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Lin Q.-B.,University of Jinan | Che L.-L.,Shanxi University | Guo J.,Shanxi Academy of Analytical Science | Wang R.-Z.,Shanxi Academy of Analytical Science
Food Analytical Methods | Year: 2014

Ultrahigh-performance liquid chromatography tandem mass spectrometry with 4-chloro-3,5-dinitrobenzotrifluoride (CNBF) derivatization was developed for simultaneous determination of 20 free amino acids in Chinese jujube date. The MS/MS conditions, choice of mobile phase, the extraction process, and matrix effects were studied with a view to a method of optimization. The limits of detection for measurement of the amino acids ranged from 0.8 to 600.0 μg L-1. The correlation coefficients (r2 ≥ 0.9947) indicated good correlation between the concentrations of amino acids and the peak areas for the CNBF-derivatives. Recoveries for samples spiked at 25.0, 50.0, and 100.0 mg kg-1 ranged from 66.3 to 106.6 % (except tryptophan spiked at 25.0 and 50.0 mg kg-1, which provided recoveries of 61.2 and 60.4 %, and tyrosine spiked at three different concentrations, which provided recoveries of 54.8-58.6 %), with relative standard deviation values of less than 9.1 %. © 2013 Springer Science+Business Media New York.


Zhang M.,Shanxi University | Xie J.,Shanxi University | Wang Z.,Shanxi University | Zhao L.,Shanxi University | And 2 more authors.
Atmospheric Research | Year: 2016

Sixteen polycyclic aromatic hydrocarbons (PAHs) present in PM2. 5 were analyzed in 2012 in Taiyuan (China) using high performance liquid chromatography (HPLC) with fluorescence and ultraviolet detectors. The average daily mass concentrations of σPAHs in different seasons ranged from 10.36 ng/m3 to 215.93 ng/m3 and had strong seasonal variation, with highest values in winter and lowest in summer. Over the whole year, PAHs appeared in the following order of frequency: 4-ring > 6-ring > 5-ring > 3-ring; in winter, the percentage of 4-ring PAHs was the highest (62.68%), while in summer, the percentages of 5-ring and 6-ring PAHs (cumulative total of 50.57%) were higher. The ratio method was employed to investigate potential source categories of PAHs in PM2.5 in Taiyuan, with data suggesting that the main PAH sources are coal and wood combustion and vehicle emissions. Finally, a risk assessment of PAHs was performed based on benzo(a)pyrene equivalent (BaPeq) values and individual cancer risk ratios, with results suggesting that the toxicity of PAHs was high during winter and spring time in Taiyuan. The values of incremental lifetime cancer risk (ILCR) induced by whole year inhalation exposure(Ri) for all age groups were larger than 10-6 in spring and winter, while Ri values for male and female adults in summer and autumn were also larger than 10-6, indicating high potential carcinogenic risk. During the same season, the ILCR of adults was greater than that of other age groups and that of females was a little higher than of males. With respect to the season, the ranking of ILCR in decreasing order was as follows: winter, spring, autumn, and summer. © 2016 Elsevier B.V.


Sun Y.,Taiyuan University of Technology | Fan S.,Taiyuan University of Technology | Zhang S.,Shanxi Academy of Analytical science | Zhao D.,Taiyuan University of Technology | And 2 more authors.
Sensors and Actuators, B: Chemical | Year: 2014

A new fluorescent turn-on probe for HSO3 - bearing a benzo[e]indolium moiety was developed. The HSO3 - undergoes 1,4-addition reaction with the C-4 atom in the ethylene group, resulting in a prominent fluorescence enhancement and a fluorescent color change. The reaction could be completed in 1 min in PBS buffer, and displayed a high selectivity and sensitivity for HSO3 - against other anions, including CN-. Moreover, the practical value of the probe was confirmed by application to detect the level of HSO3 - in sugar samples. © 2013 Elsevier B.V.


