Zhang T.,Shanxi Medical University |
Duan Z.,Shanxi Provincial Peoples Hospital
Cancer Research and Clinic | Year: 2016
Malignant tumor is a serious threat to human life, with an increasing incidence and mortality rate year by year. Thrombosis is the second leading cause of death in malignant tumor patients. Microparticles (MP) are cell vesicles derived from a variety of cells that are under the stimulation or apoptosis. The expression of tissue factor (TF) on the surface can he called TF positive MP, which participate not only in thrombosis, hut also in the invasion and metastasis of tumors. Therefore, this article will review the role of TF positive MP in patients with malignant tumors.
He M.,Shanxi Provincial Peoples Hospital |
Xue Y.,Shanxi Provincial Peoples Hospital
OncoTargets and Therapy | Year: 2017
Lung cancer has the highest morbidity and mortality in the world, and non-small cell lung carcinomas (NSCLC) account for 80% of cases of lung cancer. The mechanism of NSCLC is still largely unknown, and finding novel targets is of great importance for the treatment of NSCLC. The current study was designed to evaluate the role of miR-148a in NSCLC cell proliferation and invasion and to investigate the possible molecular mechanisms. We found that miR-148a expression was decreased in NSCLC tissues and cell lines. Upregulation of miR-148a significantly decreased A549 cell proliferation, and downregulation of miR-148a significantly increased A549 cell proliferation. Upregulation of miR-148a markedly increased apoptotic cell death and inhibited cell invasion potential. Upregulation of miR-148a significantly decreased signal transducer and activator of transcription 3 (STAT3) expression and 3′-untranslated region luciferase activity. Downregulation of miR-148a significantly increased STAT3 expression. Overexpression of STAT3 significantly inhibited the effect of miR-148a on cell viability and invasion potential. In conclusion, we found that miR-148a inhibited NSCLC cell proliferation and invasion potential through the inhibition of STAT3. Our findings highlight miR-148a/ STAT3 axis as a novel therapeutic target for the inhibition of NSCLC growth. © 2017 He and Xue.
Zi-Sheng A.,Tongji University |
You-Shui G.,Shanghai JiaoTong University |
Zhi-Zhen J.,Shanxi Provincial Peoples Hospital |
Ting Y.,Shanghai JiaoTong University |
Chang-Qing Z.,Shanghai JiaoTong University
Journal of Arthroplasty | Year: 2012
Current updated meta-analysis was designed to compare clinical effects of hemiarthroplasty (HA) vs primary total hip arthroplasty (THA) for displaced femoral neck fractures in elderly patients. Five randomized and 4 quasi-randomized controlled trials with a total 1208 patients were included for final analysis. It showed that mortality and postoperative infection between HA and THA had no statistical differences, that long-term reoperation rate of HA was higher than that of THA, that medium-term dislocation rate of HA was lower than that of THA, and that pain rates of HA in short-term and long-term were both higher than THA. Summarily, treatment of THA for elderly displaced femoral neck fracture could provide better results of reduced reoperation rate and pain relief; however, HA yielded a lower incidence of postoperative dislocation. © 2012 Elsevier Inc.
Hao Y.-X.,Shanxi Medical University |
Wang J.-P.,Shanxi Provincial Peoples Hospital |
Zhao L.-F.,Shanxi Medical University
Asian Pacific Journal of Cancer Prevention | Year: 2013
Aim: Associations between polymorphisms in miR-146aG>C, miR-196a2C>T and miR-499A>G and risk of HCC, and interaction with HBV infection in a Chinese population, were the target of the present research. Methods: The duplex polymerase-chain-reaction with confronting-two-pair primers (PCR-RFLP) was performed to determine the genotypes of the miR-146aG>C, miR-196a2C>T and miR-499A>G genotypes. Associations of polymorphisms with the risk of HCC were estimated by conditional logistic regression analysis. Results: Drinking, family history of cancer, HBsAg and HCV were risk factors for HCC. Multivariate regression analyses showed that subjects carrying the miR-196a2 CC genotype had significantly increased risk of HCC, with an adjusted OR (95% CI) of 2.18 (1.23-3.80). In addition, cases carrying the miR-196a2 C allele had a 1.64-fold increase in the risk for HCC (95%CI=1.03-2.49). The miR-196a2 CT and TT genotypes greatly significantly increased the risk of HCC in subjects with HBV infection, with adjusted ORs (95% CI) of 2.02 (1.12-3.68) and 2.69 (1.28-5.71), respectively. Conclusion: Our results demonstrate that miR-196a2 CC genotype and C allele have an important role in HCC risk in Chinese, especially in patients with HBV infection.
