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Shen J.J.,Shanxi University | Qiao Z.J.,Shanxi University | Xing T.J.,Shanxi University | Zhang L.P.,Shanxi University | And 7 more authors.
Journal of Applied Microbiology | Year: 2012

Aims: Arabidopsis thaliana l- and d-cysteine desulfhydrases (AtLCD and AtDCD) are two important H2S-generating enzymes. This study determined the effects of H2S derived from AtLCD and AtDCD on cadmium (Cd) toxicity in Escherichia coli. Methods and Results: AtLCD and AtDCD were cloned into pET28a vectors and transformed into wild-type E. coli strain BL21(DE3), named BL21(LCD) and BL21(DCD). In the induced BL21(LCD) and BL21(DCD) compared with wild type, significantly higher H2S generation rates were observed. Additionally, higher survival rates, reduced contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), decreased activities of superoxide dismutase and catalase under 220 μmol l-1 Cd stress were noted. We obtained similar results in the wild type treated with NaHS, a H2S donor. The above changes were substantially counteracted by the mixture of ammonia and pyruvic acid potassium (NH3 + C3H3KO3), a synthetic inhibitor of H2S. Conclusions: AtLCD and AtDCD catalyse the H2S production, generating an ameliorating effect against Cd-induced oxidative stress and resulting in E. coli resistance to Cd toxicity. Significance and Impact of the Study: H2S as a gasotransmitter is certified to have an ameliorating effect against Cd toxicity, thus providing information for further research regarding the role of H2S in regulating resistance to the heavy metal stress in organisms. © 2012 The Society for Applied Microbiology.

Chen Y.,Shanxi University | Wang X.,Shanxi University | He Y.,Shanxi Institute of Biology | Pan W.,Shanxi Institute of Biology | And 4 more authors.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2016

OBJECTIVE: To prepare and characterize a monoclonal antibody (mAb) against human tissue factor (hTF) with anticoagulation activity.METHODS: BALB/c mice were immunized with truncated recombinant protein (rhTF243). Hybridoma cell lines were generated from cell fusion, and screened using indirect ELISA and prothrombin time (PT). After ascites was developed in BALB/c mice, antibody titers were determined using indirect ELISA. Western blotting was performed to study the antibody specificity. Anticoagulant activity of the antibody was detected by PT assay.RESULTS: A mAb to hTF with excellent anticoagulation activity was identified. Its immunoglobulin subclass belonged to IgG1. Titer of ascites fluid was 1:200 000. Western blotting and PT analysis confirmed the specificity and anticoagulant activity of the antibody. The mAb reacted specifically to both recombinant hTF243 and natural TF on SW620 colon cancer cell surface.CONCLUSION: A hTF mAb with anticoagulation activity and high specificity has been successfully prepared.

Xu H.-Y.,Taiyuan University of Technology | Xu H.-Y.,Shanxi Institute of Biology | Wang M.-H.,Shanxi Institute of Biology | Su B.-N.,Shanxi Institute of Biology
5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 | Year: 2011

In this study the characteristics of Chromium (Cr (VI)) adsorption of anaerobic granular sludge in wastewater as well as the environmental factors that influenced on the adsorption efficiency were investigated using the static sequencing batch method in a laboratory condition. The results showed that the anaerobic granular sludge had strong and fast speed adsorption capacity for Cr (VI) adsorption in wastewater. The adsorption equilibrium could be reached in 120 min, and the maximum adsorption rate was 98.1%. The adsorption process fitted to the Pseudo-second Order Adsorption Kinetics Model. The pH value was the most important factor affecting Cr (VI) adsorption. In a neutral or light acidic condition, the adsorption rate was high. However, when the pH value was greater than 8 or less than 4, the adsorption efficiency declined. Temperature was another factor affecting adsorption but its effect was small. The amount of anaerobic granular sludge used also had a significant effect on Cr (VI) adsorption. A dose of 30 ml sludge could get the best adsorption efficiency. Our results suggest the anaerobic granular sludge is a potential Cr (VI) cleaner in wastewater. © 2011 IEEE.

