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Chen X.,Shengli Oilfield Central Hospital | Lyu Z.,Shengli Oilfield Central Hospital | Gao Z.,Shengli Oilfield Central Hospital
Chinese Journal of Cerebrovascular Diseases | Year: 2016

Objective: To evaluate the effect of using different doses of contrast agent on renal function in patients with ischemic cerebrovascular disease who received endovascular interventional treatment. Methods: A total of 82 consecutive patients with ischemic cerebrovascular disease treated with interventional therapy at the Department of Neurology, Shengli Oilfield Central Hospital from March 2013 to March 2014 were enrolled retrospectively. The gender, age, body mass of the patients, and whether having the history of hypertension or diabetes were documented. According to the dose of contrast agent in the angiography and treatment, they were divided into either a low-dose group (iodine 240 mg I/ml <150ml; n = 44) or a high-dose group (≥ 150ml; n =38). The serum cystatin (CysC), urinary micro-albumin (mAlb), and serum retinol binding protein (RBP) levels were detected at day 1 before and after procedure. The changes of the above 3 indices were compared. The effect of the dosage of contrast agent on renal function was analyzed. Results: (1) Compared with before procedure, there were significant differences in CysC and mAlb after procedure in the low-dose group (Z = 4.231; P < 0.05; Z = 2.220, P < 0.05). (2) There were significant differences in RBP, CysC and mAlb in the high-dose group before and after procedures (Z =3.872, P < 0.05; Z =3.077, P < 0.05; Z = 5.377, P < 0.05). (3) There were significant differences in RBP and mAlb before and after procedure between the 2 groups (Z = -3.167, P < 0.05; Z = - 6.823, P < 0.05). There were no significant differences in CysC before and after procedure between the 2 groups (Z = -0.671, P > 0.05). Conclusion: The dosage of contrast agent was associated with the decreased renal function after interventional therapy of ischemic cerebrovascular disease. The greater the dosage of contrast agent, the higher the renal function damage.


Gao Z.,Shengli Oilfield Central Hospital
Chinese Journal of Cerebrovascular Diseases | Year: 2017

Objective: To investigate the effectiveness and safety in patients with largeartery occlusive acute cerebral infarction who received multi-interventional modes mainly with mechanical thrombectomy and its related factors affecting prognosis. Methods: The clinical data of 56 patients with large artery occlusive acute cerebral infarction were analyzed retrospectively. The clinical characteristics (gender, age, and underlying diseases), timing of treatment (time from ictus to puncture, time from puncture to recanalization), multi-interventional mode therapies (intra-arterial thrombolysis, thrombectomy, balloon dilation, and stenting, etc.), and distribution of offending vessels were observed. The modified Thrombolysis in Cerebral Ischemia Scale (mTICI) grade was used to evaluate revascularization. The National Institute of Health Stroke Scale (NIHSS) score was used to observe the neurological function at 24 h before and after procedures. The modified Rankin scale (mRS) was used to evaluate the prognosis at 3 months after procedure. The safety of the treatment was evaluated with operative complications (mainly symptomatic intracranial hemorrhage) and mortality. The patients were divided into either a good prognosis group (n = 34;mRS≤2) or a poor prognosis group (n = 22;mRS≥3) according to the prognosis at 3 months after procedure. They were analyzed with univariate analysis. The factors influencing the prognosis were further analyzed with multivariate logistic regression analysis. Results: (1) The recanalization rate in 56 patients was 78.6% (n = 44), in which basilar artery was the highest, reaching 93.8% (15/16), middle cerebral artery was 87.0% (20/23). The NIHSS score at 24 hours was 10 ± 7, it was lower than 16 ± 6 on admission. There was significant difference (t = 6.401, P <0.01). At 3 months, 34 patients (60.7%) had good prognosis, 4 (7.1%) died, and 8 (14.3%) had symptomatic intracranial hemorrhage. (2) Multiple factor analysis showed that the high level of recanalization was a protective factor for good prognosis (OR, 0.465, 95% CI 0.267 -0.809, P = 0.007). Diabetes was an independent risk factor for poor prognosis (OR, 5.535, 95% CI 1.101 -27.835, P = 0.038). Conclusion: Acute large artery occlusive cerebral infarction treated with the intra-arterial multi-interventional modes may quickly and effectively restore intracranial blood flow. It has the characteristics of high recanalization rate and good prognosis, and the higher the level of recanalization, the better the prognosis. Diabetes is an independent risk factor for poor prognosis. © 2017, Society of China University Journals in Natural Sciences. All rights reserved.