Liang L.,Shanxi Provincial Peoples Hospital |
Zhang Z.,Shanxi Provincial Traditional Chinese Medicine Institute
Cellular Physiology and Biochemistry | Year: 2016
Background/Aims: Malignant melanoma has high metastatic potential, is highly resistant to chemotherapy, and has a poor survival rate. Gambogic acid (GA), a polyprenylated xanthone extracted from a traditional Chinese medicinal herb, has been proven to exhibit antitumor activity. The present study aimed to investigate the signaling pathways that mediated GA-induced inhibition of human malignant skin melanoma proliferation. Methods: The study was conducted using A375 cells and the corresponding tumor transplanted in nude mice. Results: Incubation of A375 cells with 1-10 μg/ml GA decreased cell viability and increased apoptosis. GA concentration-dependently increased p66shc expression and intracellular ROS levels. GA also decreased the oxygen consumption rate and the mitochondrial membrane potential (MMP) in A375 cells. Experimental inhibition of p66shc by siRNA suppressed GA-induced increase of ROS, decrease of oxygen consumption rate, MMP and cell viability, whilst suppressing GA-induced increase of apoptosis. GA concentration-dependently upregulated p53 and Bax expression in A375 cells. GA also increased p53-TA-luciferase activity and p53-binding to Bax promoter, which was inhibited by Sip53. Experimental inhibition of p53 with Sip53 blocked GA-induced decrease of the oxygen consumption rate and cell viability, and blocked the increase of apoptosis. In tumor-bearing nude mice, GA notably inhibited tumor growth, and this action was suppressed by N-acetylcysteine (NAC), a potent antioxidant, and by PFT-α, a p53 inhibitor. In A375 tumors transplanted in nude mice, GA increased both p66shc and p53 expression. NAC and PFT-α treatment did not significantly affect p66shc expression in tumors grown in mice treated with GA. In contrast, both NAC and PFT-α treatment inhibited GA-induced p53 expression in mouse tumors. Conclusion: Results provided novel preclinical insights into the chemotherapeutic use of GA by highlighting the importance of p66shc/ROS-p53/Bax pathways in the antitumor effect of GA in malignant melanoma. © 2016 The Author(s) Published by S. Karger AG, Basel.
Wei H.,Shanxi Provincial Peoples Hospital |
Mori S.,Johns Hopkins University |
Hua K.,Johns Hopkins University |
Li X.,NY State Institute for Basic Research in Developmental Disabilities
International Journal of Developmental Neuroscience | Year: 2012
Abnormal neuroimmune responses have been reported to be associated with autism and could be appropriate targets for pharmacologic intervention. Our previous studies showed that neuroimmune factor, interleukin (IL)-6, was significantly elevated in the fontal cortex and cerebellum of autistic subjects. The IL-6 overexpressing mice displayed several autism-like features as well as an abnormal dendritic spine morphology and synaptic function. The purpose of this study was to examine the volumetric differences in the brain of IL-6 overexpressing mice and compare with corresponding control mice using magnetic resonance imaging. Here we show that IL-6 overexpressing mice display an increase in the total brain volume. In addition, the lateral ventricle is also enlarged in the IL-6 overexpressing mice. The brain structures surrounding the lateral ventricle were squeezed and deformed from the normal location. These results indicate that IL-6 elevation in the brain could mediate neuroanatomical abnormalities. Taking together with our previous findings, a mechanism by which IL-6 may be involved in the pathogenesis of autism is proposed. © 2012 ISDN.
Liao H.,Shanxi Provincial Peoples Hospital
Evidence-based Complementary and Alternative Medicine | Year: 2015
In Chinese medicine, Shengjiang (Zingiberis Rhizoma Recens) and its processed products, such as Ganjiang (Zingiberis Rhizoma), Paojiang (Zingiberis Rhizoma Preparatum), and Jiangtan (Zingiberis Rhizoma Carbonisata), exert distinct efficacy clinically. This research tried to study the effects of extracts from Shengjiang and its processed products in RAW 264.7 macrophage cells. After incubation of the different ginger types in RAW 264.7 cells for 24 h, an aliquot of the culture was mixed with an equal volume of Griess reagent, and nitric oxide (NO) production was evaluated using a Griess assay. Lipopolysaccharide (LPS) was used as the positive control. Milli-Q water (MQW) was used as the solvent control. The results showed that NO production increased significantly in RAW 264.7 cells following the stimulation of LPS (0.05 μg mL-1), Shengjiang, Ganjiang, Paojiang, and Jiangtan (50 μg mL-1, 500 μg mL-1) separately compared with the MQW control (P<0.01). The stimulation effects of Shengjiang and Ganjiang were significantly higher than those of Paojiang and Jiangtan at different concentrations (P<0.01). The conclusion we could get from this research is that Shengjiang and its processed products could induce NO production in RAW 264.7 cells. © 2015 Hui Liao.
