Bai J.,Shanxi Medical University |
Lei P.,Shanxi Medical University |
Zhang J.,Shanxi Tumor Hospital |
Zhao C.,Shanxi Medical University |
Liang R.,Shanxi Medical University
Toxicology | Year: 2013
Although sulfite (SO32-) is commonly used as an antimicrobial agent and preservative in foods, medicines and wine, it has also been listed as an important risk factor for the initiation and progression of liver diseases due to oxidative damage. In general, apoptosis that is induced by oxidative stress is triggered by increases in p53 and alterations in Mdm2 and Bcl-2. However, the level of involvement of the p53 signaling pathway, which has been shown to be upregulated in some animal studies, in hepatocyte death remains unclear. To examine the response of the p53 signaling pathway to stimulation with different concentrations of sulfite, a time course study of p53, Mdm2, and Bcl-2 expression was conducted in an immortalized hepatic cell line, HL-7702. When the HL-7702 cells were cultured in the presence of Na2SO3, the cell viability was significantly decreased after 24h compared to that of the control group (0mmol/L) (p<0.05). Meanwhile, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in the supernatants of HL-7702 cells were significantly increased following Na2SO3 administration. Interestingly, the expression of p53 and p-p53 (Ser15) remained unchanged. In addition, no obvious alterations in Mdm2 and Bcl-2 expression were observed in HL-7702 cells that had been stimulated with various concentrations of sulfite. To further investigate the detailed mechanism underlying sulfite toxicity, caspase-3, PCNA and RIP1 expression in HL-7702 cells was studied. The expression levels of caspase-3 and PCNA were unchanged, but RIP1 expression was increased significantly after 24h of exposure. In light of this evidence, we propose that sulfite is cytotoxic to hepatocytes, but this cytotoxicity is not achieved by direct interruption of the p53 signaling pathway. In addition, we propose that an alternative necrotic process underlies hepatocellular death following sulfite exposure. © 2013 Elsevier Ireland Ltd.
Wang T.,Shanxi Medical University |
Gui W.,Shanxi Tumor Hospital |
Shen Q.,Shanxi Medical University
Medical Oncology | Year: 2010
The objective is to investigate the association between pathological type and clinical features, response to treatment and prognosis of primary gastrointestinal Non-Hodgkin's lymphoma (PGINHL). The clinicopathologic features, response to treatment and survival of 124 patients with PGINHL, were investigated retrospectively. Ninety-one patients were treated with surgery, most of which included combined therapy, while 32 patients received chemotherapy alone. Survival analysis was performed by Kaplan-Meier and Cox regression method. The most common immunophenotype was B-cell subtype. In 115 (92.7%) patients of B-cell lymphoma, mucosa-associated lymphoid tissue lymphoma (MALToma) and diffuse large B-cell lymphoma (DLBCL) were 55 and 50 patients, respectively. The patients of two pathological types had different clinical features including stage, B symptoms, sites of tumor, distant involvement, International Prognosis Index Score, size of tumor, and response to treatment. Both overall survival curve and multiple Cox regression analysis indicated that pathological type was statistically significant. The pathological subtype of PGINHL was an important prognostic factor. The patients with MALToma appear to have more favorable prognosis than those with DLBCL. © 2009 Humana Press Inc.
Shi Y.,Shanxi Tumor Hospital |
Liang X.,Shanxi Tumor Hospital
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2015
Imatinib is the key medication for adjuvant therapy in gastrointestinal stromal tumors(GIST) and the first line therapy for patients with metastatic or recurrent GIST. Preoperative treatment with imatinib may improve R0 resection rate and provide the chance of metastasectomy for cytoreduction as well as prolonging patient's survival. We investigate the significance of neoadjuvant therapy of imatinib and the timing of surgery by reviewing clinical trials and consensus in recent years.
Xi Y.,Huazhong University of Science and Technology |
Xi Y.,Shanxi Tumor Hospital |
Li J.,Shanxi Tumor Hospital |
Zan L.,Shanxi Tumor Hospital |
And 3 more authors.