Zhang S.,Shanxi University | Zhang S.,CAS Institute of Microbiology | Zhang Y.-J.,Shanxi University | Liu X.-Z.,CAS Institute of Microbiology | And 2 more authors.
Journal of Industrial Microbiology and Biotechnology | Year: 2013

Some DNA sequences in the International Nucleotide Sequence Databases (INSD) are erroneously annotated, which has lead to misleading conclusions in publications. Ophiocordyceps sinensis (syn. Cordyceps sinensis) is a fungus endemic to the Tibetan Plateau, and more than 100 populations covering almost its distribution area have been examined by us over recent years. In this study, using the data from authentic materials, we have evaluated the reliability of nucleotide sequences annotated as O. sinensis in the INSD. As of October 15, 2012, the INSD contained 874 records annotated as O. sinensis, including 555 records representing nuclear ribosomal DNA (63.5 %), 197 representing protein-coding genes (22.5 %), 92 representing random markers with unknown functions (10.5 %), and 30 representing microsatellite loci (3.5 %). Our analysis indicated that 39 of the 397 internal transcribed spacer entries, 27 of the 105 small subunit entries, and five of the 53 large subunit entries were incorrectly annotated as belonging to O. sinensis. For protein-coding sequences, all records of serine protease genes, the mating-type gene MAT1-2-1, the DNA lyase gene, the two largest subunits of RNA polymerase II, and elongation factor-1α gene were correct, while 14 of the 73 β-tubulin entries were indeterminate. Genetic diversity analyses using those sequences correctly identified as O. sinensis revealed significant genetic differentiation in the fungus although the extent of genetic differentiation varied with the gene. The relationship between O. sinensis and some other related fungal taxa is also discussed. © 2013 Society for Industrial Microbiology and Biotechnology.


Liu Y.,Shanxi University | Zhang S.,Shanxi Academy of Analytical science | Lv X.,Shanxi University | Sun Y.-Q.,Shanxi University | And 2 more authors.
Analyst | Year: 2014

A but-3-yn-2-one-based 7-diethylaminocoumarin dye was exploited as a fluorescent probe to specifically detect Cys over Hcy/GSH in pure PBS buffer. The probe itself is nonfluorescent due to the donor-excited photoinduced electron transfer (d-PET) process. The Cys-induced Michael addition- rearrangement cascade reaction leads to an amino-substituted product 3a with strong fluorescence due to inhibiting CC isomerization induced fluorescence quenching by a produced intramolecular N-H⋯O hydrogen bond. The Hcy (or GSH)-induced Michael addition reaction leads to a thiol-substituted product 2b (or 4), which lacks any intramolecular hydrogen-bonding interaction, and thus displays very poor fluorescence due to the efficient CC isomerization induced fluorescence quenching. Even in the presence of Hcy (or GSH), the probe could also detect Cys with the obvious fluorescence enhancement. Assisted by using a laser scanning confocal microscope, we demonstrated that the probe could selectively image Cys in the human renal cell carcinoma 786-0 cells. This journal is © the Partner Organisations 2014.


Lv X.,Shanxi University | Wang Y.,Shanxi University | Zhang S.,Shanxi Academy of Analytical science | Liu Y.,Shanxi University | And 2 more authors.
Chemical Communications | Year: 2014

By incorporation of a specific NO-binding group, 2-amino-3′- dimethylaminobiphenyl, into a Bodipy dye, fluorescent probe 1 was constructed, which exhibited high selectivity for NO over other ROS/RNS as well as DHA, AA and MGO. © the Partner Organisations 2014.


Lin Q.-B.,Jinan University | Lin Q.-B.,Shanxi University | Che L.-L.,Shanxi University | Guo J.,Shanxi Academy of Analytical Science | And 2 more authors.
Asian Journal of Chemistry | Year: 2014

A rapid ultrahigh-performance liquid chromatography method has been established for the simultaneous determination of 20 free amino acids in mature vinegar. The chromatographic conditions, pretreatment methods and matrix effects were optimized. 4-Chloro-3,5- dinitrobenzotrifluoride was selected as a precolumn derivatizing reagent and can readily react with both primary and secondary amines. The separation of 20 amino acids was achieved within 15 min by gradient elution mode. The detection limits for the 20 different amino acids studied ranged from 1 to 9 mg L-1. The correlation coefficient values (r2 = 0.9945) indicated good correlations between the concentrations of the compounds under investigation and their peak areas within the responding ranges. Samples spiked at 50, 80 and 100 mg L-1 showed recoveries ranging from 70.1 to 109.2 % (except for tryptophan spiked with 50 mg L -1, which showed a recovery of 59.3 %) with relative standard deviations below 5.6 %. The developed method was rapid and showed high levels of efficiency, accuracy and reproducibility that could be used effectively for the simultaneous determination of 20 free amino acids in mature vinegar.


