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Li F.-H.,Sun Yat Sen University | Li F.-H.,Shengli Oil Field Central Hospital | Shen L.,Peking University | Li Z.-H.,Sun Yat Sen University | And 5 more authors.
World Journal of Gastroenterology | Year: 2010

Aim: To investigate the prognostic value of KRAS mutation, and phosphatase and tensin (PTEN) expression in Chinese metastatic colorectal cancer metastatic colorectal cancer (mCRC) patients treated with cetuximab. Methods: Ninety Chinese mCRC patients treated with cetuximab were evaluated for KRAS mutation and PTEN protein expression by DNA sequencing of codons 12 and 13 and immunohistochemistry, respectively. We then selected 61 patients treated with cetuximab, either in combination with chemotherapy, or alone as a second-line or third-line regimen to assess whether KRAS mutation or PTEN protein expression is associated with the response and the survival time of mCRC patients treated with cetuximab. Results: KRAS mutation was found in 30 (33.3%) tumor samples from the 90 patients, and positive PTEN expression was detected in 58 (64.4%) of the 90 patients. Among the 61 patients who were treated with cetuximab as a second-line or third-line regimen, the resistance to cetuximab was found in 22 patients with KRAS mutation and in 39 patients without KRAS mutation, with a response rate of 4.5% and 46.1% respectively (P = 0.001), a shorter median progression-free survival (PFS) time of 14 ± 1.3 wk and 32 ± 2.5 wk respectively (P < 0.001), a median overall survival (OS) time of 11 ± 1.2 mo and 19 ± 1.8 mo respectively (P < 0.001), as well as in 24 patients with negative PTEN expression and in 37 patients with positive PTEN expression respectively (P < 0.001), with a responsive rate of 4.2% and 48.6% respectively, a shorter median PFS survival time of 17 ± 2.0 wk and 28 ± 1.9 wk respectively (P = 0.07), and a median OS time of 11 ± 1.3 mo and 18 ± 1.9 mo respectively (P = 0.004). Combined KRAS mutation and PTEN expression analysis showed that the PFS and OS time of patients with two favorable prognostic factors were longer than those of patients with one favorable prognostic factor or no favorable prognostic factor (P < 0.001). Conclusion: KRAS mutation and PTEN protein expression are significantly correlated with the response rate and survival time of Chinese mCRC patients treated with cetuximab. © 2010 Baishideng.


Cui L.-H.,Qingdao University | Quan Z.-Y.,Yanbian University | Piao J.-M.,Qingdao University | Zhang T.-T.,Qingdao University | And 4 more authors.
International Journal of Molecular Sciences | Year: 2016

Folate and vitamin B12 involved in the one-carbon metabolism may play a key role in carcinogenesis and progression of hepatocellular carcinoma (HCC) through influencing DNA integrity. The purpose of this study is to evaluate the association of plasma folate and vitamin B12 levels with HCC in a case-control study on 312 HCC patients and 325 cancer-free controls. Plasma concentrations of folate and vitamin B12 in all the subjects were measured by electrochemiluminescence immunoassay. Meanwhile, the information of HCC patients’ clinical characteristics including tumor-node-metastasis (TNM) stage, tumor size and tumor markers were collected. The patients of HCC had significantly lower folate levels than those of controls; there was no significant difference in the mean of plasma vitamin B12 levels. We also observed an inverse association between the levels of plasma folate and HCC: the adjusted odds ratios (OR) (95% confidence intervals (CI)) of HCC from the highest to lowest quartile of folate were 0.30 (0.15-0.60), 0.33 (0.17-0.65), and 0.19 (0.09-0.38). Compared to the subjects in the lowest quartile of plasma vitamin B12, only the subjects in the highest quartile of vitamin B12 exhibited a significant positive relationship with HCC, the adjusted OR was 2.01 (95% CI, 1.02-3.98). HCC patients with Stage III and IV or bigger tumor size had lower folate and higher vitamin B12 levels. There was no significant difference in the mean plasma folate levels of the HCC cases in tumor markers status (AFP, CEA and CA19-9 levels), whereas patients with higher CEA or CA19-9 levels retained significantly more plasma vitamin B12 than those with normal-CEA or CA19-9 level. In conclusion, plasma folate and vitamin B12 levels could be associated with HCC, and might be used as predictors of clinical characteristics of HCC patients. However, further prospective studies are essential to confirm the observed results. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Tianjin Entry Exit Inspection and Quarantine Bureau, U.S. Center for Disease Control and Prevention, Shandong University and Shengli Oil Field Central Hospital
Type: Journal Article | Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2016

