Shanxi Eye Hospital

Taiyuan, China

Shanxi Eye Hospital

Taiyuan, China

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Qi H.,Baylor College of Medicine | Qi H.,Peking University | Zheng X.,Baylor College of Medicine | Zheng X.,Shanxi Eye Hospital | And 3 more authors.
Journal of Cellular Physiology | Year: 2010

Although the conjunctival fornix appears to contain the greatest proportion of stem cells, it is likely that pockets of conjunctival epithelial stem cells may also exist throughout the conjunctival epithelium. This study was to investigate the potential localization of putative stem/progenitor cells in the human bulbar conjunctival epithelium by evaluating 6 keratins and 13 molecules that have been previously proposed stem cell associated or differentiation markers. We found that cornea specific cytokeratin (CK) 3 was not expressed by the bulbar conjunctival epithelial cells. In contrast, CK4 and CK7 were expressed by the superficial cells of bulbar conjunctival epithelium. CK14 and CK15 were confined to the basal cell layer. CK19 was strongly expressed by all layers of the bulbar conjunctival epithelium. The expression patterns of molecular markers in the basal cells of human bulbar conjunctival epithelium were found to be similar to the corneal epithelium. Basal conjunctival epithelial cells strongly expressed stem cell associated markers, including ABCG2, p63, nerve growth factor (NGF) with its receptors tyrosine kinase receptor A (TrkA) and neurotrophin low-affinity receptor p75NTR, glial cell-derived neurotrophic factor (GDNF) with its receptor GDNF family receptor alpha 1 (GFRα-1), integrin β1, α-enolase, and epidermal growth factor receptor (EGFR). The differentiation associated markers nestin, E-cadherin and involucrin were not expressed by these cells. These findings indicate that the basal cells of bulbar conjunctival epithelium shares a similar expression pattern of stem cell associated markers to the corneal epithelium, but has a unique pattern of differentiation associated cytokeratin expression. © 2010 Wiley-Liss, Inc.


Wang X.,Shanghai JiaoTong University | Wang X.,Shanxi Eye Hospital | Dong J.,Shanxi Medical University | Wu Q.,Shanghai JiaoTong University
Journal of Ophthalmology | Year: 2014

Purpose. To compare anterior corneal keratometry (K) measurements taken by a dual Scheimpflug analyzer and an auto kerato-refractometer. Methods. Sixty-four normal eyes underwent keratometric measurements with both devices. The repeatability of the auto kerato-refractometer measurements was assessed by calculating the coefficient of variation (COV). The interdevice agreement was evaluated using the Bland-Altman analysis, Pearson correlation coefficient, and paired two-tailed t -test. Results. The COV of the flat K and steep K measurements taken by the auto kerato-refractometer were 0.21% and 0.29%, respectively. There were no significant differences between the steep K and average K measurements for the Topcon and Galilei devices (P = 0.475, and P = 0.137, resp.). The Galilei flat K values were lower than those of the Topcon (P = 0.002). Both of the instruments showed good agreement for all anterior corneal keratometric values. There was a significant linear correlation between the Galilei and Topcon devices for the flat K (r = 0.989, P < 0.0001), steep K (r = 0.987, P < 0.0001), and average K values (r = 0.994, P < 0.0001). Conclusions. The anterior corneal flat keratometric values were not interchangeable between the Galilei and Topcon devices. However, the measurements of the two instruments showed significant linear correlation with each other. © 2014 Xiaogang Wang et al.


Wang X.,Shanghai JiaoTong University | Wang X.,Shanxi Eye Hospital | Dong J.,Shanxi Medical University | Wu Q.,Shanghai JiaoTong University
PLoS ONE | Year: 2014

