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Hu G.,Southern Medical University | Chan Q.,Philips | Quan X.,Southern Medical University | Zhang X.,Southern Medical University | And 3 more authors.
Journal of Magnetic Resonance Imaging | Year: 2015

Purpose To explore the characteristics of intravoxel incoherent motion (IVIM) in various stages of liver fibrosis, and their relationships with fibrotic stages in rats. Materials and Methods Fifty rats were given various doses of carbon tetrachloride (CCl4) to induce various fibrotic stages in rats; 15 untreated rats served as controls. Diffusion-weighted magnetic resonance imaging (MRI) was performed and eight b-values (0-800 s/mm2) were applied to obtain IVIM parameters (D, pure molecular diffusion; f, perfusion fraction; D∗, pseudodiffusion). The stages of liver fibrosis (stages F0-F4) were evaluated histologically using METAVIR scores. Fifty-seven rats (15 controls and 42 with fibrosis) were analyzed by nonparametric methods and receiver operating characteristic curves to determine diagnostic accuracy. Results Significant differences (P-<-0.001) were found between stages (stages F0-F4) by D, f, D∗, and apparent diffusion coefficient (ADC). There were inverse correlations between fibrosis stages and D, f, D∗, ADC (r-=--0.657, r-=--0.631, r-=--0.711 r-=--0.719, respectively). Multivariate analysis showed that the combination models (D, f, D∗) were better than the individual parameter (ADC) for the evaluation fibrosis stages (area under the curve [AUC]: 0.821-1.000 vs. AUC: 0.753-0.918) Conclusion IVIM-derived parameters showed significant correlations with stages of liver fibrosis in a rat model. © 2014 Wiley Periodicals, Inc. Source


Hong F.-C.,U.S. Center for Disease Control and Prevention | Yang Y.-Z.,U.S. Center for Disease Control and Prevention | Liu X.-L.,U.S. Center for Disease Control and Prevention | Feng T.-J.,U.S. Center for Disease Control and Prevention | And 5 more authors.
Sexually Transmitted Diseases | Year: 2014

Background: Untreated maternal syphilis can result in the fetuses being infected. Severe adverse pregnancy outcomes include stillbirth, perinatal death, low birth weight, and congenital syphilis (CS). The World Health Organization has already classified global elimination of CS as a priority. However, this preventable disease is still threatening people's health in the world. METHODS: A Programme of Prevention of Mother-to-Child Transmission of Syphilis in Shenzhen was launched in 2002. All pregnant women in Shenzhen were screened for syphilis by serological methods at their first prenatal care visit. The infected individuals were treated with 3 weekly injections of 2.4 million units of benzathine penicillin. The babies were followed up until 18 months old to diagnose CS. RESULTS: Up to 2011, the Programme of Prevention of Mother-to-Child Transmission of Syphilis in Shenzhen screened 2,077,362 pregnant women and intervened in 7668 mothers infected with syphilis. The screened rate among pregnant women increased from 89.8% in 2002 to 97.4% in 2011. The proportion of those having adverse pregnant outcomes (including spontaneous abortion, premature delivery, and stillbirth) decreased from 27.3% in 2003 to 8.2% in 2011. The incidence of CS decreased from 115/100,000 in 2002 to 10/100,000 (live births) in 2011. CONCLUSIONS: In 2002, in the face of rising CS numbers, Shenzhen adapted a syphilis control program that involved cost-free testing for pregnant women, commitment and collaboration at multiple levels of the health system, and strong supervision and government guidance. Local program and surveillance data suggest that the program has been very successful in reducing CS incidence. © 2014 American Sexually Transmitted Diseases Association All rights reserved. Source


Du J.,Guangzhou University | Li L.,Guangdong Medical College | Dou Y.,Guangzhou University | Li P.,Guangzhou University | And 2 more authors.
PLoS ONE | Year: 2014

