Shenyang Entry Exit Inspection and Quarantine Bureau

Shenyang, China

Shenyang Entry Exit Inspection and Quarantine Bureau

Shenyang, China

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Li H.,Qingdao Agricultural University | Lang K.-L.,Qingdao Agricultural University | Fu H.-B.,Shenyang Entry Exit Inspection and Quarantine Bureau | Shen C.-P.,Qingdao Agricultural University | And 3 more authors.
Insect Science | Year: 2015

The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution. © 2015 Institute of Zoology, Chinese Academy of Sciences.


Jiang B.,Northeastern University China | Zhao Y.,Northeastern University China | Jin Y.,Northeastern University China | Hu X.,Northeastern University China | And 2 more authors.
Advanced Materials Research | Year: 2012

In study, Microwave Irradiation enhanced Fenton-Like Process was introduced as a novel technology in the treatment of Landfill Leachate containing refractory organic matters. The sample leachate with initial COD loading of 11434 ∼12067 mg L -1 was collected from the Laohuchong (Shenyang, China) City Landfill. Fenton (FeSO 4/H 2O 2) and Fenton-like (iron tailings/H 2O 2) reagents coupling with Microwave Irradiation were both studied. The Microwave Irradiation enhanced Fenton-Like Process was conducted under the optimal conditions as follows: 480W of microwave power, 3 min of radiation time, 3.0 of initial pH, 100mL L -1 of H 2O 2 and 2g L -1 of iron tailings. The removal rates of leachate samples with initial COD =11928 mg L -1 using 1.5g.L -1 FeSO 4 and 2.0g.L -1 iron tailings are 45.1% and 42.3% respectively on the condition of 320W MW radiated for 3 min at room temperature. The highest removal rate of the MW Fenton-Like Process is about 46% under the optimum conditions. It demonstrates the Fenton-Like reagent can be used as a substitution of Fenton reagent in Landfill Leachate applications which has extensive application prospect and extraordinary significance in aspects of resource utilization of waste materials and cost saving.


Geng Q.,Shenyang Entry Exit Inspection and Quarantine Bureau | Ding J.,Dalian Ocean University | Jiang L.,Shenyang Entry Exit Inspection and Quarantine Bureau | Wu Y.,Dalian Ocean University | Bao P.,Dalian Ocean University
Energy Education Science and Technology Part A: Energy Science and Research | Year: 2014

The main purpose of this study is to study the toxic effect of short-term nonionic surfactants dibenzyl phenol polyoxyethylene ether (BP) and fatty alcohol polyoxyethylene ether (AEO) exposure on the zebra fish Danio rerio. After treatment with different concentrations (0 mg/L, 0.25 mg/L, 0.5 mg/L, 1 mg/L, 2 mg/L) of BP and AEO for 30 days, the catalase (CAT) and superoxide dismutase (SOD) activities increased while the glutathione (GSH) content decreased in liver tissue of experimental zebrafish, and no significant different changes were observed in levels of malondialdehyde (MDA) and total antioxidant capacity (T-AOC). These results indicated the nonionic surfactants BP and AEO had potential toxic effects on zabrafish antioxidant defense system, and supported the view that a relationship exists between pollution and immunomodulation in aquatic organisms. © Sila Science. All Rights Reserved.


Jiang B.H.,Northeastern University China | Jiang L.,Shenyang Entry Exit Inspection and Quarantine Bureau | Fu L.L.,Northeastern University China | Fu L.L.,Liaoning University | And 3 more authors.
Advanced Materials Research | Year: 2013

The distribution and accumulation of heavy metals in the planting base soils in Shenyang, northeast of China, were investigated. Sixty soil samples were collected and characterized for heavy metals contents (As, Cd, Cr, Pb, Hg, Ni, Cu and Zn). Concentration data were processed using correlation analysis and soils pollution assessment was carried out using geoaccumulation and PI. The results revealed that eight heavy metals contents of planting base all reached pollution level, which Pb and Hg pollution relatively serious, reached 2.41 and 2.1 respectively. The order of heavy metal pollution levels are: Pb> Hg> Zn = Cu> As> Ni> Cd> Cr. A significant correlation exists in Pb-Cd, Pb-Cu, Cd-As, Cu-Ni, while an extremely significant correlation exists between Zn-Cu. In addition to the parent material soil, the sources of soil heavy metals are mainly by the industrial, agricultural, transportation or other human activities and the effect of atmospheric transport and deposition. © (2013) Trans Tech Publications, Switzerland.


