Feng J.J.,Animal and Plant Inspection and Quarantine Technology Center |
Feng J.J.,China Agricultural University |
Feng J.J.,Shenzhen Key Laboratory of Inspection Research and Development of Alien Pests |
Li J.Q.,China Agricultural University |
And 9 more authors.
Seed Science and Technology | Year: 2013
Acidovorax citrulli, the causal agent of a bacterial seedling blight and fruit blotch (BFB), has received considerable attention since its first appearance in commercial watermelon production areas in the United States 89. Subsequently, it has emerged as a serious pathogen of cucurbitaceous plant species worldwide. In the major cucurbit producing countries of the world, great emphasis has been placed on managing this potentially devastating disease. Thus far, the most effective strategy for BFB management has been exclusion, which relies heavily on rapid and reliable pathogen detection. This review summarises recent progress in the development of A. citrulli detection techniques and evaluates their effectiveness as it pertains to BFB management.
Zheng Y.,Shenzhen Key Laboratory of Inspection Research and Development of Alien Pests |
Zhang W.,Shenzhen Key Laboratory of Inspection Research and Development of Alien Pests |
Lu X.,Shenzhen Key Laboratory of Inspection Research and Development of Alien Pests |
Zhang G.,Shenzhen Key Laboratory of Inspection Research and Development of Alien Pests |
And 4 more authors.
Journal of Phytopathology | Year: 2013
Lily symptomless virus (LSV) and Arabis mosaic virus (ArMV) cause severe losses of quantity and quality of lily flower and bulb production. Specificity, sensitivity and speed of detection methods for viruses need to be improved greatly to prevent LSV and ArMV from spreading from infected lilies. A dual IC-RT-PCR procedure for detection was developed in which the antibodies of LSV and ArMV were mixed and the mixture used to coat the PCR tubes. The particles of the two viruses were captured by the respective antibodies. Interference by other RNA viruses in infected lily was eliminated in the RT-PCR. Also, an RNA extraction step was omitted. The dual IC-RT-PCR products of LSV and ArMV were 521 bp and 691 bp, respectively. The specificity of the method was validated; only LSV and ArMV of four viruses were detected by dual IC-RT-PCR. The sensitivity of the detection method is 1 mg leaf tissue and higher than DAS-ELISA due to enrichment by dual immunocapture. © 2013 Blackwell Verlag GmbH.