Introduction: Neutrophil CD64 has been proposed as an early marker of sepsis. This study aims to evaluate the diagnostic utility of neutrophil CD64 for identification of early-onset sepsis in preterm neonates. Methods: The prospective study was conducted in a neonatal intensive care unit between November 2010 and June 2011. Preterm neonates in whom infection was suspected when they were <12 hours of age were enrolled. Complete blood count with differential, blood culture, neutrophil CD11b and CD64 measurement were performed. Receiver operating characteristic curve analysis was performed to evaluate the performance of neutrophil CD64 as biomarker of sepsis. Results: A total of 158 preterm neonates was enrolled, 88 of whom were suspected infection. The suspected sepsis group was of lesser gestational age (P<0.001) and lower birth weight (P<0.001), compared with controls. The hematologic profiles of the suspected sepsis group were characterized by higher white blood cell count, neutrophil counts and C-reactive protein. The suspected sepsis neonates had significantly higher neutrophil CD64 expression compared with controls. Neutrophil CD64 had an area value under the curve of 0.869 with an optimal cutoff values of 1010 phycoerythrin molecules bound/cell and it had a high sensitivity (81.82%) and negative predictive value (77.4%). The level of neutrophil CD64 was independent of antibiotic therapy within 24 hours after the onset of sepsis in preterm neonates. Conclusions: Neutrophil CD64 is a highly sensitive marker for suspected early-onset sepsis in preterm neonates. Our study suggests that neutrophil CD64 may be incorporated as a valuable marker to diagnose infection. © 2014 Du et al. Source


Hu G.,Shenzhen Baoan Maternal and Child Health Hospital | Zhong X.,Jinan University | Zhang X.,Southern Medical University | Liang W.,Southern Medical University | And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016

Background: non-invasive method, apparent diffusion coefficient (ADC) and the liver fibrosis index (LFI), assessment of liver fibrosis has been one of major objectives in the society of hepatologists. Aim: To explore the variable characteristics of ADC and the LFI in different stages of liver fibrosis in rats and to compare their performance in staging liver fibrosis. Methods: Liver fibrosis model rats (N = 50) were produced by carbon tetrachloride (CCl4). Surviving model rats (N = 45) and controls (N =15) were subjected to MRI and RTE, and the ADC and LFI values were analyzed. All animals were sacrificed for pathological examination. The liver fibrosis stage (F0-F4) was defined based on the METAVIR score. Nonparametric statistical methods and receiver operating characteristic (ROC) curve analyses were employed to determine diagnostic accuracy. Results: Correlation analysis showed that the liver fibrosis stage was negatively correlated with ADC (r =-0.732, P < 0.001) and positively correlated with LFI (r = 0.706, P < 0.001). ROC curves showed that the areas under the curve (AUCs) for ADC and LFI in the prediction of the liver fibrosis stage were 0.781-0.924 and 0.824-0.939, respectively. Conclusions: Both the ADC values and LFI values were strongly correlated with the liver fibrosis stages in our rat model. Moreover, the ADC was sensitive in predicting early-stage liver fibrosis, while the LFI was more accurate in predicting predict intermediate-and late-stage liver fibrosis. © 2016, E-Century Publishing Corporation. All rights reserved. Source


Hu G.,Shenzhen Baoan Maternal and Child Health Hospital | Hu G.,Southern Medical University | Zhang X.,Southern Medical University | Liang W.,Southern Medical University | And 6 more authors.
Journal of Magnetic Resonance Imaging | Year: 2016

Purpose To explore the value of T1 relaxation times in the rotating frame (T1ρ or T1rho) for evaluating liver fibrosis stage, compared to apparent diffusion coefficients (ADCs). Materials and Methods Liver fibrosis in model rats (n = 50) was produced by carbon tetrachloride (CCl4) injection. Five rats died during the experiment. Surviving model rats (n = 45) and controls (n = 15) were subjected to 3.0T MRI and the ADCs (b-values: 0, 800 s/mm2) and T1ρ values were determined. Liver fibrosis stage (F0-F4) was defined based on METAVIR scoring. Nonparametric statistical methods and receiver operating characteristic (ROC) curve analyses were employed to determine diagnostic accuracy. Results Mean ADC and T1ρ associated negatively (r = -0.732 P < 0.001) and positively (r = 0.863 P < 0.001), respectively, with severity of fibrosis stage. Analysis of ROC curves for fibrosis staging showed that the area under the curve (AUC) for T1ρ (stage F0 vs. F1-F4 = 0.976, stage F0-F1 vs. F2-F4 = 0.920, stage F0-F2 vs. F3-F4 = 0.938, and stage F0-F3 vs. F4 = 0.931) was larger than that for ADCs (0.917, 0.924, 0.842, and 0.781, respectively). Conclusion ADC and T1ρ values correlate with liver fibrosis stage. The performance of the T1ρ parameter was superior to that of the ADC parameter in the differentiation of liver fibrosis stages in a CCl4 rat model. © 2015 Wiley Periodicals, Inc. Source

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