Shenyang HB Optical Technology Co.

Shenyang, China

Shenyang HB Optical Technology Co.

Shenyang, China
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Zhao S.,Shenyang Academy of Instrumentation Science | Yin X.,Shenyang Academy of Instrumentation Science | Cao Y.,Shenyang HB Optical Technology Co. | Lei P.,Shenyang HB Optical Technology Co. | And 2 more authors.
Chinese Optics Letters | Year: 2013

Aiming at the crosstalk problem caused by small spectral intervals between fluorescent reagents in fourplex fluorescence quantitative polymerase chain reaction (PCR) detection analysis system, we calculate and analyse the effect of cut-off steepness, central wavelength positioning, and bandwidth of filters on crosstalk. Design and prepare four sets of fluorescence excitation and emission filters with proper shape cut-off steepness (optical density (OD) from OD 0.3 to OD 6 is less than 8 nm) and bandwidth (9-11 nm). In fourplex PCR instrument, crosstalk coefficient in all four channels are less than 0.3%. © 2013 Chinese Optics Letters.


Yin X.,Shenyang Academy of Instrumentation Science | Zhao S.,Shenyang Academy of Instrumentation Science | Fei S.,Shenyang Academy of Instrumentation Science | Gao P.,Shenyang Academy of Instrumentation Science | And 4 more authors.
Chinese Optics Letters | Year: 2010

Plasma ion-assisted deposition (PIAD) process for ultraviolet (UV)-induced transmission and full dielectric thin-film filters in the 200-400 nm spectral region is described. The design and manufacturing method of the UV filters are introduced. The UV filters exhibit deep blocking (>optical density (OD)5-OD6), high transmittance, and stable environment durability. These UV filters pass 10 cycles in an aggravated temperature-humidity test, according to ISO9022-2 and MIL-STD-810F standards. © 2010 Chinese Optics Letters.

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