Shi J.,Shengli Oilfield Central Hospital | Shi J.,Anhui Medical University | Li W.,Anhui Medical University | Ding X.,Shengli Oilfield Central Hospital
International Journal of Biological Markers | Year: 2017

Background:The protein encoded by ZBTB20 is a member of the POK family, whose members function as transcriptional repressors through interactions mediated by their conserved C2H2 Krüppel-type zinc finger and BTB/POZ domains. Polymorphisms in ZBTB20 appeared to be associated with gastric and esophageal cancer susceptibility in biological models, but the results of these studies were inconclusive. Therefore, we conducted a meta-analysis by pooling all available data to assess the exact association between the ZBTB20 rs9841504 polymorphism and gastric and esophageal cancer susceptibility. Method: The meta-analysis was performed for homozygote comparison, heterozygote comparison, and dominant and recessive models by applying a fixed- or random-effects model. The pooled odds ratios (ORs) with the corresponding confidence intervals (CIs) were calculated. Moreover, the data were analyzed using the Stata 12.0 software(StataCorp). Result: A total of 8 independent case-control studies comprising 9,994 cases and 10,258 controls were included. We found a significant association between the rs9841504 polymorphism and decreased gastric cancer susceptibility in the allelic, homozygous, dominant and recessive models (B vs. A:OR = 0.797, 95% CI 0.644-0.986, p = 0.036; BB vs. AA:OR = 0.601, 95% CI 0.366-0.988, p = 0.045; BA + BB vs. AA:OR = 0.789, 95% CI 0.627-0.992, p = 0.043; BB vs. BA + AA:OR = 0.635, 95% CI 0.405-0.997, p = 0.049). Conversely, no association between the rs9841504 polymorphism and esophageal cancer susceptibility was found. In subgroup analysis by ethnicity, we observed a significantly decreased susceptibility to gastric cancer in Asian populations in the allele contrast, homozygous and recessive models (B vs. A:OR = 0.791, 95% CI 0.628-0.996, p = 0.046; BB vs. AA:OR = 0.559, 95% CI 0.323-0.966, p = 0.037; BB vs. BA + AA:OR = 0.593, 95% CI 0.361-0.972, p = 0.038). Conclusions: In summary, our work suggests that the ZBTB20 rs9841504 polymorphism is a protective factor for gastric cancer rather than esophageal cancer. © 2016 Wichtig Publishing.


Xu L.,Shengli Oilfield Central Hospital | Xu D.-Z.,Shengli Oilfield Central Hospital
Chinese Journal of Medical Imaging Technology | Year: 2015

Objective: To explore MRI manifestations of intracranial atypical meningioma and to analyze the reasons of misdiagnosis. Methods: Clinical data and MRI features of 9 patients with intracranial atypical meningioma proved by pathology were retrospectively analyzed. Results: Of 9 cases, 4 cases located in brain convexity, 2 cases located in parasagittal and parafalcine, 3 cases located in transverse sinus, cerebellopontine angle region, pontobulbar region, respectively. Before operations, 4 cases were misdiagnosed for gliomas, 2 cases for neurogenic tumors, 2 cases for metastatic tumors, 1 case for cavernous hemangioma. Conclusion: MRI appearances of intracranial atypical meningioma are variable and easy to be misdiagnosed. On the basis of observation of the tumors basic signal characteristics, further analysis of the relationship between tumors and their adjacent tissues, sites of tumors, MRI signal features of rare pathological subtypes of meningioma can contribute to improve the diagnosis accuracy of atypical meningioma. Copyright © 2015 by the Press of Chinese Journal of Medical Imaging and Technology.


PubMed | Shengli Oilfield Central Hospital, Shandong University of Traditional Chinese Medicine, Shandong Academy of Sciences and Shandong University
Type: Journal Article | Journal: American journal of reproductive immunology (New York, N.Y. : 1989) | Year: 2016

