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Li R.,Shanxi University | Ruan W.,Shanxi Institute of Medicine and Life Science | Yao J.,Nanjing Medical University | Chen K.,Nanjing Medical University | And 3 more authors.
Gaofenzi Cailiao Kexue Yu Gongcheng/Polymeric Materials Science and Engineering | Year: 2014

An amphiphilic graft copolymer composed of γ-polyglutamic acid (γ-PGA) as the hydrophilic backbone and cholesterol as the hydrophobic segment was synthesized. The structure of cholesterol-bearing γ-PGA (γ-PGA-graft-CH) was studied by FT-IR and 1H-NMR. Amphiphilic γ-PGA-graft-CH was able to form self-assembly nanoparticles composed of an inner hydrophobic core and an outer shell of hydrophilic groups. Self-assembly nanoparticles were formed by the ultrasonic probe method. The average diameter of obtained nanoparticles is (299.6±5.4)nm at neutral pH, particle size distribution (PDI=0.17) is narrow. The experimental results also show that the formation of self-assembly nanoparticles was significantly affected by pH value of the medium as a result of the comprehensive influence of molecular charge and flexibility of γ-PGA-Graft-CH, as well as the hydrophobic interaction. The self-assembly nanoparticles of γ-PGA-Graft-CH can be used as an effective carrier of some hydrophilic or macromolecular drugs.

Zhang C.,Taiyuan University of Science and Technology | Dong Y.,Shanxi Institute of Medicine and Life Science | Zhao L.,Shanxi University
Journal of Microencapsulation | Year: 2014

In this study, a novel poly(3-hydroxybutyrate-co-3-hydroxyoctanoate) (P(HB-HO)) microparticle with an encapsulated antibiotic (azithromycin, AZI) was prepared by the electrospinning method. The resulting microparticles were evaluated for surface morphology, particle size, drug loading, encapsulation efficiency, in vitro drug-release and degradation. The in vitro cytotoxicity and in vivo pharmacokinetics were also studied. The sizes of microparticles showed a narrow monodisperse size distribution approximately from 3 to 30μm. In vitro release experiments exhibited sustained release behavior. The results of in vitro degradation tests demonstrated that the mass loss of the P(HB-HO) microparticles was 9.6% and the morphology varied greatly within 24 weeks. P(HB-HO) showed no cytotoxicity to fibroblast when incubated with blank P(HB-HO) microparticles during the tests. The in vivo pharmacokinetic study demonstrated that the microparticles exhibited longer circulation properties than free AZI. It is suggested that novel AZI-loaded P(HB-HO) microparticles can be utilized as a biodegradable and biocompatible drug delivery system. © 2014 Informa UK Ltd.

Li G.-J.,CAS Institute of Microbiology | Li G.-J.,University of Chinese Academy of Sciences | Zhao D.,CAS Institute of Microbiology | Li S.-F.,CAS Institute of Microbiology | And 3 more authors.
Mycotaxon | Year: 2013

Russula changbaiensis (subg. Tenellula sect. Rhodellinae) from the Changbai Mountains, northeast China, is described as a new species. It is characterized by the red tinged pileus, slightly yellowing context, small basidia, short pleurocystidia, septate dermatocystidia with crystal contents, and a coniferous habitat. The phylogenetic trees based on ITS1-5.8SITS2 rDNA sequences fully support the establishment of the new species. © 2013. Mycotaxon, Ltd.

Zhang C.,Taiyuan University of Science and Technology | Wu Y.,Shanxi University | Dong Y.,Shanxi Institute of Medicine and Life Science | Xu H.,Taiyuan University of Science and Technology | Zhao L.,Shanxi University
Pharmaceutical Biology | Year: 2016

Context: Doxorubicin (DOX)-loaded folate-targeted poly(3-hydroxybutyrate-co-3-hydroxyoctanoate) [P(HB-HO)] nanoparticles [DOX/FA-PEG-P(HB-HO) NPs] were prepared by the W1/O/W2 solvent extraction/evaporation method for applications in cancer treatment. However, the biodistribution, pharmacokinetics, and targeting of the nanoparticles (NPs) have not yet been studied. Objective: The biodistribution, pharmacokinetics, and targeting of DOX/FA-PEG-P(HB-HO) NPs were evaluated in female BALB/c nude mice bearing HeLa tumors. Materials and methods: Three DOX formulations were injected into the tail vein of the mice at a dosage of 5 mg/kg. At each time point, blood and various tissues were collected. All samples were then processed and analyzed by a validated high performance liquid chromatographic (HPLC) method. Results: The t1/2 values of DOX/P(HB-HO) NPs and DOX/FA-PEG-P(HB-HO) NPs were 2.7- and 3.5-times higher than that of free DOX. No significant difference (p > 0.05) was found in Cmax between the NPs and free DOX. The Tmax values of the two NPs were prolonged from 0.25 to 1 h. The AUC0-t values were 1.55- and 3.05-folds higher than that of free DOX, and MRT increased to 15.99 h for DOX/P(HB-HO) NPs and 25.14 h for DOX/FA-PEG-P(HB-HO) NPs. For DOX/FA-PEG-P(HB-HO) NPs, the DOX content in the tumors were 10.81- and 3.33-times higher than those for free DOX and DOX/P(HB-HO) NPs at 48 h, respectively.Discussion and conclusions: DOX/FA-PEG-P(HB-HO) NPs displayed reduced cardiac toxicity and improved bioavailability. Moreover, the NPs exhibited a significant extent of DOX accumulation in the tumors, thus suggesting that folate-targeted NPs could effectively transport into HeLa tumors with satisfying targeting. © 2015 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.

Yang Y.,Shanxi Institute of Medicine and Life Science | Yang Y.,University of Sichuan | Chen M.,China Pharma | Kuang Y.,University of Sichuan | And 3 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2013

A high performance liquid chromatographic method coupled with fluorometric detection has been developed for the determination of atracurium and its major metabolite laudanosine in dog plasma. The separation of atracurium and laudanosine was performed on an Agilent Eclipse Plus C18 column, and the mobile phase consisted of 0. 03 mol/L dipotassium hydrogen phosphate and acetonitrile (72: 28, v/v) at a flow rate of 1. 0 mL/min. Verapamil was used as the internal standard. The sample was extracted by dichloromethane, concentrated and dissolved in the mobile phase. The detection is performed at 240 nm for excitation and 320 nm for emission. The results showed that the linear concentration ranges of the calibration curve were 25-5 000 μg/L for atracurium (r = 0. 999 0), and 25-6 000 μg/L for laudanosine (r = 0.998 4). The recoveries were 92. 1% - 109.5%. The limits of detection were 3 μg/L for atracurium and 1 μg/L for laudanosine. The RSDs of intra-day and inter-day were less than 10%. The stability tests under various conditions have been performed. The method is specific, sensitive and accurate in the determination of atracurium and laudanosine, and also can be used for the pharmacokinetic investigations of atracurium and laudanosine in plasma.

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