Locatelli F.,Istituto di Ricovero e Cura a Carattere Scientifico |
Locatelli F.,University of Pavia |
Kabbara N.,Eurocord |
Ruggeri A.,Eurocord |
And 13 more authors.
Blood | Year: 2013
We analyzed the outcomes of 485 patients with thalassemia major (TM) or sickle cell disease (SCD) receiving HLA-identical sibling cord blood transplantation (CBT, n = 96) or bone marrow transplantation (BMT, n = 389). Compared with patients given BMT, CBT recipients were significantly younger (median age 6 vs 8 years, P = .02), and were treated more recently (median year 2001 vs 1999, P < .01). A higher proportion of patients with TM belonging to classes II-III of the Pesaro classification received BMT (44%) compared with CBT (39%, P < .01). In comparison with patients receiving BMT(n = 259, TM; n = 130, SCD), those given CBT (n = 66, TM; n = 30, SCD) had slower neutrophil recovery, less acute graft-versus-host disease (GVHD) and none had extensive chronic GVHD. With a median follow-up of 70 months, the 6-year overall survival was 95% and 97% after BMT and CBT, respectively (P = .92). The 6-year disease-free survival (DFS) was 86% and 80% in TM patients after BMT and CBT, respectively, whereas DFS in SCD patients was 92% and 90%, respectively. The cell dose infused did not influence outcome of patients given CBT. In multivariate analysis, DFS did not differ between CBT and BMT recipients. Patients with TMor SCD have excellent outcomes after both HLA-identical sibling CBT and BMT. © 2013 by The American Society of Hematology.
Pourmomeny A.A.,Isfahan University of Medical Sciences |
Zadmehre H.,Shariati Hospital |
Mirshamsi M.,Isfahan University of Medical Sciences |
Mahmodi Z.,Isfahan University of Medical Sciences
Otology and Neurotology | Year: 2014
OBJECTIVE: The aim of present study was to find a better procedure to prevent synkinesis in facial nerve palsy. DESIGN: A randomized clinical trial. SETTING: Clinical rehabilitation faculty and general rehabilitation centers. SUBJECTS: Twenty- nine patients with facial nerve palsy were selected via electrodiagnosis tests. INTERVENTION: The experimental group (n = 16) was treated with biofeedback electromyography, whereas the control group (n = 13) received common physiotherapy. All patients received 1-year treatment. MAIN OUTCOME MEASURES: Evaluation of facial grading scale before and after treatment and severity of synkinesia. RESULTS: At the end of treatment, there was a rise in facial grading system in both groups compared with baseline (p < 0.05, paired t test). There were significant differences in the facial grading system between 2 groups at the end of the study, but the experimental group showed better result than the other one. The number of patients with synkinesis and the severity of their synkinesis in the experimental group were lesser than those of the control group. CONCLUSION: Biofeedback therapy is more efficient than common physiotherapy. By using this approach, controlling and reducing synkinesis is more feasible. © 2014, Otology & Neurotology, Inc.
Alavi-Moghaddam M.,Imam Hossein University |
Safari S.,Imam Hossein University |
Najafi I.,Shariati Hospital |
Hosseini M.,Tehran University of Medical Sciences
European Journal of Emergency Medicine | Year: 2012
OBJECTIVES: To evaluate the utility of urine dipstick test (UDT) for detecting rhabdomyolysis and acute kidney injury (AKI) due to crush injury. METHODS: All the rescued victims of the Bam earthquake who had a documented urine analysis and serum creatine phosphokinase and creatinine levels during their hospitalization period were eligible to enter the study. The sensitivity and the specificity, along with the positive and negative likelihood ratios, of UDT in detecting at-risk patients for rhabdomyolysis and crush-related AKI were calculated. RESULTS: Urine red blood cell count of 5 or less in blood-positive UDT, as a surrogate marker for myoglobinuria, was reported in 210 (31.7%) of the total 1821 urine analyses. Blood-positive UDTs (without considering the urine red blood cell count) had a 92.5% (95% confidence interval: 79.6-98.4) sensitivity in creatine phosphokinase, with a cut-off of 15 000 (IU/l). Comparing the results of the serum creatinine level and the urine blood, analysis showed that UDT had a sensitivity and a specificity of as high as 83.3 and 56.6% in detecting high-risk patients for AKI, respectively. CONCLUSION: UDT can be considered as an early screening tool for the detection and triage of patients at risk of developing AKI because of traumatic rhabdomyolysis after mass disasters. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Ghoraeian P.,Tarbiat Modares University |
Mozdarani H.,Tarbiat Modares University |
Aleyasin A.,Shariati Hospital |
Alizadeh-Nili H.,Tarbiat Modares University
Andrologia | Year: 2013
Chromosomal aneuploidy is a well-known phenomenon in human gametes including spermatozoa. Success rate of fertilisation and implantation in subfertile patients with male factor has always been shown to be very low. We tried to relate the possible impact of sex chromosomal aneuploidy in spermatozoa used for intracytoplasmic sperm injection (ICSI) on fertilisation and implantation rate. To evaluate the frequency of disomy for X and Y chromosomes in sperm samples retrieved from normal and oligozoospermic individuals, primed in situ labelling (PRINS) technique was used. Following ICSI, the rate of eight-cell embryos for each category was determined and followed up for successful implantation. Results showed a statistically significant higher frequency of disomy for all chromosomes under study in spermatozoa of oligozoospermic patients compared with normal men (P < 0.01). The rate of eight-cells embryo formation was significantly lower than in normal group (P < 0.01). The number of embryos transferred for both groups were nearly similar. Implantation rate for oligozoospermic patients was much lower than that of the normal group but was not significantly different (P > 0.05). These results demonstrate that men especially with severe oligozoospermia have an elevated risk for chromosome abnormalities in their spermatozoa. These abnormalities might affect fertilisation and pre-embryo formation with less impact on implantation. © 2012 Blackwell Verlag GmbH.
Moghbeli-Nejad S.,Tarbiat Modares University |
Mozdarani H.,Tarbiat Modares University |
Aleyasin A.,Shariati Hospital
Journal of Assisted Reproduction and Genetics | Year: 2012
Purpose: Induced chromosomal instability and micronucleus (MN) formation in blood lymphocytes of infertile men in comparison with fertile men exposed to gamma radiation was investigated. Methods: Blood samples of healthy and infertile donors were irradiated by 2 and 4 Gy Co-60 gamma-rays, then cultured in RPMI-1640 complete medium containing 1% phytoheamaglutinin (PHA) and incubated in a CO 2 incubator. Cytochalasin-B was added to the cultures at a final concentration of 4 μg/ml. Finally, harvesting, slide making, and analysis were performed according to standard procedures. Results: We observed a statistically significant difference between the frequencies of micronuclei in lymphocytes of infertile individuals, compared to healthy donors, before and after exposure to gamma rays. Although higher in azoospermia patients, the frequency of MN was not statistically different between infertile groups. Conclusions: This study indicates that genomic instability in infertile men could probably contribute to the development of an impaired reproductive capacity. © 2011 Springer Science+Business Media, LLC.