Shenzhen International Travel Health Care Center

Shenzhen, China

Shenzhen International Travel Health Care Center

Shenzhen, China
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Ma X.,Chinese Academy of Inspection and Quarantine | Xu H.,Chinese Academy of Inspection and Quarantine | Shi L.,Shenzhen International Travel Health Care Center | Yang P.,Centers for Disease Control and Prevention | And 4 more authors.
BMC Infectious Diseases | Year: 2015

Background: A cost-effective, accurate and rapid simultaneous multiplex assay is required for testing and diagnoses of conventional and emerging viruses in clinical virology laboratories. We developed and optimized a dual priming oligonucleotide (DPO) multiplex PCR assay for detecting influenza viruses including seasonal H1N1, 2009 pandemic H1N1, H3N2, influenza B and H5N1. Methods: The optimized multiplex DPO PCR was used to detect 233 clinical human samples. The results were compared to those obtained with RT-qPCR, conventional PCR and immunochromatographic assay. Results: Specificity analysis revealed that the DPO PCR assay amplified each target virus without any cross-amplification. Statistical analysis demonstrated that the multiplex DPO-PCR sensitivity was higher than for the immunochromatographic assay and lower than for qPCR, while no significant difference was observed compared with conventional PCR, when detecting influenza A and B. Additional experiments using the same sample panel indicated no significant differences between the number of positive samples detected by multiplex DPO PCR and RT-qPCR when applying a Cq with a value lower than 30. Conclusions: The five-targeted simultaneous multiplex DPO PCR assay could be easily adopted into routine practice. This approach is cost effective with a short running time, low technical requirements for the detection of influenza virus and early diagnosis in clinical laboratories. © 2015 Ma et al.; licensee BioMed Central.


Dong R.,Shenzhen International Travel Health Care Center | Dong R.,National Center for Control and Prevention | Qiao X.,Centers for Disease Control and Prevention | Jia W.,Centers for Disease Control and Prevention | And 8 more authors.
Biomedical and Environmental Sciences | Year: 2011

Background: Unhygienic blood collection in the early 1990s led to blood-borne infections in Central China. This study aimed to estimate human immunodeficiency virus (HIV) co-infection with hepatitis C and B viruses (HCV and HBV) and their risk factors in a rural area of Shanxi Province with a history of commercial blood donation. Methods: A cross-sectional study was conducted in 2004. All adult residents in the target area were invited to participate in the study. Face-to-face interviews were completed and blood specimens were tested for HIV, HCV, and HBV surface antigen (HBsAg). Results: Prevalence rates of HIV, HCV, and HBsAg were 1.3% (40/3 062), 12.7% (389/3 062), and 3.5% (103/2982), respectively. Of the 40 HIV-positive specimens, 85% were HCV positive and 2.5% were HBsAg positive. The history of commercial blood donation was positively associated with HIV, HCV, and HIV/HCV co-infections, but was negatively associated with HBsAg seropositivity. Migration for employment in the last 5 years was positively related to HIV, HBsAg, and HIV/HCV co-infections. Univariate logistic analysis showed that illegal drug use, number of sex partners, extramarital sex behavior, commercial sex behavior, and condom use rate were not related to anti-HIV, anti-HCV, HBsAg seropositivity or their co-infections. Conclusion: The history of commercial blood donation was the main risk factor for HIV, HCV, and HIV/HCV co-infections in this former commercial blood donation area. HIV and HCV prevention and treatment interventions are important in this area. © 2011 The Editorial Board of Biomedical and Environmental Sciences.


PubMed | Centers for Disease Control and Prevention, Shenzhen International Travel Health Care Center and Chinese Academy of Inspection and Quarantine
Type: | Journal: BMC infectious diseases | Year: 2015

A cost-effective, accurate and rapid simultaneous multiplex assay is required for testing and diagnoses of conventional and emerging viruses in clinical virology laboratories. We developed and optimized a dual priming oligonucleotide (DPO) multiplex PCR assay for detecting influenza viruses including seasonal H1N1, 2009 pandemic H1N1, H3N2, influenza B and H5N1.The optimized multiplex DPO PCR was used to detect 233 clinical human samples. The results were compared to those obtained with RT-qPCR, conventional PCR and immunochromatographic assay.Specificity analysis revealed that the DPO PCR assay amplified each target virus without any cross-amplification. Statistical analysis demonstrated that the multiplex DPO-PCR sensitivity was higher than for the immunochromatographic assay and lower than for qPCR, while no significant difference was observed compared with conventional PCR, when detecting influenza A and B. Additional experiments using the same sample panel indicated no significant differences between the number of positive samples detected by multiplex DPO PCR and RT-qPCR when applying a Cq with a value lower than 30.The five-targeted simultaneous multiplex DPO PCR assay could be easily adopted into routine practice. This approach is cost effective with a short running time, low technical requirements for the detection of influenza virus and early diagnosis in clinical laboratories.