To investigate the association between serum direct bilirubin (DBIL) with metabolic syndrome (MS) and its components.A dynamic health check-up cohort study was set up from 2006 to 2011. 5 258 participants who satisfied the two basic rules: 1) being free of MS at the 1(st) health check-up program; 2) having at least two intact health checks were included in this study. With generalized estimating equation (GEE) model, after adjusting for items as age, gender, smoking, drinking, alanine aminotransferase, aspartate aminotransferase, -glutamyltransferase, uric acid, blood urea nitrogen and white blood cells, the multivariable relative risks (RRs) of DBIL with MS and their components were analyzed.The RRs of DBIL for MS was 0.722 (95%CI: 0.654-0.797), which showing a dose-response. Serum DBIL was negatively associated with obesity and hyperlipidemia. Taking factors as gender and age into account, in the <45 years and 45-55 years groups, the RRs of DBIL for MS in females appeared as 0.516 (95%CI: 0.349-0.761) and 0.435 (95%CI: 0.256-0.740). And in males of <45 years and 45-55 years, the RRs of DBIL for MS were 0.738 (95% CI: 0.644-0.846) and 0.790 (95% CI:0.667-0.937), respectively.The elevated DBIL levels in serum appeared an early biomarker for MS and its components (obesity and hyperlipidemia). In particular,they may play a role in reducing the risk of MS in both females and males before 55 years of age.


Ding H.-F.,Shengli Oil Field Central Hospital | Zhang H.,Qingdao Municipal Hospital | Ding H.-F.,Shandong University | Li D.,Shandong University | And 4 more authors.
World Journal of Pediatrics | Year: 2014

Background: Oxidative stress is involved in the development of hypoxic-ischemic brain damage (HIBD). In this study, we investigated the therapeutic effects of placenta-derived mesenchymal stem cells (PD-MSCs) and explored the NF-E2-related factor-2/heme oxygenase-1 (Nrf2/HO-1) signaling pathway in treating HIBD.Methods: P7 rats were subjected to hypoxic-ischemic brain injury and randomly divided into four groups (control, HIBD, HIBD+PD-MSCs, and HIBD+fibroblasts). Forty-eight hours after the induction of HIBD, 5×105 of PD-MSCs were injected into cerebral tissue in the HIBD+PD-MSCs group, while the same dose of fibroblasts were injected in the HIBD+fibroblasts group. Morris Water Maze, gross and pathological changes were tested at P28. The level of malondialdehyde (MDA) was detected in rats’ hippocampus. RT-PCR and western blot analysis were used to evaluate the changes of Nrf2/HO-1.Results: The HIBD group showed significantly longer escape latency and a lower frequency of original platform crossing in the Morris Water Maze compared with the control group. Rats receiving PD-MSCs showed significant improvement of HIBD. The pathological changes were evident after HIBD, but ameliorated in the PD-MSCs group. Compared with the control group, HO-1 and Nrf2 were up-regulated at gene and protein levels in the HI brain, beginning at 6 hours and peaking at 48 hours (P<0.05). The expression of HO-1 and Nrf2 in the PD-MSCs treatment group was more pronounced than in the HIBD group (P<0.01). PD-MSCs also decreased MDA production in the brain tissue.Conclusion: These results demonstrate that PD-MSCs have neuroprotective effect during the treatment of HIBD and that the mechanism may be partly due to alleviating oxidative stress. © 2014, Children's Hospital, Zhejiang University School of Medicine and Springer-Verlag Berlin Heidelberg.


