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Sun W.,Northern Hospital of Shenyang | Pan L.,Nankai University | Ma Z.,Northern Hospital of Shenyang
Oligonucleotides | Year: 2010

To determine the mechanism of osmolarity involved in polyethylenimine (PEI)/oligonucleotide (ON) complex transfection in cells, we measured the fluorescence intensities of fluorescein isothiocyanate-labeled ONs complexed with PEI and the changes in cytosolic Ca2+ concentration ([Ca 2+]c) in A549 cells, and we found that uptake of PEI/ON complexes was improved in the cells along with a rise of [Ca2+] c in A549 cells challenged by 50% hypotonic medium. Further experiments showed that the enhanced uptake efficiency and the rise in [Ca 2+]c in A549 cells were almost completely abolished from cells loaded with the intracellular calcium chelator 1,2-bis(2-aminophenoxy)-N, N,N,N-tetraacetic acid-acetoxymethyl ester. 2-Aminoethoxydiphenyl borate or 8-(N,N-diethylamino) octyl-3,4,5-trimethoxybenzoate, two potent antagonists of inositol 1,4,5-trisphosphate-mediated Ca2+ release that blunt [Ca2+]c elevation via Ca2+ release from endoplasmic reticulum, inhibited the enhanced uptake of PEI/ON complexes induced by Ca2+-free hypo-osmotic stress. In summary, the results strongly suggest that calcium-dependent transfection is responsible for the uptake of PEI/ON complexes into A549 cells under hypotonic conditions. © Copyright 2010, Mary Ann Liebert, Inc. Source


Jiang H.,Northern Hospital of Shenyang | Wang H.S.,Northern Hospital of Shenyang | Wu H.B.,Northern Hospital of Shenyang | Li X.M.,Northern Hospital of Shenyang
Chirurgia (Turin) | Year: 2013

Bronchogenic cyst is considered as an uncommon congenital anomaly. It can be mostly found in mediastinum or lung. Intracardiac bronchogenic cyst is very rare. We found 2 cases in more than 20000 cardiac surgical cases in our department. The 2 cases bronchogenic cyst arose from interatrial septum (IAS), the preoperative diagnosis were myxoma, but the histological diagnosis were bronchogenic cyst in both cases. Although it is very rare, it should be considered while intracardiac tumor is diagnosed. It is recommended to complete resection of any bronchogenic cyst for primarily diagnostic and potentially therapeutic reasons, and every effort should be made to prevent complications due to injury to nearby tissues. Source


Jiang H.,Northern Hospital of Shenyang | Wang H.,Northern Hospital of Shenyang | Wang Z.,Northern Hospital of Shenyang | Zhu H.,Northern Hospital of Shenyang | Zhang R.,Northern Hospital of Shenyang
Journal of Cardiac Surgery | Year: 2013

Aims Common atrium (CA) is a rare congenital heart defect. We reviewed our experience of surgical treatment of CA and summarize the clinical features of CA and the key techniques for surgical correction. Methods Between August 1984 and August 2010, 37 consecutive cases of CA underwent corrective surgery. There were no clinical findings of Down, asplenia-polysplenia or Ellis-van Creveld syndromes in all cases. Mitral valvuloplasty was performed in 34 cases, and tricuspid valvuloplasty in 20 cases. Complete closure of a mitral cleft was required in 32 cases. All new atrial septa were reconstructed using patches. Follow-up period ranged from 1 to 20 years. Results There were no hospital deaths or conduction system block. After surgery, mild mitral insufficiency was observed in only one case. Two cases had moderate-severe mitral insufficiency at postoperative years 1 and 3, respectively, that required mitral valve replacements. One patient died of low cardiac output syndrome after reoperation. Conclusions Long-term survival after surgical correction of CA is good. Routine closure of a cleft in the mitral valve is very important for successful surgery. doi: 10.1111/jocs.12202 (J Card Surg 2013;28:580-586) © 2013 Wiley Periodicals, Inc. Source

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