Estradiol (E2 ) deficiency can cause bone loss and the skew of Th1/Th2 cells. However, the correlation between the Th1/Th2 cells and the bone loss induced by estrogen deficiency remains unclear. Our aim was to investigate the role of Th1/Th2 in bone loss induced by estrogen deficiency and elucidated the therapeutical effect of catalpol in this condition.Young, sham-operated (Sham), ovariectomized (Ovx), and naturally aged mice, treated with catalpol at different doses or control vehicle, were used in this study as indicated in each experiment. ELISA assay, dual-energy X-ray absorptiometry, and flow cytometry were used to analyze E2 , C-terminal telopeptides of type I collagen (CTx-I), bone mineral density (BMD), and Th1/Th2 subsets, respectively. The mRNA and protein expressions of specific transcription factors for Th1/Th2 cells (T-bet and GATA-3) were analyzed using real-time quantitative PCR and Western blot, respectively.Bone mineral density and E2 levels positively correlated with the proportion of Th2 subset while negatively correlated with that of Th1 subset and the ratio of Th1/Th2. Catalpol alleviated bone loss effectively by regulating Th1/Th2 polarization. Catalpol promoted the expression of Th2-specific transcription factors while inhibited that associated with Th1.Th1/Th2 skew is involved in bone loss induced by estrogen deficiency. Catalpol alleviates bone loss effectively by regulating Th1/Th2 paradigm.


Liu J.,PLA Fourth Military Medical University | Yao Y.,Baoji Central Hospital | Ding H.,Shengli Oilfield Central Hospital | Chen R.,PLA Fourth Military Medical University
Tumor Biology | Year: 2014

With the objective of identifying promising antitumor agents for human leukemia, we carried out to determine the anticancer ability of oxymatrine on the human leukemia HL-60 cell line. In vitro experiments demonstrated that oxymatrine reduced the proliferation of HL-60 cells in a dose- and time-dependent manner via the induction of apoptosis and cell cycle arrest at G2/M and S phases. The proteins involved in oxymatrine-induced apoptosis in HL-60 cells were also examined using Western blot. The increase in apoptosis upon treatment with oxymatrine was correlated with downregulation of anti-apoptotic Bcl-2 expression and upregulation of pro-apoptotic Bax expression. Furthermore, oxymatrine induced the activation of caspase-3 and caspase-9 and the cleavage of poly(ADP-ribose) polymerase (PARP) in HL-60 cells. In addition, pretreatment with a specific caspase-3 (Z-DEVD-FMK) or caspase-9 (Z-LEHD-FMK) inhibitor significantly neutralized the pro-apoptotic activity of oxymatrine in HL-60 cells, demonstrating the important role of caspase-3 and caspase-9 in this process. Taken together, these results indicated that oxymatrine-induced apoptosis may occur through the activation of the caspase-9/caspase-3-mediated intrinsic pathway. Therefore, oxymatrine may be a potential candidate for the treatment of human leukemia. © 2014 International Society of Oncology and BioMarkers (ISOBM).


PubMed | Xijing Hospital, Traditional Chinese Medicine Hospital of Baoji City, PLA Fourth Military Medical University and Shengli Oilfield Central Hospital
Type: | Journal: The American journal of Chinese medicine | Year: 2017

Punicalagin (PUN), a major bioactive component in pomegranate juice, has been proven to exert neuroprotective effects against cerebral ischemia/reperfusion (I/R) insult via anti-oxidant properties. This study aims to investigate whether PUN provides cardioprotection against myocardial I/R (MI/R) injury and the underlying mechanisms. PUN (30[Formula: see text]mg/kg/d) or vehicle was intragastrically administered to Sprague-Dawley rats for one week before the operation. MI/R was induced by ligating the left anterior descending coronary artery for 30[Formula: see text]min and subsequent reperfusion for 3[Formula: see text]h. PUN pretreatment conferred cardioprotective effects against MI/R injury by improving cardiac function, limiting infarct size, reducing serum creatine kinase-MB and lactate dehydrogenase activities, and suppressing cardiomyocyte apoptosis. Moreover, PUN pretreatment inhibited I/R-induced myocardial oxidative stress as evidenced by decreased generation of superoxide content and malonaldialdehyde formation and increased antioxidant capability. Furthermore, PUN pretreatment increased adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) phosphorylation in I/R hearts. AMPK inhibitor compound c inhibited PUN-enhanced AMPK phosphorylation, and blunted PUN-mediated anti-oxidative effects and cardioprotection. These results indicate for the first time that PUN pretreatment protect against I/R-induced oxidative stress and myocardial injury via activation of AMPK.