Jiang B.,Northeastern University China | Meng F.,Northeastern University China | Ma T.,Northeastern University China | Jiang L.,Shenyang Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings | Year: 2011

To address the high cost and low efficiency in treatment of refractory organics in landfill leachate, the treatment efficiency of landfill leachate by microwave-enhanced adsorption and Fenton oxidation was studied. Landfill leachate with high concentration COD and ammonia nitrogen (initial COD and ammonia nitrogen concentration were 33384.17mg/L and 3588.16 mg/L.), which were difficult to be biodegraded directly. Based on species and dosage of adsorbent and oxidant, pH, microwave intensity and duration, a series of experimental studies were carried on, concluding that absorption and oxidation enhanced by microwave process using zeolite (15 mesh) as adsorbent and Fenton reagent as oxidant, the dosage of zeolite was 40g/L, pH 3, the best dosage of Fenton reagent was 1g/L FeSO4 and 0.1L/LH2O2, microwave intensity was 640W, microwave initial temperature was 80°C. In order to improve the treatment efficiency, flocculation and sand filtration treatment was settled to treat landfill leachate before this process, mainly to remove suspended solids and colloidal wastewater in substance and to clarify the water. Under optimal conditions, through absorption and oxidation enhanced by microwave treatment, the removal rate of COD and ammonia nitrogen (making the concentration after filtration as the initial concentration) were 51.60% and 76.82%. The mechanism findings indicated: Adsorption and oxidation worked meanwhile, the skeleton-like crystal structure of zeolite provides oxidation carrier for oxidants, which improved the efficiency of the oxidation. Microwave not only decomposed the refractory organic matter and strengthened the zeolite dispersion force and electrostatic adsorption of the role of ammonia, but also activated H2O2 to produce hydroxyl radical to oxidize wastewater with Fenton's reagent. Through microwave-enhanced absorption and oxidation process, foul odor of landfill leachate disappeared, the color changed from thick black to colorless, concentration of organic matter and ammonia nitrogen decreased significantly. © 2011 IEEE.


Jiang B.-H.,Shenyang University | Jiang L.,Shenyang Entry Exit Inspection and Quarantine Bureau | Li Z.-M.,Shenyang University | Kang K.,Shenyang Entry Exit Inspection and Quarantine Bureau | Li R.-N.,Shenyang University
Applied Mechanics and Materials | Year: 2012

After initial screening on enrichment media, with subsequent sequential isolation on minimal media containing the cypermethrin as sole C source, three degrading strains were isolated from cypermethrin polluted soil. Gas chromatography was used to measure the rates of disappearance of the cypermethrin, the strain L0 was identified as Bacillius sp. preliminarily. The optimum growth conditions for the aerobic degradation of cypermethrin by L0 were 26°C, pH 7. The degrade rate of cypermethrin by L0 is more than 87.54% above conditions after cultivated for 72 hours. Strain L0 is a high effect degradation bacterium should be used for a bioremediation process of the contaminated soils by insecticides and decrease the environmental pollution. © (2012) Trans Tech Publications, Switzerland.


