected and quantified by immunohistochemistry and Western blotting at postoperative days 3, 7, 14, and 21. Results.: TRPV1 expression increased significantly in the L4 ∼5 dorsal root ganglia 7 days after L5 VRT compared with the sham-operated control (P<0.05). This increase persisted for at least 21 days. The thresholds of foot withdrawal to mechanical and thermal stimulation decreased significantly as well. Subcutaneous injection of BTX-A significantly and dose-dependently reduced the expression of TRPV1 (P<0.05) and partially reversed the pain thresholds. Conclusion: Upregulation of TRPV1 expression in the DRG is an important mechanism of neuropathic pain induced by the VRT. The analgesic effect of BTX-A is most likely mediated through reduction of TRPV1 expression in the nociceptors. Wiley Periodicals, Inc. Source


Bo X.-J.,Zunyi Medical College | Xu L.,Zunyi Medical College | Luo X.-D.,Shenzhen Nanshan Hospital | Liu F.-Y.,Shenzhen Nanshan Hospital | And 5 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: The low oxygen environment after femoral fracture and cerebral trauma will induce series of related cytokines expression, including hypoxia-inducible factor 1α and core binding factor α1, which play key roles in regulating bone healing. However, whether the accelerated bone healing is correlated with the expression of hypoxia-inducible factor 1α and core binding factor α1 is still unknown. OBJECTIVE: To construct rat models of brain injury, to compare the expression level of hypoxia-inducible factor 1α and core binding factor α1 in femoral fracture combined with cerebral trauma rats and simple femoral fracture rats, and to assess the influence of cerebral trauma on bone healing. METHODS: Rats were randomly divided into blank group, simple femoral fracture group and femoral fracture combined with cerebral trauma group. At 1, 2, 3 and 5 weeks after modeling, rats were executed. Bone healing was evaluated using femoral fracture end X-ray score and hematoxylin and eosin staining at callus tissues. Besides, the expression levels of hypoxia-inducible factor 1α and core binding factor α1 of three groups were determined with immunohistochemistry. RESULTS AND CONCLUSION: Bone healing in the femoral fracture combined with cerebral trauma group was better than that of simple femoral fracture group. There was significant difference in the expression level of hypoxia-inducible factor 1α and core binding factor α1 between the simple femoral fracture group and femoral fracture combined with cerebral trauma group (P < 0.05). At the same time, the level of simple femoral fracture group and femoral fracture combined with cerebral trauma group was significantly higher than that of blank group, and that in femoral fracture combined with cerebral trauma group was significantly higher than that of simple femoral fracture group (P < 0.05). Results verified that the expression levels of hypoxia-inducible factor 1α and core binding factor α1 of rats with femoral fracture combined with cerebral trauma were significantly high, which may be the major reason why the bone healing was accelerated after fracture combined with brain injury. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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