Shenzhen Key Laboratory of Detection Technology for Food Safety

Shenzhen, China

Shenzhen Key Laboratory of Detection Technology for Food Safety

Shenzhen, China
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Chen B.,Sun Yat Sen University | Chen B.,Shenzhen Key Laboratory of Detection Technology for Food Safety | Jin B.,Shenzhen Key Laboratory of Detection Technology for Food Safety | Jiang R.,Sun Yat Sen University | And 4 more authors.
Analytical Methods | Year: 2014

Dispersive liquid-liquid microextraction (DLLME) coupled with gas chromatography-mass spectrometry (GC-MS) was used to quantify and semi-quantify 304 pesticides and related organic pollutants in surface water. Extraction parameters including types and volumes of extraction solvent, disperser solvent, and the amount of salt addition were optimized to obtain highest recoveries and lowest detection limits. A mixture of 35 μL carbon tetrachloride (extraction solvent) and 1 mL acetone (disperser solvent) was added into 5 mL of water sample. After agitation and centrifugation, 20 μL of the sedimented phase was mixed with 1 μL of the internal standard solution, and 1 μL of the final solution was injected into GC-MS for separation and quantification. The enrichment factors ranging from 42- to 299-fold were obtained for all compounds. The limits of detection ranged from 0.001 to 1.125 μg L-1, and the limits of quantification ranged from 0.003 to 3.75 μg L-1. Of all 304 compounds, 90 compounds showed recoveries from 60% to 120% and RSDs lower than 20%. The proposed method is very low cost, rapid and convenient, and could be an effective method for the monitoring of multiple pesticides in surface water. © The Royal Society of Chemistry 2014.


Chen B.,Sun Yat Sen University | Chen B.,Shenzhen Key Laboratory of Detection Technology for Food Safety | Wu F.-Q.,Shenzhen Key Laboratory of Detection Technology for Food Safety | Wu W.-D.,Shenzhen Key Laboratory of Detection Technology for Food Safety | And 4 more authors.
Microchemical Journal | Year: 2016

A low solvent consumption method was developed to determine 27 different classes of pesticides (including organochlorine pesticide, organophosphorus pesticide, pyrethroid pesticide, fungicide, herbicide and acaricide) in wine using dispersive liquid-liquid microextraction (DLLME). Extraction parameters including type and volume of extraction solvent, type and volume of disperser solvent, salinity, pH, centrifugation time, vortex extraction time and wine volumes were optimized. A mixture of 60. μL chloroform (extraction solvent) and 940. μL acetonitrile (disperser solvent) was injected into 5. mL wine diluent. After shaking and centrifugation, the sedimented phase was transferred into a 200. μL glass insert and determined by gas chromatography-mass spectrometry method (GC-MS). The results demonstrated that the recoveries for all the pesticides spiked at three different levels ranged from 66.7 to 126.1%. The intra-day repeatabilities (RSDs) ranged from 2.0 to 27.2%. The limits of detection ranged from 0.025 to 0.88. μg/L, and the limits of quantification ranged from 0.082 to 2.94. μg/L. The proposed method is very low cost, rapid and convenient, and could be an effective method for monitoring of multi-pesticide in wine. © 2015 Elsevier B.V.

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