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Wang L.,Liaoning Medical University | Wang J.,Liaoning Medical University | Jin Y.,Liaoning Medical University | Gao H.,Shengjing Hospital of China Medical University | Lin X.,Liaoning Medical University
Journal of Periodontology | Year: 2014

A T-helper 17 (Th17)/regulatory T (Treg) imbalance has been suggested recently to play a role in the development of periodontitis. All-trans retinoic acid (ATRA) has been reported to modulate Th17/Treg imbalances in some diseases. However, the effect of ATRA on periodontitis remains unknown. This study observes the effect of ATRA on Th17/Treg imbalance modulation in experimental periodontitis. Methods: Experimental periodontitis was induced in mice by oral infection with Porphyromonas gingivalis (P. gingivalis). ATRA was orally administered every other day. Alveolar bone resorption (ABR) was estimated by measuring the distance from the cemento-enamel junction to the alveolar bone crest. CD4+ T-cell subsets in the cervical lymph nodes (CLNs) and spleen were analyzed by flow cytometry. Th17/Treg cell-related cytokine messenger ribonucleic acid expression was quantified by real-time reverse transcription-polymerase chain reaction. Results: The present data shows that ATRA suppressed ABR and inhibited inflammatory cell infiltration into periodontal tissues. These effects were closely associated with reduced CD4 + retinoid-related orphan receptor γτ+ cells and increased CD4+ forkhead box P3+ cells in the CLNs. Furthermore, ATRA downregulated interleukin (IL)-17A expression and upregulated IL-10 and transforming growth factor-β1 expression in both the CLNs and P. gingivalis-infected gingival tissues. Conclusions: These results suggest that ATRA modulation of the Th17/Treg imbalance provides protection against periodontitis by enhancing Treg cell activation and inhibiting Th17 cell activation. These results indicate the potential for clinical prevention of periodontitis.


Zhou X.-M.,Shengjing Hospital of China Medical University | Li P.,Shengjing Hospital of China Medical University | Zhao L.,Shengjing Hospital of China Medical University | Shi W.-J.,Shengjing Hospital of China Medical University
Internal Medicine | Year: 2015

Tracheobronchial aspergillosis is a form of invasive aspergillosis limited to the airways. Its presence usually indicates an immunocompromised status or local dysfunction of airway immunity. We herein report a case of lung carcinosarcoma masked by tracheobronchial aspergillosis and discuss the clinical manifestations, diagnostic procedures and treatment of such cases. Tracheobronchial aspergillosis may also mask endobronchial carcinoma, leading to difficulties in determining the diagnosis and selecting subsequent treatment. Bronchoscopy is crucial for obtaining a definitive diagnosis and confirming the treatment. In particular, the combination of intravenous antifungal drug administration, localized antifungal application and interventional bronchoscopy is effective. Nevertheless, clinicians should always investigate the underlying disease carefully, including the potential occurrence of lung malignancy. © 2015 The Japanese Society of Internal Medicine.


Li B.,Shengjing Hospital of China Medical University | Ling O.,Shengjing Hospital of China Medical University | Han X.,Shengjing Hospital of China Medical University | Zhou Y.,Shengjing Hospital of China Medical University | And 3 more authors.
OncoTargets and Therapy | Year: 2013

Primary primitive neuroectodermal tumors (PNETs) are rare and high-grade malignant tumors that mostly occur in children and young adults. The most common sites are the trunk, limbs, and retroperitoneum. Herein, we present a case of a PNET involving the cervix uteri in a 27-year-old woman. The lesion showed characteristic histologic features of a PNET and was positive for the immunohistochemical markers cluster of differentiation (CD) 99, vimentin, neuron-specific enolase, neural cell adhesion molecule 1 (CD56), and CD117 (c-kit), further defining the tumor while helping to confirm PNET. The clinical Stage IIIB tumor was treated with chemotherapy and radiotherapy. © 2013 Li et al, publisher and licensee Dove Medical Press Ltd.