Human Pathology | Year: 2013
The diagnosis and prognosis of T-lymphoblastic lymphoma/leukemia represent a clinical challenge due to their relative rarity as well as their heterogeneous morphology and immunophenotype. In contrast to the significant progress in uncovering genetic lesions and prognostic factors for B-cell lineage lymphoid malignancies, prognostic markers that can predict clinical outcome of T-lymphoblastic lymphoma/leukemia remain to be discovered and validated. Recently, specific micro-RNAs have emerged as potential biomarkers for the diagnosis and prognosis of various types of tumor. Among them, miR-16 is known to play important roles in the development of hematological malignancies. Using quantitative real-time reverse transcription-polymerase chain reaction, we analyzed miR-16 expression level in archived formalin-fixed and paraffin-embedded diagnostic lymph node specimens from a total of 72 patients with T-lymphoblastic lymphoma/leukemia and for which clinical follow-up information was available. Although no statistically significant difference was evident in patients with T-lymphoblastic lymphoma/leukemia versus controls with reactive lymph nodes (P =.163), T-lymphoblastic lymphoma/leukemia patients with a greater than median expression level of miR-16 had a longer overall survival than patients with a less than median expression level. The overall 1-year survival rate was 50% for the high expression group but was 26.5% for the low expression group (P =.043). To our knowledge, we provide here the first evidence that the expression of a single micro-RNA, miR-16, is associated with clinical outcome of patients with T-lymphoblastic lymphoma/leukemia. Therefore, miR-16 may be considered as a potential prognostic marker for T-lymphoblastic lymphoma/leukemia. © 2013 Elsevier Inc.
Zhang H.W.,Shanxi Tumor Hospital
Zhonghua zhong liu za zhi [Chinese journal of oncology] | Year: 2013
To study the relationship between myc gene rearrangement and myc protein expression in diffuse large B cell lymphoma (DLBCL), and their correlation with prognosis. One hundred and six cases of DLBCLs with follow-up data were analyzed using interphase fluorescence in situ hybridization (FISH) technique. Immunophenotyping analysis for CD20, CD3, myc, Mum-1, CD10, bcl-6 was also performed using EnVision immunohistochemistry. The percentages of tumor cells expressing myc, Mum-1, CD10 and bcl-6 were 70.8%, 56.6%, 21.7% and 26.4%, respectively. Twenty six cases (24.5%) were of GCB type and the rest (75.5%) were of non-GCB (non germinal center) type. The myc rearrangement was identified in 13 (12.3%) of 106 cases. 13 cases showed to be of non-GCB type. There was no correlation between myc rearrangement and myc protein expression. DLBCLs (n = 13) with myc rearrangement showed significantly poorer overall survival (OS) and progression free survival (PFS), with a median OS and PFS time of 4.7 and 3.2 months, respectively (for OS and PFS, P < 0.001). Multivariate analysis using Cox proportional hazard model confirmed that myc rearrangement, ECOG performance status of 2-4, immunophenotyping subgroup and myc protein were independent factors affecting the prognosis and significantly associated with the survival. However, myc rearrangement was the strongest prognostic factor. DLBCL with myc gene rearrangement is a subgroup of non-GCB DLBCL with poor outcome. It is an independent and useful factor for prognosis in DLBCL. Expression of myc is influenced by many factors and myc rearrangement may be one of these factors.
Li C.Y.,Shanxi Tumor Hospital
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2012
To explore the relationship between c-kit and platelet-derived growth factor receptor alpha(PDGFRA) gene mutation features and the prognosis of gastrointestinal stromal tumor(GIST). Clinicopathological, genetic testing and follow-up informations of patients admitted to the Shanxi Tumor Hospital from June 2000 to January 2009 were collected. The survival was calculated and univariate analysis was conducted using the Kaplan-Meier method. Multivariate analysis was conducted by the Cox regression method. The 5-year disease-free survival rate was 61.5% and the 5-year overall survival rate was 67.4%. The 5-year disease-free survival rates of patients without disease among those with c-kit exon 11 mutation (n=77), c-kit exon 9 mutation(n=4), and PDGFRA exon 18 mutation (n=2) were 63.4%, 14.3% and 100%, and the 5-year overall survival rates were 70.8%, 50.0% and 100%, respectively. In the patients with c-kit exon 11 mutation, the 5-year disease-free survival rates among those with point mutations(n=26), deletion mutations(n=44), and duplication mutations(n=7) were 87.1%, 44.9% and 80.0%, and the 5-year overall survival rates were 88.1%, 57.0% and 100%, respectively. There were significant differences in overall survival among different factors. Multivariate analysis showed that gene mutation was not the independent factor of prognosis(P=0.492). In GIST patients undergoing surgery without imatinib treatment, mutated genotype is better than wild type in terms of prognosis. Gene mutation is not the independent factor of prognosis in GIST patients.
Zhang L.,Shanxi University |
Li Z.,Shanxi University |
Fan Y.,Zhejiang Chinese Medical University |
Li H.,Shanxi University |
And 2 more authors.
