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Zhou Y.,Shengjing Hospital of China Medical UniversityLiaoning | Chen Y.-Y.,Shengjing Hospital of China Medical UniversityLiaoning | Zhang X.-Y.,Shengjing Hospital of China Medical UniversityLiaoning | Tan M.-Q.,Shengjing Hospital of China Medical UniversityLiaoning | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

Objective: NF-κB, especially p65 subunit, plays important role in the process of pulmonary fibrosis. In this study, we transformed fibroblast into myofibroblast induced by bleomycin, and then studied the effects of NF-κB p65 antisense oligonucleotide on pulmonary fibrosis in mouse model. Methods: Pulmonary fibrosis was induced by bleomycin in C57BL/6 mouse (modeling group). The NF-κB antisense oligonucleotide was injected intravenously into mouse 6 hours before inducing (test group), we performed broncho-alveolar lavage and blood collecting through cardiac puncture. Bronchoalveolar Lavage Fluid (BALF) and serum from normal C57BL/6 mouse (control group) were collected for comparison. Immunohistochemistry staining of the NF-κB and α-SMA on lung tissues and cultured cells were carried out in each group, respectively. Results: The expression level of NF-κB and α-SMA were both consistently higher in modeling group when compared with control group (P < 0.05). Meanwhile, they were reduced significantly through the intervention of NF-κB p65 antisense oligonucleotide in the test group (P < 0.05). More importantly, the expression of NF-κB was positively correlated with α-SMA. Conclusion: our study suggests the potential in prevention of bleomycin-induced pulmonary fibrosis with NF-κB p65 antisense oligonucleotide. © 2014, E-Century Publishing Corporation. All Rights Reserved. Source

Han J.,Shengjing Hospital of China Medical UniversityLiaoning | Xia J.,Shengjing Hospital of China Medical UniversityLiaoning | He Q.,Shengjing Hospital of China Medical UniversityLiaoning | Shao Y.,Shengjing Hospital of China Medical UniversityLiaoning | And 3 more authors.
Singapore Medical Journal | Year: 2016

INTRODUCTION An accurate assessment of peer victimisation (i.e. bullying) is a necessary precondition for research and intervention. Most assessment instruments use the ‘list of acts’ measurement strategy, which does not account for the actual physical and psychological damage inflicted by bullying. To resolve this limitation, this study developed a peer victimisation scale (PVS) that includes harmful consequences for judgement and measurement of peer victimisation. METHODS The PVS is a 40-item self-report questionnaire designed to assess the four aspects of peer victimisation: physical, verbal, relational, and interference and control. A total of 1,469 Grade 3–8 students (49.9% male) were recruited to test the psychometric properties of the PVS. Another 420 Grade 3–8 students were examined by a modified PVS supplemented with a semi-structured interview for scale validation and establishment of the cut-off points for severe bullying. Incidence, age and gender distribution of peer victimisation were also analysed. RESULTS The PVS demonstrated good internal consistency reliability (Cronbach’s alpha 0.73–0.83) and test-retest reliability two weeks later (correlation coefficient [r] = 0.71–0.80). The scores for each dimension were significantly and positively correlated with the scores from the questionnaire-interview sample (r = 0.73–0.78), and modestly correlated with the scores for symptoms of anxiety and depression (r = 0.36–0.54). CONCLUSION The results were consistent with the measurement constructs, demonstrating that the PVS is a reliable and effective instrument for assessing peer victimisation in children. It may enable more reliable longitudinal studies assessing the impact of peer victimisation to be conducted. © 2016, Singapore Medical Association. All Rights Reserved. Source

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