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Alhussaini M.S.,Shaqra University
Medical Journal of Indonesia | Year: 2016

Background: Helicobacter pylori is an important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. The present study was carried out to determine the relationship between this organism with different gastrointestinal ailments. Methods: 150 outpatients referrals to Saudi Arabian Medical City, Riyadh, Kingdom of Saudi Arabia was recruited in January to June 2015. Each patient was subjected to endoscopic examination. Biopsy specimens were taken from the stomach for rapid urease test and culture. Suspected H. pylori colonies were subjected to colony morphology identification, microscopical examination and biochemical reactions. The samples were also subjected to PCR to detect ureA subunit of urease gene. Results: The endoscopic examination of patients revealed normal, gastric ulcer, duodenal ulcer, gastritis, and gastric cancer with a rate of 20.7%, 20%, 24%, 33.3%, and 2%, respectively. Direct smear exam revealed that 52% of patients were H. pylori positive while culture and rapid urease test showed a prevalence of 71.33%. Fifty four biopsies (36%) were urease positive after 1 hour at room temperature, 39 (62%) after 1 hour incubation at 37°C and 14 (71.33%) after 24 hours incubation. Isolated H. pylori showed that they were catalase, oxidase, and urease positive. PCR results showed 411-bp fragment, which is indicative for the ureA subunit of urease gene. Conclusion: The prevalence of H. pylori infection was high among tested population. Strong association between H. pylori and duodenal ulcer was noticed. A 411-bp fragment indicative of the ureA subunit of urease gene was detected in all the tested isolates. © 2016 Authors.

Kuddus M.,Integral University | Kuddus M.,Shaqra University | Ramteke P.W.,SHIATS
Critical Reviews in Microbiology | Year: 2012

Microbial proteases that occupy a pivotal position with respect to their commercial applications are most important hydrolytic enzymes and have been studied extensively since the advent of enzymology. Cold-adapted microorganisms are potential source of cold-active proteases and they have been isolated from the cold regions. Although there are many microbial sources available for producing proteases, only few are recognized as commercial producer. Cold-active proteases along with their producing microbes are of commercial value and find multiple applications in various industrial and biotechnological sectors such as additives in detergents, additives in food industries, environmental bioremediations, biotransformation and molecular biology applications. Therefore, cold-active proteases are the enzymes of choice for many biotechnologists, microbiologists, biochemists, environmentalists and biochemical engineers. In the present review, we discuss some novel sources along with recent developments in production and biotechnological applications of cold-active microbial proteases. © 2012 Informa Healthcare USA, Inc.

El-Nahass M.M.,Ain Shams University | Hassanien A.M.,Shaqra University
Radiation Physics and Chemistry | Year: 2014

The effect of γ-irradiation (6. kGy) on the optical properties of thermally evaporated perylene-66 (dye content 40%) thin films has been reported. The optical constants (refractive index, n, and absorption index, k) of the as-deposited and γ-irradiated films have been obtained in the wavelength range 200-2500. nm using spectrophotometric measurements at nearly normal incidence. The obtained optical constants were used to estimate the type of transition for the as-deposited and irradiated films. The single oscillator model and Drude model of free carriers' absorption were used for the analysis of refractive index dispersion, in the normal dispersion range, before and after γ-irradiation. © 2013 Elsevier Ltd.