Yu D.,Shengjing Hospital of China Medical University | Tang S.,Shengjing Hospital of China Medical University
Internal Medicine | Year: 2013

A perivascular epithelioid cell tumor (PEComa) is a rare type of mesenchymal tumor. Cases that arise from the liver are extremely rare. We report a case of a 41-years-old woman suffering from a hepatic PEComa with an emphasis on its imaging findings, primarily those of contrast-enhanced computer tomography (CECT) and sonography. We also conducted a literature review to evaluate imaging findings that could provide some information for preoperative diagnosis. © 2013 The Japanese Society of Internal Medicine.


Qiao L.,Shengjing Hospital of China Medical University | Feng Y.,Shengjing Hospital of China Medical University
Gene | Year: 2012

The associations between polymorphisms of prostate stem cell antigen (PSCA-rs2294008C>T and -rs2976392G>A) and gastric cancer (GC) risk for Eastern Asians have been commonly studied, but the results were conflicting. The aim of the present study was to further assess the associations by the method of meta-analysis. The databases of Medline, Embase and CNKI (up to May 25th, 2011) were retrieved to identify eligible case-control studies. Odds ratio (OR) and 95% confidence interval (95%CI) were used to present the strength of the associations. In total, eight case-control studies in seven articles with 16792 individuals (9738 cases of GC and 7054 controls) were included in this meta-analysis. Through quantitative analyses, we found that T allele of rs2294008C>T and A allele of rs2976392G>A were significantly associated with increased GC risk [rs2294008C>T: OR (95%CI)=1.31 (1.22-1.42), P z-test<0.001, P heterogeneity=0.166 for TT vs. C carriers; rs2976392G>A: OR (95%CI)=1.36(1.24-1.50), P z-test=0.015, P heterogeneity=0.111 for AA vs. G carriers]. The results of subgroup analyses (according to histopathology, countries and sources of controls) indicated that T allele of rs2294008C>T and A allele rs2976392G>A were associated with increased risk of both intestinal- and diffuse-type GC, and associated with increased risk of GC for Chinese, Japanese, Koreans, PCC and HCC/PHCC. Furthermore, T allele of rs2294008C>T was also associated with increased risk of cardia and non-cardia GC, and associated with increased risk of GC for males and females. Besides those, this meta-analysis also indicated that the interactions between T allele of rs2294008C>T and A allele of rs2976392G>A was associated with increased risk of GC (A-T vs. G-T: OR=1.16, 95%CI=1.06-1.27, P z-test=0.001, P heterogeneity=0.835). Although modest limitations and potential bias cannot be eliminated, this meta-analysis suggests that PSCA -rs2294008C>T and -rs2976392G>A are potential factors of GC development for Eastern Asians, and future work may incorporate these findings and evaluate these variants as potential markers for screening and early diagnosis of GC. © 2011.


Zhang G.-J.,Shengjing Hospital of China Medical University | Zhang Z.,Shenyang University
Asian Pacific Journal of Cancer Prevention | Year: 2013

Resistance to apoptosis is a major obstacle preventing effective therapy for malignancies. Bcl-2 plays a significant role in inhibiting apoptosis. We reconstructed a stable human Bcl-2 transfected cell line, BIU87- Bcl-2, that was derived from the transfection of human bladder carcinoma cell line BIU87 with a plasmid vector containing recombinant Bcl-2 [pcDNA3.1(+)-Bcl-2]. A cell line transfected with the plasmid alone [pcDNA3.1(+)- neo] was also established as a control. BIU87 and BIU87-neo proved sensitive to adriamycin induced apoptosis, while BIU87-Bcl-2 was more resistant. In view of the growing evidence that NF-κB may play an important role in regulating apoptosis, we determined whether Bcl-2 could modulate the activity of NF-κB in bladder carcinoma cells. Stimulation of BIU87, BIU87-neo and BIU87-Bcl-2 with ADR resulted in an increase expression of NF-κB (p<0.001). The expression of NF-κB in BIU87-Bcl-2 was higher than in the other two cases, with a concomitant reduction in the IκBκ protein level. These results suggest that the overexpression of Bcl-2 renders human bladder carcinoma cells resistant to adriamycin -induced cytotoxicity and there is a link between Bcl-2 and the NF-κB signaling pathway in the suppression of apoptosis.


