PubMed | Wuxi Maternal and Child Health Hospital, Shenzhen Baoan Maternal and Child Health Hospital and Nanchang University
Type: Journal Article | Journal: Archives of gynecology and obstetrics | Year: 2016
Our previous study used freeze-drying and biotin-avidin binding methods and obtained nontargeted nanobubbles (N-NBs) and ovarian cancer-targeting nanobubbles (LHRH-NBs, luteinizing hormone-releasing hormone nanobubbles). Our study also identified the physical and chemical properties of these two contrast agents, and validated the targeting ability and underlying mechanisms of LHRH-NBs in vitro. The present study investigated the imaging of N-NBs and LHRH-NBs in nude mice and their binding with tissues.The nude mice models of xenografts were divided into three groups, N-NB, LHRH-NB, and SonoVue. These contrast agents were injected via the caudal vein to observe the imaging of ovarian cancer. Fluorescence microscope was used to observe the penetration of N-NBs and LHRH-NBs through the vascular endothelial gaps. Immunofluorescence was used to observe the penetration of N-NBs and LHRH-NBs through vascular endothelial gaps and binding to the tumor cells.The imaging intensity and duration were not significantly different between N-NBs and LHRH-NBs. The imaging intensity in the N-NB and LHRH-NB groups was not significantly different compared with the SonoVue group; however, the imaging duration in the N-NB and LHRH-NB groups was significantly longer than in the SonoVue group (P<0.001). Both N-NBs and LHRH-NBs penetrated through the vascular endothelial gaps. After penetrating through the vascular endothelial gapes, LHRH-NBs could target and bind to the tumor cells.N-NBs and LHRH-NBs are of good imaging effectiveness and relatively long imaging duration. LHRH-NB is a potent contrast agent for imaging ovarian cancer, while achieving targeted delivery of drugs to the site of ovarian cancer.
Hong F.-C.,U.S. Center for Disease Control and Prevention |
Yang Y.-Z.,U.S. Center for Disease Control and Prevention |
Liu X.-L.,U.S. Center for Disease Control and Prevention |
Feng T.-J.,U.S. Center for Disease Control and Prevention |
And 5 more authors.
Sexually Transmitted Diseases | Year: 2014
Background: Untreated maternal syphilis can result in the fetuses being infected. Severe adverse pregnancy outcomes include stillbirth, perinatal death, low birth weight, and congenital syphilis (CS). The World Health Organization has already classified global elimination of CS as a priority. However, this preventable disease is still threatening people's health in the world. METHODS: A Programme of Prevention of Mother-to-Child Transmission of Syphilis in Shenzhen was launched in 2002. All pregnant women in Shenzhen were screened for syphilis by serological methods at their first prenatal care visit. The infected individuals were treated with 3 weekly injections of 2.4 million units of benzathine penicillin. The babies were followed up until 18 months old to diagnose CS. RESULTS: Up to 2011, the Programme of Prevention of Mother-to-Child Transmission of Syphilis in Shenzhen screened 2,077,362 pregnant women and intervened in 7668 mothers infected with syphilis. The screened rate among pregnant women increased from 89.8% in 2002 to 97.4% in 2011. The proportion of those having adverse pregnant outcomes (including spontaneous abortion, premature delivery, and stillbirth) decreased from 27.3% in 2003 to 8.2% in 2011. The incidence of CS decreased from 115/100,000 in 2002 to 10/100,000 (live births) in 2011. CONCLUSIONS: In 2002, in the face of rising CS numbers, Shenzhen adapted a syphilis control program that involved cost-free testing for pregnant women, commitment and collaboration at multiple levels of the health system, and strong supervision and government guidance. Local program and surveillance data suggest that the program has been very successful in reducing CS incidence. © 2014 American Sexually Transmitted Diseases Association All rights reserved.
PubMed | China Medical University at Taichung, University of Florida and Shenzhen Baoan Maternal and Child Health Hospital
Type: | Journal: Paediatric respiratory reviews | Year: 2016
Bronchopulmonary dysplasia (BPD) is a frequent complication occurring in extremely preterm infants. Despite recent advances in newborn medicine, the incidence of BPD does not appear to have changed markedly, and specific treatments and prevention strategies are still lacking. Nutrition plays an important role in normal lung development and maturation. Malnutrition may delay somatic growth and new alveoli development, thus aggravating pulmonary injury involved in the pathogenesis of BPD. However, few nutrients have been investigated for their potential to mitigate the pathogenesis of BPD. In this article, we reviewed the recent progress in research on potential nutrients useful for the prevention or treatment of BPD, including glutamine, cysteine and N-acetylcysteine, L-arginine and L-citrulline, long chain polyunsaturated fatty acids (LCPUFAs), inositol, selenium, and some antioxidant vitamins including vitamin A. Current evidence shows that vitamin A and LCPUFA can prevent BPD, and that L-citrulline might provide a new method to treat chronic pulmonary hypertension associated with BPD in premature infants. The effects of other nutrients on BPD prevention need to be further studied.
Hu G.,Southern Medical University |
Hu G.,Shenzhen baoAn Maternal and Child Health Hospital |
Chan Q.,Philips |
Quan X.,Southern Medical University |
And 4 more authors.
