Shenyang Entry Exit Inspection and Quarantine Bureau

Shenyang, China

Shenyang Entry Exit Inspection and Quarantine Bureau

Shenyang, China
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Liu H.,Jiangsu University | He Y.,Guangzhou University of Chinese Medicine | Zhaoying,Shenyang Entry Exit Inspection and Quarantine Bureau | Ke P.,University of London
African Journal of Pharmacy and Pharmacology | Year: 2011

The aim of this article was to fabricate novel ambroxol hydrochloride carboxymethyl chitosan microspheres without burst release. Firstly the blank carboxymethyl chitosan microspheres were fabricated by the emulsion chemical cross-linking method and the blank microspheres showed a controllable biodegradation in vitro in lysozyme solution. Then the ambroxol hydrochloride microspheres were prepared by the column method. The type of bonding between ambroxol hydrochloride and carboxymethyl chitosan microspheres was investigated by X-ray diffraction; the results showed that the drug chemically bonds to the ion exchangeable structure of the microspheres. The evaluation of the microspheres was investigated by dynamic light scattering, scanning electron microscopy (SEM) and UV spectrophotometer. The microspheres were spherical and consistent, and had an average diameter of 7.4 μm, the drug content of the microspheres was 15.3±0.7% (w/w). Finally the in vitro drug release was tested in different ionic concentration dissolution mediums. The results showed that the microspheres had a sustained-release profile for 8 h in vitro without obvious burst release. © 2011 Academic Journals.


Liu H.,Jiangsu University | Bao P.,Jiangsu University | Xu Y.,Shenyang Entry Exit Inspection and Quarantine Bureau | Shi S.,Jiangsu University | And 4 more authors.
Latin American Journal of Pharmacy | Year: 2017

Poly (lactide-co-glycolide) (PLGA) biodegradable microspheres is a promising method of sustained-release in protein/peptide delivery systems. But protein/peptide incorporated in PLGA microsphere were often exposed to unfavorable microenvironments with hydrophobicity and acidity. Bioactive glass is a new biodegradable carrier material, which can form alkaline substances gradually in vivo release. It can be used to neutralize the acidity caused by degradation of PLGA, stabilize the protein in the drug release process. In this study, a novel exenatide loaded bioactive glass (BG)-PLGA microsphere were prepared using an S/W/O/W double emulsion and solvent evaporation method, which were spherical in shape, and showed a smooth surface and a high encapsulation efficiency (> 70%). From the in vitro release experiment, the exenatide was controlled to release over 35 days. Furthermore, a long-lasting effect on blood glucose controlling was demonstrated. This study demonstrated the potential of the sustained release formulation for protein/peptide drugs. © 2017, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.


Li H.,Qingdao Agricultural University | Lang K.-L.,Qingdao Agricultural University | Fu H.-B.,Shenyang Entry Exit Inspection and Quarantine Bureau | Shen C.-P.,Qingdao Agricultural University | And 3 more authors.
Insect Science | Year: 2015

The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution. © 2015 Institute of Zoology, Chinese Academy of Sciences.


Jiang B.,Northeastern University China | Zhao Y.,Northeastern University China | Jin Y.,Northeastern University China | Hu X.,Northeastern University China | And 2 more authors.
Advanced Materials Research | Year: 2012

In study, Microwave Irradiation enhanced Fenton-Like Process was introduced as a novel technology in the treatment of Landfill Leachate containing refractory organic matters. The sample leachate with initial COD loading of 11434 ∼12067 mg L -1 was collected from the Laohuchong (Shenyang, China) City Landfill. Fenton (FeSO 4/H 2O 2) and Fenton-like (iron tailings/H 2O 2) reagents coupling with Microwave Irradiation were both studied. The Microwave Irradiation enhanced Fenton-Like Process was conducted under the optimal conditions as follows: 480W of microwave power, 3 min of radiation time, 3.0 of initial pH, 100mL L -1 of H 2O 2 and 2g L -1 of iron tailings. The removal rates of leachate samples with initial COD =11928 mg L -1 using 1.5g.L -1 FeSO 4 and 2.0g.L -1 iron tailings are 45.1% and 42.3% respectively on the condition of 320W MW radiated for 3 min at room temperature. The highest removal rate of the MW Fenton-Like Process is about 46% under the optimum conditions. It demonstrates the Fenton-Like reagent can be used as a substitution of Fenton reagent in Landfill Leachate applications which has extensive application prospect and extraordinary significance in aspects of resource utilization of waste materials and cost saving.


Geng Q.,Shenyang Entry Exit Inspection and Quarantine Bureau | Ding J.,Dalian Ocean University | Jiang L.,Shenyang Entry Exit Inspection and Quarantine Bureau | Wu Y.,Dalian Ocean University | Bao P.,Dalian Ocean University
Energy Education Science and Technology Part A: Energy Science and Research | Year: 2014

The main purpose of this study is to study the toxic effect of short-term nonionic surfactants dibenzyl phenol polyoxyethylene ether (BP) and fatty alcohol polyoxyethylene ether (AEO) exposure on the zebra fish Danio rerio. After treatment with different concentrations (0 mg/L, 0.25 mg/L, 0.5 mg/L, 1 mg/L, 2 mg/L) of BP and AEO for 30 days, the catalase (CAT) and superoxide dismutase (SOD) activities increased while the glutathione (GSH) content decreased in liver tissue of experimental zebrafish, and no significant different changes were observed in levels of malondialdehyde (MDA) and total antioxidant capacity (T-AOC). These results indicated the nonionic surfactants BP and AEO had potential toxic effects on zabrafish antioxidant defense system, and supported the view that a relationship exists between pollution and immunomodulation in aquatic organisms. © Sila Science. All Rights Reserved.


