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Niu L.,Shanxi Medical University | Han D.W.,Shanxi Medical University | Xu R.L.,Shanxi Medical University | Han B.,Shanxi Medical University | And 5 more authors.
Journal of Nutrition, Health and Aging | Year: 2016

Objectives: Cases of sporadic Alzheimer’s disease (SAD) are the predominant form of the age-related dementia. New evidence suggests that metabolic syndrome (MS), a metabolic disorder, is an initiating factor of some SAD cases. A high-sugar high-fat diet could cause MS, we aimed to investigate whether it could directly lead to SAD. Measurements: The characteristic molecules of AD (hippocampus Aβ and Tau) were tested by using ELISA and western blotting to confirm the happening hallmarks of AD in brain. MS and inflammation related biochemical indicators were measured using immunological method. Proteins associated with the insulin resistance signal pathway (JNK, PI-3K, AKT, GSK-3β, GLUT3) were evaluated using western blotting method. The levels of reactive oxygen species (ROS) were measured by immunofluorescence method. Results: Expressions of hippocampus Aβ, phosphorylation-Tau (p-Tau), inflammatory factors and p-JNK, Gsk-3βwere higher in the model rats than those in the control rats and expressions of p-PI3K, p-AKT and GLUT3 were reversed. Conclusions: The MS model animals, which can induce the characteristics symptoms of AD, and therefore it may be preliminarily considered that the AD pertains to the MS-related diseases. © 2016, Serdi and Springer-Verlag France.

Han F.,Shanxi Provincial Tumor Hospital
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2012

To explore the relationship between esophagus cancer patients and both environmental and genetic factors, through analyzing the data on birth orders from esophagus cancer patients of Shanxi province. Both Greenwood and Haldane methods on birth order were used to study the 1101 cases with esophagus cancer from Shanxi province. All the patients had received surgery and were diagnosed, by pathological evidence. First certificates of the patients were confirmed through the standard genetic epidemiologic investigation. Birth order was investigated on probands of the 1101 cases with esophagus cancer and their 44 siblings. form the Greenwood method showed that there was a tendency for cases with esophagus cancer in birth orders First to Third. However, the Haldane method showed that the results were quite different between actual value and the average theory value of 6A (6A((actual value)) = 17 118, X -(6A(average theory value)) = 19 290, X = |6A-X -(6A)|/V(6A) = 7.63, X > 2) which suggested that the birth order had some effects on the occurrence of esophagus cancer. In addition, the actual value of 6A was lower than the theoretic average value, and the parents at younger productive age or baby at the first birth was easy to develop esophagus cancer. Esophagus cancer was related with the birth order, especially at early order, which was not consistent with the national reports on esophagus cancer. from this study suggested that there were certain effects of environmental risk factors on esophagus cancer patients.

To assess the effects of ischemic postconditioning, remote ischemic postconditioning and naloxone postconditioning on focal cerebral ischemia-reperfusion injury in rats. A total of 110 adult SD rats were randomly divided into 5 groups (n = 22 each). The focal cerebral ischemia-reperfusion injury was induced by a 90-minute occlusion of right middle cerebral artery (MCA) and a 24-hour reperfusion sequentially. Group 1 was of ischemia-reperfusion control; Group 2 ischemic postconditioning induced by three 30-second cycles of MCA occlusion followed by a 30-second reperfusion; Group 3 remote ischemic postconditioning performed via a transient occlusion of right femoral artery at 5 min before the initiation of reperfusion; Group 4 naloxone postconditioning with naloxone 10 mg/kg intraperitoneally injected at the initiation of reperfusion; Group 5 combined ischemic, remote ischemic & naloxone postconditioning performed simultaneously in accordance with the methods used in Groups 2, 3 & 4. The neurologic deficit scores (NDS) were obtained at 2 h & 24 h post-reperfusion. At 24 h post-reperfusion, the anesthetized rat was sacrificed by decapitation and the brain rapidly extracted to assess the size of cerebral infarct (n = 10), detect the cerebral expression of microtubule-associated protein-2 (MAP2) (n = 6), measure the plasma volume of cerebral tissues and quantify the diameter and segment length of cerebral microvessel (n = 6). There were no significant differences in the heart rate (HR) and mean arterial pressure (MAP) among the above five groups at all observed time points (P > 0.05). At 24 h post-reperfusion, the percentage of ischemic cerebral infarct size was 43% ± 6%, 31% ± 4%, 32% ± 5%, 28% ± 6% & 21% ± 7% in ipsilateral hemisphere area (i.e., cerebral infarct severity) in Groups 1-5 respectively. Compared with Group 1, the levels of NDS and cerebral infarct severity significantly decreased at ischemic side in Groups 2-5 (P < 0.05). And the cerebral expression of MAP2, plasma volume of cerebral tissues, diameter and segment length of cerebral microvessel significantly increased at the ischemic side (all P < 0.05). However, there were no significant differences in the above-mentioned parameters at ischemic side among Groups 2, 3 and 4 (all P > 0.05). The parameters of NDS, cerebral infarct severity, cerebral expression of MAP2 and plasma volume of cerebral tissues in the ischemic side significantly increased in Group 5 compared with Groups 1, 2, 3 and 4 (all P < 0.05). The diameter and segment length of cerebral microvessel at ischemic side were not different among Groups 2, 3, 4 and 5 (all P > 0.05). In focal cerebral ischemia-reperfusion rats, ischemic, remote ischemic and naloxone postconditioning may produce significant neuroprotective effects of reduced cerebral infarct severity and improved neurologic dysfunctions. A combination of three postconditioning approaches enhances the above neuroprotective effects.

