Wu B.,Orthopaedic Hospital of Shenyang |
Ma X.,Orthopaedic Hospital of Shenyang |
Zhu D.-M.,Orthopaedic Hospital of Shenyang |
Liu S.-Y.,Orthopaedic Hospital of Shenyang |
And 4 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: Studies have shown that insulin-like growth factor-1 serves as a helper factor and is combined combines with other factors to promote the differentiation of bone marrow mesenchymal stem cells into chondrocytes. However, whether insulin-like growth factor-1 alone could promote the secretion of cartilage specific collagen remains controversial, and its effect on cell proliferation and stability of cartilage collagenfiber has been rarely reported.OBJECTIVE: To investigate the effect of insulin-like growth factor-1 on chondrogenic differentiation and matrix metalloproteinase expression of bone marrow mesenchymal stem cells. METHODS: The expression vector carrying complete coding sequence of insulin-like growth factor-1 was constructed, and stably transfected to rat bone marrow mesenchymal stem cells, serving as insulin-like growth factor-1 stable transfection group. Meanwhile, the non-transfection group was taken as the control. RESULTS AND CONCLUSION: The 3-(4,5)-dimethylthiahiazo-2-yl-3,5-di-phenytetrazoliumromide assay showed that, insulin-like growth factor-1 stable expressing bone marrow mesenchymal stem cells was successfully established, and cell proliferation was significantly increased after 4 days of transfection (P < 0.05). Reverse transcription-polymerase chain reaction, western blot analysis and immunocytochemistry detection showed that, the mRNA and protein levels of insulin-like growth factor-1 and collagen type II were significantly increased in the stable transfection group (P < 0.01), while mRNA levels of matrix metalloproteinase-1, -2 and -3 were significantly decreased compared with the non-transfection group (P < 0.01). Experimental findings confirm that, insulin-like growth factor-1 alone can promote the proliferation of bone marrow mesenchymal stem cells as well as chondrogenic differentiation, and maintain the stability of collagen fibers.