Han X.,Tianjin Medical University | Han X.,Shengli Oil Field Central Hospital | Jiang K.,Tianjin Medical University | Wang B.,Tianjin Medical University | And 3 more authors.
Clinical Drug Investigation | Year: 2015

Background and Objective: Chronic gastritis frequently progresses into precancerous intestinal metaplasia and intraepithelial neoplasia lesions. Rebamipide is a free radical scavenger and we assessed its efficacy on clinical symptoms, gastric mucosal lesions, pathologic grade, and immunohistochemistry in chronic gastritis patients. Methods: 178 eligible patients were randomized into treatment and control groups. Both groups followed an optimized lifestyle for 26 weeks, but the treatment group was additionally medicated with rebamipide 0.1 g three times per day. Upper gastrointestinal endoscopy was performed in all patients to evaluate the severity of gastritis by the Modified Lanza Scoring (MLS) and histological changes were evaluated by the Updated Sydney System Score (USSS). Gastric mucosa immunohistochemistry in the treatment group was performed using the intestinal metaplasia markers caudal type homeobox transcription factor 2 (CDX2) and trefoil factor 3 (TFF3) detection. Results: There were significant outcome differences between the treatment and control groups regarding the clinical symptom scores (2.62 ± 1.86 vs. 1.55 ± 1.61, P = 0.0001), gastric mucosal lesion scores (0.57 ± 1.05 vs. 0.16 ± 0.90, P = 0.002), and inflammation (P < 0.05). Only in the treated patients were the rates of intestinal metaplasia (P = 0.017 vs. P = 0.123) and low-grade intraepithelial neoplasia (P = 0.005 vs. P = 0.226) significantly reduced after 26 weeks. The percentages of CDX2 (31.5 vs. 15.7 %, P = 0.021) and TFF3 (44.9 vs. 25.8 %, P = 0.012) expressing gastric mucosa cells were significantly lower after rebamipide medication than pre-treatment values. Conclusions: Rebamipide improved the clinical symptoms, gastric mucosal lesions, and pathological grades of chronic gastritis patients and decreased the expression rates of CDX2 and TFF3 in gastric cells. © 2015, Springer International Publishing Switzerland.


Yu Y.-Q.,Shengli Oil Field Central Hospital | Liu H.-Q.,Shengli Oil Field Central Hospital | Chen T.-S.,Shengli Oil Field Central Hospital | Zhou F.,Shengli Oil Field Central Hospital | Lv Q.-J.,Shengli Oil Field Central Hospital
World Chinese Journal of Digestology | Year: 2013

Aim: To observe the clinical efficacy of immunoglobulin (IG) in the treatment of infection in patients with cirrhosis. Methods: One hundred and five cirrhotic patients with an infection were randomized into either a control group or an observation group. The control group received routine antibiotic treatment, while the observation group was given IG on the basis of routine antibiotic treatment and underwent efficacy evaluation using Child-Pugh scores. Serum total bilirubin (TB), albumin, alanine aminotransferase (ALT), total cholesterol (TC) and blood coagulation parameters were monitored. The rate of early infection control and incidence of complications were compared between the two groups. Results: For patients with a Child-Pugh score < 10, the rate of early infection control was not statistically significant between the two groups (P > 0.05). For patients with a Child-Pugh score between 10 and 15, the rate of infection control was significantly higher in the observation group than in the control group (84.00% vs 46.15%, P < 0.05). Severe infection occurred during therapy in two cases in the observation group and in eight cases in the control group, and there was no significant difference in the number of cases of severe infection occurring during therapy between the two groups (P > 0.05). In addition, 11 cases in the observation group and 13 cases in the control group were actively discharged from the hospital or died, and there was no statistically significant difference in the number of patients who were actively discharged from the hospital or died between the two groups (P > 0.05).Conclusion: IG can effectively improve the rate of infection control and reduce the incidence of complications during hospitalization and mortality in cirrhotic patients with a Child-Pugh score between 10 and 15. © 2013 Baishideng. All rights reserved.


