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Lian Y.-L.,Shanxi Institute for Food and Drug Control
Chinese Journal of New Drugs | Year: 2013

Objective: To establish a fingerprint determination method for the characterization of dihydroxydibutylether optical isomers and geometric isomers. Methods: The GC analysis was performed on an HP-CHIRAL-10B capillary chiral column with FID detector. The inlet temperature was set at 200°C. The detector temperature was set at 210°C. The column temperature was programmed from 100 to 200°C with a rate of 0.35°C·min-1 and kept at 200°C for 5 min. Results: The peaks of three isomers in component I, three isomers in component II, and two isomers in fraction III can be determined simultaneously by this method. Conclusion: The method is simple, accurate, and comprehensive for the quality control and evaluation of dihydroxydibutylether optical isomers and geometric isomers.


Yang X.-H.,Shanxi Cancer Institute | Ren L.-S.,Shanxi Cancer Institute | Wang G.-P.,Shanxi Cancer Institute | Zhao L.-L.,Shanxi Cancer Institute | And 3 more authors.
Cancer Biology and Medicine | Year: 2012

Objective: The present study aims to find a convenient, rapid, and stable method to establish bladder tumor in mice. Methods: Female Balb/C-nu-nu nude mice (or female T739 mice) were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The stylet of the 24# venous retention needles was bent in a 5° to 7° angle at a distance of 15 mm from the needlepoint to form a circle with 2.61 mm to 3.66 mm radius when the stylet is rotated. The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity. The drift angle stylet was inserted into the pipe casing slowly, rotated for fi ve times, and then pulled out. A cell suspension (0.1 mL) of approximately 1×106 T24 cells (or BTT cells) was then injected immediately. Results: A total of 60 T739 mice and 60 Balb/C-nu-nu nude mice were inoculated with BTT cells and T24 cells, respectively. The bladder tumor incidence and the average survival time of the tumor-bearing mice were 100% and (26.69±9.24) d and 100% and (34.59±9.8) d for the T739 mice and Balb/C-nu-nu nude mice, respectively. Conclusions: Using the drift angle stylet to injure the mucous membrane of the urinary bladder can establish a stable bladder transplantable tumor model in mice. © 2012 by Cancer Biology & Medicine.


Li X.-F.,Shanghai University of Traditional Chinese Medicine | Hu X.-R.,Shanghai University of Traditional Chinese Medicine | Dai Z.,National Institute for Food and Drug Control | Zhang Y.,Huawei | And 2 more authors.
Chinese Traditional and Herbal Drugs | Year: 2012

Objective: To investigate the chemical constituents from the flowers of Carthamus tinctorius. Methods: Two sesquiterpenes were isolated and purified by macroporous resin, silica gel, Sephadex LH-20, and preparative HPLC. Results: Two sesquiterpenes were isolated from the water extract in the flowers of C. tinctorius and identified as (-)-methyl dihydrophaseate (1) and (-)-(9E)-methyl dihydrophaseate (2). Conclusion: Compound 2 is a new compound named carthamate A.


Ren L.,Shanxi Medical University | Yang X.,Shanxi Medical University | Zhao L.,Shanxi Medical University | Zhang H.,Shanxi Institute for Food and Drug Control | Wang J.,Shanxi Medical University
Integrative Cancer Therapies | Year: 2016

Background. Bladder cancer is a common malignant tumor with a very high recurrence rate after surgery. Intravesical instillation can help clear up the residual tumor cells after surgery and thereby reduce the recurrence rate. Objective. To establish a bladder tumor transplantation animal model and to evaluate the inhibitory effects of a novel perfusate, Su Fu'ning Lotion (SFN), on bladder tumor. Methods. SFN was compared with several commonly used chemotherapy drugs, including mitomycin (MMC) and pirarubicin (THP) for anticancer effects on the bladder cancer cell lines T24, BTT, and BIU-87 and SFN half inhibitory concentrations (IC50) were determined after 48 hours of treatment. In addition, bladder cancer orthotopic transplantation tumor models were established in BALB/C nude mice and T739 mice, and SFN anticancer effects were assessed in vivo, with normal saline and MMC as negative and positive controls, respectively. Results. SFN, MMC, and THP were all lethal to bladder cancer cells, in vitro, with SFN and THP significantly superior to MMC. IC50 values for SFN were 13.22, 11.22, and 12.5 μg/mL on T24, BTT, and BIU-87 cells, respectively. In vivo, SFN significantly reduced the mouse bladder wet weight and prolonged the animal survival compared with controls (P <.05), suggesting that SFN significantly inhibited T24/BTT cell growth in mice. Conclusion. SFN inhibited the bladder cancer cell proliferation in vitro and in vivo and significantly prolonged the survival of mice with bladder cancer xenografts, indicating that SFN could be used as a perfusate after surgery for removal of residual bladder cancers cells. © The Author(s) 2015.


