Yuan B.,Shenyang Pharmaceutical University |
Yuan B.,Shenyang non clinical drug metabolism and pharmacokinetics engineering research center |
Liu C.,Shenyang Pharmaceutical University |
Liu C.,Shenyang non clinical drug metabolism and pharmacokinetics engineering research center |
And 9 more authors.
Biomedical Chromatography | Year: 2011
Coenzyme Q 10 (CoQ 10) is a naturally occurring compound located in all membranes throughout the cell. A rapid and sensitive HPLC method was developed to determine the concentration of CoQ 10 in dog plasma using a surrogate matrix. Chromatographic separation was carried out on a Diamonsil C 18 column with the UV detector set at 275nm. Methanol-2-propanol (40:60, v/v) was used as a mobile phase delivered at a flow rate of 1.0mL/min. Calibrators were prepared using blank plasma-K 2HPO 4 buffer (50mm, pH 8.0)-saline (1:3:6, v/v/v) as surrogate matrix. It was shown that the surrogate matrix had similar properties to dog plasma for CoQ 10 in extraction, freeze-thaw and stability. The assay was linear over the concentration range of 0.10-100μg/mL. The intra- and inter-day precisions were within 13.3% in terms of relative standard deviation (RSD%) and the accuracy was within ±7.5% in terms of relative error. This simple and reproducible HPLC method with less plasma volume (0.4mL) and adequate sensitivity was successfully applied to pharmacokinetic studies of CoQ 10 in dogs and an investigation of the effect of CoQ 10 formulation on CoQ 10 baseline levels. © 2010 John Wiley & Sons, Ltd.