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Huining L.,Central South University | Jingting C.,Hunan Tumor Hospital | Keren H.,Shenzhen Maternity and Child Healthcare Hospital
European Journal of Gynaecological Oncology

Objective: Obtaining choriocarcinoma metastasis-associated genes and identifying the role and mechanism of VEGF-B in the progression of human choriocarcinoma. Study Design: (1) cDNA microarray technique was used to compare the transcriptional profiles between highly metastatic JEG-3 cells and lowly metastatic JAR cells; (2) An inhibitory effect of VEGF-B shRNA was demonstrat-ed by RT-PCR; (3) The effect of VEGF-B shRNA on invasion of JEG-3 cells in vitro was detected by Matrigel invasion assay. Results: (1) In upregulated genes, 51 genes were correlated with the cell metastasis ability, and FN, MMP-2, uPA, CAV-1 and VEGF-B were the first five genes; (2) Afterwards transfected VEGF-B shRNA, VEGF-B mRNA expression decreased obviously; (3) VEGF-B shRNA transfection significantly downregulated invasion level of JEG-3 cells in vitro (p < 0.05). Conclusion: VEGF-B plays an important role in the metastatic capability of human choriocarcinoma. Reducing the expression of VEGF-B can help weaken the invasion ability of human choriocarcinoma. Source

Fu Z.H.,Shenzhen Maternity and Child Healthcare Hospital
Nan fang yi ke da xue xue bao = Journal of Southern Medical University

To investigate the relationship between the expression of uncoupling protein 2 (UCP2) and ratio of interleukin-10/interferon-gamma (IL10/IFNgamma) in the macrophages from patients with unexplained recurrent spontaneous abortion (URSA). Twelve women undergoing selective termination of normal early pregnancy (control) and 11 having URSA were included in this study. Magnetic cell sorting (MACS) was used to isolate the macrophages in the decidua, and the expression of UCP2 was detected with flow cytometry. Cytokine (IL10 and IFNgamma) secretion by the macrophages was detected by enzyme-linked immunosorbent spot-forming (ELISPOT) cell assay. Compared to the control group, the women with URSA showed significantly decreased expression of UCP2 on decidual macrophage (136-/+25 vs 201-/+31, P<0.01), and the expression of UCP2 was positively correlated to the ratio of IL10/IFNgamma(r=0.73, P<0.01). UCP2 may play an important role in the regulation of macrophage activity and cytokine secretion to contribute to spontaneous abortion. Source

Fu Z.H.,Shenzhen Maternity and Child Healthcare Hospital
Zhonghua nan ke xue = National journal of andrology

OBJECTIVE: Germ cells are much more susceptible to oxidative stress than somatic cells. Accumulating evidence indicates that uncoupling protein 2 (UCP2) prevents the apoptosis of multiple types of cells induced by reactive oxygen species (ROS). The present study aimed to evaluate the protective effect of uncoupling protein 2 (UCP2) against oxidative damage to human sperm. METHODS: Semen parameters were obtained from 97 semen samples by computer assisted sperm analysis (CASA), and the samples, based on the percentage of grade a + b sperm, allotted to Groups I (n = 25, grade a + b sperm > 50%), II (n = 24, grade a + b sperm 25%-50%), III (n = 24, grade a + b sperm 10-25%) and IV (n = 24, grade a + b sperm < 10%). The expressions of sperm UCP2 mRNA was detected by RT-PCR, and the malonaldehyde (MDA) content in ROS was determined in the sperm suspension. RESULTS: The expressions of UCP2 mRNA in sperm were 1.51 +/- 0.24, 1.28 +/- 0.15, 1.17 +/- 0.20 and 0.69 +/- 0.18, and the MDA contents were (14.66 +/- 2.55), (16.00 +/- 2.09), (17.44 +/- 1.40) and (21.20 +/- 3.50) nmol/10(8) sperm in Groups I, II, III and lV, respectively. There was a significant negative correlation between the expression of UCP2 mRNA and the content of MDA (r = -0.633, P < 0.01), the former obviously reduced while the latter remarkably increased in the groups of lower sperm motility (P < 0.01). CONCLUSION: UCP2 plays an important protective role against oxidative stress damage to human sperm by diminishing ROS production. Source

Sheng C.,Southern Medical University | Yu Y.-H.,Southern Medical University | Zhao K.-S.,Southern Medical University | Huang L.-P.,Southern Medical University | And 5 more authors.
Critical Care Medicine

Objective: The acute respiratory distress syndrome may complicate postpartum hemorrhagic shock and resuscitation, but its mechanisms are not yet well defined. We studied the lung inflammatory response to postpartum hemorrhagic shock and resuscitation in a rabbit model and the role of the nuclear factor-κB pathway. Design: Randomized, controlled, prospective study. Setting: University hospital laboratory. Subjects: Nonobstetric (not pregnant nor postpartum) and obstetrical (within 2 hrs postpartum) rabbits. INTERVENTIONS:: Nonobstetric and obstetric female New Zealand white rabbits underwent fixed-pressure or fixed-volume hemorrhagic shock for 30 mins and then were rapidly resuscitated with the shed blood and Ringer's solution. Finally, they were either monitored for survival time or euthanized by exsanguination for lung tissue examination 24 hrs after hemorrhage. Measurements and Main Results: After hemorrhagic shock and resuscitation, median survival time in obstetric rabbits (3 days) was significantly shorter (p < .05) than that in nonobstetric rabbits (5 days). Compared with nonobstetric rabbits, obstetric rabbits had more severe lung injury as indicated by alveolar and interstitial fluid accumulation and marked neutrophil sequestration and greater lung injury score, myeloperoxidase activity, expression of intercellular adhesion molecule-1, serum tumor necrosis factor-α levels, and nuclear factor-κB activation, and lower serum interleukin-10 levels (p < .05 for all). Conclusions: After hemorrhage and resuscitation, obstetric rabbits had significantly shorter survival time and more severe lung injury than nonobstetric rabbits. The mechanism may be through upregulation of the signal transductions of the nuclear factor-κB pathways. © 2012 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins. Source

Gong S.-P.,Southern Medical University | Guo H.-X.,Shenzhen Maternity and Child Healthcare Hospital | Zhou H.-Z.,Southern Medical University | Chen L.,Southern Medical University | Yu Y.-H.,Southern Medical University
Journal of Obstetrics and Gynaecology Research

Aim: To estimate the incidence of and identify the risk factors for a surgical site infection after a cesarean section. Method: A survey of women who underwent a cesarean section was conducted in eight hospitals in Guangdong Province, China. The rate of surgical site infectionwas estimated and a nested case control studywas then carried out to identify the risk factors. Results: Among 13 798 women surveyed, 96 (0.7%) developed a surgical site infection after a cesarean section. Multivariate logistic regression analysis identified six factors independently associated with an increased risk of surgical site infection, which included obesity, premature rupture of membranes, lower preoperative hemoglobin, prolonged surgery, lack of prophylactic antibiotics and excessive anal examinations performed during hospitalization. Conclusion: Surgical site infection occurs in approximately 0.7% of cesarean section cases in the general obstetric population in China. Obesity, premature rupture of membranes, lower preoperative hemoglobin, prolonged surgery, lack of prophylactic antibiotics and excessive anal examinations during hospitalization are considered to be independent risk factors. © 2012 Japan Society of Obstetrics and Gynecology. Source

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