Al-Rohaimi A.H.,Shaqra University
Journal of Oleo Science | Year: 2015

The problem of poor bioavailability and clinical efficacy of curcumin can be sorted out after converting crystalline Curcumin (CrysCur) into amorphous NanoCurcumin (NanoCur). Amorphous NanoCur was prepared by converting into nanoemulsion (o/w) using water titration method. The formulation were pre-screen by different physical stress tests, followed by in vitro release study, zeta potential, viscosity, transmittance, globule size distribution and ex vivo studies. The morphology of the NanoCur was determined using transmission electron microscopy (TEM) which revealed fairly spherical shape and good correlation with droplet size distribution study. The NanoCur was converted to gel using Cabopol 934. The composition of optimized NanoCur was curcumin (0.154% w/w), Carbopol 934 (0.702% w/w), ethanolic oil phase [ethanol (0.013% w/w): Capryol 90 (0.015%w/w)], Tween 20 (0.076%w/w) as surfactant, PEG 200 (0.038%w/w) as a co-surfactant and distilled water (q.s) as hydration phase. The steady state fux (Jss), permeability coeffcient (Kp) and enhancement ratio (Er) of NanoCur gel was determined and compared with CrysCur gel. Anti-inflammatory effects of the formulations were evaluated in carrageenan-induced paw edema method in rats using Diclofenac as a reference. These ant-infammatory effects of NanoCur was highly significant (p<0.001) compared to CrysCur and significantly (p<0.05) comparable with standard Diclofenac. The histology of the formulation treated skin showed insignifcant changes in the integrity except in the group treated with NanoCur. The slight disruption in the integrity of skin may be because of surfactant present in the nano formulations. Short term storage stability showed insignificant changes in the droplet size and zeta potential, proving its high shelf-life. Finally, it was concluded that NanoCur could be a promising tool in the management of topical infammation. © 2015 by Japan Oil Chemists’ Society.

Khan Z.F.,Shaqra University
Advances in Intelligent Systems and Computing | Year: 2016

A color image-based segmentation method for segmenting skin lesions is proposed in this paper. This proposed methodology mainly includes two parts: First, a combination of scale-invariant and semantic mathematic model is utilized to classify different pixels. Second, a strategy based on skeleton corner point’s extraction is proposed in order to extract the seed points for the skin lesion image. By this method, the skin slices are processed in series automatically. As a result, the lesions present in the skin can be segmented clearly and accurately. The proposed algorithm is trained and tested for 360 skin slices in order to evaluate the accuracy of segmentation. Overall accuracy of the proposed method is compared with existing conventional techniques. An average missing pixel rate of 3.02% and faulting pixel rate or 2.36% has been obtained for segmenting the skin lesion images. © Springer India 2016.

Al-Amri S.M.,Shaqra University
Saudi Journal of Biological Sciences | Year: 2013

A field experiment was conducted to investigate the effect of seed presoaking of shikimic acid (30, 60 and 120. ppm) on growth parameters, fruit productivity and quality, transpiration rate, photosynthetic pigments and some mineral nutrition contents of tomato plants. Shikimic acid at all concentrations significantly increased fresh and dry weights, fruit number, average fresh and dry fruit yield, vitamin C, lycopene, carotenoid contents, total acidity and fruit total soluble sugars of tomato plants when compared to control plants. Seed pretreatment with shikimic acid at various doses induces a significant increase in total leaf conductivity, transpiration rate and photosynthetic pigments (Chl. a, chl. b and carotenoids) of tomato plants. Furthermore, shikimic acid at various doses applied significantly increased the concentration of nitrogen, phosphorus and potassium in tomato leaves as compared to control non-treated tomato plants. Among all doses of shikimic acid treatment, it was found that 60. ppm treatment caused a marked increase in growth, fruit productivity and quality and most studied parameters of tomato plants when compared to other treatments. On the other hand, no significant differences were observed in total photosynthetic pigments, concentrations of nitrogen and potassium in leaves of tomato plants treated with 30. ppm of shikimic acid and control plants. According to these results, it could be suggested that shikimic acid used for seed soaking could be used for increasing growth, fruit productivity and quality of tomato plants growing under field conditions. © 2013 .

