Shanxi Caneer Hospital
Shanxi Caneer Hospital
Shi Y.Y.,Shanxi Cardiovascular Disease Hospital |
Zhou Y.A.,Taiyuan Central Hospital |
Zhang W.H.,Shanxi Caneer Hospital |
Ma Y.X.,Taiyuan Central Hospital |
And 2 more authors.
Journal of Leukemia and Lymphoma | Year: 2016
Objective To explore the function of 5-Aza-CdR in B-cell acute lymphocytic leukemia cell line NALM-6 and its influence on the expression of microRNA (miRNA) in the cells. Methods NALM-6 was treated with different concentrations of 5-Aza-CdR. Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) test, and DNA methyltransferase (DNMT) mRNA expression level was detected by reverse transcription PCR (RT-PCR). The expression changes of miRNA were detected by miScript miRNA PCR Array chip in cells after methylation. Results NALM-6 cell growth was inhibited by different concentrations of 5-Aza-CdR processing time, reaching to the maximum inhibitory rate was (74.163±0.381)%. 5-Aza-CdR affected concentrations was inversely proportional with expression level of DNMT mRNA. After 1 000 μmol/L of 5-Aza-CdR was dealed with cell 72 h, the relative expression of DNMT-1 was reduced to 0.453 ±0.021, DNMT-3L was 0.003±0.001, DNMT-3B was 0.395±0.019. MiScript miRNA PCR array sieved out 3 miRNA (miR-184, miR-23a-3p, miR-34a-5p) associated with DNA methylation. Conclusions 5-Aza-CdR down regulates the expression of DNMT gene in NALM-6 cells, and inhibits the proliferation of cells. MiR-184, miR-23a-3p and miR-34a-5p are related to DNA methylation in the occurrence and development of B-cell acute lymphocytic leukemia. © 2016, Editorial Board of Journal of Leukemia and Lymphoma. All rights reserved.
Yang J.,Shanxi Caneer Hospital |
Yang X.,Shanxi Caneer Hospital |
Wang Y.,Shanxi Caneer Hospital |
Xin L.,Shanxi Caneer Hospital |
Song X.,Shanxi Caneer Hospital
Cancer Research and Clinic | Year: 2014
Objective: To investigate the application of three stages enhanced scan of multi-slice spiral CT for the characteristic analysis on the wall and lumen of colorectal mucous adenocarcinoma. Methods: 51 patients with colorectal mucinous adenocarcinoma who had complete pathologic diagnosis were studied. GE Discovery CT 750HD scanner was used for line scan, arterial and portal venous phase scan, and delayed scan. The lesions of intestinal wall, enteric cavity and proximal normal lumen were observed by line scan and enhanced scan, and the related quantitative value and CT value were observed. Results: The intestinal wall was uneven annular thickening or hemispherical thickening. The thickening intestinal wall showed lobular changes around the inner margin, outer margin, or without lobular changes. The hierarchical sign and cvstoids sign were observed with three layers or two layers structure after enhanced scan. Metastases of the larger lymph node, liver, ovaries were characterized similar to cyst. According to the form, the stenosis was divided into natural streamlined, irregular fixed, and irregular constrictive types. The proximal normal intestinal canal of the lesion showed occlusion, mild expansion or severe expansion. Conclusions: Walls of colorectal mucous adenocarcinoma was uneven annular thickening or hemispherical thickening, in which calcification was found, and there was features of similar to layer and cyst after enhancement. The stiff intestinal wall appeared less, and the proximal bowel obstruction was rare. Therefore, depend on these characteristics, the diagnosis of colorectal mucous adenocarcinoma can be achieved.