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Hao D.,Liaoning Medical University | Zhang J.,Shenyang First Peoples Hospital
World Chinese Journal of Digestology | Year: 2011

AIM: To observe the effect of body position on respiratory mechanics in patients during laparoscopic surgery. METHODS: Reserve trendelenburg position was adopted in 20 patients undergoing laparoscopic gastrectomy, while conventional trendelenburg position was adopted in 20 patients undergoing laparoscopic enterectomy. All patients were subjected to general anaesthesia, and controlled ventilation was selected. The compliance of the lungs (Cpat) and airway pressure (Paw) were monitored with the Drager primus anesthesia machine. PaCO2 and PaO2 were monitored with the Siemens Rapidlab1265 Blood Gas Analyzer. Measurements were divided into five distinct phases: 5 minutes after the induction of anesthesia (T1); 5 minutes after pneumoperitoneum (T2); 5 minutes after position change (T3); 5 minutes after adjusting ventilation parameters (T4); and 5 minutes after peritoneal deflation (T5). Respiratory mechanics were analyzed using SPSS15.0 statistics software. RESULTS: PaCO2 and Paw at T2 in the conventional trendelenburg group were significantly higher than those in the reserve trendelenburg group (both P < 0.05). PaO2 and Cpat at T2 and T3 in the conventional trendelenburg group were significantly lower than those in the reserve trendelenburg group (all P < 0.01). CONCLUSION: Body position can affect respiratory parameters during laparoscopic surgery.

Bauquier S.H.,University of Melbourne | Lai A.,University of Melbourne | Jiang J.L.,St. Vincents Hospital | Jiang J.L.,University of Melbourne | And 3 more authors.
Neuroscience Bulletin | Year: 2015

The aim of this prospective blinded study was to evaluate an automated algorithm for spike-and-wave discharge (SWD) detection applied to EEGs from genetic absence epilepsy rats from Strasbourg (GAERS). Five GAERS underwent four sessions of 20-min EEG recording. Each EEG was manually analyzed for SWDs longer than one second by two investigators and automatically using an algorithm developed in MATLAB®. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated for the manual (reference) versus the automatic (test) methods. The results showed that the algorithm had specificity, sensitivity, PPV and NPV >94%, comparable to published methods that are based on analyzing EEG changes in the frequency domain. This provides a good alternative as a method designed to mimic human manual marking in the time domain. © 2015, Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg.

Jiang J.L.,University of Melbourne | Jiang J.L.,Center for Clinical Neurosciences and Neurological Research | Yue Z.,University of Wollongong | Bauquier S.H.,University of Melbourne | And 10 more authors.
Restorative Neurology and Neuroscience | Year: 2015

Purpose: Epilepsy is a prevalent neurological disorder with a high frequency of drug resistance. While significant advancements have been made in drug delivery systems to overcome anti-epileptic drug resistance, efficacies of materials in biological systems have been poorly studied. The purpose of the study was to evaluate the anti-epileptic effects of injectable poly(epsiloncaprolactone) (PCL) microspheres for controlled release of an anticonvulsant, phenytoin (PHT), in an animal model of epilepsy. Methods: PHT-PCL and Blank-PCL microspheres formulated using an oil-in-water (O/W) emulsion solvent evaporation method were evaluated for particle size, encapsulation efficiency, surface morphology and in-vitro drug release profile. Microspheres with the most suitable morphology and release characteristics were subsequently injected into the hippocampus of a rat tetanus toxin model of temporal lobe epilepsy. Electrocorticography (ECoG)from the cerebral cortex were recorded for all animals. The number of seizure events, severity of seizures, and seizure duration were then compared between the two treatment groups. Results: We have shown that small injections of drug-loaded microspheres are biologically tolerated and released PHT can control seizures for the expected period of time that is in accord with in-vitro release data. Conclusion: The study demonstrated the feasibility of polymer-based delivery systems in controlling focal seizures. © 2015 - IOS Press and the authors. All rights reserved.

Li L.,Liaoning Medical University | Wang J.,Chinese Peoples Liberation Army | Tang L.,Liaoning Medical University | Yu X.,Liaoning Medical University | And 2 more authors.
Molecular Medicine Reports | Year: 2015

Bone marrow stromal cells (BMSCs), derived from the mesoderm, have been applied in the repair and reconstruction of injured tissues. The present study was conducted to explore the effects of BMSCs on cell viability of tumor necrosis factor-α (TNF-α)-stimulated PC12 cells. PC12 cells were co-cultured with BMSCs under TNF-α treatment, with normal PC12 cells as controls. Results from an MTT assay indicated that BMSCs significantly increased cell growth and proliferation of TNF-α-treated PC12 cells (survival rates were 56.71 and 76.86% for the positive control (PC) and co-culture group, respectively). Furthermore, Annexin V/propidium iodide staining and flow cytometric analysis demonstrated that TNF-α increased PC12-cell apoptosis from 3.49 to 40.74% in the negative control and PC group, and the apoptotic rate was significantly reduced upon co-culture with BMSCs to 16.97%. In addition, data from reverse transcription-quantitative polymerase chain reaction and western blot analyses illustrated that TNF-α-induced upregulation in TNF receptor (TNFR)-1 (TNFR1) and caspase-8 expression in PC12 cells were partially reversed by co-culture with BMSCs. In conclusion, the present study suggested that BMSCs protect PC12 cells against stimulation with TNF-α, which is partially mediated through the TNFR/caspase signaling pathway. The results of the present study also suggested a therapeutic use of BMSCs in clinical neurodegenerative diseases.

Zhang X.,Shenyang First Peoples Hospital | Hu W.,Liaoning Medical University | Feng F.,Liaoning University of Traditional Chinese Medicine | Xu J.,Shenyang First Peoples Hospital | Wu F.,Shenyang First Peoples Hospital
Molecular Medicine Reports | Year: 2016

Myocardial infarction is a serious health threat. Apelin is an endogenous ligand of angiotensin II receptor-like 1 (APJ) and the apelin/APJ system is associated with various types of heart disease. However, whether apelin protects against myocardial infarction-induced myocardial fibrosis remains unclear. The present study aimed to investigate the function of apelin-13 during myocardial infarction-induced myocardial fibrosis, and to determine the mechanism underlying the effects of apelin-13. Apelin-13 was demonstrated to improve left ventricular function and results of hematoxylin and eosin staining, Masson's trichrome staining and western blotting showed that apelin-13 attenuated myocardial fibrosis. Further mechanistic investigation was performed by enzyme-linked immunosorbent assay, western blotting and electrophoretic mobility shift assay. The results demonstrated that apelin-13 inhibited the activation of nuclear factor (NF)-κB signaling in vitro and in vivo. To the best of our knowledge, the present study was the first to demonstrate that apelin-13 may attenuate myocardial infarction-induced myocardial fibrosis, and that this protective function may be mediated by inhibition of NF-κB signaling. The present study suggests a theoretical basis for the effects of apelin-13 and provides insight into the potential clinical application of apelin-13.

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