International Journal of Biochemistry and Cell Biology | Year: 2015
Glucose-regulated protein of 78 kD (GRP78) is a multifunctional protein belonging to the heat shock protein 70 family. Overexpression of GRP78 triggered by environmental and physiological stresses is positively correlated with the occurrence and progression of various tumors, but the molecular mechanisms have not been well established. The present study indicated that overexpression of GRP78 in colon cancer cells could promote cell-matrix adhesion through the upregulation of fibronectin, integrin-β1 and phosphorylated FAK. Meanwhile, it resulted in a visible epithelial-mesenchymal transition in DLD1 cells, and the Snail-2 played the key role during the process. More importantly, the data indicated that GRP78 overexpression facilitated the expression and secretion of TGF-β1, which further activated the downstream Smad2/3 signaling module to effectuate the cell-matrix adhesion and epithelial-mesenchymal transition. Taken together, this study provides a novel molecular mechanism involving in the effects of GRP78 on colon cancer metastasis. © 2015 Elsevier Ltd.
Liu X.,Shanxi Tumor Hospital
Chinese Journal of Oncology | Year: 2015
Objective: To analyze the clinicopathologic features and prognosis of α-fetoprotein (AFP)-producing gastric: cancers (AFPGC). Methods: Fifty-one serum AFP-positive patients with positive immunohistochemical staining of AFP in the primary lesions (study group) and sixty-five gastric cancer cases with normal AFP level (control group) treated in our department from January 2005 to December 2007 were included in this study. Their clinicopathologic features and follow-up data were statistically analyzed. Results Compared with the control group, the study group had a higher incidence of poorly differentiated adenocarcinoma (P =0. 021) and liver metastasis (P = 0. 001) than that in the control group. The TNM stages in the study group were significantly higher than those in the control group (P =0.001). The 1-, 2-, and 5-year survival rates of the study group were 62. 7%, 27. 5% and 4. 7%, respectively, and the median survival was 16 months, significantly lower than the 84. 6%, 55.4%, 16. 5%, and 30 months of the control group (P<0. 001 for all). The serum AFP levels in the study group ranged from 58. 63 μg/L to 12 100.00 μg/L, and could be classified into two groups:27 cases <500 μg/L, and 24 cases 5:500 μg/L. There was no significant difference of the immunohislochemical staining results between the two subgroups (P = 0.912). Conclusions: AFPGC is a special type of gastric cancer with high degree of malignancy and poor prognosis. Monitoring of serum AFP level can earlier detect the progression of disease and give corresponding treatment.
Wei J.,Shanxi Tumor Hospital |
Zhu Y.,Shanxi Tumor Hospital |
Xu G.,Shanxi Tumor Hospital |
Yang F.,Shanxi Tumor Hospital |
And 3 more authors.
Cell Biochemistry and Biophysics | Year: 2014
Oxymatrine, one of the most active components of the ethanol extracts from Sophora flavescens, is known for its potent antitumor activity both in vitro and in vivo. However, the mechanism of its action in mediating the cell apoptosis remains elusive. In this study, we investigated the proliferation inhibitory and apoptotic activities of oxymatrine against human osteosarcoma MG-63 cells. The compound was found to markedly and dose-dependently inhibit the cell proliferation determined by 5-bromo-2-deoxyuridine incorporation. Oxymatrine also induced the cell apoptosis in a dose- and time-dependent manner as showed by the annexin V-FITC/PI double staining and TUNEL assay. Furthermore, a disruption of mitochondrial membrane potential and an up-regulation of cleaved caspases-3, and-9 and downregulation of Bax/Bcl-2 was evidenced in the oxymatrine-treated cells. These proteins have been known to play a pivotal role in the regulation of apoptosis. In conclusion, these observations indicate of the oxymatrine potential as an effective antitumor agent against osteosarcoma. Moreover, the compound appears to exert its anti-tumor action by stimulating the caspase-triggered signaling pathway. © 2014, Springer Science+Business Media New York.
Liang X.B.,Shanxi Tumor Hospital
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2013
Surgery is the only possible cure protocol of gastrointestinal stromal tumor (GIST). But the risk of recurrence exists constantly. Risk assessment of relapse is very important to guide the targeted adjuvant therapy and predict the prognosis. Although the variables and grading in the risk assessment of recurrence after complete resection of primary local GIST have been identified, but either the F/NIH consensus, AFIP standards, modified NIH standards, or risk identification methods attempted to apply mathematical calculation model in recent years, including Jason S Gold risk nomogram, Rossi nomogram, Joensuu high Hotline Dengjun, are short of long-term, large-scale clinical trials without selection bias. Therefore, recurrence risk probability cannot be predicted accurately.