Purpose: To investigate normal anterior segment parameters and analyze the possible influencing factors using a dual Scheimpflug system. Setting: Department of Ophthalmology, Affiliated Sixth People's Hospital Shanghai Jiao Tong University, Shanghai, China. Design: A prospective observational case series. Methods: A total of 153 normal subjects (153 eyes) were studied. The anterior segment parameters, including the central corneal thickness (CCT), anterior chamber depth (ACD), pupil diameter (PD), keratoconus prediction index (KPI), simulated keratometry (SimK) values, anterior instantaneous curvature (AIC), posterior axial curvature (PAC), corneal eccentricity, total corneal power (TCP), axial curvature (AC), total corneal wavefront (TCW), high order aberration (HOA), and spherical aberration (SA), were determined using a dual Scheimpflug analyzer. Results: The CCT and ACD were both negatively correlated with age (r = -0.203, p = 0.012; r = -0.589, p<0.001). There was no significant difference in the refractive indices of AIC and SimK. Compared with the negative correlation of HOA and SA (r = -0.358, p<0.001), a positive correlation was found between TCW and HOA (r = 0.561, p<0.001). Unlike the decreased tendency of AC, the TCP increased gradually from the center to the periphery in the central 8 mm diameter. TCP showed a significant correlation with AC in the analyzed area. Conclusions: AIC and SimK provide different information in clinic, but the refractive indices of them showed no difference in this healthy study population, and age should be considered when using CCT and ACD values. © 2014 Wang et al.


Wang X.,Shanxi Eye Hospital | Dong J.,Shanxi Medical University | Wu Q.,Shanghai JiaoTong University
BMC Ophthalmology | Year: 2015

Background: Corneal biometric parameters can possibly be influenced by high myopia (HM). The influence of HM on corneal thickness (CT), epithelial thickness (ET) has not yet been clearly established. The aim of this study is to observe ET, CT and axial length (AL) differences between in normal and subjects with HMs and to investigate factors influencing the corneal biometric parameters and AL, such as age and gender. Methods: A total of 97 normal subjects (97 eyes) and 48 HM subjects (48 eyes) were included. The ET and CT of the central 6-mm diameter (17 regions) and the AL data were captured. The 17 corneal and epithelial regions were the center (1 mm radius, area a), the inner ring (2.5 mm radius, area b), the outer ring (3 mm radius, area c) and the 8 radial scan lines in eight directions (Superior (1), SN (2), Nasal (3), IN (4), Inferior (5), IT (6), Temporal (7), ST (8)) with an angle of 45° between each consecutive scan line (a, b 1-8, c 1-8). Results: The ALs were increased about 4 mm in the HMs (P < 0.001). No differences in ET were observed; in contrast, significantly thicker CTs were observed in the HMs in 16 regions except the b5 subregion. In normal group, age was negatively correlated with AL but not CCT and CET and gender was correlated with CET. In HM group, age was not correlated with CCT, AL or CET and gender was correlated with AL and CCT but not CET. Conclusions: CT was thicker in the HMs but not ET. Age and gender should be considered for AL, CT and ET in both normal and HM group. © 2015 Wang et al.; licensee BioMed Central.