Journal of Magnetic Resonance Imaging | Year: 2015
Purpose To explore the characteristics of intravoxel incoherent motion (IVIM) in various stages of liver fibrosis, and their relationships with fibrotic stages in rats. Materials and Methods Fifty rats were given various doses of carbon tetrachloride (CCl4) to induce various fibrotic stages in rats; 15 untreated rats served as controls. Diffusion-weighted magnetic resonance imaging (MRI) was performed and eight b-values (0-800 s/mm2) were applied to obtain IVIM parameters (D, pure molecular diffusion; f, perfusion fraction; D∗, pseudodiffusion). The stages of liver fibrosis (stages F0-F4) were evaluated histologically using METAVIR scores. Fifty-seven rats (15 controls and 42 with fibrosis) were analyzed by nonparametric methods and receiver operating characteristic curves to determine diagnostic accuracy. Results Significant differences (P-<-0.001) were found between stages (stages F0-F4) by D, f, D∗, and apparent diffusion coefficient (ADC). There were inverse correlations between fibrosis stages and D, f, D∗, ADC (r-=--0.657, r-=--0.631, r-=--0.711 r-=--0.719, respectively). Multivariate analysis showed that the combination models (D, f, D∗) were better than the individual parameter (ADC) for the evaluation fibrosis stages (area under the curve [AUC]: 0.821-1.000 vs. AUC: 0.753-0.918) Conclusion IVIM-derived parameters showed significant correlations with stages of liver fibrosis in a rat model. © 2014 Wiley Periodicals, Inc.
Du J.,Guangzhou University |
Li L.,Guangdong Medical College |
Dou Y.,Guangzhou University |
Li P.,Guangzhou University |
And 2 more authors.
PLoS ONE | Year: 2014
Introduction: Neutrophil CD64 has been proposed as an early marker of sepsis. This study aims to evaluate the diagnostic utility of neutrophil CD64 for identification of early-onset sepsis in preterm neonates. Methods: The prospective study was conducted in a neonatal intensive care unit between November 2010 and June 2011. Preterm neonates in whom infection was suspected when they were <12 hours of age were enrolled. Complete blood count with differential, blood culture, neutrophil CD11b and CD64 measurement were performed. Receiver operating characteristic curve analysis was performed to evaluate the performance of neutrophil CD64 as biomarker of sepsis. Results: A total of 158 preterm neonates was enrolled, 88 of whom were suspected infection. The suspected sepsis group was of lesser gestational age (P<0.001) and lower birth weight (P<0.001), compared with controls. The hematologic profiles of the suspected sepsis group were characterized by higher white blood cell count, neutrophil counts and C-reactive protein. The suspected sepsis neonates had significantly higher neutrophil CD64 expression compared with controls. Neutrophil CD64 had an area value under the curve of 0.869 with an optimal cutoff values of 1010 phycoerythrin molecules bound/cell and it had a high sensitivity (81.82%) and negative predictive value (77.4%). The level of neutrophil CD64 was independent of antibiotic therapy within 24 hours after the onset of sepsis in preterm neonates. Conclusions: Neutrophil CD64 is a highly sensitive marker for suspected early-onset sepsis in preterm neonates. Our study suggests that neutrophil CD64 may be incorporated as a valuable marker to diagnose infection. © 2014 Du et al.
PubMed | Philips, Southern Medical University, Jinan University and Shenzhen Baoan Maternal and Child Health Hospital
Type: Journal Article | Journal: Journal of magnetic resonance imaging : JMRI | Year: 2016
To explore the value of T1 relaxation times in the rotating frame (T1 or T1 rho) for evaluating liver fibrosis stage, compared to apparent diffusion coefficients (ADCs).Liver fibrosis in model rats (n=50) was produced by carbon tetrachloride (CCl4 ) injection. Five rats died during the experiment. Surviving model rats (n=45) and controls (n=15) were subjected to 3.0T MRI and the ADCs (b-values: 0, 800s/mm(2) ) and T1 values were determined. Liver fibrosis stage (F0-F4) was defined based on METAVIR scoring. Nonparametric statistical methods and receiver operating characteristic (ROC) curve analyses were employed to determine diagnostic accuracy.Mean ADC and T1 associated negatively (r=-0.732 P<0.001) and positively (r=0.863 P<0.001), respectively, with severity of fibrosis stage. Analysis of ROC curves for fibrosis staging showed that the area under the curve (AUC) for T1 (stage F0 vs. F1-F4=0.976, stage F0-F1 vs. F2-F4=0.920, stage F0-F2 vs. F3-F4=0.938, and stage F0-F3 vs. F4=0.931) was larger than that for ADCs (0.917, 0.924, 0.842, and 0.781, respectively).ADC and T1 values correlate with liver fibrosis stage. The performance of the T1 parameter was superior to that of the ADC parameter in the differentiation of liver fibrosis stages in a CCl4 rat model.
