Hui L.,Shenyang Stomatological Hospital |
Hong T.,Shenyang Stomatological Hospital
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: In recent years, porcelain materials applied in clinical practice are mainly complete crown, different kinds of porcelain substrate materials exhibiting adverse effect on the periodontium of abutment teeth has been attracting more and more attention, some periodontal inflammation problems such as gingival swelling and bleeding are becoming a focus. OBJECTIVE: To evaluate the effect of three kinds of porcelain fused to metal crowns, including Ni-Cr alloy, precious metal and galvanized forming crowns/bridges, on periodontal health. METHODS: According to the aspiration of the patient, 254 teeth from 78 patients were respectively restored by Ni-Cr alloy, precious metal and galvanized forming crowns/bridges. Follow-up visits were performed at one year postoperation, to compare the gingival color, sulcus bleeding index, plaque index and the change of periodontal probing depth. RESULTS AND CONCLUSION: After 1 year, 21 teeth restored by Ni-Cr alloy porcelain fused to metal crowns showed the change of gingival color, but the effect of precious metal porcelain fused to metal crowns and galvanized forming crowns/bridges was little or no on gingival color. Cases restored by Ni-Cr alloy porcelain fused to metal crowns/bridges and precious metal crowns/bridges showed significant difference in sulcus bleeding index between pre- and post-restoration, while galvanized forming crowns showed no significant difference. There are no significant difference in three groups of the plaque index and periodontal probing depth between pre- and post-restoration. Results demonstrated that galvanized forming crowns/bridges have minimal effect on the health of periodontal tissue, then precious metal crowns/bridges, and Ni-Cr alloy porcelain fused to metal crowns/bridges maximal.
Zhao X.-y.,Shenyang Stomatological Hospital |
Zhan D.-s.,Liaoning Medical University |
Sun Y.,Shenyang Stomatological Hospital |
Fan Z.,Shenyang Stomatological Hospital
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011
BACKGROUND: Co-Cr alloy has better biocompatibility, anti-corrosion and stability than Ni-Cr and Ti alloy. It is more and more used extensively. OBJECTIVE: Compared to the Germany BEGO cobalt-chromium alloy, to make initial evaluation to the combination of ceramic to metal of the new-type Co-Cr alloys. METHODS: VITA MK95 porcelain powder of 8 mm×3 mm×1 mm was fused to the two types of alloys of 25 mm×3 mm×0.5 mm and the combination of ceramic to metal of the two types of alloys was tested using three-point flexure bond test and scanning electron microscope. RESULTS AND CONCLUSION: For two kinds of alloys after strength loading, ceramic layer cracked and detached completely at the same time, gray-black oxide film formed on the alloy surface, and there was no significant residual porcelain (P > 0.05). The bonding of ceramic to metal of the new type Co-Cr alloys is greater than the basic value of the required ISO (25 MPa), which do not different from that of the Germany BEGO and meet the clinical requirements.
Chen W.,Shenyang Stomatological Hospital |
Huang G.-L.,Zunyi Medical University
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Fewer ethical issues exist in adult stem cells, and some operating technologies are relatively mature. Therefore, to construct tissue-engineered salivary glands using adult stem cells is very attractive and seductive with an extremely important application prospect. OBJECTIVE: To establish a rat model of salivary gland injury by ligation of the main excretory duct of the submandibular gland and to explore the existing feasibility and location of adult stem cells in the injured models. METHODS: The main excretory duct of the right submandibular glands was ligated in Sprague-Dawley rats. After 1 week, rats were killed to remove the bilateral glands that were then subject to hematoxylin-eosin staining, PAS glycogen staining and immunohistochemical staining for determination of CK-19, Bcl-2, Ki-67. After that, we compared the normal submandibular gland with the damaged model after ligation of main excretory duct. RESULTS AND CONCLUSION: The rats showed differences in the volume and mass of the affected and normal submandibular glands. The normal submandibular gland was oval, ruddy, smooth, soft with an intact envelop. After ligation, the injured submandibular gland appeared to have atrophy with dark red in color, irregular morphology, envelop congestion, and rough texture; the surrounding vessels showed compensatory expansion. PAS-positive gland cells disappeared, CK-19-postive small duct epithelial cells proliferated, and laminin-positive cells that were rarely found in the normal gland existed around the duct. In addition, Bcl-2/Ki-67 positive cells were both increased. These findings indicate that stem/progenitor cells may be located in the periductal area of the submandibular gland; and the model of submandibular gland injury established by ligation of the main excretory duct is effective to activate stem/progenitor cells in the submandibular gland. © 2014, Chinese Journal of Tissue Engineering Research. All Rights Reserved.