PubMed | Shanxi Medical University and Shanxi Institute for Food and Drug Control
Type: Journal Article | Journal: Integrative cancer therapies | Year: 2016

Bladder cancer is a common malignant tumor with a very high recurrence rate after surgery. Intravesical instillation can help clear up the residual tumor cells after surgery and thereby reduce the recurrence rate.To establish a bladder tumor transplantation animal model and to evaluate the inhibitory effects of a novel perfusate, Su Funing Lotion (SFN), on bladder tumor.SFN was compared with several commonly used chemotherapy drugs, including mitomycin (MMC) and pirarubicin (THP) for anticancer effects on the bladder cancer cell lines T24, BTT, and BIU-87 and SFN half inhibitory concentrations (IC50) were determined after 48 hours of treatment. In addition, bladder cancer orthotopic transplantation tumor models were established in BALB/C nude mice and T739 mice, and SFN anticancer effects were assessed in vivo, with normal saline and MMC as negative and positive controls, respectively.SFN, MMC, and THP were all lethal to bladder cancer cells, in vitro, with SFN and THP significantly superior to MMC. IC50 values for SFN were 13.22, 11.22, and 12.5 g/mL on T24, BTT, and BIU-87 cells, respectively. In vivo, SFN significantly reduced the mouse bladder wet weight and prolonged the animal survival compared with controls (P < .05), suggesting that SFN significantly inhibited T24/BTT cell growth in mice.SFN inhibited the bladder cancer cell proliferation in vitro and in vivo and significantly prolonged the survival of mice with bladder cancer xenografts, indicating that SFN could be used as a perfusate after surgery for removal of residual bladder cancers cells.


Yang X.-L.,Shanxi Institute for Food and Drug Control | Li C.,Shanxi Institute for Food and Drug Control | Li H.,Shanxi Institute for Food and Drug Control
Chinese Journal of Pharmaceutical Biotechnology | Year: 2015

To confirm whether the methods of national food safety standard for different functional health foods were appropriate and scientific by verification. According to the Chinese Pharmacopoeia 2010 edition was validated the microbiological test method of 6 types in 27 kinds of health foods. There were different level missed detection problem, about 78% when were used the methods of national food safety standard to detect pathogenic bacteria of health food. The inhibitory effect of health food on Staphylococcus aureus was the strongest, and then Shigella and Streptococcus hemolyticus and Salmonella was the last. The medium dilution method (1:20) could effectively remove the bacteriostatic action to improve the accuracy when health food had bacteriostatic action. If methods of national food safety standard were directly used to detect pathogenic bacteria of health food the risk to be missed affected the accuracy of results. The problem of microbial test method validation for health food should be concerned. © 2015, Editorial Board of Pharmaceutical Biotechnology. All right reserved.


Chen H.,PLA Fourth Military Medical University | Wang Q.F.,PLA Fourth Military Medical University | Sun X.L.,PLA Fourth Military Medical University | Luo J.,shanxi Institute for Food and Drug Control | Jiang R.,PLA Fourth Military Medical University
Mendeleev Communications | Year: 2010

Cinchona alkaloid ester derivatives were adopted to asymmetric dihydroxylation and asymmetric aminohydroxylation reactions in excellent yields and enantiomeric excesses. © 2010.