Lou Y.,Huazhong University of Science and Technology |
Wu C.,Huazhong University of Science and Technology |
Wu M.,Huazhong University of Science and Technology |
Xie C.,Shenzhen Baoan Maternal and Child Health Hospital |
Ren L.,Shenzhen Baoan Maternal and Child Health Hospital
Diabetes Research and Clinical Practice | Year: 2014
Aims: To investigate plasma levels and the expression of neutrophil gelatinase-associated lipocalin (NGAL) in subcutaneous adipose tissue (SAT) in patients with gestational diabetes mellitus (GDM). Methods: The study recruited 260 Chinese women divided into three groups: 96 were healthy pregnant women with pre-pregnancy body mass index (pre-pregnancy BMI) below 25kg/m2 (GROUP 1), 84 were women with GDM with pre-pregnancy BMI below 25kg/m2 (GROUP 2) and 80 were women with GDM with pre-pregnancy BMI over 25kg/m2 (GROUP 3). Laboratory and anthropometric measurements were recorded and NGAL plasma levels were determined by ELISA for subjects in all groups. Real-time RT-PCR and Western blotting were used to assess the relative mRNA and protein expression of NGAL and tumor necrosis factor-α (TNF-α) in SAT (30 cases in each group). Results: Our results demonstrated statistically significant elevation in plasma NGAL concentrations in GROUP 2 and GROUP 3 compared with GROUP 1 (p< 0.001 for both group comparisons). Moreover, SAT NGAL mRNA (p< 0.001 and p< 0.001, respectively) and protein (p< 0.001 and p< 0.001, respectively) expression levels were higher in GROUP 3 than in both GROUP 1 and GROUP 2. Correlations were noted between the plasma NGAL concentration and various parameters of insulin resistance. Conclusions: Plasma NGAL may play a role in the development of insulin resistance in GDM, and the high levels of NGAL expression in SAT in overweight women with GDM suggests that NGAL in SAT is associated with obesity in women with GDM. © 2014 Elsevier Ireland Ltd.
Xie C.,Shenzhen Baoan Maternal and Child Health Hospital |
Yao M.Z.,Shenzhen Baoan Maternal and Child Health Hospital |
Liu J.B.,Shenzhen Baoan Maternal and Child Health Hospital |
Xiong L.K.,Shenzhen Baoan Maternal and Child Health Hospital
Cytokine | Year: 2011
Inflammation may play a major role in the pathogenesis of preeclampsia (PE). In this meta-analysis, we determined whether maternal polymorphisms and serum concentrations of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) were associated with PE. All studies investigating the associations between PE and maternal polymorphisms of TNF-α-308G/A, IL-6-174G/C, and IL-10-1082A/G or serum concentrations of TNF-α, IL-6, and IL-10 were reviewed. We found that neither maternal TNF-α-308G/A (p= 0.86, odds ratio [OR] = 0.98, 95% confidence interval [CI], 0.76-1.25), IL-6 174G/C (p= 0.14, OR = 1.23, 95% CI, 0.93-1.61), nor IL-10-1082A/G (p= 0.72, OR = 1.07, 95% CI, 0.75-1.52) were associated with PE. On the other hand, maternal TNF-α (p< 0.00001, weighted mean difference [WMD] = 19.63. pg/ml, 95% CI, 18.54-20.72. pg/ml), IL-6 (p< 0.00001, WMD = 6.58. pg/ml, 95% CI, 5.49-7.67. pg/ml), and IL-10 (p= 0.0005, WMD = 19.30. pg/ml, 95% CI, 8.42-30.17. pg/ml) concentrations were significantly higher in PE patients versus controls. Our findings strengthen the clinical evidence that PE is accompanied by exaggerated inflammatory responses, but do not support TNF-α-308G/A, IL-6-174G/C, and IL-10-1082A/G as candidate susceptibility loci in PE. © 2011 Elsevier Ltd.
Xia Y.,Shenzhen Baoan Maternal and Child Health Hospital |
Xiong L.K.,Shenzhen Baoan Maternal and Child Health Hospital
Chinese Medical Journal | Year: 2014
Objective To review the common genotyping techniques of Chlamydia trachomatis in terms of their principles, characteristics, applications and limitations.Data sources Data used in this review were mainly from English literatures of PubMed database. The search terms were “Chlamydia trachomatis” and “genotyping”. Meanwhile, data from World Health Organization were also cited.Study selection Original articles and reviews relevant to present review’s theme were selected.Results Different genotyping techniques were applied on different occasions according to their characteristics, especially in epidemiological studies worldwide, which pushed the study of Chlamydia trachomatis forward greatly. In addition, summaries of some epidemiological studies by genotyping were also included in this work for reference and comparison.Conclusions A clear understanding of common genotyping techniques could be helpful to genotype C. trachomatis more appropriately and effectively. Furthermore, more studies on the association of genotypes of Chlamydia trachomatis with clinical manifestations should be performed. © 2014, Chinese Medical Journal. All rights reserved.
PubMed | Shenzhen Baoan Maternal and Child Health Hospital
Type: Journal Article | Journal: HLA | Year: 2016
HLA-A*02:431 differs from the closest allele HLA-A*02:03:01 by a nonsynonymous mutation at nucleotide 317 in exon 2.