Cheng B.,Shengli Oil Field Central Hospital | Cai Q.,Tianjin Medical University | Wu Y.,Tianjin Medical University | Zhao Y.,Tianjin Medical University | And 5 more authors.
Oncology Letters | Year: 2015

The aim of the present study was to investigate the clinical characteristics and management of primary renal sinus tumors. We retrospectively analyzed three cases of primary renal sinus tumors. The first patient was a 33-year-old man who presented with right flank pain for 6 months. Based on the imaging results, the patient was diagnosed with renal sinus tumor. The second case was a 34-year-old woman who presented with sudden lumbago in the right flank for 3 days. The imaging results confirmed the diagnosis of right renal angiomyolipoma. The third case was a 55-year-old woman with flank pain, which had persisted for 1 year. The imaging tests revealed lipoma of the left renal sinus. All three cases underwent surgical procedures. The first case was diagnosed with benign angioleiomyoma following pathological analysis. During surgery, the tumor was ablated and the kidney was spared. The second case was scheduled for tumor enucleating, but a nephrectomy was performed due to serious hemorrhaging and a damaged renal pelvis. Pathological analysis identified angiomyolipoma. The third case was scheduled for lipoma enucleating; however, nephrectomy was performed as the tumor encapsulated the renal vascular pedicle. Pathological analysis revealed lipoma. In the three cases, no relapse over 3 years, 10 months and 4 years of follow-up, respectively, was observed. In addition, this review examined previous literature and concluded that the occurrence of tumors in the renal sinus is rare and the majority of such tumors are benign. Furthermore, cases are easily misdiagnosed as renal pelvic tumors. Computed tomography, magnetic resonance imaging and intravenous urography are the best imaging examination methods for differential diagnosis. In conclusion, surgery is the usual approach for the treatment of renal sinus tumors and radical nephrectomy should be performed for malignant tumors. © 2015, Spandidos Publications. All rights reserved.


Wang Q.-Q.,Shengli Oil Field Central Hospital | Zhai Y.-J.,Shengli Oil Field Central Hospital | Zhang J.,Shengli Oil Field Central Hospital | Jia X.-G.,Shengli Oil Field Central Hospital | Wang J.-E.,Shengli Oil Field Central Hospital
International Journal of Ophthalmology | Year: 2011

AIM: To assess and compare the thicknesses of central fovea of macula and retinal nerve fiber layer in normal children and children with ametropic amblyopia. METHODS: Optical Coherence Tomography (OCT) was performed on 20 children (40 eyes) with ametropic amblyopia, the mean age was 6.15 ± 1.64 years old, spherical equivalent was 1.50-7.00D, axis oculi was 22.07 ± 0.97mm; OCT was also performed on 25 normal children (50 eyes), the mean age was 8.62 ± 2.42 years old, spherical equivalent was 0.57 ± 1.07D, axis oculi was 22.81 ± 0.72mm. Thicknesses of central fovea of macula and retinal nerve fiber layer were measured. In this study, t-test and Pearson test with SPSS 13.0 software were used to assess and compare the thicknesses of central fovea of macula and retinal nerve fiber layer in normal children and children with ametropic amblyopia. RESULTS: The average thickness of the fovea was 136.60 ±13.82 μm in normal eyes and 132.98 ±14.99 μm in amblyopic eyes. The difference between the two groups was not statistically significant (P > 0.05). The average thicknesses of the retinal nerve fiber layer was 110.40 ± 7.63 μm in normal eyes and 116.95 ±9.59 μm in amblyopic eyes. The difference between the two groups was statistically significant (P < 0.01). There was negative correlation between average thicknesses of the retinal nerve fiber layer and axis in normal eyes (r = -0.31, P < 0.05), but there was no significantly correlation in amblyopic eyes(r=0.12, P >0.05). CONCLUSION: Retinal nerve fiber layer thickness may be affected by ametropic amblyopia, but further histopathologic confirmation is needed.