Jiang B.H.,Northeastern University China | Jiang L.,Shenyang Entry Exit Inspection and Quarantine Bureau | Fu L.L.,Northeastern University China | Fu L.L.,Liaoning University | And 3 more authors.
Advanced Materials Research | Year: 2013

The distribution and accumulation of heavy metals in the planting base soils in Shenyang, northeast of China, were investigated. Sixty soil samples were collected and characterized for heavy metals contents (As, Cd, Cr, Pb, Hg, Ni, Cu and Zn). Concentration data were processed using correlation analysis and soils pollution assessment was carried out using geoaccumulation and PI. The results revealed that eight heavy metals contents of planting base all reached pollution level, which Pb and Hg pollution relatively serious, reached 2.41 and 2.1 respectively. The order of heavy metal pollution levels are: Pb> Hg> Zn = Cu> As> Ni> Cd> Cr. A significant correlation exists in Pb-Cd, Pb-Cu, Cd-As, Cu-Ni, while an extremely significant correlation exists between Zn-Cu. In addition to the parent material soil, the sources of soil heavy metals are mainly by the industrial, agricultural, transportation or other human activities and the effect of atmospheric transport and deposition. © (2013) Trans Tech Publications, Switzerland.


Jiang B.-H.,Shenyang University | Jiang L.,Shenyang Entry Exit Inspection and Quarantine Bureau | Li Z.-M.,Shenyang University | Kang K.,Shenyang Entry Exit Inspection and Quarantine Bureau | Li R.-N.,Shenyang University
Applied Mechanics and Materials | Year: 2012

After initial screening on enrichment media, with subsequent sequential isolation on minimal media containing the cypermethrin as sole C source, three degrading strains were isolated from cypermethrin polluted soil. Gas chromatography was used to measure the rates of disappearance of the cypermethrin, the strain L0 was identified as Bacillius sp. preliminarily. The optimum growth conditions for the aerobic degradation of cypermethrin by L0 were 26°C, pH 7. The degrade rate of cypermethrin by L0 is more than 87.54% above conditions after cultivated for 72 hours. Strain L0 is a high effect degradation bacterium should be used for a bioremediation process of the contaminated soils by insecticides and decrease the environmental pollution. © (2012) Trans Tech Publications, Switzerland.


Zhang M.,ShenYang Agricultural University | Li J.Y.,ShenYang Agricultural University | Fu H.B.,Shenyang Entry exit Inspection and Quarantine Bureau
Applied Mechanics and Materials | Year: 2014

To study the saccharification conditions of cellulase on lignocellulose material, this paper used corn straw as the material to do research on the effect of cellulase. The hydrolysis factors, including reaction temperature, reaction time and cellulase concentration, were measured in order to determine the optimal saccharification conditions for fuel ethanol production. The results showed that, when the reaction temperature was 45°C, reaction time was 72 h and cellulase concentration was 60 IU/g, the hydrolysis rate can respectively reach 6.25%, 14.7% and 14.4%. By the analysis of orthogonal test, the main factors effects the saccharification was reaction time. And the optimal combination of saccharification conditions was the reaction time of 54 h, the reaction temperature of 45 °C, and the cellulase concentration of 55 IU/g. © (2014) Trans Tech Publications, Switzerland.


Zhang W.-M.,Liaoning Provincial Plant Protection Station | Fu H.-B.,Shenyang Entry Exit Inspection and Quarantine Bureau | Wang W.-H.,Liaoning Provincial Plant Protection Station | Piao C.-S.,Liaoning Provincial Plant Protection Station | And 3 more authors.
Journal of Economic Entomology | Year: 2014

In Liaoning Province, China, tomato yellow leaf curl virus (TYLCV) was first detected in 2009 and in only four counties. To quantify the spread of TYLCV and to identify potential factors influencing its spread in Liaoning Province, we assayed for TYLCV within 1,055 whiteflies (Bemisia tabaci (Gennadius) complex) from 74 populations and 29 counties in 2011. The B. tabaci species of these individuals was determined based on molecular markers. TYLCV was found in 13 counties (Donggang, Liaoyang, Kazuo, Lingyuan, Heishan, Liaozhong, Kaiyuan, Taian, Dawa, Dashiqiao, Beizhen, Linghai, and Xingcheng) and was most frequently detected in the central plain. In addition, the percentage of whiteflies with TYLCV was significantly higher in B. tabaci Q than in B. tabaci B but was unrelated to the hosts (pepper, eggplant, tomato, cucumber, and kidney bean) on which the whiteflies had been collected. These results demonstrate that TYLCV has spread rapidly in Liaoning Province since its first detection and suggest that its spread is more closely associated with the introduction of B. tabaci Q than with the species of host plant. These findings also indicate that controls are now needed to reduce the further spread of TYLCV and that these controls should include the management of B. tabaci Q populations. © 2014 Entomological Society of America.


PubMed | Shenyang Entry Exit Inspection and Quarantine Bureau and Qingdao Agricultural University
Type: Journal Article | Journal: Insect science | Year: 2016

The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.

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