Chen Z.,Shanxi University | Chen W.,Shanxi Institute for Food and Drug Control | Li C.,University of Melbourne | Pu Y.,Sichuan Bioenvironmental Engineering Co. Chengdu | Sun H.,Shanxi University
Science of the Total Environment | Year: 2016

Erosion of denuded steep rocky slopes causes increasing losses of nitrogen and phosphorus, which is a severe problem in rocky slope protection. Thus, it is important to determine the appropriate materials that can reduce the erodibility and losses of nitrogen and phosphorus of the soil. In this paper, twenty-seven simulated rainfall events were carried out in a greenhouse, in which the substrate material was artificial soil; nine types of anionic polyacrylamide (PAM) were studied, which consisted of three molecular weight (6, 12, and 18 Mg mol-1) and three charge density (10, 20, and 30%) formulations in a 3 by 3 factorial design. The results showed that: (1) Polyacrylamide application reduced total nitrogen losses by 35.3% to 50.0% and total phosphorus losses by 34.9% to 48.0% relative to the control group. (2) The losses of total nitrogen and total phosphorus had significant correlation with the molecular weight. Besides, the losses of total phosphorus, particulate-bound phosphorus and inorganic nitrogen (NH4-N) were significantly correlated with their molecular weight and charge density. However, the losses of dissolved organic nitrogen, inorganic nitrogen (NO3-N), dissolved organic phosphorus, inorganic phosphorus (PO4-P) were non-significantly correlated with molecular weight and charge density. (3) Particulate-bound nitrogen and phosphorus were responsible for the losses of nitrogen and phosphorus during runoff events, where particulate-bound nitrogen made up 71.7% to 73.2% of total nitrogen losses, and particulate-bound phosphorus made up 82.3% to 85.2% of total phosphorus losses. (4) Polyacrylamide treatments increased water-stable aggregates content by 32.3% to 59.1%, total porosity by 11.3% to 49.0%, final infiltrative rate by 41.3% to 72.5%, and reduced soil erosion by 18.9% to 39.8% compared with the control group. Overall, the results of this study indicated that polyacrylamide application in the steep rocky slope stabilization projects could significantly reduce nutrient losses and soil erosion of substrate material. © 2016 Elsevier B.V.


PubMed | University of Melbourne, Shanxi University and Shanxi Institute for Food and Drug Control
Type: | Journal: The Science of the total environment | Year: 2016

Erosion of denuded steep rocky slopes causes increasing losses of nitrogen and phosphorus, which is a severe problem in rocky slope protection. Thus, it is important to determine the appropriate materials that can reduce the erodibility and losses of nitrogen and phosphorus of the soil. In this paper, twenty-seven simulated rainfall events were carried out in a greenhouse, in which the substrate material was artificial soil; nine types of anionic polyacrylamide (PAM) were studied, which consisted of three molecular weight (6, 12, and 18 Mg mol(-1)) and three charge density (10, 20, and 30%) formulations in a 3 by 3 factorial design. The results showed that: (1) Polyacrylamide application reduced total nitrogen losses by 35.3% to 50.0% and total phosphorus losses by 34.9% to 48.0% relative to the control group. (2) The losses of total nitrogen and total phosphorus had significant correlation with the molecular weight. Besides, the losses of total phosphorus, particulate-bound phosphorus and inorganic nitrogen (NH4-N) were significantly correlated with their molecular weight and charge density. However, the losses of dissolved organic nitrogen, inorganic nitrogen (NO3-N), dissolved organic phosphorus, inorganic phosphorus (PO4-P) were non-significantly correlated with molecular weight and charge density. (3) Particulate-bound nitrogen and phosphorus were responsible for the losses of nitrogen and phosphorus during runoff events, where particulate-bound nitrogen made up 71.7% to 73.2% of total nitrogen losses, and particulate-bound phosphorus made up 82.3% to 85.2% of total phosphorus losses. (4) Polyacrylamide treatments increased water-stable aggregates content by 32.3% to 59.1%, total porosity by 11.3% to 49.0%, final infiltrative rate by 41.3% to 72.5%, and reduced soil erosion by 18.9% to 39.8% compared with the control group. Overall, the results of this study indicated that polyacrylamide application in the steep rocky slope stabilization projects could significantly reduce nutrient losses and soil erosion of substrate material.


PubMed | Yale University, Nanjing University of Technology, Nanjing Emory Biotechnology Company and Shanxi Institute for Food and Drug Control
Type: | Journal: Chemosphere | Year: 2016

2,4-Dichlorophenoxyacetic acid (2,4-D) is widely used in agriculture as herbicide/pesticide, plantgrowth regulator and fruit preservative agent. It progressively accumulates in the environment including surface water, air and soil. It could be detected in human food and urine, which poses greatrisk to the living organisms. In the present study, we investigated the developmental toxicity of 2,4-D on zebrafish (Danio rerio) embryo. 2,4-D exposure significantly decreased both the survival rate(LC

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