PubMed | Qingdao University, Shandong University and Shengli Oil field Central Hospital
Type: | Journal: Cellular & molecular immunology | Year: 2015

Hypoxic-ischemic brain damage (HIBD) is a common cause of infant death. The purpose of our research was to explore the immunoregulatory mechanism of placenta-derived mesenchymal stem cells (PD-MSCs) in HIBD treatment. Seven-day-old rat pups were randomly divided into HIBD, PD-MSC, fibroblast, and control groups. Forty-eight hours after HIBD induction, cells at a density of 5 104 cells/10 l were injected into the cerebral tissue in the PD-MSC and fibroblast groups. The TNF-, interleukin- 17 (IL-17), interferon- (IFN-), and IL-10 levels were detected through quantitative real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Regulatory T cell (Tregs) populations were detected through flow cytometry, and forkhead box P3 (Foxp3) was measured through western blot analysis. Behavioral tests and gross and pathological examinations showed that PD-MSC treatment exerted significantly stronger neuroprotective effects than the other treatments. The expression levels of pro-inflammatory cytokines were substantially upregulated after HI injury. Compared with fibroblast treatment, PD-MSC treatment inhibited the production of pro-inflammatory cytokines and increased the production of IL-10 in the ischemic hemispheres and peripheral blood serum (all P < 0.01). Flow cytometry results showed a notable increase in the number of Tregs within the spleen of the HIBD group. Moreover, the number of Tregs and the Foxp3 expression levels were higher in the PD-MSC treatment group than in the HIBD and fibroblast groups (all P < 0.01). Our research suggests that the mechanism of PD-MSC treatment for HIBD partially involves inflammatory response suppression.Cellular & Molecular Immunology advance online publication, 28 December 2015; doi:10.1038/cmi.2015.99.


PubMed | Chinese National Institute for Viral Disease Control and Prevention and Shengli Oil Field Central Hospital
Type: Journal Article | Journal: Archives of virology | Year: 2016

Enterovirus 71 (EV71) is one of the major causative agents of outbreaks of hand, foot, and mouth disease (HFMD). A commercial TaqMan probe-based real-time PCR assay has been widely used for the differential detection of EV71 despite its relatively high cost and failure to detect samples with a low viral load (Ct value>35). In this study, a highly sensitive real-time nested RT-PCR (RTN RT-PCR) assay in a single closed tube for detection of EV71 in HFMD was developed. The sensitivity and specificity of this assay were evaluated using a reference EV71 stock and a panel of controls consisting of coxsackievirus A16 (CVA16) and common respiratory viruses, respectively. The clinical performance of this assay was evaluated and compared with those of a commercial TaqMan probe-based real-time PCR (qRT-PCR) assay and a traditional two-step nested RT-PCR assay. The limit of detection for the RTN RT-PCR assay was 0.01TCID50/ml, with a Ct value of 38.3, which was the same as that of the traditional two-step nested RT-PCR assay and approximately tenfold lower than that of the qRT-PCR assay. When testing the reference strain EV71, this assay showed favorable detection reproducibility and no obvious cross-reactivity. The testing results of 100 clinical throat swabs from HFMD-suspected patients revealed that 41 samples were positive for EV71 by both RTN RT-PCR and traditional two-step nested RT-PCR assays, whereas only 29 were EV71 positive by qRT-PCR assay.

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