Li T.-L.,Shanxi Provincial Peoples Hospital |
Zhang S.-Y.,Shanxi Provincial Peoples Hospital |
Du Y.-C.,Shanxi Provincial Peoples Hospital |
Yang P.-C.,McMaster University
Analytical Biochemistry | Year: 2013
The epithelial barrier dysfunction is associated with the pathogenesis of a number of diseases. Ubiquitin E3 ligase A20 (A20) plays a critical role in maintaining the homeostasis in the body. This study aimed to investigate the role of A20 in the degradation of endocytic antigens in airway epithelial cells. The expression of A20 in the human nasal epithelial cell line, RPMI 2650 cells (Rpcs), was evaluated. The role of A20 in maintaining the intracellular permeability in Rpc monolayers was assessed in Transwells. The endosome/lysosome fusion in epithelial cells was observed by immunocytochemistry. On the absorption of antigen, the expression of A20 was increased in Rpcs. The knockdown of the A20 gene in Rpcs increased the amounts of the endocytic antigens across the Rpc monolayers. A20 was required in the process of the endosome/lysosome fusion. The antigens transported to the basal compartment by A20-deficient Rpc monolayers still kept strong antigenicity. The nasal epithelial cell line, Rpcs, expresses A20 that facilitates the degradation of endocytic antigens in Rpcs by facilitating the endosome/lysosome fusion. © 2012 Elsevier Inc. All rights reserved.
Liao H.,Shanxi Provincial Peoples Hospital
Zhonghua nan ke xue = National journal of andrology | Year: 2012
Great progress has been made in the basic researches on erectile dysfunction (ED) ever since the recognition of the close association of micromolecular nitric oxide (NO) with penile smooth muscle relaxation in 1990. NO-cGMP-PDE5 signaling pathway plays an important role in regulating the relaxation of corpus cavernosum smooth muscle, and its relevant studies have contributed greatly to the clinical treatment of ED. Chinese herbal drugs have long been used in the treatment of ED, and the action mechanisms of some of them clarified through the NO-cGMP-PDE5 signaling pathway. This article presents an overview on the recent advances in the studies of ED treatment with Chinese herbal drugs.
Qin Q.,Shanxi University |
Qin Q.,Shanxi Provincial Peoples Hospital |
Qin S.,Shanxi University |
Wang L.,Shanxi University |
Lei W.,Shanxi University
Aquatic Toxicology | Year: 2012
Cadmium (Cd) is one of the most toxic heavy metals that can impact immunological parameters in aquatic animals. To investigate the immunotoxicity and ultrastructural changes of hemocytes, specimens of Sinopotamon henanense were exposed to different concentrations of cadmium and the differences in immunologic parameters between Cd exposure groups and control groups were investigated. Total hemocyte count (THC) in Cd-exposure groups were decreased significantly when compared with the control groups, especially in the groups treated with higher Cd concentrations and longer exposure time, while no significant differences were observed in the proportions of the three types of hemocytes. Phenoloxidase (PO) activities were significantly higher in Cd-exposure groups than the control groups. Superoxide dismutase (SOD) activities gradually increased in 7.25 and 14.5mgL -1 Cd groups, but in other higher Cd groups, they showed first increase and following decrease with the exposure time prolonged. Acid phosphatase (ACP) activities were induced at 48h, and then decreased, while alkaline phosphatase (AKP) activities increased gradually until 96h. Electron microscopic results showed that nucleus, mitochondria and rough endoplasm recutulum (rER) of three types of hemocytes were sensitive to acute Cd toxicity. In Cd-exposed groups, chromatin condensation, nucleus deformation and nucleus envelope rupture were noted. Additionally, mitochondrial dilation and rER degranulation were observed in Cd-treated crabs. These results suggested that immune response and organelles of hemocyte of S. henanense were impacted by Cd exposure, and the changes of these immunologic parameters reflect changes in crab immune response capability consequent to Cd exposure. © 2011 Elsevier B.V.