Wei B.,Shengjing Hospital of China Medical University | Zhang H.,Shengjing Hospital of China Medical University | Li L.,Shengjing Hospital of China Medical University | Li M.,Shengjing Hospital of China Medical University | Shang Y.,Shengjing Hospital of China Medical University
Journal of International Medical Research | Year: 2011

Asthma is a chronic inflammatory disorder of the lung, which is thought to be determined by the balance between the T helper (Th)2 and Th1 responses. This study evaluated whether the balance between Th17 cells and regulatory T cells (T reg) was impaired in asthma patients. The proportion of peripheral blood Th17 cells of the total CD4 + cell population in asthma patients was significantly higher than in controls (mean ± SD 0.72 ± 0.5% versus 0.31 ± 0.4%, respectively). The proportion of peripheral Treg cells in asthma patients was significantly lower than in controls (mean ± SD 12.1 ± 4.6% versus 27.2 ± 7.5%, respectively). Analysis of mRNA generally confirmed the flow cytometry data, suggesting that the changes in cytokine levels were mediated at the transcription level. In paediatric asthma patients, the CD4+ T-cell phenotype was skewed toward the Th17 phenotype, suggesting that a Th17/T reg functional imbalance plays a role in asthma. © 2011 Field House Publishing LLP.


Tian Z.,Shengjing Hospital of China Medical University | Li Y.-L.,Shenyang University | Zhao L.,Shengjing Hospital of China Medical University | Zhang C.-L.,Shengjing Hospital of China Medical University
Asian Pacific Journal of Cancer Prevention | Year: 2012

Published data on any association between the CYP2E1 RsaI/PstI (c1/c2) polymorphism and liver cancer risk among east Asians are inconclusive. The aim of this Human Genome Epidemiology (HuGE) review and meta-analysis was to derive a more precise estimation of the relationship. A literature search of Pubmed, Embase, Web of science and CBM databases from inception through July 2012 was conducted. Twelve case-control studies were included with a total of 1,552 liver cancer cases and 1,763 healthy controls. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of association under five genetic models. When all the eligible studies were pooled into the meta-analysis, the results showed that the c2 allele and the c2 carrier (c2/c2 + c2/c1) of RsaI/PstI polymorphism were associated with decreased risk of liver cancer among east Asians (c2 vs. c1: OR = 0.75, 95%CI: 0.59-0.95, P = 0.016; c2/c2 + c2/c1 vs. c1/c1: OR = 0.76, 95%CI: 0.58-1.00, P = 0.050). In the stratified analysis by country, significant associations were observed between RsaI/PstI polymorphism and decreased risk of liver cancer among the Chinese population (c2 vs. c1: OR = 0.70, 95%CI: 0.54-0.91, P = 0.007; c2/c2 + c2/c1 vs. c1/c1: OR = 0.72, 95%CI: 0.54-0.95, P = 0.020), but not among Japanese and Korean populations. Results from the current meta-analysis indicates that the c2 allele of CYP2E1 RsaI/PstI (c1/c2) polymorphism may be a protective factor for HCC among east Asians, especially among China populations.


Jin Z.,Shengjing Hospital of China Medical University
Zhonghua fu chan ke za zhi | Year: 2011

