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Zhou Y.,Panjin Blood Bank | Li J.-P.,Transfusion Medicine Institute | Li J.-P.,Key Laboratory of Blood Safety Research of Liaoning Province | Li J.-P.,Key Laboratory of Blood Safety Research of Shenyang | Li J.-P.,Shenyang Research Center for Stem Cell Clinical Application
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: In recent years, with the development of China Marrow Donor Program and the improvement of human leukocyte antigen (HLA) typing technique, novel alleles of human leukocyte antigen have been discovered constantly in China. OBJECTIVE: To identify and confirm a novel HLA allele in a Chinese individual. METHODS: A new HLA allele was found during routine human leukocyte antigen genotyping by PCR-sequence specific oligonucleotide probes and sequencing-based typing. RESULTS AND CONCLUSION: The HLA-B locus hybridization probe reaction patterns of this sample did not match with any known HLA-B alleles or allelic combinations. Exons 2, 3 were sequenced in both directions using HLA-B sequence primer and group-specific sequencing primer. The obtained sequence had 2nts change from B*07:02:01 at nt 226 and nt 228 where A->G (codon 76 ATA->GTG) and resulting in a coding change, 76 isoleucine (I) was changed to valine (V). This nucleotide sequence has been submitted to the GenBank nucleotide sequence database and it is available under the accession number HM989017. A novel HLA allele, HLA-B*07:110, was identified, and was named officially by the WHO Nomenclature Committee. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All Rights Reserved. Source


Lin F.-Q.,Key Laboratory of Blood Safety Research of Liaoning | Lin F.-Q.,Key Laboratory of Blood Safety Research of Shenyang | Lin F.-Q.,Liaoning Blood Center | Li X.-F.,Key Laboratory of Blood Safety Research of Liaoning | And 12 more authors.
Tissue Antigens | Year: 2015

HLA-DRB1*11:106 has 1 nucleotide change from HLA-DRB1*11:01:01 at nucleotide 155 (G → A). © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd 85 1 January 2015 10.1111/tan.12483 New allele alerts New allele alerts © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Source


Zhang X.,Liaoning Blood Center | Li J.,Liaoning Blood Center | Li J.,Shenyang Research Center for Stem Cell Clinical Application
Chinese Journal of Medical Genetics | Year: 2015

Objective To explore the molecular basis for a rare Axl3B phenotype of the ABO subtype. Methods Serological assays were carried out to characterize the erythrocyte phenotype of the discrepant sample. Exons 6 and 7 of the ABO gene were amplified with polymerase chain reaction and subjected to direct sequencing. The amplicons were also cloned to separate the two alleles. Results Both A and B antigens were detected on the red blood cells of the proband, and anti-A antibody was detected in the serum. The serological phenotype of the sample was identified as AxB. DNA sequencing showed heterozygous status for 297AG, 526CG, 657CT, 703AG, 796AC, 803GC, 930GA and 940AG in exons 6 and 7. After cloning and sequencing, two alleles Axl3 and B101 were obtained. The sequence of Axl3 showed a nucleotide change(A to G) at position 940. Conclusion The 940A>G mutation of the α-1, 3-N-acetylgalactosaminyltransferase gene has resulted in the reduced expression of A antigen. Source


Li J.-P.,Key Laboratory of Blood Safety Research of Liaoning | Li J.-P.,Key Laboratory of Blood Safety Research of Shenyang | Li J.-P.,Liaoning Blood Center | Li J.-P.,Shenyang Research Center for Stem Cell Clinical Application | And 12 more authors.
Tissue Antigens | Year: 2014

HLA-B*55:46 has one nucleotide change from HLA-B*55:02:01 where 138N is changed to D. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Source


Li J.-P.,Liaoning Blood Center | Li J.-P.,Key Laboratory of Blood Safety Research of Liaoning | Li J.-P.,Key Laboratory of Blood Safety Research of Shenyang | Li J.-P.,Shenyang Research Center for Stem Cell Clinical Application | And 12 more authors.
Tissue Antigens | Year: 2013

B*07:110 has two nucleotides (nts) change from B*07:02:01 where 76 I is changed to V. © 2013 John Wiley & Sons A/S. Source

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