News Article | November 14, 2016
Site: www.eurekalert.org

NEW YORK NY (November 14, 2016)--Columbia University Medical Center (CUMC) researchers have demonstrated that vision loss associated with a form of retinitis pigmentosa (RP) can be slowed dramatically by reprogramming the metabolism of photoreceptors, or light sensors, in the retina. The study, conducted in mice, represents a novel approach to the treatment of RP, in which the therapy aims to correct downstream metabolic aberrations of the disease rather than the underlying genetic defect. The findings were published online today in the Journal of Clinical Investigation. "Although gene therapy has shown promise in RP, it is complicated by the fact that defects in 67 genes have been linked to the disorder, and each genetic defect would require a different therapy," said study leader Stephen H. Tsang, MD, PhD, the László Z. Bitó Associate Professor of Ophthalmology, Pathology and Cell Biology, and the Institute of Human Nutrition. "Our study shows that precision metabolic reprogramming can improve the survival and function of affected rods and cones in at least one type of RP. Since many, if not most, forms of the disorder have the same metabolic error, precision reprogramming could conceivably be applied to a wide range of RP patients." RP, an inherited form of vision loss, is caused by genetic defects that lead to the breakdown and loss of rods, the photoreceptors in the retina that enable peripheral and night vision. Over time, the deterioration of rods compromises the function of cones, the color-sensing photoreceptors. People with RP start to experience vision loss in childhood, and many are blind by the time they reach adulthood. Currently, there is no cure or effective treatment for RP, which affects about 1 in 4,000 people worldwide. Rods are among the most metabolically active cells in the body. They are particularly active during periods of darkness, when they burn glucose to release energy. In an earlier paper, Dr. Tsang and his colleagues theorized that rods deteriorate in RP, in part, because they lose the daytime's ability to use glucose to rebuild the rods' outer segment (the light-absorbing portion of the photoreceptor). "We hypothesized that diseased rods could be rescued by reprogramming sugar metabolism," said Dr. Tsang. Dr. Tsang tested this hypothesis in mice with a mutation in the Pde6 gene that disrupts rod metabolism, leading to an RP-like disorder. The mice were treated so that their rods could not express Sirt6, a gene that inhibits sugar metabolism. Examination of photoreceptors with electroretinography showed that the mice had significantly greater measures of rod and cone health than untreated controls. Overall, the metabolomes (all of the metabolites found in an organism) of the treated mice had accumulated the molecules needed to build the outer segment. In addition, both rods and cones survived longer in the treated mice than in the controls. While the treatment significantly prolonged survival of the diseased rods and cones, it did not prevent their eventual death. "Our next challenge is to figure out how to extend the therapeutic effect of Sirt6 inhibition," said Dr. Tsang. "Although the treatment that was used in the mice cannot be applied directly to humans, several known Sirt6 inhibitors could be evaluated for clinical use," according to Vinit B. Mahajan, MD, PhD, a contributing researcher from the University of Iowa. The inhibitors include enzyme blockers called thiomyristoyl peptides, a common plant pigment known as quercetin, and vitexin, a substance derived from the English Hawthorn tree. Dr. Tsang noted, "Further studies are needed to explore the exciting possibility that precision metabolic reprogramming may be used to treat other forms of RP and retinal degeneration." The study is titled, "Reprogramming Sirtuin-6 Attenuates Retinal Degeneration." The other contributors are: Lijuan Zhang (CUMC, the Edward S Harkness Eye Institute, and the Shanxi Eye Hospital, affiliated with Shanxi Medical University, Xinghualing, Taiyuan, Shanxi, China), Jianhai Du (University of Washington, Seattle, WA), Sally Justus (CUMC and the Edward S Harkness Eye Institute), Chun-Wei Hsu (CUMC and the Edward S Harkness Eye Institute), Luis Bonet-Ponce (National Institutes of Health, Bethesda, MD), Wen-Hsuan Wu (CUMC and the Edward S Harkness Eye Institute), Yi-Ting Tsai (CUMC and the Edward S Harkness Eye Institute), Wei-Pu Wu (CUMC and the Edward S Harkness Eye Institute), Yading Jia (Shanxi Eye Hospital), Jimmy K. Duong (CUMC), Chyuan-Sheng Lin (CUMC), Shuang Wang (CUMC), and James B. Hurley (University of Washington). The study was supported by grants from the National Institutes of Health (5P30EY019007, R01EY018213, R01EY024698, 1R01EY026682, and R21AG050437), The National Cancer Institute Core (5P30CA013696), the Research to Prevent Blindness (RPB) Physician-Scientist Award, unrestricted funds from RPB, the Tistou and Charlotte Kerstan Foundation, the Schneeweiss Stem Cell Fund, New York State (C029572), the Foundation Fighting Blindness New York Regional Research Center Grant (C-NY05-0705-0312), Jonas Family Fund, and the Gebroe Family Foundation. The authors declare no conflicts of interest. Columbia University Medical Center provides international leadership in basic, preclinical, and clinical research; medical and health sciences education; and patient care. The medical center trains future leaders and includes the dedicated work of many physicians, scientists, public health professionals, dentists, and nurses at the College of Physicians and Surgeons, the Mailman School of Public Health, the College of Dental Medicine, the School of Nursing, the biomedical departments of the Graduate School of Arts and Sciences, and allied research centers and institutions. Columbia University Medical Center is home to the largest medical research enterprise in New York City and State and one of the largest faculty medical practices in the Northeast. The campus that Columbia University Medical Center shares with its hospital partner, NewYork-Presbyterian, is now called the Columbia University Irving Medical Center. For more information, visit cumc.columbia.edu or columbiadoctors.org.