Xu H.,Taiyuan University of Science and Technology | Zhang C.,Taiyuan University of Science and Technology | Su B.,Shanxi Institute of Biology | Wang M.,Shanxi Institute of Biology
Chinese Journal of Applied and Environmental Biology | Year: 2013

The adsorption capacity of anaerobic granular sludge is affected in varying degrees by its characteristics, thus the efficiency of anaerobic treatment of organic pollutants in wastewater is restricted by these characteristics too. This paper studied with a static batch method at the laboratory scale the effect of characteristics of anaerobic granular sludge on initial adsorption of organic pollutants in wastewater. The results showed that the initial organic pollutant adsorption efficiency of anaerobic granular sludge was significantly related with its specific surface area and extracellular polymers. When specific surface area increased to 178.9 m2 g-1 from 108.2 m 2 g-1 the adsorption efficiency was increased by 6.1% from 70.2% to 76.3%; the increase of extracellular polymer content raised the adsorption rate by about 6.0%; the increase of hydrophobic and Zeta potential of anaerobic granular sludge could also greatly improve the adsorption efficiency of organic pollutants by 15.6% and 10.2% respectively. The initial organic pollutant adsorption performance presented a parabolic curve with increase of the sludge particle size, settling velocity and sludge volume index of anaerobic granular sludge. The above results indicated significant effect of the characteristics of anaerobic granular sludge itself on its adsorption removal efficiency of organic pollutants in waste water. Therefore, cultivating anaerobic granular sludge with excellent adsorption performance is a key to improving anaerobic biological treatment efficiency of organic wastewater.

Guo C.,Shanxi Institute of Biology | Li J.,Shanxi Institute of Biology | Yue J.,Shanxi Institute of Biology | Yang S.,Shanxi Institute of Biology | And 2 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2013

Shanxi Province is located in the east of China's Loess Plateau. Traditional agriculture is restricted by the poor environmental conditions, water shortages and low soil fertility. The only way to develop agriculture is by adjusting the methods used to the local conditions and the development of animal husbandry. The shortage of browse and forage resources requires us to find appropriate grass species for the local conditions. Panicum virgatum L. and Galega orientalis Lam. cv. xinyin No.1 are two high yield and high quality grasses, so we introduced and cultivated them in the city of Taiyuan, Shanxi Province, China. By observing their phenophases, production, suitability, nutritional content, and so on, we found these two forage species can complete their reproductive cycle in the local climate and environmental conditions and they have many strong points such as better suitability, higher production and better quality than other species. We needed to do further study on them before introducing them as new species for Shanxi Province. We studied the photosynthetic characteristics of these two grasses in detail to determine if they could be introduced and cultivated in the Province. We analyzed the relationships between the diurnal changes of photosynthetic characteristics and environmental factors at different stages of growth. We also studied the characteristics of how leaf gas exchange indicators responded to changes in the natural environment, including especially light intensity. The findings confirm the adaptability of these two grasses and provide theoretical reference for their introduction and cultivation as well as a scientific basis for the establishment of sustainable grassland agriculture in this region.We selected different stages of growth in these two species to measure their photosynthetic and ecophysiological characteristics using a Portable LI-6400 Photosynthesis System (LI-COR, Inc., Lincoln, NE, USA) on 25 May, 6 July and 25 August 2010. The results show there were significant differences in the diurnal variations and light-response curves. (1) Xinyin No.1: The diurnal change of the net photosynthetic rate (Pn) had a bimodal pattern and had a clear midday depression. The daily averages of Pn, light use efficiency (LUE) and water use efficiency (WUE) during the branching stage were higher than in the podding stage. However, the daily averages of leaf temperature (Tl), transpiration rate (Tr), stomatal conductance (Gs) and intercellular CO2 concentration (Ci) in the podding stage were higher than in the branching stage. (2) Panicum virgatum: The diurnal change of Pn had a unimodal pattern. The daily averages of Pn and Tr in the jointing stage were higher than in the heading stage, and were also lowest during the flowering stage. The daily averages of WUE and Ci in the flowering stage were higher than in the jointing stage, which were the lowest during the heading stage. The daily averages of LUE and Tl in the heading stage were higher than in the jointing stage, and were the lowest during the flowering stage. (3) Pn was significantly positively correlated with Tr, Gs, stomatal limiting value (Ls), and photosynthetically active radiation (PAR), but significantly negatively correlated with Ci. Tr was significantly positively correlated with Gs, vapor pressure deficit (Vpdl), air temperature (Ta), Tl and PAR, but was also significantly negatively correlated with air CO2 concentration (Ca). Tl and Gs were significantly positively correlated with PAR. (4) By drawing the light response curves and by taking the relative physiological indexes into account, we could draw the conclusion P. virgatum is a heliophilic plant and Xinyin No.1 is a shade tolerant plant based on relevant evaluation criteria. Also, P. virgatum has greater adaptability to diverse environments than Xinyin No.1.

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