Ma J.,Shaoxing Central Hospital |
Zhang X.-L.,Wenzhou University |
Wang C.-Y.,Wenzhou University |
Lin Z.,Wenzhou University |
And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015
Objective: To investigate the neuro-protective effects of dexmedetomidine (dex) on I/R-induced spinal injury and potential mechanisms. Methods: sprague-Dawley rats in the treatment group received intraperitoneal injections of 25 mg/kg dexmedetomidine, MC stabilizer cromolyn (100 mg/kg), MCs stimuliser compound 48/80 (80 mg/kg), PBS at 24 h befor IR. Underwent 5 minutes of aortic occlusion via median sternotomy, functional scores were recorded at 12, 24, 36 and 48 hours after reperfusion. Additionally, 3 mice underwent sham surgery with sternotomy and dissection of the aorta and subclavian artery with no occlusion. Spinal cords were examined for protein kinase B (AKT), CREB, and brain-derived neurotrophic factor (BDNF) following treatment alone or ischemiareperfusion surgery. Collected the serum to observe the expression of pro-inflammation cytokines (TNF-α, INF-γ and IL-1β) and anti-inflammation cytokines (TGF-β, IL-10 and IL-6). Then the MCs were harvested to test the expression surface molecular of FcεR and MCs’ degranulation. Results: Pretreated the rats with dexmedetomidine has higher neurologic function at all time points after I/R injury. We collected the serum of rats then detected the pro-inflammation cytokines TNF-α, INF-γ and IL-1β levels and anti-inflammation cytokinses TGF-β, IL-10 and IL-6 levels, found that the pro-inflammation cytokines of dexmedetomidine group was decreased whereas the anti-inflammation cytokinses was increased. At the same time the protect protein of AKT, CREB and mRNA BDNF were increased. They had the same results with cromolyn group, and opposite with the compound 48/80 group. We pretreated MCs with dexmedetomidine in vitro, and found that the activity surface molecular of MCs was down-regulation, and MCs degranulation was decreased. Conclusion: We thus demonstrate a possible mechanism by which dexmedetomidine alleviates spinal cord I/R injury through blocking the MCs degranulation. © 2015, E-Century Publishing Corporation. All rights reserved.
Zhang H.-J.,Shaoxing Central Hospital |
Lu C.-Q.,Shaoxing Central Hospital |
Fang C.-W.,Shaoxing Central Hospital
Chinese Journal of Contemporary Pediatrics | Year: 2015
Objective: To study the changes and significance of lymphocyte sunsets and serum interferon-γ (IFN-γ) levels in children with toxoplasma infection. Methods: Thirty-four children who were newly diagnosed with toxoplasma infection (TOX-IgM+ group) between January 2011 and April 2014, 12 children who had ever been diagnosed with toxoplasma infection (TOX-IgG+ group), and 54 healthy children (control group) were enrolled. The percentages of CD4+, CD8+, CD19+ and NK cells in peripheral blood lymphocytes were detected by flow cytometry. Serum levels of IFN-γ were measured using ELISA. Results: The percentages of CD4+ cells and the CD4+/CD8+ ratio in the TOX-IgM+ group were significantly lower than in the TOX-IgG+ and control groups, while the percentages of CD8+ and NK cells and serum IFN-γ levels were significantly higher than in the other two groups (P<0.05). The TOXIgG+ group had higher serum IFN-γ levels than the control group (P<0.05). There was a positive correlation between the percentage of CD8+ cells and serum IFN-γ levels in the TOX-IgM+ group (r=0.756; P<0.05). Conclusions: CD4+, CD8+ and NK cells may play important roles in the resistance against toxoplasma infection by promoting the secretion of cytokines. Copyright © 2015 Chinese Journal Of Contemporary Pediatrics.
Lu L.,Shaoxing Central Hospital |
Fang B.,Shaoxing Central Hospital |
Zhu D.,Shaoxing Central Hospital
International Journal of Clinical and Experimental Medicine | Year: 2016
One male patient at 59 years old of age was diagnosed as cervical myelopathy for unsteady gait and stiff lower limbs, followed by cervical 3/4 and 5/6 discectomy plus bone grafting and internal fixation, when processing the superior endplate of left cervical 4 disk, a large number of arterial bleeding occurred, which was stopped by packing method, and followed by continued cervical discectomy plus internal fixation. This patient was followed up for five years, the left vertebral artery was blocked, while no significant vascular injury-associated complication occurred, nor the symptoms of spinal cord further aggravated. We believed that the missed-diagnosed transverse foramen malformation before surgery led to the occurrence of almost catastrophic intraoperative bleeding, which might be ignored by the vast majority of clinicians and imaging physicians in preoperative routine magnetic resonance imaging (MRI) examination, therefore, paying high attention to this situation, as well as preoperative vertebral CT scanning or vertebral artery angiography would be essential. © 2016, E-Century Publishing Corporation. All rights reserved.
Chen J.,Shaoxing Central Hospital |
Fang S.-Y.,Shaoxing Central Hospital |
Fu Y.-P.,Shaoxing Central Hospital |
Fan C.-L.,Shaoxing Central Hospital
Journal of Practical Oncology | Year: 2015
To investigate the expression of junctional adhesion molecule A (JAM-A) in thyroid papillary carcinoma and to evaluate its clinicopathological significance. Sixty tissue samples of thyroid papillary carcinoma and 30 samples of normal thyroid glands were analyzed for JAM-A by immunohistochemistry. The expression of JAM-A was decreased in papillary carcinoma compared to normal thyroid tissues (P < 0.05). The expression of JAM-A in papillary carcinoma tissues with lymph node metastasis was lower than that in those without lymph node metastasis (P < 0.5). JAM-A expression in thyroid papillary carcinoma is down-regulated, particularly in papillary carcinoma tissues with lymph node metastasis. © 2015, Editorial Board of Journal of Practical Oncology. All right reserved.