Wenli L.,Shanxi Dayi Hospital of Shanxi Academy of Medical science |
Yuan J.,Shanxi Dayi Hospital of Shanxi Academy of Medical science |
Qing Z.,Shanxi Dayi Hospital of Shanxi Academy of Medical science |
Fang L.,Shanxi Dayi Hospital of Shanxi Academy of Medical science |
And 2 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2015
Objective: To investigate the cytotoxic effect of cytotoxic lymphocytes (C TLs) activated by Tat49-57-NY-ESO-ll55-l63 sensitized dendritic cells on melanoma A-375 cells. Methods: We collected the peripheral blood (about 50 ml) from healthy volunteers and isolated mononuclear cells by using lymphocyte separation medium. The cells were treated with cytokines to produce dendritic cells and T lymphocytes. After sensitized with the Tat49-57-NY-ESO-l l55-l63 peptide, the dendritic cells were co-cultured with the T lymphocyte cells to generate antigen-specific CTLs. Phenotypes of the dendritic cells ere exa ined by flo cyto etry. The antigen-specific cytotoxic activity of the TLs against -375 elano a cells vitro was assessed by the lactate dehydrogenase (LDH) method. Human lung cancer A549 cells and leukemia K562 cells were used as controls. Results: The expression rate of CD80/CD86 in dendritic cells sensitized with Tat49-57-NY-ESO-li55.i63 was (54. 9 ± 3. 3) % significantly higher than these sensitized with NY-ESO-ll55-l63([43. 8 ± 5. 7] %P < 0. 05). There were also significant increase of CD40 expression ([42. l ± l. 9] % vs [23. 7 ±2. 8] %P <0. 05) in Tat49-57-NY-ESO-ll55-l63 sensitized dendritic cells. These results indicated that the Tat fragment (49 -57) significantly improved the cell penetrating ability of NY-ESO-ll55-l63. The T lymphocytes activated by the Tat49-57-NY-ESO-ll55-l63 sensitized DC were © 2015, Editorial office of Chinese Journal of Cancer Biotherapy. All rights reserved. Source