Xunyi Y.,Qingdao University | Zhentao Y.,Shengli Oilfield Central Hospital | Dandan J.,Qingdao University | Funian L.,Qingdao University
Brazilian Journal of Medical and Biological Research | Year: 2012

Protein tyrosine phosphatase non-receptor type 12 (PTPN12) is a recently identified tumor suppressor gene (TSG) that is frequently compromised in human triple-negative breast cancer. In the present study, we investigated the expression of PTPN12 protein by patients with breast cancer in a Chinese population and the relationship between PTPN12 expression levels and patient clinicopathological features and prognosis. Additionally, we explored the underlying down-regulation mechanism from the perspective of an epigenetic alteration. We examined PTPN12 mRNA expression in five breast cancer cell lines using semi quantitative reverse-transcription PCR, and detected PTPN12 protein expression using immunohistochemistry in 150 primary invasive breast cancer cases and paired adjacent non-tumor tissues. Methylation-specific PCR was performed to analyze the promoter CpG island methylation status of PTPN12. PTPN12 was significantly down-regulated in breast cancer cases (48/150) compared to adjacent noncancerous tissues (17/150; P < 0.05). Furthermore, low expression of PTPN12 showed a significant positive correlation with tumor size (P = 0.047), lymph node metastasis (P = 0.001), distant metastasis (P = 0.009), histological grade (P = 0.012), and survival time (P = 0.019). Additionally, promoter CpG island hypermethylation occurs more frequently in breast cancer cases and breast cancer cell lines with low PTPN12 expression. Our findings suggest that PTPN12 is potentially a methylation-silenced TSG for breast cancer that may play an important role in breast carcinogenesis and could potentially serve as an independent prognostic factor for invasive breast cancer patients.


Sun X.,Nanjing Medical University | Liu W.-J.,Shengli Oilfield Central Hospital | Xu L.-L.,Nanjing Medical University | Ding Q.,Nanjing Medical University | And 4 more authors.
European Spine Journal | Year: 2013

Purpose: Brace treatment has served as a vital non-surgical procedure for immature adolescent idiopathic scoliosis (AIS) patients with a mild or moderate curve. For the patients who fail in bracing and resort to surgery, it is unclear whether prior full-time brace treatment significantly influences outcomes. This study aims to investigate whether prior brace treatment has a negative impact upon the flexibility and correctability of the main curve in patients with AIS. Methods: The participants were collected from female AIS patients who underwent posterior correction surgery with pedicle screw instrumentation from August 2006 to December 2010, with or without prior brace treatment. Patients included in Group A had prior brace treatment over a 1-year period, and underwent surgery within 6 months after cessation of bracing; those in Group B received no prior treatment and were randomly selected from our database. Curve flexibility pre-surgery and curve correctability post-surgery were computed and compared between both groups and subgroups according to the curve location. Results: Each group consisted of 35 patients. Age, curve magnitude and location were comparable between the two groups. Before surgery, patients in Group A had a slightly lower curve flexibility than those in Group B (52 vs. 60 %, P = 0.036). After surgery, satisfactory correction results were observed in both groups, but the average post-operative main curve magnitude of patients in Group B was 4 less than that of Group A (10 vs. 14, P = 0.010). The curve correctability in Group B was significantly higher than that in Group A (80 vs. 74 %, P = 0.002). No matter what curve pattern the patient had, having a prior history of brace treatment resulted in a trend of lower flexibility and correctability of their scoliosis. Conclusions: Good surgical correction can be achieved in AIS patients who have been unsuccessful with prior brace treatment. However, a history of prior brace treatment leads to a trend of lowering the curve flexibility, and in turn, negatively impacts upon the curve correctability. © 2012 Springer-Verlag.


Li S.,Shengli Oilfield Central Hospital | Cui Z.,Shengli Oilfield Central Hospital | Meng X.,Shengli Oilfield Central Hospital
Oncology Research | Year: 2016

Poly(ADP-ribose) polymerase 1 (PARP-1) is reported to be involved in DNA repair and is now recognized as a key regulator in carcinogenesis. However, the potential role and the molecular mechanism underlying the effect of PARP-1 on osteosarcoma (OS) cells have not been elucidated. In this study, the results showed that knockdown of PARP-1 resulted in decreased cell proliferation, increased cell apoptosis, and G0/G1 phase arrest in U2OS cells. In addition, increased expression of active caspase 3 and Bax, but reduced Bcl-2, cyclin D1, and phosphorylated extracellular signal regulated kinase 1/2 (pERK1/2) were observed in PARP-1 knockdown in U2OS cells. Moreover, knockdown of PARP-1 correlated with elevated chemosensitivity of U2OS cells to cisplatin through inactivation of the ERK1/2 signaling pathway. In conclusion, our findings demonstrated that PARP-1 plays an important role in regulating OS growth, combining PARP-1 gene therapy with traditional chemotherapy, and may serve as a promising approach to OS therapy. Copyright © 2016 Cognizant, LLC. All rights reserved.

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