Zhang M.,ShenYang Agricultural University | Li J.Y.,ShenYang Agricultural University | Fu H.B.,Shenyang Entry exit Inspection and Quarantine Bureau
Applied Mechanics and Materials | Year: 2014

To study the saccharification conditions of cellulase on lignocellulose material, this paper used corn straw as the material to do research on the effect of cellulase. The hydrolysis factors, including reaction temperature, reaction time and cellulase concentration, were measured in order to determine the optimal saccharification conditions for fuel ethanol production. The results showed that, when the reaction temperature was 45°C, reaction time was 72 h and cellulase concentration was 60 IU/g, the hydrolysis rate can respectively reach 6.25%, 14.7% and 14.4%. By the analysis of orthogonal test, the main factors effects the saccharification was reaction time. And the optimal combination of saccharification conditions was the reaction time of 54 h, the reaction temperature of 45 °C, and the cellulase concentration of 55 IU/g. © (2014) Trans Tech Publications, Switzerland.


Zhang W.-M.,Liaoning Provincial Plant Protection Station | Fu H.-B.,Shenyang Entry Exit Inspection and Quarantine Bureau | Wang W.-H.,Liaoning Provincial Plant Protection Station | Piao C.-S.,Liaoning Provincial Plant Protection Station | And 3 more authors.
Journal of Economic Entomology | Year: 2014

In Liaoning Province, China, tomato yellow leaf curl virus (TYLCV) was first detected in 2009 and in only four counties. To quantify the spread of TYLCV and to identify potential factors influencing its spread in Liaoning Province, we assayed for TYLCV within 1,055 whiteflies (Bemisia tabaci (Gennadius) complex) from 74 populations and 29 counties in 2011. The B. tabaci species of these individuals was determined based on molecular markers. TYLCV was found in 13 counties (Donggang, Liaoyang, Kazuo, Lingyuan, Heishan, Liaozhong, Kaiyuan, Taian, Dawa, Dashiqiao, Beizhen, Linghai, and Xingcheng) and was most frequently detected in the central plain. In addition, the percentage of whiteflies with TYLCV was significantly higher in B. tabaci Q than in B. tabaci B but was unrelated to the hosts (pepper, eggplant, tomato, cucumber, and kidney bean) on which the whiteflies had been collected. These results demonstrate that TYLCV has spread rapidly in Liaoning Province since its first detection and suggest that its spread is more closely associated with the introduction of B. tabaci Q than with the species of host plant. These findings also indicate that controls are now needed to reduce the further spread of TYLCV and that these controls should include the management of B. tabaci Q populations. © 2014 Entomological Society of America.


Li J.,Shenyang Entry Exit Inspection and Quarantine Bureau | Ying Z.,Shenyang Entry Exit Inspection and Quarantine Bureau | Rui H.,Shenyang Entry Exit Inspection and Quarantine Bureau | Wei P.,Shenyang Entry Exit Inspection and Quarantine Bureau | And 4 more authors.
Proceedings - 4th International Conference on Intelligent Computation Technology and Automation, ICICTA 2011 | Year: 2011

Organ phosphorus pesticides (OPs) were first synthesized in large quantities in the 1940's and are now widely used in China. Although OPs can degrade rapidly, they are also highly toxic. Indeed, many cases of poisoning in China, especially from vegetable sources, were attributed to the presence of OPs in large quantities. Even though the use of highly toxic pesticides in vegetables has been banned in China, in recent years high residual amounts of pesticides (mainly parathion and methamidophos) have been found in vegetable samples through routine monitoring efforts conducted by the Ministry of Agriculture. Hundreds of cases of acute poisoning resulting from the contamination of agricultural products with pesticides are now reported every year, posing a serious threat to the health of consumers and simultaneously leading to an increased level of awareness of the issue. In this study, we examine pesticide residue levels in vegetable samples (cucumber, celery, tomato, green pepper and eggplant) from four sources in Shenyang City. Our results show that OP levels in cucumber, celery and tomato samples were above the safe limits for consumption. Overall, samples from the larger supermarkets of Shenyang were safer than those from retailers and from the farmers' market. Parathion was the most commonly detected residue. Omethoate, phorate and methyl parathion, currently prohibited due to their highly toxic nature, were also detected in some samples. © 2011 IEEE.


PubMed | Shenyang Entry Exit Inspection and Quarantine Bureau and Qingdao Agricultural University
Type: Journal Article | Journal: Insect science | Year: 2016

The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.

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