Sheik Abdulazeez S.,Shaqra University
Journal of Taibah University Medical Sciences | Year: 2014

Objectives Strawberries contain antioxidant phytochemicals, which have been shown to beneficial against inflammation, cardiovascular disease, cancer, obesity, neurodegeneration, hyperglycaemia and associated diabetic complications. Freeze-dried strawberry powder (FSP) is effective in controlling metabolic syndrome and cardiovascular risk factors. The present investigation was designed to evaluate the effect of FSP on alloxan-induced diabetes in rats.Methods Diabetes was induced in male Wistar albino rats by intraperitoneal injection of alloxan monohydrate (150 mg/kg bw), and treatment with FSP (700 mg/day for 45 days) was started 48 h after alloxan injection. Blood glucose, insulin, C-peptide, haemoglobin, renal function markers (urea, uric acid and creatinine) and enzyme markers (aspartate and alanine transaminases and alkaline phosphatase) were assayed with suitable methods.Results Animals given alloxan had significant body weight loss, poor glucose tolerance and increased blood glucose with concomitant decreases in insulin, C-peptide and haemoglobin values, and significant (p < 0.05) changes in the levels of renal function markers and enzyme activities. Oral administration of FSP for 45 days significantly (p < 0.05) reverted the body weight loss, hyperglycaemia, biochemical parameters, enzyme activities and insulin levels to near normal levels.Conclusions FSP can revert alloxan-induced diabetes and its complications. The antioxidant properties of the fruit probably play a major role in the observed changes. © 2014 Taibah University.

Reda A.M.,Shaqra University
Radiation Measurements | Year: 2011

Shielding for a D-T sealed neutron generator has been designed using the MCNP5 Monte Carlo radiation transport code. The neutron generator will be used in field for the detection of explosives, landmines, drugs and other 'threat' materials. The optimization of the detection of buried objects was started by studying the signal-to-noise ratio for different geometric conditions. © 2011 Elsevier Ltd. All rights reserved.

Akl A.A.,Shaqra University | Hassanien A.S.,Shaqra University
Superlattices and Microstructures | Year: 2015

Cadmium sulfoselenide CdSxSe1-x thin films were thermally evaporated onto preheated glass substrates (523 K). The evaporation rate and film thickness were kept constant at 2.5 nm/s and 375 ± 5 nm, respectively. Microstructure and crystal imperfections of deposit CdSxSe1-x thin films were studied using X-ray diffraction (XRD) and energy dispersive analysis by X-ray (EDAX). XRD analysis reveals the formation of films have the semi-crystalline nature and the hexagonal structure with preferential (0 0 2) direction. The microstructural parameters such as, lattice parameters, the crystallite size (D), microstrain (ε), residual internal stress (S), dislocation density (δ) and number of crystallite per unit volume (N) were calculated and found to be dependent upon the composition. The presence percentage of Cd, S and Se elements in the chalcogenide CdSxSe1-x thin films were estimated by EDAX and a comparative study with other similar samples of the previous literature was discussed. © 2015 Elsevier Ltd. All rights reserved.

Abel-Salam B.K.A.,Shaqra University
Allergologia et Immunopathologia | Year: 2012

Background: Alteration in the proliferation capacity of leukocytes and in the level of some cytokines, such as TNF-α, IL-4 and IL-8 have been suggested to associate with Diabetes mellitus in alloxan-induced diabetic rats given the potential immunomodulatory effects of black seeds and garlic. Aim of the work: The aim of this study was to test the effects of these agents on the immune cells in alloxan-induced diabetic rats. Methods: To this end, Diabetes was induced in albino rats by a single intraperitoneal injection of alloxan monohydrate (120. mg/kg of body weight). Diabetic rats were then fed normal diet or diet with black seeds or garlic for 28 days. Results: The results showed significant increase in the numbers of monocytes and granulocytes, but with significant decreases in lymphocyte proliferation and the TNF-α, interleukin (IL)-4 and IL-8 levels in the diabetic group. Treatment of diabetic rats with black seeds or garlic induced significant amelioration in the numbers of monocytes and granulocytes, with significant increase in lymphocytes numbers and the TNF-α, IL-4 and IL-8 levels. Conclusions: These results indicate the potential beneficial effects of black seeds and garlic as adjuvant treatment during treatment of Diabetes. © 2011 SEICAP.

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