ZHAO L.J.,Shanxi University | XIE J.F.,Shanxi University | ZHANG H.,Shanxi Academy of Analytical Science | WANG Z.T.,Shanxi University | And 3 more authors.
Journal of Agricultural Science | Year: 2015

The herbicide chlorsulfuron is commonly applied to cereals and may persist in alkaline soil long enough to damage subsequent sensitive crops. Sewage water, a potential source of the heavy metal cadmium (Cd), is used to irrigate agricultural soils in many developing countries. The current work evaluated the effects of the residual herbicide chlorsulfuron and Cd on maize seedlings, with particular attention to the mechanism of their action on plant growth. Maize seeds were planted in soil that had been sprayed with chlorsulfuron and Cd, after which residues in both seedlings and the soil were measured. The chlorsulfuron dose was correlated with the amount of residue found in seedlings but not in the soil. In all, 39 metabolites were identified in seedlings using the Automated Mass spectral Deconvolution and Identification System software program and the retention index method. The combination of chlorsulfuron and Cd significantly reduced multiple metabolites in the shikimate pathway, malic acid and citric acid production in the tricarboxylic acid cycle, and lactic acid, glucose, aspartic acid, asparagine and 3-glycerophosphoric acid production. In addition, chlorsulfuron caused an increase in multiple amino acids, including tyrosine, methionine and asparagine, and a marked decrease in caffeic and cinnamic acids (the secondary metabolites derived from the shikimate pathway and galactose). Finally, chlorsulfuron and Cd stress markedly increased shikimate acid, decreased 3-glycerophosphoric acid and caused negative correlations between the amounts of phenylalanine and tyrosine and those of quinic- and cinnamic-acid. In conclusion, chlorsulfuron and Cd did not have a synergistic effect on maize seedlings; rather, the combination of these pollutants had an antagonistic effect on some amino acids. Copyright © Cambridge University Press 2015


Li J.,Modern Medicine | Li J.,Shanxi University | Zhang S.,Shanxi Academy of Analytical science | Qin X.-M.,Modern Medicine | Li Z.-Y.,Modern Medicine
Chinese Traditional and Herbal Drugs | Year: 2015

Objective: To explore the scientific basis for Farfarae Flos baked with honey. Methods: NMR-based metabolomic approach combined with PAC, OPLS-DA, and univariate analysis was used to investigate the differences between the raw Farfarae Flos (RFF) and Farfarae Flos baked with honey (HFF). Results: Forty metabolites were identified in the NMR spectra, and the multivariate statistical results showed that RFF and HFF could be clearly separated. The levels of 1-O-ethyl-β-D-glucoside, β-glucose, sucrose, and α-glucose were higher and those of valine, aspartate, and threonine were lower in HFF compared with RFF. In light of secondary metabolites, RFF contained more chlorogenic acid and caffeic acid whereas HFF contained more tussilagone and rutin. Conclusion: The results reveal the chemical differences between RFF and HFF in a holistic way, and lay the foundation for the scientific explanation of Farfarae Flos processing. © 2015, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Zhang S.,Shanxi Academy of Analytical science
Gongneng Cailiao/Journal of Functional Materials | Year: 2014

A new type of fluorene derivative, 2-(2, 4-difluorophenyl)-7-(4-methoxyphenyl)-9, 9-dioctylfluorene(compound 1) was synthesized and characterized by elemental analysis, IR spectra and NMR Spectroscopy. Its optoelectric properties were measured by UV-Vis absorption spectra and fluorescence spectra. The results show that compound 1 has low-energy absorption bands ranging from 350 nm to 400 nm in dichloromethane solution, which can be assigned to the π-π* charge transfer transitions. The optical band gap (Eg) of compound 1 was estimated to be 3.39 eV. The title compound exhibits strong blue photoluminescence under 310 nm excitation at room temperature. The fluorescence quantum yields of compound 1 in CH2Cl2 was up to 0.70. In addition, the wavelength of the absorption and emission spectra of compound 1 depend on the polarities of the solvents. ©, 2014, Journal of Functional Materials. All right reserved.

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