Shi L.,Jinan University | Wu F.,Tsinghua University | Wen Y.,Jinan University | Zhao F.,Shenzhen International Travel Health Care Center | And 2 more authors.
Analytical and Bioanalytical Chemistry | Year: 2015

A novel strip test system combining immunomagnetic separation with lateral flow immunoassay (LFIA) was established for the accurate detection of Listeria monocytogenes. In this system, a pair of matched monoclonal antibodies was used to construct a sandwich immunoassay, in which superparamagnetic particles were coupled with one of the antibodies as a labeled antibody to capture the target bacteria, while the other antibody was immobilized on the detection zone. After a 20-min reaction, the strips were analyzed by a novel instrument which could detect the magnetic signal of the immunocomplex in a magnetic field. Sensitivity evaluation showed that the limit of detection (LOD) of the superparamagnetic LFIA system for L. monocytogenes was 104 CFU/mL, which was at least one log lower than conventional LFIA. No cross-reaction was observed when Salmonella, Escherichia coli O157:H7, or three types of harmless Listeria strains were tested. Further evaluation with actual food samples indicated that the superparamagnetic LFIA system showed 100 % concordance with real-time PCR. Therefore, this novel superparamagnetic LFIA system could be used as a rapid, sensitive, and specific method for the detection of L. monocytogenes. [Figure not available: see fulltext.] © 2014 Springer-Verlag Berlin Heidelberg.


PubMed | Jilin University, Shenzhen International Travel Health Care Center, Suzhou SJ Biomaterials Co. Suzhou and CAS Suzhou Institute of Nano Technology and Nano Bionics
Type: | Journal: Scientific reports | Year: 2015

Linear B-cell epitopes are ideal biomarkers for the serodiagnosis of infectious diseases. However, the long-predicted diagnostic value of epitopes has not been realized. Here, we demonstrated a method, diagnostic epitopes in four steps (DEIFS), that delivers a combination of epitopes for the serodiagnosis of infectious diseases with a high success rate. Using DEIFS for malaria, we identified 6 epitopes from 8 peptides and combined them into 3 chimeric peptide constructs. Along with 4 other peptides, we developed a rapid diagnostic test (RDT), which is able to differentiate Plasmodium falciparum (P. falciparum) from Plasmodium vivax (P. vivax) infections with 95.6% overall sensitivity and 99.1% overall specificity. In addition to applications in diagnosis, DEIFS could also be used in the diagnosis of virus and bacterium infections, discovery of vaccine candidates, evaluation of vaccine potency, and study of disease progression.


Nie Y.,Zhejiang University of Technology | Liang X.-J.,Zhejiang University of Technology | Lu M.-Z.,Zhejiang University of Technology | Yu F.-W.,Zhejiang University of Technology | And 3 more authors.
Journal of Zhejiang University: Science A | Year: 2014

The mass transfer and reaction kinetics of sulfuryl fluoride (SO2F2) absorption with aqueous sodium hydroxide (NaOH) solutions were studied in an experimental double-stirred cell. Results showed that SO2F2 absorption with NaOH was followed by a reaction model employing a fast pseudo-first-order. The second-order rate constant for SO2F2 absorption with aqueous NaOH solutions was determined to be 1.44 m3/(mols) at 298 K. Three models were used in this chemical absorption process, and in each case, the same expression of enhancement factor was obtained. A comparison was made between the experimental enhancement factor and the value calculated from the model, and the maximum relative deviation was less than 4.2%. The proposed model expression gave a reasonable fit with the experimental values, indicating that mass transfer correlations are valid for scaling up design. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2014.


Nie Y.,Zhejiang University of Technology | Liang X.,Zhejiang University of Technology | Ji J.,Zhejiang University of Technology | Lu M.,Zhejiang University of Technology | And 4 more authors.
Environmental Engineering Science | Year: 2015

Chemical absorption was employed for the harmless treatment of sulfuryl fluoride (SO2F2) in the simulated residual fumigant. SO2F2 was absorbed by sodium hydroxide (NaOH) solution in a packed column. Effects of spray density, gas flow rate, concentration of NaOH solution, and inlet SO2F2 volume concentration on SO2F2 removal efficiency were investigated at room temperature. It was found that SO2F2 could be removed completely when inlet SO2F2 volume concentration, spray density, gas flow rate, and NaOH concentration were 0.50%, 12 m3/(m2 · h), 0.12 m3/h, and 0.68 mol/L, respectively. According to the two-film theory, a mathematical model based on Onda correlations was developed to describe the removal efficiency of SO2F2. Comparison between the experimental data and the calculated data proved that the predictability of the proposed model was within the maximum deviation of 8.0%. Aqueous NaOH solution after absorption was analyzed. It was found that the SO2F2 was fixed and converted to NaF, NaSO3F, and Na2SO4. Copyright © 2015, Mary Ann Liebert, Inc.