To explore the relationship between sex hormone-binding globulin (SHBG) of gestational diabetes mellitus (GDM) pregnant women with well-controlled glucose and pregnancy outcomes. Two hundred and fifty-one GDM pregnant women of 24 - 28 weeks in Shengjing Hospital of China Medical University were recruited from Mar. 2005 to Mar. 2010. Two hundred and sixteen cases of GDM with well-controlled glucose were defined as glycemic satisfied group, and they were treated by diet therapy (169 cases) or insulin therapy (47 cases). Thirty-five cases with unsatisfied glucose were defined as glycemic unsatisfied group. One hundred and ninety-two healthy pregnant women of 24 - 28 weeks were defined as healthy control group. Serum SHBG and homeostasis model analysis of insulin resistance (HOMA-IR) at 24 - 28 weeks and above 36 weeks were measured. GDM was diagnosed by "two-step" method according to the National Diabetes Data Group (NDDG) criteria. The pregnancy outcomes and complications of the three groups were recorded. (1) Comparison of pregnancy outcomes and complications:glycemic satisfied group was less likely to develop hypertensive disorders in pregnancy (10.6%), premature birth (8.3%), large for gestational age (LGA) (8.8%), neonatal asphyxia (3.7%) and neonatal hypoglycemia (2.3%) compared to glycemic unsatisfied group (42.9%, 34.3%, 31.4%, 22.9% and 11.4%, respectively). And the difference was statistically significant (P < 0.05 or P < 0.01). There was no significant difference for incidence of polyhydramnios, pueperal infection, postpartum hemorrhage, neonatal hyperbilirubinemia between the two groups (P > 0.05). When compared to healthy control group (7.3%, 2.1%, 4.2%, 2.1% and 1.6%), no significant difference was found for incidence of premature birth (8.3%), pueperal infection (3.2%), postpartum hemorrhage (5.1%), neonatal asphyxia (3.7%) and neonatal hypoglycemia (2.3%, P > 0.05). (2) Comparison of results of 24 - 28 weeks and above 36 weeks: serum SHBG of glycemic satisfied group [(384 ± 88), (457 ± 48) nmol/L] was significantly higher than that of glycemic unsatisfied group [(313 ± 45), (401 ± 73) nmol/L]; HOMA-IR of glycemic satisfied group (5.3 ± 1.1, 5.5 ± 1.1) was significantly lower than that of glycemic unsatisfied group (7.0 ± 1.3, 7.6 ± 1.7; P < 0.01). Serum SHBG of glycemic satisfied group was significantly lower than that of healthy control group [(492 ± 95), (565 ± 40) nmol/L]; and HOMA-IR of glycemic satisfied group (5.3 ± 1.1, 5.5 ± 1.1) was significantly higher than that of healthy control group (3.6 ± 0.6, 3.9 ± 0.5; P < 0.01). FPG of glycemic satisfied group [(5.84 ± 0.28), (5.16 ± 0.13) mmol/L] was significantly lower than that of glycemic unsatisfied group [(6.13 ± 0.16), (5.68 ± 1.14) mmol/L;P < 0.01]. FINS of glycemic satisfied group [(20.4 ± 2.1), (24.1 ± 4.2) mmol/L] was significantly lower than that of glycemic unsatisfied group [(24.7 ± 4.5), (29.9 ± 2.7) mmol/L; P < 0.01]. (3) Correlation analysis. Between 24-28 weeks, SHBG was negatively correlated with HOMA-IR in the three groups (r = -0.952, P < 0.01); and SHBG was negatively correlated with HOMA-IR in glycemic satisfied group (r = -0.903, P < 0.01). Well-controlled glucose can not completely improve maternal and fetal outcomes of GDM pregnant women. High insulin resistance and low serum SHBG can influence pregnancy outcomes.


PubMed | Shengjing Hospital of China Medical University and Shenyang University
Type: | Journal: Toxicology letters | Year: 2017

This study investigated the role of myeloid differentiation factor 88 (MyD88) in paraquat-induced acute lung injury (ALI).C57BL mice were divided into the control group, paraquat group, MyD88 knockout (KO) group, and MyD88 KO plus paraquat group. At 48h after paraquat poisoning, serum and lung tissues were collected. ELISA was employed to detect tumor necrosis factor- (TNF-) and interleukine-1 (IL-1) contents in serum. Lung tissues were processed for hematoxylin-eosin staining, followed by histological scoring. PCR was performed to detect the mRNA expression of MyD88, TNF-, and IL-1 in the lungs. Immunofluorescence staining was done to evaluate the expression and distribution of MyD88 and nuclear factor B (NF-B) in the lungs. Western blotting was conducted to detect the protein level of toll-like receptor (TLR) 4, TLR9, MyD88, and NF-B in the lungs.Paraquat poisoning significantly increased serum inflammatory cytokines, as well as MyD88, TLR4, TLR9, and NF-B, and resulted in ALI. After MyD88 KO, the levels of inflammatory cytokines and NF-B decreased markedly, and ALI was also attenuated although TLR4 and TLR9 expression continued at an elevated level.MyD88 mediates paraquat-induced ALI, and MyD88 gene knockout may attenuate paraquat-induced ALI and reduce the production of proinflammatory cytokines.

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