Yang J.-H.,Shanxi Eye Hospital | Guo Z.,Shanxi Medical University | Zhang T.,Shanxi Eye Hospital | Meng X.X.,Shanxi Eye Hospital | Xie L.-S.,Shanxi Eye Hospital
Regulatory Peptides | Year: 2013

This study was designed to investigate the alterations of substance P (SP) and its correlation with apoptosis of the retinal neurons in diabetic rats. The study was carried out with diabetic rats induced by streptozotocin. Changes of SP and its mRNA were examined using enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction. The effect of restoration of SP level by capsaicin (20. mg/kg, s.c.) on the apoptosis of the retinal cells was studied. The apoptosis was evaluated by change of ratio of the apoptotic cells and caspase-3 activity in the retina.It was found that increase in apoptosis of retinal cells, by 3.5 fold of control, was accompanied by reduction of SP, by 28% in protein and 32% in the mRNA in the retina at 10. weeks of induction of diabetes, compared to the controls. Capsaicin significantly elevated endogenous SP, by 29% in the mRNA and 17% in protein in the retina, with marked inhibition of the apoptosis and the activity of caspase-3 in the diabetic rats.Induction of diabetes leads to the increase of cell apoptosis and the decrease of SP in the retina. The reduction of the endogenous SP and the increase of the cell apoptosis in the retina of the diabetic rats were reversed by pretreatment with capsaicin. Restoration of SP in the retina may be a novel option for prevention of the retinal injury during development of diabetes. © 2013 Elsevier B.V.


Zhao J.-M.,Shanxi Eye Hospital
International Journal of Ophthalmology | Year: 2011

AIM: To evaluate the effect and safety of morning vs evening once daily of fixed combinations latanoprost/timolol maleate therapy in primary open angle glaucoma or in ocular hypertensive patients. METHODS: This study was prospective, observational clinial research. All ophthalmology examinations and 24 hours IOP testing were performed at the beginning of the study of latanoprost/timolol maleate. At 1 month follow-up, 24 hours IOP was tested and recorded the results and side effects. One week after stopping treatment, this was then changed to evening dosing once daily with fixed combinations of latanoprost/timolol maleate. After 1 month follow-up, 24 hours IOP was tested, the results and side effects were recorded. The IOP and side effects in two groups were then compared. RESULTS: Thirty-two patients completed this study. There was a significant reduction at each time point in the 24-hour diurnal curve of both morning (17.3 ± 3.1mmHg) and evening (17.1 ± 2.7mmHg) dosed patients, compared to the baseline IOP (21.1 ± 3.3mmHg) (P < 0.01). When the morning and evening dosing groups were compared directly, the 6:00 time point was statistically lower with evening dosing (16.4 ± 2.3mmHg) vs morning dosing (17.9 ± 2.8mmHg) (P < 0.05). A trend was observed, which indicated greater daytime reduction with night-time dosing, whereas morning dosing tended to give lower night-time pressure. There was a significantly lower mean range of diurnal pressure with evening (3.6 ± 1.6mmHg) vs morning (4.4 ± 1.6mmHg) dosing (P < 0.05). There was no significant difference between two groups of side effects (P> 005). CONCLUSION: This study suggests that fixed combinations latanoprost/timolol maleate both given once daily in the morning and evening can effectively reduce the IOP for the 24-hour diurnal curve and are well tolerated with few side effects. There is a significantly stable 24 hour IOP lowering in evening dosed fixed combinations of latanoprost/timolol maleate.


Hou G.-P.,Shanxi Eye Hospital
International Journal of Ophthalmology | Year: 2011

AIM: To study the method and effect of using Nd:YAG laser in treating posterior capsular opacities after mydriasis. METHODS: After mydriasis posterior capsular opacities treated with Nd:YAG laser posterior capsulotomy. RESULTS: The success rate of the operation was 99.8%. The best corrected visual acuity (BCVA) was improved significantly in 97.2% cases (BCVA ≥ 0.4) by Nd:YAG laser therapy after mydriasis. CONCLUSION: After mydriasis, Nd: YAG laser posterior capsulotomy is a kind of effective, safe, simple and low-rate complication technique in treating after cataract.


