Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety

Shenzhen, China

Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety

Shenzhen, China

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Shi Y.,Sun Yat Sen University | Zhang H.,Sun Yat Sen University | Zhang H.,Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety | Yue Z.,Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety | And 5 more authors.
Nanotechnology | Year: 2013

Advances in the controlled assembly of nanoscale building blocks have resulted in functional devices which can find applications in electronics, biomedical imaging, drug delivery etc. In this study, novel covalent nanohybrid materials based upon [Ru(bpy)3]2+-doped silica nanoparticles (SiNPs) and gold nanoparticles (AuNPs), which could be conditioned as OFF-ON probes for glutathione (GSH) detection, were designed and assembled in sequence, with the disulfide bonds as the bridging elements. The structural and optical properties of the nanohybrid architectures were characterized using transmission electron microscopy, UV-vis spectroscopy and fluorescence spectroscopy, respectively. Zeta potential measurements, x-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy were employed to monitor the reaction processes of the SiNPs-S-S-COOH and SiNPs-S-S-AuNPs synthesis. It was found that the covalent nanohybrid architectures were fluorescently dark (OFF state), indicating that SiNPs were effectively quenched by AuNPs. The fluorescence of the OFF-ON probe was resumed (ON state) when the bridge of the disulfide bond was cleaved by reducing reagents such as GSH. This work provides a new platform and strategy for GSH detection using covalent nanohybrid materials. © 2013 IOP Publishing Ltd.


Zhang H.,Sun Yat Sen University | Zhang H.,Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety | Lan F.,Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety | Shi Y.,Sun Yat Sen University | And 8 more authors.
Food Chemistry | Year: 2014

VitaFast® test kits designed for the microbiological assay in microtiter plate format can be applied to quantitative determination of B-group water-soluble vitamins such as vitamin B12, folic acid and biotin, et al. Compared to traditional microbiological methods, VitaFast® kits significantly reduce sample processing time and provide greater reliability, higher productivity and better accuracy. Recently, simultaneous determination of vitamin B12, folic acid and biotin in one sample is urgently required when evaluating the quality of infant formulae in our practical work. However, the present sample preparation protocols which are developed for individual test systems, are incompatible with simultaneous determination of several analytes. To solve this problem, a novel "three-in-one" sample preparation method is herein developed for simultaneous determination of B-group water-soluble vitamins using VitaFast® kits. The performance of this novel "three-in-one" sample preparation method was systematically evaluated through comparing with individual sample preparation protocols. The experimental results of the assays which employed "three-in-one" sample preparation method were in good agreement with those obtained from conventional VitaFast® extraction methods, indicating that the proposed "three-in-one" sample preparation method is applicable to the present three VitaFast® vitamin test systems, thus offering a promising alternative for the three independent sample preparation methods. The proposed new sample preparation method will significantly improve the efficiency of infant formulae inspection. © 2013 Elsevier Masson SAS. All rights reserved.


Zhang H.,Sun Yat Sen University | Zhang H.,Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety | Shi Y.,Sun Yat Sen University | Lan F.,Shenzhen Key Research Laboratory of Detection Technology RandD on Food Safety | And 6 more authors.
Chemical Communications | Year: 2014

A carbon nanotube (CNT)-based multiple cycle signal amplification strategy has been demonstrated for detection of single-digit foodborne pathogens with the naked eye. In the present design, CNTs are used as carriers for loading numerous horseradish peroxidase (HRP) and concanavalin A (ConA) tags, and multiple cycle signal amplification is achieved through the biotinylated anti-HRP antibody and avidin-HRP. © 2014 The Royal Society of Chemistry.

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