Nie Y.,Zhejiang University of Technology | Zheng Q.,Zhejiang University of Technology | Liang X.,Zhejiang University of Technology | Gu D.,Shenzhen International Travel Health Care Center | And 3 more authors.
Environmental Science and Technology | Year: 2013

The technology of packed bed dielectric barrier discharge (DBD) plasma followed by a chemical absorption has been developed and was found to be an efficient way for decomposition treatment of sulfuryl fluoride (SO 2F2) in simulated residual fumigant. The effects of energy density, initial SO2F2 concentration, and residence time on the removal efficiency of SO2F2 for the DBD plasma treatment alone were investigated. It was found that the SO2F 2 could be removed completely when initial volume concentration, energy density, and residence time were 0.5%, 33.9 kJ/L, and 5.1 s, respectively. The removal mechanism of SO2F2 in the packed bed DBD reactor was discussed. Based on the detailed analysis of SO 2F2 molecular stability and its exhaust products in the DBD plasma reactor, it was concluded that the energetic electrons generated in the packed bed DBD reactor played a key role on the removal of SO 2F2, and the major decomposition products of SO 2F2 detected were SO2, SiF4, and S (Sulfur). Among these products, SiF4 was formed by the F atom reacted with the filler-quartz glass beads (SiO2) in the packed bed DBD reactor. Aqueous NaOH solution was used as the chemical absorbent for the gaseous products of SO2F2 after plasma pretreatment. It was found that the gaseous products in the plasma exhaust could be absorbed and fixed by the subsequent aqueous NaOH solution. © 2013 American Chemical Society.


Zheng Q.,Zhejiang University of Technology | Nie Y.,Zhejiang University of Technology | Gu D.,Shenzhen International Travel Health Care Center | Huang T.,Shenzhen International Travel Health Care Center | And 2 more authors.
Gaodianya Jishu/High Voltage Engineering | Year: 2012

Alumina and quartz glass balls were chosen as filling dielectric, and the packed bed dielectric barrier discharge (DBD) plasma was adopted to realize the harmless treatment of sulfuryl fluoride (SO 2F 2). The effects of discharge voltage and energy density on the removal efficiency of SO 2F 2 filled with different dielectrics were investigated, respectively. Meanwhile, the removal products and mechanism of SO 2F 2 filled with different dielectrics in the DBD reactor were analyzed. Results showed that alumina dielectric was more effective for SF 2O 2 harmless treatment, and SF 2O 2 could be completely removed when the energy density was above 208 J/L. Compared with packing quartz glass balls, the energy consumption of packing alumina ball decreased by 50%. SO 2F 2 was degraded into SO 2, S and SiF4 when quartz glass balls was filled in the DBD reactor. In terms of alumina dielectric, the harmful gaseous products after plasma treatment could be completely reacted and absorbed by alumina to realize the harmless treatment of SF 2O 2.


PubMed | Shenzhen International Travel Health Care Center
Type: Journal Article | Journal: Biomedical and environmental sciences : BES | Year: 2011

Unhygienic blood collection in the early 1990s led to blood-borne infections in Central China. This study aimed to estimate human immunodeficiency virus (HIV) co-infection with hepatitis C and B viruses (HCV and HBV) and their risk factors in a rural area of Shanxi Province with a history of commercial blood donation.A cross-sectional study was conducted in 2004. All adult residents in the target area were invited to participate in the study. Face-to-face interviews were completed and blood specimens were tested for HIV, HCV, and HBV surface antigen (HBsAg).Prevalence rates of HIV, HCV, and HBsAg were 1.3% (40/3 062), 12.7% (389/3 062), and 3.5% (103/2982), respectively. Of the 40 HIV-positive specimens, 85% were HCV positive and 2.5% were HBsAg positive. The history of commercial blood donation was positively associated with HIV, HCV, and HIV/HCV co-infections, but was negatively associated with HBsAg seropositivity. Migration for employment in the last 5 years was positively related to HIV, HBsAg, and HIV/HCV co-infections. Univariate logistic analysis showed that illegal drug use, number of sex partners, extramarital sex behavior, commercial sex behavior, and condom use rate were not related to anti-HIV, anti-HCV, HBsAg seropositivity or their co-infections.The history of commercial blood donation was the main risk factor for HIV, HCV, and HIV/HCV co-infections in this former commercial blood donation area. HIV and HCV prevention and treatment interventions are important in this area.

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