Zheng X.,Cullen Eye Institute | Zheng X.,Shanxi Eye Hospital | de Paiva C.S.,Cullen Eye Institute | Li D.-Q.,Cullen Eye Institute | And 2 more authors.
Investigative Ophthalmology and Visual Science | Year: 2010

Purpose. To explore the phenomenon that corneal and conjunctival tissues subjected to desiccating stress (DS) promote Th17 differentiation by stimulating the production of Th17-inducing cytokines through a dendritic cell (DC)-mediated pathway. Methods. Experimental dry eye was created by subjecting C57BL/6 mice to desiccating environmental stress. Corneal and conjunctival explants from dry eye or control mice were cocul-tured with DCs for 24 hours before CD4+ T cells were added for an additional 4 to 7 days. Expression of Th17-associated genes in the cornea, conjunctiva, DCs, and CD4+ T cells was evaluated by real-time PCR. Cytokine concentrations in cocul-ture supernatants were measured by immunobead assay. IL-17-producing T cells were identified by ELISPOT bioassay. Results. Higher levels of IL-17A, TGF-j31, TGF-j32, IL-6, IL-23, and IL-1 j3 mRNA transcripts and TGF-/31, IL-6, and IL-1/3 protein were observed in corneal epithelium and conjunctiva from dry eye mice. DCs cocultured with epithelial explants from dry eye mice for 2 days produced higher levels of TGF-/31, IL-6, IL-23, and IL-1/3 mRNA transcripts and of TGF-/31, IL-6, and IL-1/3 protein. CD4+ T cells cocultured with DCs and epithelial explants from dry eye mice expressed increased levels of IL-17A, IL-17F, IL-22, CCL-20, and retinoic acid receptor-related orphan receptor-yt mRNA transcripts and increased IL-17A protein and number of IL-17-producing T cells (Th17 cells). Conclusions. These findings demonstrate that DS creates an environment on the ocular surface that stimulates the production of Th17-inducing cytokines by corneal and conjunctival epithelia that promote Th17 differentiation through a dendritic cell-mediated pathway. © Association for Research in Vision and Ophthalmology.


Zhou G.-H.,Shanxi Eye Hospital
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2013

Background: Retinal neovascularization disease is a group of threatening-vision eye diseases. Researches showed that cathepsin B is involved in angiogenesis. Exploring a drug which inhibit retinal blood vessels will provide the basis for the molecular mechanism of these diseases. Objective: This study was to investigate the inhibitory role of cathepsin B-RNAi-lentivirus on retinal angiogenesis. Methods: Sixty 7-day-old C57BL/6J mice were raised together with maternal mice in the closed box with the oxygen concentration of (75±2)% for 5 days to establish the retinal angiogenesis mouse models. The mice were then taken into the normal air environment for continuous raise and were randomized into 3 groups. NC-GFP-LV of 1 μl and the equal volume of cathepsin B-RNAi-lentivirus was intravitreously injected respectively in 40 eyes in the control group and the gene treatment group, and no drug was administered in the 40 eyes of the model group. The mice were sacrificed and retinas were obtained. Expression of cathepsin B protein in the retina was detected by Western blot assay (cathepsin B/β-actin). Real-time PCR was used to detect and compare the expression level of cathepsin B mRNA (2ΔΔCt). FITC-dextran was used to perform heart infusion for the retinal stretched preparation 5 days after intravitreously injection. Retinal neovascularization was examined by fluorescent angiography. Results: The expression level (2-ΔΔCt) of cathepsin B mRNA was 0.74±0.12 in the gene treatment group, showing a significant decline in comparison with 1.66±0.17 and 1.58±0.29 in the model group and control group (q = 0.746, 1.588, P < 0.01). The expression level of cathepsin B protein (cathepsin B/β-actin) in the retina was 0.64±0.06, 0.93±0.09 and 0.96±0.09 respectively in the gene treatment group, model group and control group, indicating a significant reduce in the gene treatment group (q = 0.637, 0.894, P<0.01). Distorted vessels were seen in the mice retinas of the model group with more branches and vascular anastomosis, and fluorescine leakage was exhibited under the fluorescence microscope. However, the vessels were regular with less branches and angiogenesis. Conclusions: Cathepsin B-RNAi-lentivirus can effectively inhibit oxygen-induced retinal angiogenesis in mouse. Copyright © 2013 by the Chinese Medical Association.

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