Liu J.,Huazhong University of Science and Technology | Tian D.-A.,Huazhong University of Science and Technology | Wang J.-P.,Shanxi Provincial Peoples Hospital | Zhang S.-Z.,Shanxi Provincial Tumor Hospital | And 4 more authors.
Chinese Medical Journal | Year: 2011

Background The relationship between melanosis coli (MC) and aquaporin 8 (AQP8) has not yet been elucidated. The aim of this research was to investigate the relationship between the expression of AQP8 and the pathological mechanism of MC. Methods Expression of AQP8 was detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) in 37 MC colon tissues and 13 control colon tissues. Global gene expression analysis was also used to identify differently expressed genes. Its relationship with MC was analyzed by SPSS 11.5 statistical software. Results The positive rate of AQP8 expression detected by immunohistochemistry in the MC group was 24.3% (9/37), significantly lower than the 69.2% (9/13) in the control group (P <0.05). The relative expression level of AQP8 in MC group was 0.639±0.160, lower than 0.921±0.148 of controls (P <0.05). Global gene expression analysis showed that AQP8 mRNA expression was downregulated in MC patients. Conclusions The decreased AQP8 expression in MC patients indicates that chronic use of laxatives containing anthraquinone may cause reduced water absorption. The expression of AQP8 may be related to MC.

Sun L.,Shanxi Provincial Peoples Hospital | Yao J.-T.,Shanxi Provincial Tumor Hospital | Wang Y.-Y.,Shanxi Provincial Tumor Hospital | Li J.,Shanxi Provincial Tumor Hospital | Yuan R.,Shanxi Provincial Tumor Hospital
Chinese Journal of Cancer Prevention and Treatment | Year: 2013

OBJECTIVE: To study the inhibitory effect of mistletoe alkali on human lung adenocarcinoma carcinoma cell line SPC-A1 and lung squamous carcinoma cell line SK-MES-1 in vitro. METHODS: The inhibitory effect on the cells treated by mistletoe alkali with different concentration was evaluated by methyl thiazolyl tetrazolium (MTT) assay for 24 and 48 hours respectively. The cells treated by 5-fluorouracil (5-FU) were used as positive control group, while the cells without any treatment as negative control group. Different doses of mistletoe alkali were used as experimental group. The 50% inhibiting concentration (IC50) was assessed by cell growth curve. The rate of apoptosis for 24h was detected by Flow Cytometry. RESULTS: Mistletoe alkali did exert inhibitory effect on growth of cells in dose and time dependent manner. IC50 of mistletoe alkali to SPC-A1 and SK-MES-1 for 24 h was (14.43±0.53) μg/mL and (8.09±0.40) μg/mL respectively; IC50 was (12.11±0.25) μg/mL and (6.43±0.33) μg/mL respectively for 48 h. IC50 of 5-FU to SPC-A1 and SK-MES-1 for 24 h were (4.79±0.45) μg/mL and (5.15±0.23) μg/mL respectively; for 48 h were (4.35±0.41) μg/mL and (4.11±0.38) μg/mL respectively; Apoptosis rate was (0.43±0.01)% in control group, (31.09±0.05)% for SPC-A1 cell line in high dose group, the concentration of mistletoe alkali was 28.86 μg/mL, and (18.19±0.02)% in middle dose group, the concentration of mistletoe alkali was 14.43 μg/mL, (7.99±0.05)% in low dose group, the concentration of mistletoe alkali was 7.22 μg/mL. While for SK-MES-1 cell line, the apoptosis rate was (38.24± - 0.03)% in high dose group, the concentration of mistletoe alkali was 16.18 μg/mL, and (21.81±0.01)% in middle dose group, the concentration of mistletoe alkali was 8.09 μg/mL, (9.11±0.01)% in low dose group, the concentration of mistletoe alkali was 4.05 μg/mL. Compared with that of negative control group, mistletoe alkali led to dramatic increase in apoptosis rate of SPC-A1 and SK-MES-1 cell line (P<0.05), which was growing as the concentration increasing (P<0.05). CONCLUSIONS: Mistletoe alkali can inhibit the proliferation and induce apoptosis of SPC-A1 and SK-MES-1 cell line obviously, which provides an experimental reference for application of mistletoe alkali to treatment of human lung carcinoma.

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