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Tao H.,Peoples Care | Xu Y.P.,Shenzhen Blood Center | Chen L.X.,Peoples Care
Tissue Antigens | Year: 2014

Genomic full-length sequences of HLA-A*02:01:01:01 and HLA-A*02:03:01 were identified by cloning and sequencing from two Chinese donors. © 2014 John Wiley and Sons A/S. Source


Xu Y.P.,Shenzhen Blood Center
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2010

In the present study, a high-resolute method for cloning and sequencing genomic full-length HLA-A and -B using 20 Chinese Han individuals was established. We detected 10 HLA-A allele sequences 4.2 kb in length and 6 HLA-B allele sequences 3.7 kb in length, and the sequences included all exons, all introns, 5'promoter, and 3'UTR of the two genes. All sixteen sequences have been submitted to GenBank and IMGT/HLA database. A*1153 is a novel allele, and the introns of B*151101 are firstly reported here. The 5'promoter and 3'UTR sequences of 5 HLA-A alleles and 2 HLA-B alleles are also firstly disclosed, and all other alleles have extended the genomic full length sequences released in IMGT/HLA database. The polymorphic structures of upper 5'promoter and downstream 3'UTR, which were uncovered in IMGT/HLA database, are firstly depicted in Chinese Han individuals. Twenty-six single nucleotide polymorphisms (SNPs) and one 3 bp-insertion/deletion (Indel) were located in the upper 5'promoter and 14 SNPs were located in the 3'UTR of HLA-A. In addition, five SNPs and one 1 bp-indel were located in the upper 5'promoter and 5 SNPs were located in the 3'UTR of HLA-B. Through analyzing the phylogenetic trees of 5'promoter, exons and 3'UTR of the two genes, we found that the evolution history of regulatory regions and exons is different between the two genes. The regulatory regions are tightly linked with exons in most of HLA-A alleles excluding A*24020101. On the contrary, recombinant events may occur frequently between regulatory regions and exons in most HLA-B alleles. Source


Cao Q.,Southern Medical University | Cao Q.,Shenzhen Blood Center
PloS one | Year: 2014

BACKGROUND: End-Stage Renal Disease (ESRD) is a worldwide public health problem. Currently, many genome-wide association studies have suggested a potential association between human leukocyte antigen (HLA) and ESRD by uncovering a causal relationship between HLA and glomerulonephritis. However, previous studies, which investigated the HLA polymorphism and its association with ESRD, were performed with the modest data sets and thus might be limited. On the other hand, few researches were conducted to tackle the Chinese population with ESRD. Therefore, this study aims to detect the susceptibilities of HLA polymorphism to ESRD within the Cantonese community, a representative southern population of China.METHODS: From the same region, 4541 ESRD patients who were waiting for kidney transplantation and 3744 healthy volunteer bone marrow donors (controls) were randomly chosen for this study. Polymerase chain reaction-sequence specific primer method was used to analyze the HLA polymorphisms (including HLA-A, HLA-B and HLA-DRB1 loci) in both ESRD patients and controls. The frequencies of alleles at these loci and haplotypes were compared between ESRD patients and controls.RESULTS: A total of 88 distinct HLA alleles and 1361 HLA A-B-DRB1 haplotypes were detected. The frequencies of five alleles, HLA-A*24, HLA-B*55, HLA-B*54, HLA-B*40(60), HLA-DRB1*04, and one haplotype (HLA-A*11-B*27-DRB1*04) in ESRD patients are significantly higher than those in the controls, respectively.CONCLUSIONS: Five HLA alleles and one haplotype at the HLA-A, HLA-B and HLA-DRB1 loci appear to be associated with ESRD within the Cantonese population. Source


Xu Y.P.,Shenzhen Blood Center | Gao S.Q.,Shenzhen Blood Center
Tissue Antigens | Year: 2014

Genomic full length sequences of HLA-A*11:01:01 and A*11:02:01 were identified by cloning and sequencing from two different Chinese donors. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Source


Zhang H.,Shenzhen Blood Center | Xu Y.P.,Shenzhen Blood Center
Tissue Antigens | Year: 2014

Genomic full-length sequences of HLA-A*02:05:01 and A*02:06:01, were identified by cloning and sequencing from two Chinese donors. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Source

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