Zhang H.-J.,Shenzhen Main Luck Pharmaceuticals Inc. |
Xiang G.,Shenzhen Main Luck Pharmaceuticals Inc. |
Li R.-Z.,Shenzhen Main Luck Pharmaceuticals Inc. |
Wu Z.-X.,Shenzhen Main Luck Pharmaceuticals Inc. |
Yu Y.-G.,Shenzhen Main Luck Pharmaceuticals Inc.
Chinese Journal of Biologicals | Year: 2015
Objective: To optimize the culture medium for production of monoclonal antibody (McAb) against human epidermal growth factor receptor 2 (HEK2) by using design of experiments (DOF) so as to increase the expression level of McAb. Methods: The effects of various basal media and feeding media on cell density and expression of McAb against HKK2 in engineering cell were investigated by DOE with Minitab software. Results: After optimization by DOE, the viable cell density of recombinant cell strain reached 296 × 105 cells/ml at most. However, the expression level of antibody titer reached 2.07 g/L at most, which increased by 72% compared with that before optimization (1.20 g/L). The quality of antibody was similar to that of antibody developed previously as control. Conclusion: An optimized combination of basal media and feeding media was obtained, by which the cell density and antibody expression level increased. The optimized combination was suitable for production of McAb against HER2, indicating DOE as an effective method for rapidly increasing the expression level of antibody within a short time period. Source